Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,782,184 T→A 74.0% S661T (TCA→ATA)  RCAP_rcc01644 → FMN‑binding domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,782,1840T→A74.0% 168.0 ‑0.1 0.0181S661T (TCA→ACA) RCAP_rcc01644FMN‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (79/55):  ref base (27/20):  total (106/75)
Fisher's exact test strand distribution p-value = 8.65e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

TGTGCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.TCACTTGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCG.AACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTGG  >  NC_014034/1782095‑1782275
                                                                                          |                                                                                            
tgtgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCTGCTATTCCGAGTGTTTCCAGTGCTTTGACTGTGTGGCG.ATCCATgacgac                                                                                   >  2:4635127/1‑101 (MQ=255)
tgtgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCCGTGCCTTGACTGTGTGGCG.ATCCATgacgac                                                                                   >  2:1566193/1‑101 (MQ=255)
tgtgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATgacgac                                                                                   >  2:2019665/1‑101 (MQ=255)
tgtgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATgacgac                                                                                   >  1:4627569/1‑101 (MQ=255)
 gtgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACa                                                                                  <  2:3422673/101‑1 (MQ=255)
 gtgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACa                                                                                  >  1:3440071/1‑101 (MQ=255)
  tgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaa                                                                                 >  2:864645/1‑101 (MQ=255)
   gCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaaa                                                                                <  2:832374/101‑1 (MQ=255)
    ccGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACaaaa                                                                               <  1:655974/101‑1 (MQ=255)
    ccGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACaaaa                                                                               <  1:2800174/101‑1 (MQ=255)
    ccGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACaaaa                                                                               <  1:2078012/101‑1 (MQ=255)
    ccGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaaaa                                                                               >  1:3305265/1‑101 (MQ=255)
     cgcgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAg                                                                              <  1:2122691/101‑1 (MQ=255)
     cgcgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAg                                                                              <  2:3226103/101‑1 (MQ=255)
        gTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCg                                                                           <  2:3167014/101‑1 (MQ=255)
        gTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCg                                                                           >  1:4396753/1‑101 (MQ=255)
        gTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCg                                                                           >  2:2525128/1‑101 (MQ=255)
          gcgctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCt                                                                         <  2:4118120/101‑1 (MQ=255)
          gcgctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCt                                                                         >  1:3942417/1‑101 (MQ=255)
            gctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCtgt                                                                       >  1:4244238/1‑101 (MQ=255)
             ctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCtgtg                                                                      <  2:3809586/101‑1 (MQ=255)
             ctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTAGTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCtgtg                                                                      >  2:2378052/1‑101 (MQ=255)
              tgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCtgtgt                                                                     >  1:1500480/1‑101 (MQ=255)
              tgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCtgtgt                                                                     >  1:3482756/1‑101 (MQ=255)
               gcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGGGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCtgtgtg                                                                    >  1:1028764/1‑101 (MQ=255)
                cgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGc                                                                   <  2:3196349/101‑1 (MQ=255)
                 gcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGcc                                                                  <  1:4516154/101‑1 (MQ=255)
                  ccTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCt                                                                 <  1:405429/101‑1 (MQ=255)
                  ccTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCt                                                                 >  2:2470302/1‑101 (MQ=255)
                   cTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCtt                                                                <  1:1050808/101‑1 (MQ=255)
                     aTGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTg                                                              >  1:1726960/1‑101 (MQ=255)
                     aTGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTg                                                              >  1:896253/1‑101 (MQ=255)
                      tGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTgg                                                             <  1:2820407/101‑1 (MQ=255)
                      tGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTgg                                                             >  2:2176671/1‑101 (MQ=255)
                       ggCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGt                                                            <  1:1636158/101‑1 (MQ=255)
                        gcgcGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTg                                                           <  2:4652778/101‑1 (MQ=255)
                           cgATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGAGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTg                                                        >  2:3866931/1‑101 (MQ=255)
                            gATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTgg                                                       >  2:1205157/1‑101 (MQ=255)
                              tCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcg                                                     >  1:1081981/1‑101 (MQ=255)
                                aaGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcggc                                                   <  2:2146683/101‑1 (MQ=255)
                                aaGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcggc                                                   >  1:1897228/1‑101 (MQ=255)
                                 aGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGgcggcg                                                  <  1:356076/101‑1 (MQ=255)
                                   cgcACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aa                                               <  1:832589/101‑1 (MQ=255)
                                   cgcACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aa                                               <  2:584280/101‑1 (MQ=255)
                                    ncACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCNNnn.nan                                              >  1:1007448/2‑100 (MQ=255)
                                     cACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaac                                             >  2:4077388/1‑99 (MQ=255)
                                     cACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaac                                             <  2:3440499/101‑3 (MQ=255)
                                       ccGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaacga                                           >  2:3243424/1‑97 (MQ=255)
                                        cGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaacgaa                                          <  1:3790159/101‑6 (MQ=255)
                                         ggCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGaaa                                          >  2:2730482/1‑101 (MQ=255)
                                          gCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAg                                         >  1:3088433/1‑101 (MQ=255)
                                          gCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAg                                         <  1:1845330/101‑1 (MQ=255)
                                           cGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAgg                                        <  1:3600484/101‑1 (MQ=255)
                                            gagaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGc                                       >  2:102171/1‑101 (MQ=255)
                                            gagaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGc                                       >  1:3413733/1‑101 (MQ=255)
                                             agaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCt                                      >  2:257100/1‑101 (MQ=255)
                                              gaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTc                                     <  1:992182/101‑1 (MQ=255)
                                               aTCCGCTATTCCTAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCg                                    >  1:474731/1‑101 (MQ=255)
                                                 ccGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCggg                                  >  1:4565862/1‑101 (MQ=255)
                                                    cTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCAt                               >  1:2703026/1‑101 (MQ=255)
                                                    cTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCAt                               <  2:4335484/101‑1 (MQ=255)
                                                     tATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATg                              <  1:2730680/101‑1 (MQ=255)
                                                      aTTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGa                             >  2:2768690/1‑101 (MQ=255)
                                                       ttCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGaa                            >  1:1938435/1‑101 (MQ=255)
                                                       tgccGAGTGTTTCCAGGGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTTGCGGCGAAACGAAAGGCTCGGGCATGaa                            <  2:3128792/99‑1 (MQ=255)
                                                        tCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAg                           >  1:1234665/1‑101 (MQ=255)
                                                         ccGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgc                          >  1:1765904/1‑101 (MQ=255)
                                                         ccGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgc                          >  2:1049856/1‑101 (MQ=255)
                                                          cGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcg                         <  1:940565/101‑1 (MQ=255)
                                                            aGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggc                       <  1:4200978/101‑1 (MQ=255)
                                                            aGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggc                       >  1:127459/1‑101 (MQ=255)
                                                             gtgtTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcg                      <  2:4470037/101‑1 (MQ=255)
                                                                tttCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcggcg                   <  2:1500371/101‑1 (MQ=255)
                                                                 ttCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGcggcggcgg                  >  2:4134707/1‑101 (MQ=255)
                                                                 ttCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGcggcggcgg                  >  1:1534943/1‑101 (MQ=255)
                                                                  tCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGcggcggcggg                 >  1:1891524/1‑100 (MQ=255)
                                                                  tCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGGGGCGGt                 >  1:4600979/1‑101 (MQ=255)
                                                                  tCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGGGGt                 >  1:4432104/1‑101 (MQ=255)
                                                                   ccAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGtt                >  2:2853477/1‑101 (MQ=255)
                                                                   ccAGTGCCTTGACTGTGTGGCG.AGCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGtt                >  2:793467/1‑101 (MQ=255)
                                                                    cAGTGCTGTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGttt               <  2:4635925/101‑1 (MQ=255)
                                                                    cAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGgtt               >  2:3308378/1‑101 (MQ=255)
                                                                     aGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTc              >  1:2552698/1‑101 (MQ=255)
                                                                     aGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTc              <  1:3308196/101‑1 (MQ=255)
                                                                      gTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCt             <  2:2000674/101‑1 (MQ=255)
                                                                      gTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCt             <  1:3416479/101‑1 (MQ=255)
                                                                        gCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGc           <  1:131841/101‑1 (MQ=255)
                                                                          cTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAg         >  1:4106818/1‑101 (MQ=255)
                                                                          cTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGctg         >  2:3014465/1‑99 (MQ=255)
                                                                          cTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAg         <  1:1566297/101‑1 (MQ=255)
                                                                           ttGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGa        <  1:4086946/101‑1 (MQ=255)
                                                                           ttGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGAGGGGGTTTCTGCAGa        >  1:725982/1‑101 (MQ=255)
                                                                            tGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTTTGCAGAt       >  1:3693111/1‑101 (MQ=255)
                                                                            tGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGAt       <  2:1081892/101‑1 (MQ=255)
                                                                             gACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATc      <  2:3627244/101‑1 (MQ=255)
                                                                             gACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATc      >  2:1734545/1‑101 (MQ=255)
                                                                              aCTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCg     <  1:810375/101‑1 (MQ=255)
                                                                              aCTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCg     <  1:292578/101‑1 (MQ=255)
                                                                                tgtgtgGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTg   <  1:2001537/101‑1 (MQ=255)
                                                                                 gtgtgGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTgg  <  2:4666299/101‑1 (MQ=255)
                                                                                          |                                                                                            
TGTGCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.TCACTTGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCG.AACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTGG  >  NC_014034/1782095‑1782275

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG