Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,813,081 T→G 100% intergenic (+125/‑93) glnA3 → / → fabD glutamine synthetase/ACP S‑malonyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,813,0810T→G100.0% 276.0 80intergenic (+125/‑93)glnA3/fabDglutamine synthetase/ACP S‑malonyltransferase
Reads supporting (aligned to +/- strand):  new base (63/17):  ref base (0/0):  total (63/17)

CTTTGGGCGCCCTTTTCTTTTGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCTCGGGGGTCCGGCGGCGCGAA.CGCCCGGCGGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAG  >  NC_014034/1812984‑1813178
                                                                                                  |                                                                                                  
cTTTGGGCGCCCTTTTCTTTTGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCgg                                                                                                 >  1:1310710/1‑101 (MQ=255)
cTTTGGGCGCCCTTTTCTTTTGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCgg                                                                                                 <  2:3788756/101‑1 (MQ=255)
  tttggCGCGCTTTTCTTTTGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCgggg                                                                                               >  2:120668/4‑101 (MQ=255)
   tGGGCGCCCTTTTCTTTTGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCggggg                                                                                              <  2:4102184/101‑1 (MQ=255)
   tGGGCGCCCTTTTCTTTTGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCggggg                                                                                              <  1:3282086/101‑1 (MQ=255)
     ggCGCCCTTTTCTTTTGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCTGGGGTc                                                                                            >  1:4463752/1‑101 (MQ=255)
      gcgcCCTTTTCTTTTGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTcc                                                                                           <  1:1185575/101‑1 (MQ=255)
       cgcCCTTTTCTTTTGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCcg                                                                                          <  1:123365/101‑1 (MQ=255)
       cgcCCTTTTCTTTTGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCcg                                                                                          <  1:4619821/101‑1 (MQ=255)
        gcCCTTTTCTTTTGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCcgg                                                                                         <  2:3469019/101‑1 (MQ=255)
        gcCCTTTTCTTTTGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCcgg                                                                                         <  1:1787992/101‑1 (MQ=255)
         cccTTTTCTTTTGCCGGGGTGCGACTGCGACTCTTTGCTTATTTGAGCCAAGAAAAAACCCGGCTCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCcggg                                                                                        <  1:3749930/101‑2 (MQ=255)
             tttcttttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGggggcg                                                                                    <  1:2505507/101‑1 (MQ=255)
             tttcttttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGggggcg                                                                                    >  1:1899105/1‑101 (MQ=255)
             tttcttttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGggggcg                                                                                    <  2:3165726/101‑1 (MQ=255)
               tcttttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGcgcg                                                                                  <  1:1342469/101‑1 (MQ=255)
                 ttttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaa                                                                                <  1:718524/101‑1 (MQ=255)
                 ttttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaa                                                                                <  1:1119402/101‑1 (MQ=255)
                  tttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaag                                                                               <  2:3487223/101‑2 (MQ=255)
                  tttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaag                                                                               >  1:3563765/1‑100 (MQ=255)
                  tttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaag                                                                               >  2:4667374/1‑100 (MQ=255)
                   ttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaagc                                                                              <  1:3480193/101‑3 (MQ=255)
                       gggggTGCGACTGCGACTCTTTGCGTATTTGAGCAAAAAAAAACCCCGGCGTTTTTTCTTGGTT.AAAATACGCCGGGGGGGTCCGGGGGCGCGAA.CCcccc                                                                         <  2:2821557/100‑2 (MQ=25)
                       cGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaagccccc                                                                          >  2:2212511/1‑95 (MQ=38)
                       cGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaagccccc                                                                          >  1:1189089/1‑95 (MQ=38)
                       cGGGGTCCGACTGCGACTCTTGGCGTATTTGAGCCAAGAAAAACCCCGGCGTTTTTTCTTGGTT.AAAATCCGCCGGGGGGGTCGGGGGGCGCGAA.CCcccc                                                                         <  2:2876162/101‑2 (MQ=25)
                        ggggTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaagcccccg                                                                         >  2:2409550/1‑94 (MQ=38)
                            tgcgactgcgacTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgc                                                                     >  2:3627552/1‑100 (MQ=37)
                               gactgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgcttc                                                                  >  1:4398759/1‑97 (MQ=25)
                               gactgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgcttc                                                                  >  2:4129333/1‑97 (MQ=25)
                               gactgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgcttc                                                                  >  1:1336451/1‑97 (MQ=25)
                                actgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTcc                                                                >  2:564065/1‑101 (MQ=25)
                                  tgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCgg                                                              >  2:4214237/1‑101 (MQ=25)
                                  tgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCgg                                                              >  1:1144240/1‑101 (MQ=25)
                                  tgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCgg                                                              >  2:801685/1‑101 (MQ=25)
                                       ctttttGCGTATTTGAGCCAAAAAAAACCCCGGCGTTTTTTCTTGGTT.CAAATCCGCCGGGGGGGTCGGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCt                                                         <  2:2988887/98‑1 (MQ=16)
                                       ctctTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCt                                                         >  1:1933250/1‑101 (MQ=25)
                                        tctTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTc                                                        >  2:3747225/1‑101 (MQ=25)
                                         ctTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTcc                                                       >  1:1502206/1‑101 (MQ=25)
                                          tttGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCg                                                      >  2:3086521/1‑101 (MQ=25)
                                             gCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGAc                                                   >  2:838749/1‑101 (MQ=25)
                                                tATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCt                                                >  2:192103/1‑101 (MQ=25)
                                                tATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCt                                                >  2:2486626/1‑101 (MQ=25)
                                                 aTTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTg                                               >  2:915709/1‑101 (MQ=25)
                                                  tttGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAA.GCCCCCGGAGCTTCCGGTTGCTCCGGACGCTGc                                              >  2:4663471/1‑101 (MQ=37)
                                                  tttGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGGCAGGGGGCGCCAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGc                                              >  2:3647550/1‑101 (MQ=25)
                                                    tGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGt                                            >  1:173575/1‑101 (MQ=25)
                                                     gAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTc                                           >  2:2315396/1‑101 (MQ=37)
                                                      aGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCa                                          >  1:4393769/1‑101 (MQ=25)
                                                      aGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCa                                          >  1:1162640/1‑101 (MQ=25)
                                                       gCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCaa                                         >  1:835443/1‑101 (MQ=37)
                                                         cAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGt                                       >  2:250587/1‑101 (MQ=37)
                                                          aaGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGtt                                      >  2:1954501/1‑101 (MQ=25)
                                                           aGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTa                                     >  2:1973219/1‑101 (MQ=25)
                                                              aaaacCCCGGGGTTTTTTTTTGGTTAAAAACCCGCGGGGGGGGCCGGGGGGCGAGAC.CCCCCGGGCGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTaga                                   <  1:2078877/101‑1 (MQ=255)
                                                               aaaaCCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAgaga                                 >  1:17878/1‑101 (MQ=25)
                                                                aaaCCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGaa                                >  1:3557810/1‑101 (MQ=25)
                                                                   cccGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGc                             >  2:4389417/1‑101 (MQ=25)
                                                                   cccGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGc                             >  1:3338108/1‑101 (MQ=25)
                                                                     cggctttttttCTTGGTT.CAAATCCGCCGGGGGGGTCGGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCa                           <  2:3792142/95‑1 (MQ=21)
                                                                     cGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCa                           >  2:1598908/1‑101 (MQ=25)
                                                                      ggCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAg                          >  1:824874/1‑101 (MQ=25)
                                                                          cTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGAttt                      >  1:632617/1‑101 (MQ=25)
                                                                             tttCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTc                   >  1:1592523/1‑101 (MQ=25)
                                                                              ttCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCa                  <  2:1502097/101‑1 (MQ=25)
                                                                               tCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCaa                 >  2:622905/1‑101 (MQ=25)
                                                                               tCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCaa                 >  2:2160954/1‑101 (MQ=25)
                                                                                cTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAag                >  1:2921366/1‑101 (MQ=25)
                                                                                cTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAag                >  2:4121127/1‑101 (MQ=25)
                                                                                 ttGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAagg               >  2:976548/1‑101 (MQ=25)
                                                                                  tGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAagga              >  2:3936702/1‑101 (MQ=25)
                                                                                  tGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAagaa              >  2:4313579/1‑99 (MQ=25)
                                                                                     tt.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGc           >  2:4332921/1‑101 (MQ=25)
                                                                                      t.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCg          >  1:4117785/1‑101 (MQ=25)
                                                                                        cAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCgg         >  2:1616873/1‑101 (MQ=25)
                                                                                        cAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCgg         >  2:3714787/1‑101 (MQ=25)
                                                                                          aaTACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGc       >  2:2777908/1‑101 (MQ=37)
                                                                                           aTACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCa      >  2:3059868/1‑101 (MQ=37)
                                                                                           aTACGCCGCGGGGGCCCGGGGGGGCGAAGCCCCCGGC.CCTTCCGGTTGCCGCGGACGCCGCGGCAAGTTAGAGAAGGCCCGATTCGCCAAGGCGGCgggga      >  2:4162112/1‑99 (MQ=255)
                                                                                            tACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCAt     >  2:2337833/1‑101 (MQ=37)
                                                                                             aCGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATg    >  2:4324890/1‑101 (MQ=37)
                                                                                             aCGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATg    >  2:2441444/1‑101 (MQ=37)
                                                                                              cGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGa   >  2:4362670/1‑101 (MQ=37)
                                                                                               gCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGNCNNGNg  >  1:789149/1‑101 (MQ=25)
                                                                                               gCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAg  >  2:4143351/1‑101 (MQ=37)
                                                                                               gCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAg  >  1:1724759/1‑101 (MQ=37)
                                                                                                  |                                                                                                  
CTTTGGGCGCCCTTTTCTTTTGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCTCGGGGGTCCGGCGGCGCGAA.CGCCCGGCGGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAG  >  NC_014034/1812984‑1813178

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG