Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,813,093	C→G	100%	intergenic (+137/‑81)	glnA3 → / → fabD	glutamine synthetase/ACP S‑malonyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,813,093	0	C→G	100.0%	252.4	75	intergenic (+137/‑81)	glnA3/fabD	glutamine synthetase/ACP S‑malonyltransferase
Reads supporting (aligned to +/- strand):  new base (67/8):  ref base (0/0):  total (67/8)

TTTCTTTTGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCTCGGGGGTCCGGCGGCGCGAA.CGCCCGGCGGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGT  >  NC_014034/1812997‑1813190                                                                                                  |                                                                                                   tttcttttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGggggcg                                                                                                <  2:3165726/101‑1 (MQ=255) tttcttttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGggggcg                                                                                                <  1:2505507/101‑1 (MQ=255) tttcttttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGggggcg                                                                                                >  1:1899105/1‑101 (MQ=255)   tcttttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGcgcg                                                                                              <  1:1342469/101‑1 (MQ=255)     ttttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaa                                                                                            <  1:718524/101‑1 (MQ=255)     ttttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaa                                                                                            <  1:1119402/101‑1 (MQ=255)      tttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaag                                                                                           <  2:3487223/101‑2 (MQ=255)      tttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaag                                                                                           >  1:3563765/1‑100 (MQ=255)      tttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaag                                                                                           >  2:4667374/1‑100 (MQ=255)       ttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaagc                                                                                          <  1:3480193/101‑3 (MQ=255)           gggggTGCGACTGCGACTCTTTGCGTATTTGAGCAAAAAAAAACCCCGGCGTTTTTTCTTGGTT.AAAATACGCCGGGGGGGTCCGGGGGCGCGAA.CCcccc                                                                                     <  2:2821557/100‑2 (MQ=25)           cGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaagccccc                                                                                      >  1:1189089/1‑95 (MQ=38)           cGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaagccccc                                                                                      >  2:2212511/1‑95 (MQ=38)           cGGGGTCCGACTGCGACTCTTGGCGTATTTGAGCCAAGAAAAACCCCGGCGTTTTTTCTTGGTT.AAAATCCGCCGGGGGGGTCGGGGGGCGCGAA.CCcccc                                                                                     <  2:2876162/101‑2 (MQ=25)            ggggTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaagcccccg                                                                                     >  2:2409550/1‑94 (MQ=38)                tgcgactgcgacTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgc                                                                                 >  2:3627552/1‑100 (MQ=37)                   gactgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgcttc                                                                              >  1:4398759/1‑97 (MQ=25)                   gactgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgcttc                                                                              >  2:4129333/1‑97 (MQ=25)                   gactgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgcttc                                                                              >  1:1336451/1‑97 (MQ=25)                    actgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTcc                                                                            >  2:564065/1‑101 (MQ=25)                      tgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCgg                                                                          >  1:1144240/1‑101 (MQ=25)                      tgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCgg                                                                          >  2:4214237/1‑101 (MQ=25)                      tgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCgg                                                                          >  2:801685/1‑101 (MQ=25)                           ctttttGCGTATTTGAGCCAAAAAAAACCCCGGCGTTTTTTCTTGGTT.CAAATCCGCCGGGGGGGTCGGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCt                                                                     <  2:2988887/98‑1 (MQ=16)                           ctctTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCt                                                                     >  1:1933250/1‑101 (MQ=25)                            tctTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTc                                                                    >  2:3747225/1‑101 (MQ=25)                             ctTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTcc                                                                   >  1:1502206/1‑101 (MQ=25)                              tttGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCg                                                                  >  2:3086521/1‑101 (MQ=25)                                 gCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGAc                                                               >  2:838749/1‑101 (MQ=25)                                    tATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCt                                                            >  2:2486626/1‑101 (MQ=25)                                    tATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCt                                                            >  2:192103/1‑101 (MQ=25)                                     aTTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTg                                                           >  2:915709/1‑101 (MQ=25)                                      tttGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAA.GCCCCCGGAGCTTCCGGTTGCTCCGGACGCTGc                                                          >  2:4663471/1‑101 (MQ=37)                                      tttGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGGCAGGGGGCGCCAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGc                                                          >  2:3647550/1‑101 (MQ=25)                                        tGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGt                                                        >  1:173575/1‑101 (MQ=25)                                         gAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTc                                                       >  2:2315396/1‑101 (MQ=37)                                          aGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCa                                                      >  1:4393769/1‑101 (MQ=25)                                          aGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCa                                                      >  1:1162640/1‑101 (MQ=25)                                           gCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCaa                                                     >  1:835443/1‑101 (MQ=37)                                             cAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGt                                                   >  2:250587/1‑101 (MQ=37)                                              aaGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGtt                                                  >  2:1954501/1‑101 (MQ=25)                                               aGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTa                                                 >  2:1973219/1‑101 (MQ=25)                                                  aaaacCCCGGGGTTTTTTTTTGGTTAAAAACCCGCGGGGGGGGCCGGGGGGCGAGAC.CCCCCGGGCGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTaga                                               <  1:2078877/101‑1 (MQ=255)                                                   aaaaCCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAgaga                                             >  1:17878/1‑101 (MQ=25)                                                    aaaCCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGaa                                            >  1:3557810/1‑101 (MQ=25)                                                       cccGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGc                                         >  1:3338108/1‑101 (MQ=25)                                                       cccGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGc                                         >  2:4389417/1‑101 (MQ=25)                                                         cggctttttttCTTGGTT.CAAATCCGCCGGGGGGGTCGGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCa                                       <  2:3792142/95‑1 (MQ=21)                                                         cGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCa                                       >  2:1598908/1‑101 (MQ=25)                                                          ggCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAg                                      >  1:824874/1‑101 (MQ=25)                                                              cTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGAttt                                  >  1:632617/1‑101 (MQ=25)                                                                 tttCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTc                               >  1:1592523/1‑101 (MQ=25)                                                                  ttCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCa                              <  2:1502097/101‑1 (MQ=25)                                                                   tCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCaa                             >  2:622905/1‑101 (MQ=25)                                                                   tCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCaa                             >  2:2160954/1‑101 (MQ=25)                                                                    cTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAag                            >  1:2921366/1‑101 (MQ=25)                                                                    cTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAag                            >  2:4121127/1‑101 (MQ=25)                                                                     ttGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAagg                           >  2:976548/1‑101 (MQ=25)                                                                      tGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAagga                          >  2:3936702/1‑101 (MQ=25)                                                                      tGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAagaa                          >  2:4313579/1‑99 (MQ=25)                                                                         tt.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGc                       >  2:4332921/1‑101 (MQ=25)                                                                          t.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCg                      >  1:4117785/1‑101 (MQ=25)                                                                            cAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCgg                     >  2:1616873/1‑101 (MQ=25)                                                                            cAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCgg                     >  2:3714787/1‑101 (MQ=25)                                                                              aaTACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGc                   >  2:2777908/1‑101 (MQ=37)                                                                               aTACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCa                  >  2:3059868/1‑101 (MQ=37)                                                                               aTACGCCGCGGGGGCCCGGGGGGGCGAAGCCCCCGGC.CCTTCCGGTTGCCGCGGACGCCGCGGCAAGTTAGAGAAGGCCCGATTCGCCAAGGCGGCgggga                  >  2:4162112/1‑99 (MQ=255)                                                                                tACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCAt                 >  2:2337833/1‑101 (MQ=37)                                                                                 aCGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATg                >  2:2441444/1‑101 (MQ=37)                                                                                 aCGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATg                >  2:4324890/1‑101 (MQ=37)                                                                                  cGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGa               >  2:4362670/1‑101 (MQ=37)                                                                                   gCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGNCNNGNg              >  1:789149/1‑101 (MQ=25)                                                                                   gCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAg              >  2:4143351/1‑101 (MQ=37)                                                                                   gCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAg              >  1:1724759/1‑101 (MQ=37)                                                                                     cgcGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCg            >  1:2156774/3‑101 (MQ=37)                                                                                       cGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTg          >  1:3756273/1‑101 (MQ=38)                                                                                        gggggTCTGGGGGCGCGAA.GCCCCCGGGGCTTCCGGTTGCTCCGGACGCTGCGTCAAGCTTGAGAAGGCCAGATTTCTCAAGGGGGCGGGCATGAGCGTgg         >  2:1219808/1‑101 (MQ=255)                                                                                        gggggTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTgg         >  2:4172337/1‑101 (MQ=38)                                                                                        gggggTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTgg         >  1:539481/1‑101 (MQ=38)                                                                                        gggggTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTgg         >  2:1541399/1‑101 (MQ=38)                                                                                            gTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATg     >  1:2251609/1‑101 (MQ=38)                                                                                            gTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATg     >  2:795242/1‑101 (MQ=38)                                                                                             tCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATgg    >  2:3252704/1‑101 (MQ=255)                                                                                               cgggggCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCCTGGgt  >  2:2438943/1‑101 (MQ=255)                                                                                                  |                                                                                                   TTTCTTTTGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCTCGGGGGTCCGGCGGCGCGAA.CGCCCGGCGGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGT  >  NC_014034/1812997‑1813190 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG