Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,769,374 C→G 96.1% P149R (CCC→GGC)  RCAP_rcc01636 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,769,3740C→G96.1% 6.8 ‑3.5 ‑0.1153P149R (CCC→CGC) RCAP_rcc01636hypothetical protein
Reads supporting (aligned to +/- strand):  new base (70/77):  ref base (0/6):  total (70/83)
Fisher's exact test strand distribution p-value = 3.15e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.57e-01

Only 100 of 156 total aligned reads displayed.

TCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTC.GGCA.GGGG.CGGTGGTC.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769284‑1769462
                                                                                               |                                                                                         
tCGGCGATGAGGACCAAATGGATGCGTCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCtt                                                                                   <  1:1413095/101‑1 (MQ=255)
 cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCttt                                                                                  <  2:508630/101‑1 (MQ=255)
 cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCttt                                                                                  <  2:1221806/101‑1 (MQ=255)
  ggCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTg                                                                                 <  1:4598498/101‑1 (MQ=255)
  ggCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTg                                                                                 >  1:1870883/1‑101 (MQ=255)
      aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGGCGGGTCGAGGTCGGGCAGGGGG.CGGAGGGCGCG.CG.CGGGCTTTGCCtt                                                                             >  1:3863694/1‑101 (MQ=255)
      aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCtt                                                                             >  1:639964/1‑101 (MQ=255)
      aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCtt                                                                             >  2:4090410/1‑101 (MQ=255)
      aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCtt                                                                             <  1:1042114/101‑1 (MQ=255)
       ttaGGACCATCTGTATGCGGCCCGCGCCGCCGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTg                                                                            <  2:1532752/99‑1 (MQ=255)
       tCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTg                                                                            <  2:2695015/101‑1 (MQ=255)
        cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGGTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTgg                                                                           <  1:2308698/101‑1 (MQ=255)
        cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTgg                                                                           >  2:1770510/1‑101 (MQ=255)
         aGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTggg                                                                          <  1:2367030/101‑1 (MQ=255)
            aCCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTc                                                                       >  2:1854549/1‑101 (MQ=255)
            aCCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTC.GC.GCGCGGGCTTTTCCTTGGGGTc                                                                       <  2:3377626/101‑1 (MQ=255)
             ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCg                                                                      <  1:546470/101‑1 (MQ=255)
              cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGa                                                                     <  2:2336117/101‑1 (MQ=255)
              cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGa                                                                     <  2:600059/101‑1 (MQ=255)
              cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTC.GCGCG.CGGGCTTTGCCTTGGGGTCGa                                                                     <  2:3183989/101‑1 (MQ=255)
              cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTC.GCGCG.CGGGCTTTGCCTTGGGGTCGa                                                                     <  2:1290170/101‑1 (MQ=255)
               aaCTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTC.GCGCG.CGGGCTTTGCCTTGGGGTCGAg                                                                    <  1:1770629/101‑1 (MQ=255)
                 cTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgc                                                                  <  2:2926775/101‑1 (MQ=255)
                 cTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgc                                                                  >  2:1890276/1‑101 (MQ=255)
                  tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcg                                                                 >  1:2687702/1‑101 (MQ=255)
                  tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGgcg                                                                 <  2:4210405/101‑1 (MQ=255)
                  tGGATGCGGCCCGCGCGGCGGAAATCGTCCCGGCTTTGGGCGCGGGCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcg                                                                 >  2:320534/1‑101 (MQ=255)
                   ggATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcgc                                                                >  1:3567797/1‑101 (MQ=255)
                     aTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCtt                                                              <  2:1935408/101‑1 (MQ=255)
                     aTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCtt                                                              <  1:2822107/101‑1 (MQ=255)
                      tGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTc                                                             <  1:4554001/101‑1 (MQ=255)
                        cGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCg                                                           >  1:24753/1‑101 (MQ=255)
                        cGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCg                                                           >  1:452902/1‑101 (MQ=255)
                         ggCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCgg                                                          >  1:1937196/1‑101 (MQ=255)
                         ggCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCgg                                                          >  2:4352100/1‑101 (MQ=255)
                          gCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCCTCCggg                                                         >  2:2329502/1‑101 (MQ=255)
                           cccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGc                                                        >  1:1712729/1‑101 (MQ=255)
                           cccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTC.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGc                                                        <  2:13503/101‑1 (MQ=255)
                               cgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCg                                                    <  1:1856763/101‑1 (MQ=255)
                               cgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGGCGAGGCGCTTCCGGGCACCg                                                    >  1:4504822/1‑101 (MQ=255)
                               cgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCg                                                    <  2:401178/101‑1 (MQ=255)
                               cgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCg                                                    <  2:4148575/101‑1 (MQ=255)
                                 cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  <  2:2166903/101‑1 (MQ=255)
                                 cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCA.GGGGTCGGTGGGCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  >  2:3878099/1‑101 (MQ=255)
                                   gcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgc                                                <  1:4425442/101‑1 (MQ=255)
                                   gcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgc                                                >  2:4647945/1‑101 (MQ=255)
                                    cggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcg                                               <  2:2819319/101‑1 (MQ=255)
                                     ggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcgc                                              <  2:4435553/101‑1 (MQ=255)
                                     ggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcgc                                              >  2:1710121/1‑101 (MQ=255)
                                     ggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcgc                                              <  1:1422121/101‑1 (MQ=255)
                                      gAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                                             >  1:1507826/1‑101 (MQ=255)
                                       aaaTCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTc                                            >  2:3672731/1‑101 (MQ=255)
                                        aaTCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTcc                                           >  1:1327067/1‑101 (MQ=255)
                                        aaTCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTcc                                           >  1:4047495/1‑101 (MQ=255)
                                          tcgtcgCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCtt                                         <  1:4482538/101‑1 (MQ=255)
                                          tcgtcgAGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCtt                                         <  1:754255/101‑1 (MQ=255)
                                             gcgcgGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAt                                      <  2:287174/100‑1 (MQ=255)
                                               gcgGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CTGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGTCACCGATGCGCTCCTTGAttt                                    <  2:4165965/101‑1 (MQ=255)
                                               gcgGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                                    >  1:1147029/1‑101 (MQ=255)
                                               gcgGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                                    <  1:1203926/101‑1 (MQ=255)
                                                cgGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                                   <  2:1870757/101‑1 (MQ=255)
                                                cgGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                                   >  1:3635515/1‑101 (MQ=255)
                                                cgGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                                   <  1:3467454/101‑1 (MQ=255)
                                                cgGCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                                   <  2:639903/101‑1 (MQ=255)
                                                 ggCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcg                                  <  1:2812212/101‑1 (MQ=255)
                                                  gCTTTGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgt                                 <  2:3700913/101‑1 (MQ=255)
                                                    tttGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtcg                               >  2:1129118/1‑101 (MQ=255)
                                                    tttGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtcg                               <  1:2028947/101‑1 (MQ=255)
                                                    tttGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtcg                               >  2:1725644/1‑101 (MQ=255)
                                                      tGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgcc                             >  2:426972/1‑101 (MQ=255)
                                                      tGGGCGCGGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgcc                             >  2:2151035/1‑101 (MQ=255)
                                                          cgcgGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCgcgc                         >  1:3034749/1‑101 (MQ=255)
                                                            cgGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                       >  1:3880384/1‑101 (MQ=255)
                                                            cgGTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                       <  1:2706219/101‑1 (MQ=255)
                                                             ggTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc                      >  1:933243/1‑101 (MQ=255)
                                                             ggTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc                      <  2:1067852/101‑1 (MQ=255)
                                                             ggTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc                      <  2:2669924/101‑1 (MQ=255)
                                                              gTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcg                     >  1:379713/1‑101 (MQ=255)
                                                              gTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcg                     <  2:2475359/101‑1 (MQ=255)
                                                              gTCGATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcg                     <  1:316542/101‑1 (MQ=255)
                                                                 gATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCgg                  >  1:3891831/1‑101 (MQ=255)
                                                                 gATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCgg                  >  2:2488498/1‑101 (MQ=255)
                                                                 gATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCgg                  <  1:124622/101‑1 (MQ=255)
                                                                 gATGTCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCgg                  >  2:1293090/1‑101 (MQ=255)
                                                                    gtcgggcaggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCt               <  2:2631914/92‑1 (MQ=255)
                                                                    gtcgggcaggggg.CGGTGGTCGCG.CG.CGGGCTTGGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCt               <  1:4018433/92‑1 (MQ=255)
                                                                     tcgggcaggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTg              <  2:3013847/93‑1 (MQ=255)
                                                                      cgggcaggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTgg             >  2:2768839/8‑101 (MQ=255)
                                                                      cgggcaggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCCTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTgg             >  1:4163124/8‑101 (MQ=255)
                                                                       gggcaggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            >  2:4456721/7‑101 (MQ=255)
                                                                       gggcaggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            <  2:3537331/95‑1 (MQ=255)
                                                                        ggcaggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtg           <  1:3768968/96‑1 (MQ=255)
                                                                         gcaggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgc          <  2:1146937/97‑1 (MQ=255)
                                                                         gcaggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgc          <  1:4090696/97‑1 (MQ=255)
                                                                          cagggtg.CGGTGGTCGCA.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGTTCTCCTTGAGTTCGTCTCCGCTCATCGCGGGCTGGtccc         >  2:792033/7‑101 (MQ=255)
                                                                             gggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGTCTGGtgccgat      <  2:3869126/101‑1 (MQ=255)
                                                                             gggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      <  2:4569477/101‑1 (MQ=255)
                                                                             gggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      <  2:554342/101‑1 (MQ=255)
                                                                             gggg.CGGTGGTC.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      >  1:1319554/1‑101 (MQ=255)
                                                                                  cGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgccg  >  2:487192/1‑101 (MQ=255)
                                                                                               |                                                                                         
TCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTC.GGCA.GGGG.CGGTGGTC.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769284‑1769462

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG