Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,794,302 G→C 88.3% A398A (GCG→GGC)  exaA2 → quinoprotein ethanol dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,794,3020G→C88.3% 48.0 ‑2.3 0.0172A398A (GCG→GCC) exaA2quinoprotein ethanol dehydrogenase
Reads supporting (aligned to +/- strand):  new base (68/83):  ref base (5/15):  total (73/99)
Fisher's exact test strand distribution p-value = 9.85e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

GAACATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GCGGCCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGGCTCTTCTAT  >  NC_014034/1794209‑1794395
                                                                                                |                                                                                             
gagcATCACCTGGGCCAGCGGCGTCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAgg                                                                                        <  2:788523/98‑1 (MQ=255)
gaaCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAgg                                                                                        >  2:1322316/1‑101 (MQ=255)
gaaCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAgg                                                                                        >  1:1395972/1‑101 (MQ=255)
gaaCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAgg                                                                                        <  2:2593349/101‑1 (MQ=255)
gaaCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAgg                                                                                        >  1:3205550/1‑101 (MQ=255)
 aaCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAggg                                                                                       >  2:1734010/1‑101 (MQ=255)
 aaCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAggg                                                                                       >  2:3967843/1‑101 (MQ=255)
  aCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGc                                                                                      <  1:1451695/101‑1 (MQ=255)
    aTCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCca                                                                                    <  1:2517057/101‑1 (MQ=255)
    aTCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCca                                                                                    <  2:2718580/101‑1 (MQ=255)
    aTCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCACCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCca                                                                                    <  2:2380079/101‑1 (MQ=39)
      cACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCcagc                                                                                  >  1:1992162/1‑101 (MQ=255)
        ccTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCcagcag                                                                                <  2:363987/101‑1 (MQ=255)
          tGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGAt                                                                              >  2:3644163/1‑101 (MQ=255)
          tGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGAt                                                                              >  1:4473793/1‑101 (MQ=255)
          tGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGAt                                                                              >  1:1813847/1‑101 (MQ=255)
           gggCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATc                                                                             >  2:230304/1‑101 (MQ=255)
            gggcAAGGGCATCGATGAAACCGGCGGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCt                                                                            <  2:445118/98‑1 (MQ=255)
            ggCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AAGGCGG.CAAGGGCCAGCAGATCt                                                                            <  2:3924545/101‑1 (MQ=255)
            ggCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AAGGCGG.CAAGGGCCAGCAGATCt                                                                            <  2:1786886/101‑1 (MQ=255)
            ggCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCt                                                                            >  2:2010497/1‑101 (MQ=255)
             gCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCtt                                                                           >  2:707900/1‑101 (MQ=255)
              ccAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCttt                                                                          >  1:2461091/1‑101 (MQ=255)
              ccAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCttt                                                                          <  1:767773/101‑1 (MQ=255)
               cAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AAGGCGG.CAAGGGCCAGCAGATCTTTg                                                                         <  2:2300361/101‑1 (MQ=255)
               cAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AAGGCGG.CAAGGGCCAGCAGATCTTTg                                                                         <  2:1287375/101‑1 (MQ=255)
               cAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTg                                                                         >  2:63116/1‑101 (MQ=255)
                aaGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AAGGCGG.CAAGGGCCAGCAGATCTTTGc                                                                        <  1:2184249/101‑1 (MQ=255)
                aaGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGACCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGc                                                                        >  2:1622747/1‑101 (MQ=255)
                      aTCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGcc                                                                  >  2:1103230/1‑101 (MQ=255)
                        cGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCt                                                                <  1:864307/101‑1 (MQ=255)
                         gATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTc                                                               <  1:2356313/101‑1 (MQ=255)
                              aaCCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTcc                                                          >  1:1959195/1‑101 (MQ=255)
                                 cGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTgg                                                       <  1:4404564/101‑1 (MQ=255)
                                 cGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTgg                                                       >  2:575619/1‑101 (MQ=255)
                                   gCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggc                                                     <  1:2138065/101‑1 (MQ=255)
                                   gCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggc                                                     <  1:164948/101‑1 (MQ=255)
                                   gCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggc                                                     >  1:2716563/1‑101 (MQ=255)
                                    tcttCCGATCTTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggc                                                     <  2:475436/98‑1 (MQ=255)
                                      gTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggcggc                                                  >  1:3680545/1‑101 (MQ=255)
                                       tCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCa                                                 <  2:4287426/101‑1 (MQ=255)
                                       tCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTGGCCGTGCCCTCGTTCCTGGGCGGCa                                                 <  2:4039173/101‑1 (MQ=255)
                                         cGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAg                                               <  2:2334538/101‑1 (MQ=255)
                                          gATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGa                                              >  1:1296360/1‑101 (MQ=255)
                                          gATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGa                                              <  1:3812510/101‑1 (MQ=255)
                                          gATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGa                                              <  1:2366788/101‑1 (MQ=255)
                                           aTC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGaa                                             >  1:3546776/1‑101 (MQ=255)
                                           aTC.TACAACGAGGAGAACCGCCCCGGTGACCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGTCCTCGTTCCTGGGGGGCAAGac                                             >  2:680287/1‑100 (MQ=255)
                                             c.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACt                                           >  2:3392193/1‑101 (MQ=255)
                                               tACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTg                                          <  1:2735327/101‑1 (MQ=255)
                                                   aCGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATg                                      <  1:3315912/101‑1 (MQ=255)
                                                    cGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGc                                     >  1:1875940/1‑101 (MQ=255)
                                                      aggagAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCg                                   <  2:1992027/101‑1 (MQ=255)
                                                      aggagAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAAATGGATGCCg                                   >  1:1836854/1‑101 (MQ=255)
                                                          gaACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATgg                               >  2:871115/1‑101 (MQ=255)
                                                           aaCCGCCCCGGTGCCCCCGATGCCGCGGCCGCAA.GCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCACTGGc                              >  2:1193158/1‑101 (MQ=255)
                                                             ccGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCt                            <  2:611955/101‑1 (MQ=255)
                                                             ccGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCt                            <  1:2312688/101‑1 (MQ=255)
                                                              cGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTa                           >  1:664148/1‑101 (MQ=255)
                                                              cGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTa                           <  2:1848860/101‑1 (MQ=255)
                                                                ccccGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACa                         <  2:3083414/101‑1 (MQ=255)
                                                                ccccGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACa                         <  2:3205307/101‑1 (MQ=255)
                                                                ccccGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACa                         <  1:1166406/101‑1 (MQ=255)
                                                                ccccGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACa                         >  1:2599239/1‑101 (MQ=255)
                                                                ccccGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACa                         <  1:3392446/101‑1 (MQ=255)
                                                                 nccGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAg                        <  1:3495373/100‑1 (MQ=255)
                                                                 cccGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAg                        >  2:2228811/1‑101 (MQ=255)
                                                                  ccGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGc                       >  2:2563000/1‑101 (MQ=255)
                                                                  ccGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGc                       <  1:1361261/101‑1 (MQ=255)
                                                                   cGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGcc                      <  2:1724369/101‑1 (MQ=255)
                                                                   cGGTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGcc                      >  2:3630236/1‑101 (MQ=255)
                                                                    ggTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCa                     >  2:1575899/1‑101 (MQ=255)
                                                                     gTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAg                    <  2:1381096/101‑1 (MQ=255)
                                                                     gTGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAg                    <  1:2254605/101‑1 (MQ=255)
                                                                      tGCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGa                   <  1:575667/101‑1 (MQ=255)
                                                                       gCCCCCGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGAc                  >  1:3114686/1‑101 (MQ=255)
                                                                        cccccGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGAcc                 <  2:3672578/101‑1 (MQ=255)
                                                                        cccccGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGAcc                 <  1:3557032/101‑1 (MQ=255)
                                                                        cccccGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGAcc                 >  1:2872366/1‑101 (MQ=255)
                                                                        cccccGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGAcc                 <  1:1327164/101‑1 (MQ=255)
                                                                        cccccGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGAcc                 <  2:1949116/101‑1 (MQ=255)
                                                                         ccccGATGCCGCGGCCG.AAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGAccc                >  2:3577256/1‑100 (MQ=255)
                                                                         ccccGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCa                >  1:4136177/1‑101 (MQ=255)
                                                                          cccGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGATCTGGATGCCGATGGCCTACAGCCAGACCAc               <  2:984483/101‑1 (MQ=255)
                                                                           ccGATGCCGCGGCCG.AAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACg              >  2:3233351/1‑101 (MQ=255)
                                                                           ccGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACg              <  2:812314/101‑1 (MQ=255)
                                                                            cGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgg             >  2:2825263/1‑101 (MQ=255)
                                                                            cGATGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgg             >  2:133987/1‑101 (MQ=255)
                                                                              cgcagacgGCCG.AAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgggc           >  2:751965/7‑100 (MQ=37)
                                                                              aTGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgggc           <  1:602112/101‑2 (MQ=255)
                                                                               tGCCGCGGCCG.AAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgggcc          >  1:2674179/1‑99 (MQ=255)
                                                                               tGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgggcc          >  2:2963499/1‑99 (MQ=255)
                                                                               tGCCGCGGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgggcc          <  2:2914334/101‑3 (MQ=255)
                                                                                   gcgGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtctt      <  1:4124245/101‑1 (MQ=255)
                                                                                    gggCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtcttc     <  1:4612915/100‑1 (MQ=38)
                                                                                    cgGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtcttc     >  1:2492465/1‑101 (MQ=38)
                                                                                    cgGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtcttc     >  1:2411577/1‑101 (MQ=38)
                                                                                    cgGCCG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtcttc     <  1:647735/101‑1 (MQ=38)
                                                                                       ccG.AA.GGCGGCAAGGGCCAGCAGATCTTTTCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCTCTTCTAt  >  1:4051038/1‑101 (MQ=37)
                                                                                       ccG.AA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCTCTTCTAt  <  2:2795865/101‑1 (MQ=38)
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GAACATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATC.TACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCG.AA.GCGGCCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGGCTCTTCTAT  >  NC_014034/1794209‑1794395

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG