Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,895 C→T 4.1% intergenic (‑148/+45) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8950C→T 4.1% 11.6 ‑0.3 ‑0.0193intergenic (‑148/+45)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (93/92):  total (98/95)
Fisher's exact test strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.93e-01

Only 100 of 208 total aligned reads displayed.

AAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGT  >  NC_014034/1734796‑1734995
                                                                                                        |                                                                                                     
aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGcc                                                                                                       >  2:197414/1‑101 (MQ=255)
aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGcc                                                                                                       >  2:2059384/1‑101 (MQ=255)
   ggCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCCGGGCAATTATGGC.AAGAATAAAATGGC.GGCCtc                                                                                                     >  1:5823/1‑100 (MQ=17)
    gCTTACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCa                                                                                                   <  2:910693/101‑1 (MQ=255)
      tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCCTCAtt                                                                                                 >  1:40369/1‑100 (MQ=255)
       gccccTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAT.ATGAAACTGC.GGCCTTCATCt                                                                                                <  2:1116824/97‑1 (MQ=18)
        aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTCC.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCtt                                                                                               <  1:3938117/101‑1 (MQ=255)
        aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.TTGAAACTGC.GGCCTTCATCtt                                                                                               <  2:4459524/101‑1 (MQ=255)
        aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCtt                                                                                               <  1:971746/101‑1 (MQ=255)
        aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCtt                                                                                               <  2:3722210/101‑1 (MQ=255)
         cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTg                                                                                              >  1:712115/1‑101 (MQ=255)
         cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTg                                                                                              <  2:2338294/101‑1 (MQ=255)
          gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGc                                                                                             <  1:3065265/101‑1 (MQ=255)
           cTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGcc                                                                                            >  1:662959/1‑101 (MQ=255)
            tGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCTGGATTTTTATGGCTAAC.AGAAAACTCC.GCCCTTCACCtcaca                                                                                            >  1:3283882/1‑97 (MQ=9)
             gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                          <  1:1548212/101‑1 (MQ=255)
              cGTCAGCCGGCGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                         <  2:456167/101‑1 (MQ=255)
              cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGTGAAATTTATGGC.AAG.ATGAAACTGC.GACCTTCATCTTGCCATa                                                                                         >  1:436418/1‑101 (MQ=17)
              cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                         >  1:2294734/1‑101 (MQ=255)
               gTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                                        <  1:4223937/101‑1 (MQ=255)
                 cAGCCGTCGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                      <  2:3681062/101‑1 (MQ=255)
                 cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                      >  2:2509379/1‑101 (MQ=255)
                 cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                      >  2:6950/1‑101 (MQ=255)
                 cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                      <  2:4051915/101‑1 (MQ=255)
                 cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                      >  1:1167391/1‑101 (MQ=255)
                 cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGA.GGCCTTCATATTGCCATAAat                                                                                      >  2:4527879/1‑101 (MQ=17)
                  aGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                                     >  2:2922739/1‑101 (MQ=255)
                  aGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                                     >  2:1428254/1‑101 (MQ=255)
                      gACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTCCATCTTGCCATAAATATccc                                                                                 >  1:295962/1‑101 (MQ=255)
                           tgacggacggggggCTCT.G.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGC.AAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                                                          >  2:18019/9‑101 (MQ=2)
                           gccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                                                           <  1:848624/101‑1 (MQ=255)
                            ccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                                                          <  1:1378546/101‑1 (MQ=255)
                             acggacggggggCTCT.G.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGC.AAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                                                                        >  1:4440965/7‑99 (MQ=2)
                              gggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                        <  1:3864913/101‑1 (MQ=255)
                                gcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCggg                                                                      <  2:3891332/101‑1 (MQ=255)
                                         cgcT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATAGTTATAGT.AAG.CTGCAACTAG.CGCCCTCAACTTGCCCTGAATATGCC.GGGGGGTGGGGTAGAGccc                                                             >  2:4166224/1‑101 (MQ=2)
                                              gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                         <  1:1021295/99‑1 (MQ=255)
                                               ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                                                        >  1:2136297/2‑101 (MQ=255)
                                               ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                                                        <  2:10326/100‑1 (MQ=255)
                                                cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa                                                       <  1:1724979/101‑1 (MQ=255)
                                                cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa                                                       >  2:1376364/1‑101 (MQ=255)
                                                 ccccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa                                                      <  1:1097123/101‑1 (MQ=255)
                                                 ccccGGCCTGCGGGCTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa                                                      <  1:2431716/101‑1 (MQ=255)
                                                  cccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAc                                                     >  2:159375/1‑101 (MQ=255)
                                                   ccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc                                                    >  1:3580067/1‑101 (MQ=255)
                                                   ccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc                                                    >  2:122683/1‑101 (MQ=255)
                                                   ccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGGGCGGGGCAGAGCCCCGCTCAAcc                                                    >  2:1020723/1‑101 (MQ=255)
                                                        cTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGa                                               <  1:1192006/101‑1 (MQ=255)
                                                         tGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt                                              <  2:2105691/101‑1 (MQ=255)
                                                         tGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt                                              <  2:1745704/101‑1 (MQ=255)
                                                             gccTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                          <  2:451418/99‑1 (MQ=255)
                                                              gcTCCCCCCGGGATATTTAGGGC.AAG.ATGAACCTGC.GGCCTTCATCTTGCGATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                         <  1:2380043/100‑1 (MQ=255)
                                                              aCTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                         <  1:2077801/101‑1 (MQ=255)
                                                              aCTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                         >  2:3647358/1‑101 (MQ=255)
                                                               cTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAg                                        <  2:2951285/101‑1 (MQ=255)
                                                               cTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAg                                        <  1:4407306/101‑1 (MQ=255)
                                                                 ccccccGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                      >  2:4093953/1‑101 (MQ=255)
                                                                 ccccccGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                      <  1:1971518/101‑1 (MQ=255)
                                                                    cccGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcg                                   <  1:2509553/101‑1 (MQ=255)
                                                                     ccGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                                  <  2:3762212/101‑1 (MQ=255)
                                                                     ccGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                                  <  1:3897627/101‑1 (MQ=255)
                                                                      cGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgcg                                 >  2:594725/1‑101 (MQ=255)
                                                                       gggATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                                >  2:908707/1‑101 (MQ=255)
                                                                       gggATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                                >  1:3845935/1‑101 (MQ=255)
                                                                       gggATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                                >  2:1611064/1‑101 (MQ=255)
                                                                       gggATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATACGCgg                                >  2:1275148/1‑101 (MQ=255)
                                                                       gggATATTTATGGC.AAG.ATGAAACTGC.GGCCATCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCAAAGATGACAAGATCCGCgg                                >  1:2629682/1‑101 (MQ=255)
                                                                          atatTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                             >  2:316229/1‑101 (MQ=255)
                                                                           tatTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                            >  2:2374884/1‑101 (MQ=255)
                                                                            atTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                           >  1:1843917/1‑101 (MQ=255)
                                                                            atTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                           >  2:1636907/1‑101 (MQ=255)
                                                                               tATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                        >  2:3442028/1‑101 (MQ=255)
                                                                               tATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                        <  1:2987816/101‑1 (MQ=255)
                                                                                aTGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                       >  2:517194/1‑101 (MQ=255)
                                                                                 tGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                      >  2:3395251/1‑101 (MQ=255)
                                                                                 tGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                      <  1:910413/101‑1 (MQ=255)
                                                                                   gC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGc                    >  2:4155559/1‑101 (MQ=255)
                                                                                    c.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCt                   <  1:1448679/101‑1 (MQ=255)
                                                                                      ggg.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGCGCGGGGCAGAGCCCCGCTCAACCGCAGATGACAAGATCCGCGGCCCGGTTTTAGCtg                  >  2:4571734/3‑101 (MQ=255)
                                                                                      aaG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                  >  2:4340347/1‑101 (MQ=255)
                                                                                      aaG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                  >  2:3809374/1‑101 (MQ=255)
                                                                                      aaG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                  >  2:2557224/1‑101 (MQ=255)
                                                                                      aaG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                  >  2:271040/1‑101 (MQ=255)
                                                                                      aaG.ATGAAACTGC.CGCCTTCCTCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                  <  1:4647421/101‑1 (MQ=255)
                                                                                        g.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat                >  2:2120534/1‑101 (MQ=255)
                                                                                           tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatga              >  2:1486800/1‑101 (MQ=255)
                                                                                           tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatga              >  1:4166847/1‑101 (MQ=255)
                                                                                           tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatga              <  2:4317973/101‑1 (MQ=255)
                                                                                            gAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg             >  2:3839722/1‑101 (MQ=255)
                                                                                             aaaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGc            >  2:3285496/1‑101 (MQ=255)
                                                                                              aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa           <  1:1114439/101‑1 (MQ=255)
                                                                                              aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa           <  2:663674/101‑1 (MQ=255)
                                                                                               aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa          >  1:2781664/1‑101 (MQ=255)
                                                                                                cTGC.AGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAg         >  2:4626156/1‑101 (MQ=255)
                                                                                                 tGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgc        >  2:284747/1‑101 (MQ=255)
                                                                                                 tGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGGTTTCGCTGATGAGCAAgc        >  2:1079233/1‑101 (MQ=255)
                                                                                                     ggCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGcc     <  1:2922968/101‑1 (MQ=255)
                                                                                                     cgCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGcc     <  1:122697/100‑1 (MQ=255)
                                                                                                      gCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGccc    >  1:2259963/1‑101 (MQ=255)
                                                                                                        cTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGt  >  2:1933177/1‑101 (MQ=255)
                                                                                                        |                                                                                                     
AAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGT  >  NC_014034/1734796‑1734995

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG