Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,794,301 C→G 88.4% A398G (GCG→GGC)  exaA2 → quinoprotein ethanol dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,794,3010C→G88.4% 48.0 ‑1.9 0.0172A398G (GCG→GGG) exaA2quinoprotein ethanol dehydrogenase
Reads supporting (aligned to +/- strand):  new base (68/84):  ref base (5/15):  total (73/99)
Fisher's exact test strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

TGAAGAACATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GCGGCCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGGCTCTTCTAT  >  NC_014034/1794205‑1794395
                                                                                                 |                                                                                              
    gagcATCACCTGGGCCAGCGGCGTCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAgg                                                                                        <  2:788523/98‑1 (MQ=255)
    gaaCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAgg                                                                                        >  1:1395972/1‑101 (MQ=255)
    gaaCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAgg                                                                                        >  1:3205550/1‑101 (MQ=255)
    gaaCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAgg                                                                                        >  2:1322316/1‑101 (MQ=255)
     aataTCACCTCCGCCAAGGGCATCGACGAAACCGGCCGTGCGATCTAGAACGAGGATAACCGCCCTGGTGCCCCCGATGCCGTGGATGAA.GGAGGCAAggc                                                                                       >  2:3076828/4‑100 (MQ=255)
      aCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGc                                                                                      <  1:1451695/101‑1 (MQ=255)
        aTCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCca                                                                                    <  1:2517057/101‑1 (MQ=255)
        aTCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCca                                                                                    <  2:4172840/101‑1 (MQ=255)
        aTCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCACCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCca                                                                                    <  2:2380079/101‑1 (MQ=39)
         tCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCcag                                                                                   >  2:3578683/1‑101 (MQ=255)
          cACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCcagc                                                                                  >  1:1992162/1‑101 (MQ=255)
            ccTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCcagcag                                                                                <  2:363988/101‑1 (MQ=255)
            ccTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCcagcag                                                                                >  2:738822/1‑101 (MQ=255)
             cTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGa                                                                               <  2:3910948/101‑1 (MQ=255)
              tGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGAt                                                                              >  1:1813847/1‑101 (MQ=255)
              tGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGAt                                                                              >  1:4473793/1‑101 (MQ=255)
               gggCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGAGGCCGAA.GGCGGCAAGGGCCAGCAGATc                                                                             >  2:2765282/1‑101 (MQ=255)
                gggcAAGGGCATCGATGAAACCGGCGGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCt                                                                            <  2:445118/98‑1 (MQ=255)
                ggCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCt                                                                            <  2:3924545/101‑1 (MQ=255)
                 gCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCtt                                                                           <  2:3647270/101‑1 (MQ=255)
                 gCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCtt                                                                           >  2:3089507/1‑101 (MQ=255)
                  ccAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCttt                                                                          <  1:767773/101‑1 (MQ=255)
                  ccAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCttt                                                                          >  1:2461091/1‑101 (MQ=255)
                   cAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTg                                                                         <  2:1809621/101‑1 (MQ=255)
                   cAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTg                                                                         <  2:2300361/101‑1 (MQ=255)
                   cAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTg                                                                         >  2:63116/1‑101 (MQ=255)
                    aaGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGc                                                                        <  1:2184249/101‑1 (MQ=255)
                    aaGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGACCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGc                                                                        >  2:1622747/1‑101 (MQ=255)
                       ggCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGt                                                                     <  2:397836/101‑1 (MQ=255)
                          aTCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGcc                                                                  >  2:1103230/1‑101 (MQ=255)
                            cGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCt                                                                <  1:864307/101‑1 (MQ=255)
                             gATGAACCCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTc                                                               <  2:3396593/101‑1 (MQ=255)
                             gATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTc                                                               <  1:2356313/101‑1 (MQ=255)
                             gATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTc                                                               <  2:2311113/101‑1 (MQ=255)
                              aTGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCg                                                              <  1:3358186/101‑1 (MQ=255)
                               tGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGt                                                             >  1:2821143/1‑101 (MQ=255)
                                gAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGtt                                                            <  1:727100/101‑1 (MQ=255)
                                  aaCCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTcc                                                          >  1:1959195/1‑101 (MQ=255)
                                     cGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTgg                                                       <  1:4404564/101‑1 (MQ=255)
                                     cGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTgg                                                       >  2:1231433/1‑101 (MQ=255)
                                      ggCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTggg                                                      >  2:2354038/1‑101 (MQ=255)
                                      ggCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTggg                                                      >  1:4619728/1‑101 (MQ=255)
                                       gCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggc                                                     <  1:164948/101‑1 (MQ=255)
                                       gCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggc                                                     <  1:2138065/101‑1 (MQ=255)
                                       gCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggc                                                     >  1:2716563/1‑101 (MQ=255)
                                          gTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggcggc                                                  >  2:613414/1‑101 (MQ=255)
                                          gTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggcggc                                                  >  2:148685/1‑101 (MQ=255)
                                          gTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggcggc                                                  >  1:3680545/1‑101 (MQ=255)
                                              gATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGa                                              <  1:3812510/101‑1 (MQ=255)
                                              gATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGa                                              >  1:1296360/1‑101 (MQ=255)
                                              gATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGa                                              <  1:2366788/101‑1 (MQ=255)
                                               aTCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGaa                                             >  1:3546776/1‑101 (MQ=255)
                                                   aCAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTgg                                         <  2:867593/101‑1 (MQ=255)
                                                     aaCGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGAt                                       >  2:2932858/1‑101 (MQ=255)
                                                       cGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGc                                     >  1:1875940/1‑101 (MQ=255)
                                                        gaggagAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGNGGCAAGAACTGGATGcc                                    >  2:892291/1‑101 (MQ=255)
                                                        gaggagAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGcc                                    <  2:692375/101‑1 (MQ=255)
                                                         aggagAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAAATGGATGCCg                                   >  1:1836854/1‑101 (MQ=255)
                                                           gagaACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCGTGGGCCGCAAGAACTGGATGCCGAt                                 >  2:3338445/1‑101 (MQ=255)
                                                           gagaACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGAt                                 <  2:2081390/101‑1 (MQ=255)
                                                             gaACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGTTGCCGATgg                               >  2:541755/1‑101 (MQ=255)
                                                              aaCCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGCTCCTGGGCGGCAAGAACTGGATGCCGATGGc                              <  1:4131687/101‑1 (MQ=255)
                                                                ccGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCt                            <  1:2312688/101‑1 (MQ=255)
                                                                 cGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTa                           <  2:1848860/101‑1 (MQ=255)
                                                                  gCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTAc                          <  2:1610158/101‑1 (MQ=255)
                                                                  gCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTAc                          <  2:2002475/101‑1 (MQ=255)
                                                                  gCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTAc                          <  2:609801/101‑1 (MQ=255)
                                                                   ccccGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACa                         <  2:3205307/101‑1 (MQ=255)
                                                                   ccccGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACa                         <  1:1166406/101‑1 (MQ=255)
                                                                   ccccGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACa                         >  1:2599239/1‑101 (MQ=255)
                                                                   ccccGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACa                         <  1:3392446/101‑1 (MQ=255)
                                                                    nccGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAg                        <  1:3495373/100‑1 (MQ=255)
                                                                    cccGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAg                        >  2:2228811/1‑101 (MQ=255)
                                                                     ccGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGc                       <  2:4361787/101‑1 (MQ=255)
                                                                     ccGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGc                       >  2:2563000/1‑101 (MQ=255)
                                                                     ccGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGc                       <  1:1361261/101‑1 (MQ=255)
                                                                      cGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGcc                      <  2:1724369/101‑1 (MQ=255)
                                                                      cGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGcc                      >  2:3630236/1‑101 (MQ=255)
                                                                       ggTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCa                     >  2:786638/1‑101 (MQ=255)
                                                                       ggTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCa                     <  2:2637991/101‑1 (MQ=255)
                                                                        gTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAg                    <  1:2254605/101‑1 (MQ=255)
                                                                         tGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGa                   <  1:3671193/101‑1 (MQ=255)
                                                                         tGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGa                   <  1:575667/101‑1 (MQ=255)
                                                                          gCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGAc                  >  1:3114686/1‑101 (MQ=255)
                                                                           cccccGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGAcc                 <  1:1327164/101‑1 (MQ=255)
                                                                           cccccGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGAcc                 >  1:2872366/1‑101 (MQ=255)
                                                                           cccccGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGAcc                 <  2:1949116/101‑1 (MQ=255)
                                                                            ccccGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCa                >  1:4136177/1‑101 (MQ=255)
                                                                             cccGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGATCTGGATGCCGATGGCCTACAGCCAGACCAc               <  2:984483/101‑1 (MQ=255)
                                                                               cGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgg             >  2:2825263/1‑101 (MQ=255)
                                                                               cGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgg             >  2:133987/1‑101 (MQ=255)
                                                                                 aTGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgggc           <  1:602112/101‑2 (MQ=255)
                                                                                  tGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgggcc          >  1:2674179/1‑99 (MQ=255)
                                                                                      gcgGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtctt      <  2:1294398/101‑1 (MQ=255)
                                                                                       cgGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtcttc     <  1:647735/101‑1 (MQ=38)
                                                                                       cgGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtcttc     >  1:2492465/1‑101 (MQ=38)
                                                                                       cgGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtcttc     >  1:2411577/1‑101 (MQ=38)
                                                                                          ccGAA.GGCGGCAAGGGCCAGCAGATCTTTTCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCTCTTCTAt  >  1:4051038/1‑101 (MQ=37)
                                                                                          ccGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCTCTTCTAt  <  2:2795865/101‑1 (MQ=38)
                                                                                          ccGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCTCTTCTAt  <  2:177680/101‑1 (MQ=38)
                                                                                                 |                                                                                              
TGAAGAACATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GCGGCCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGGCTCTTCTAT  >  NC_014034/1794205‑1794395

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG