Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,786,541 C→T 2.1% A225A (GCC→GCT RCAP_rcc01648 → ABC transporter permease

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,786,5410C→T 2.1% 2.1 0.0 ‑0.0192A225A (GCC→GCTRCAP_rcc01648ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (88/99):  total (91/101)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.14e-01

Only 100 of 204 total aligned reads displayed.

TGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.GG.CCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATCC  >  NC_014034/1786444‑1786641
                                                                                                   |                                                                                                    
tgagggcGCATCTGGTCGCCGCGGCGCGGGCGCGGATTTCTCTGATCTGGAAGGACGTGCTGGAGGTCGAATTTATAGG.CCGCTCGAACGGGATCGGCTtt                                                                                                    >  2:4599558/7‑101 (MQ=255)
tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGCTCTGGATGGTCGTGCTGGGGGTCGAATTTCTGGG.CGGCTCGAACGGGATGGGCTtt                                                                                                    >  1:3874596/1‑101 (MQ=255)
 gATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGGCTttc                                                                                                   <  1:400991/101‑2 (MQ=255)
  aTGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCg                                                                                                 >  2:1589989/1‑101 (MQ=255)
   tGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGt                                                                                                <  1:574982/101‑1 (MQ=255)
    gCCGCAGCTGATCGCCGCGGCGGGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTa                                                                                               <  1:2497725/101‑1 (MQ=255)
     ccGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTAt                                                                                              <  1:4348441/101‑1 (MQ=255)
     ccGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTAt                                                                                              >  1:1756325/1‑101 (MQ=255)
      cGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATc                                                                                             >  1:2864958/1‑101 (MQ=255)
      cGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATc                                                                                             >  2:3613631/1‑101 (MQ=255)
       gCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATcc                                                                                            >  2:1386165/1‑101 (MQ=255)
       gCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATcc                                                                                            <  1:1302096/101‑1 (MQ=255)
        cATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCa                                                                                           >  1:3538477/1‑101 (MQ=255)
         aTCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCAt                                                                                          >  2:2769746/1‑101 (MQ=255)
          tCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATc                                                                                         >  1:1717473/1‑101 (MQ=255)
            tGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTg                                                                                       <  2:147576/101‑1 (MQ=255)
              aTCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGaa                                                                                     <  2:587309/101‑1 (MQ=255)
               tCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAt                                                                                    <  1:1186362/101‑1 (MQ=255)
                cgccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.GTTTCGTATCCATCTGAAtt                                                                                   >  2:1648624/1‑101 (MQ=255)
                 gccgcGGCGCGGGCGCGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTGCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAttt                                                                                  <  2:3138788/101‑1 (MQ=255)
                  ccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTc                                                                                 <  2:3978275/101‑1 (MQ=255)
                    gcggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCa                                                                               <  1:4191734/101‑1 (MQ=255)
                     cggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAg                                                                              >  1:164299/1‑101 (MQ=255)
                      ggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGa                                                                             >  1:111503/1‑101 (MQ=255)
                         gcgggcggGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGt                                                                          >  2:3051689/1‑101 (MQ=255)
                          cgggcgggGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGtt                                                                         <  1:1917359/101‑1 (MQ=255)
                           gggcggggCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTc                                                                        <  2:2104935/101‑1 (MQ=255)
                            ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                       <  1:618813/101‑1 (MQ=255)
                            ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                       <  2:2864742/101‑1 (MQ=255)
                            ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                       <  1:4036129/101‑1 (MQ=255)
                            ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                       <  1:1648735/101‑1 (MQ=255)
                             gcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGa                                                                      >  1:1549023/1‑101 (MQ=255)
                             gcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGa                                                                      <  2:126433/101‑1 (MQ=255)
                              cggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                     <  2:4111368/95‑1 (MQ=255)
                                gggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAt                                                                   >  1:330371/5‑101 (MQ=255)
                                gggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAt                                                                   >  2:115600/5‑101 (MQ=255)
                                  gctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTg                                                                 <  1:2328813/99‑1 (MQ=255)
                                  gctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTg                                                                 <  1:4420274/99‑1 (MQ=255)
                                   gTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGc                                                                <  1:510339/101‑1 (MQ=255)
                                   ctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGc                                                                <  2:150242/100‑1 (MQ=255)
                                    tttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGcc                                                               <  1:1224985/101‑1 (MQ=255)
                                    tttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGcc                                                               >  1:219495/1‑101 (MQ=255)
                                     ttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCg                                                              <  2:2024639/101‑1 (MQ=255)
                                       cGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAg                                                            >  1:1808594/1‑101 (MQ=255)
                                       cGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAg                                                            >  1:2034149/1‑101 (MQ=255)
                                       cGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAg                                                            >  2:4576669/1‑101 (MQ=255)
                                         cTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGt                                                          <  1:1659385/101‑1 (MQ=255)
                                          tGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTg                                                         >  1:1241349/1‑101 (MQ=255)
                                          tGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTg                                                         >  1:2168056/1‑101 (MQ=255)
                                           gATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGc                                                        >  2:1101810/1‑101 (MQ=255)
                                            aTCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCt                                                       <  2:2619572/101‑1 (MQ=255)
                                             tCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTc                                                      <  2:1864809/101‑1 (MQ=255)
                                             tCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.GGCCCGCTCGAACGGGTTCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTc                                                      <  1:1457533/101‑1 (MQ=255)
                                               tGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGc                                                    >  1:2656795/1‑101 (MQ=255)
                                                 gAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCt                                                  <  2:2377991/101‑1 (MQ=255)
                                                  aaGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTg                                                 >  2:2197767/1‑101 (MQ=255)
                                                  aaGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTg                                                 <  1:223133/101‑1 (MQ=255)
                                                  aaGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTg                                                 <  1:1838061/101‑1 (MQ=255)
                                                       cGTGCTGGTGGTGGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcgg                                            <  1:2040116/101‑1 (MQ=255)
                                                        gTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                           >  1:4447034/1‑101 (MQ=255)
                                                         tGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggcg                                          <  1:1590094/101‑1 (MQ=255)
                                                         tGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGATGCTCGCCTGggcggcg                                          >  2:4186090/1‑101 (MQ=255)
                                                          gCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggcgg                                         >  2:2219407/1‑101 (MQ=255)
                                                           cTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggcggc                                        >  2:211017/1‑101 (MQ=255)
                                                             ggtggtCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCtt                                      <  1:381173/101‑1 (MQ=255)
                                                             ggtggtCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCtt                                      <  2:585493/101‑1 (MQ=255)
                                                             ggtggtCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCtt                                      <  1:1268086/101‑1 (MQ=255)
                                                              gtggtCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCttt                                     <  2:1801958/101‑1 (MQ=255)
                                                               tggtCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTc                                    <  2:3225108/101‑1 (MQ=255)
                                                                 gtCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGt                                  <  1:4566233/101‑1 (MQ=255)
                                                                  tCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTc                                 <  2:3302691/101‑1 (MQ=255)
                                                                   cGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCt                                <  1:3051933/101‑1 (MQ=255)
                                                                    gAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTg                               >  2:4264765/1‑101 (MQ=255)
                                                                    gAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTg                               >  2:778279/1‑101 (MQ=255)
                                                                     taTTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGc                              <  1:3448893/100‑1 (MQ=255)
                                                                      aTTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGca                             >  2:3448639/1‑100 (MQ=255)
                                                                       tttCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGt                            >  2:1767508/1‑101 (MQ=255)
                                                                       tttCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGt                            >  1:989470/1‑101 (MQ=255)
                                                                        ttctggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTg                           <  1:1344271/96‑1 (MQ=255)
                                                                         tctggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGa                          >  2:2649542/5‑101 (MQ=255)
                                                                         tctggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGa                          >  1:1032025/5‑101 (MQ=255)
                                                                          ctggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGAt                         <  2:511695/98‑1 (MQ=255)
                                                                           tggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                        >  2:127397/3‑101 (MQ=255)
                                                                           tggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                        >  1:4660877/3‑101 (MQ=255)
                                                                           tggg.CCGCTCGAACGGGATCGG.CTTTCGTAACCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                        >  1:4151675/3‑101 (MQ=255)
                                                                             gg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCt                      >  2:1906459/1‑101 (MQ=255)
                                                                              g.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTc                     <  2:1900673/101‑1 (MQ=255)
                                                                                     cGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTc               <  2:3694036/101‑1 (MQ=255)
                                                                                      gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg              >  2:385410/1‑101 (MQ=255)
                                                                                      gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg              <  2:1214171/101‑1 (MQ=255)
                                                                                       aaCGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGa             <  1:444097/101‑1 (MQ=255)
                                                                                        aCGGGATCGG.CTTTCGTACCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGaa            <  2:3399580/101‑1 (MQ=255)
                                                                                         cGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAAt           <  2:3531467/101‑1 (MQ=255)
                                                                                          gggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATg          >  1:1623451/1‑101 (MQ=255)
                                                                                          gggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATg          >  2:1663420/1‑101 (MQ=255)
                                                                                          gggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATg          <  1:1008943/101‑1 (MQ=255)
                                                                                             aTCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCt       >  2:3780080/1‑101 (MQ=255)
                                                                                             aTCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCt       >  1:3713613/1‑101 (MQ=255)
                                                                                                   cTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATcc  >  2:3508050/1‑101 (MQ=255)
                                                                                                   cTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATcc  >  2:713275/1‑101 (MQ=255)
                                                                                                   |                                                                                                    
TGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.GG.CCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATCC  >  NC_014034/1786444‑1786641

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG