Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,770,645 G→C 100% R326P (CGG→CCG)  lpxB → lipid‑A‑disaccharide synthase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,770,6450G→C100.0% 661.3 174R326P (CGG→CCG) lpxBlipid‑A‑disaccharide synthase
Reads supporting (aligned to +/- strand):  new base (83/91):  ref base (0/0):  total (83/91)

Only 100 of 183 total aligned reads displayed.

TGACGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCGGCCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAACGACGAT  >  NC_014034/1770549‑1770741
                                                                                                |                                                                                                
   cGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGt                                                                                           <  1:1637645/101‑1 (MQ=255)
    gTGGCATATCACTAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTc                                                                                          <  2:4361283/101‑1 (MQ=255)
     tGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCg                                                                                         <  1:1651324/101‑1 (MQ=255)
      ggCATATCACGAAGCGCGCGATGCTGAACGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCgg                                                                                        <  2:2692180/101‑1 (MQ=255)
       gCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGc                                                                                       <  2:4417634/101‑1 (MQ=255)
         atatCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCg                                                                                     <  2:3046083/101‑1 (MQ=255)
         atatCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCg                                                                                     >  1:497605/1‑101 (MQ=255)
           atCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGaa                                                                                   <  1:4602841/101‑1 (MQ=255)
           atCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGaa                                                                                   >  1:373340/1‑101 (MQ=255)
            tCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGaaa                                                                                  >  2:2222221/1‑101 (MQ=255)
            tCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGaaa                                                                                  >  1:4561502/1‑101 (MQ=255)
             cACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAAc                                                                                 <  2:4230643/101‑1 (MQ=255)
              aCGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAAcc                                                                                >  2:166235/1‑101 (MQ=255)
              aCGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAAcc                                                                                <  2:3836571/101‑1 (MQ=255)
              aCGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAAcc                                                                                <  1:562911/101‑1 (MQ=255)
                gAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAAccca                                                                              >  1:3637409/1‑99 (MQ=255)
                 aaGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGAt                                                                             <  1:2448444/101‑1 (MQ=255)
                 aaGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGAt                                                                             >  2:4062410/1‑101 (MQ=255)
                    cgcgcgATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGgc                                                                          >  2:1526432/1‑101 (MQ=255)
                     gcgcgATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGgcg                                                                         <  1:3625313/101‑1 (MQ=255)
                        cgATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCgg                                                                      >  1:3799731/1‑101 (MQ=255)
                        cgATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCgg                                                                      <  1:1443802/101‑1 (MQ=255)
                           tGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGgcg                                                                   <  1:3159873/101‑1 (MQ=255)
                             ctaaTCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCt                                                                 <  1:784606/98‑1 (MQ=255)
                             cTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCt                                                                 >  1:3048493/1‑101 (MQ=255)
                             cTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCt                                                                 <  2:2670395/101‑1 (MQ=255)
                               gATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGc                                                               >  2:1727415/1‑101 (MQ=255)
                               gATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGc                                                               <  1:2473101/101‑1 (MQ=255)
                               gATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGc                                                               >  1:2068200/1‑101 (MQ=255)
                                 tCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAg                                                             <  1:2772107/101‑1 (MQ=255)
                                 tCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAg                                                             >  1:1516155/1‑101 (MQ=255)
                                     cacGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATc                                                         <  2:4342679/101‑1 (MQ=255)
                                        ggTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGt                                                      <  1:1936126/101‑1 (MQ=255)
                                        ggTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGt                                                      >  2:3279313/1‑101 (MQ=255)
                                         gTGACGGTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGtg                                                     <  2:4510236/101‑2 (MQ=255)
                                         gTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGtt                                                     <  2:400132/101‑1 (MQ=255)
                                         gTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGAGCttgt                                                     >  1:4577994/1‑98 (MQ=255)
                                          tGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTg                                                    <  1:3464516/101‑1 (MQ=255)
                                          tGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTg                                                    >  1:350105/1‑101 (MQ=255)
                                           gACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGc                                                   <  1:2842802/101‑1 (MQ=255)
                                           gACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGc                                                   >  1:2539025/1‑101 (MQ=255)
                                           gACGCTGGTCAATCTGGTGCCTGAGACCCGGGCGGGGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCCGGCGCCGCAGGCTCTGttcc                                                   >  1:143571/1‑99 (MQ=255)
                                            aCGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCg                                                  <  1:3065406/101‑1 (MQ=255)
                                            aCGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCg                                                  >  1:872437/1‑101 (MQ=255)
                                             cGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCgg                                                 <  1:3286160/101‑1 (MQ=255)
                                               cTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGa                                               <  2:1801150/101‑1 (MQ=255)
                                                 ggTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATc                                             <  2:2838885/101‑1 (MQ=255)
                                                   tCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCg                                           <  1:918964/101‑1 (MQ=255)
                                                    cAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGc                                          >  2:1183522/1‑101 (MQ=255)
                                                     aaTCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCa                                         <  2:3375347/101‑1 (MQ=255)
                                                       tCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAc                                       >  1:3412048/1‑101 (MQ=255)
                                                        cTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAcg                                      <  1:1004837/101‑1 (MQ=255)
                                                         tGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAcgc                                     >  1:1874382/1‑101 (MQ=255)
                                                         tGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCCCAAcgc                                     >  2:2319699/1‑101 (MQ=255)
                                                          ggTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAcgcc                                    >  2:739996/1‑101 (MQ=255)
                                                           gtgtCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAcgccg                                   >  2:433136/1‑101 (MQ=255)
                                                             gtCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCcgcg                                 >  2:4275320/1‑101 (MQ=255)
                                                             gtCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCcgcg                                 <  2:3930749/101‑1 (MQ=255)
                                                             gtCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCcgcg                                 <  1:2964409/101‑1 (MQ=255)
                                                                tGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCgcgg                              >  1:1457973/1‑101 (MQ=255)
                                                                tGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCgcgg                              >  1:2365277/1‑101 (MQ=255)
                                                                 gagaCCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCgcggc                             >  2:533540/1‑101 (MQ=255)
                                                                 gagaCCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCgcggc                             >  1:214894/1‑101 (MQ=255)
                                                                 gagaCCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCgcggc                             >  1:4068109/1‑101 (MQ=255)
                                                                   gaCCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGgcgc                           >  2:2050481/1‑101 (MQ=255)
                                                                   gaCCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGgcgc                           >  1:1320821/1‑101 (MQ=255)
                                                                    aCCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCa                          >  2:223908/1‑101 (MQ=255)
                                                                    aCCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCAGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCa                          >  1:3922728/1‑101 (MQ=255)
                                                                      ccGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAgg                        <  1:1089984/101‑1 (MQ=255)
                                                                      ccGGGCGGTGCCGGAATCCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAgg                        >  1:1011085/1‑101 (MQ=255)
                                                                        gggCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGtt                      >  1:3232618/1‑101 (MQ=255)
                                                                        gggCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGtt                      >  2:1043012/1‑101 (MQ=255)
                                                                        ggacGGTGCCGGAATTGCTGGGCCCGGCCTGTCGGCCGAATCCGATGGCGCGGGCGGTGGAGGATCTGTTGCGGGATCCGCAGCGCCGCGCGGCGCAGGtt                      <  2:2399334/98‑1 (MQ=255)
                                                                          gCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTc                    >  2:1272950/1‑101 (MQ=255)
                                                                             gTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGTAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGc                 <  1:199473/101‑1 (MQ=255)
                                                                               gCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCa               >  2:3648029/1‑101 (MQ=255)
                                                                               gCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCa               >  2:2767313/1‑101 (MQ=255)
                                                                               gCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCa               <  2:497557/101‑1 (MQ=255)
                                                                               gCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGCATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCa               >  1:3978291/1‑101 (MQ=255)
                                                                                ccGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCAt              <  1:1183614/101‑1 (MQ=255)
                                                                                  ggAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATgg            <  1:637040/101‑1 (MQ=255)
                                                                                  ggAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATgg            <  2:373303/101‑1 (MQ=255)
                                                                                   gAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGa           <  1:21823/101‑1 (MQ=255)
                                                                                   gAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGa           <  1:1701413/101‑1 (MQ=255)
                                                                                    aaTTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGaa          >  1:1207988/1‑101 (MQ=255)
                                                                                    aaTTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGaa          >  2:3131122/1‑101 (MQ=255)
                                                                                    aaTTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGaa          <  2:3622621/101‑1 (MQ=255)
                                                                                    aaTTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCGGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGaa          <  1:1732090/101‑1 (MQ=255)
                                                                                     aTTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGaaa         >  1:4454990/1‑101 (MQ=255)
                                                                                      ttCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGaaaa        >  2:4244453/1‑100 (MQ=255)
                                                                                      ttCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAac        <  1:3369648/101‑1 (MQ=255)
                                                                                       tCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAacg       <  1:2427776/101‑1 (MQ=255)
                                                                                       tCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAacg       <  2:1610585/101‑1 (MQ=255)
                                                                                        ccTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAacga      <  1:3618498/101‑1 (MQ=255)
                                                                                        ccTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAacga      <  2:2072460/101‑1 (MQ=255)
                                                                                        ccTCGGCCCGGCCTGTCGGCCGAAACCGAAGGCGGGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGGTTCCGCCATGGAAacga      >  2:2036415/1‑101 (MQ=255)
                                                                                         cTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAacgac     <  1:3399560/101‑1 (MQ=255)
                                                                                          tCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAacgacg    <  1:4292356/101‑1 (MQ=255)
                                                                                           cggcccggCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAAcgacga   >  2:2372020/1‑101 (MQ=255)
                                                                                           cggcccggCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCAGCCATGGAAAcgacga   >  1:1388636/1‑101 (MQ=255)
                                                                                                |                                                                                                
TGACGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCGGCCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAACGACGAT  >  NC_014034/1770549‑1770741

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG