Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,813,103 G→C 100% intergenic (+147/‑71) glnA3 → / → fabD glutamine synthetase/ACP S‑malonyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,813,1030G→C100.0% 238.5 72intergenic (+147/‑71)glnA3/fabDglutamine synthetase/ACP S‑malonyltransferase
Reads supporting (aligned to +/- strand):  new base (66/6):  ref base (0/0):  total (66/6)

CGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCTCGGGGGTCCGGCGGCGCGAA.CGCCCGGCGGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTT  >  NC_014034/1813007‑1813193
                                                                                                  |                                                                                          
gggggTGCGACTGCGACTCTTTGCGTATTTGAGCAAAAAAAAACCCCGGCGTTTTTTCTTGGTT.AAAATACGCCGGGGGGGTCCGGGGGCGCGAA.CCcccc                                                                                        <  2:2821557/100‑2 (MQ=25)
cGGGGTCCGACTGCGACTCTTGGCGTATTTGAGCCAAGAAAAACCCCGGCGTTTTTTCTTGGTT.AAAATCCGCCGGGGGGGTCGGGGGGCGCGAA.CCcccc                                                                                        <  2:2876162/101‑2 (MQ=25)
     tgcgactgcgacTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgc                                                                                    >  2:3627552/1‑100 (MQ=37)
        gactgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgcttc                                                                                 >  1:1336451/1‑97 (MQ=25)
        gactgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgcttc                                                                                 >  2:4129333/1‑97 (MQ=25)
        gactgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgcttc                                                                                 >  1:4398759/1‑97 (MQ=25)
         actgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTcc                                                                               >  2:564065/1‑101 (MQ=25)
           tgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCgg                                                                             >  2:4214237/1‑101 (MQ=25)
           tgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCgg                                                                             >  2:801685/1‑101 (MQ=25)
           tgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCgg                                                                             >  1:1144240/1‑101 (MQ=25)
                ctttttGCGTATTTGAGCCAAAAAAAACCCCGGCGTTTTTTCTTGGTT.CAAATCCGCCGGGGGGGTCGGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCt                                                                        <  2:2988887/98‑1 (MQ=16)
                ctctTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCt                                                                        >  1:1933250/1‑101 (MQ=25)
                 tctTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTc                                                                       >  2:3747225/1‑101 (MQ=25)
                  ctTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTcc                                                                      >  1:1502206/1‑101 (MQ=25)
                   tttGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCg                                                                     >  2:3086521/1‑101 (MQ=25)
                      gCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGAc                                                                  >  2:838749/1‑101 (MQ=25)
                         tATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCt                                                               >  2:2486626/1‑101 (MQ=25)
                         tATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCt                                                               >  2:192103/1‑101 (MQ=25)
                          aTTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTg                                                              >  2:915709/1‑101 (MQ=25)
                           tttGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAA.GCCCCCGGAGCTTCCGGTTGCTCCGGACGCTGc                                                             >  2:4663471/1‑101 (MQ=37)
                           tttGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGGCAGGGGGCGCCAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGc                                                             >  2:3647550/1‑101 (MQ=25)
                             tGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGt                                                           >  1:173575/1‑101 (MQ=25)
                              gAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTc                                                          >  2:2315396/1‑101 (MQ=37)
                               aGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCa                                                         >  1:1162640/1‑101 (MQ=25)
                               aGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCa                                                         >  1:4393769/1‑101 (MQ=25)
                                gCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCaa                                                        >  1:835443/1‑101 (MQ=37)
                                  cAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGt                                                      >  2:250587/1‑101 (MQ=37)
                                   aaGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGtt                                                     >  2:1954501/1‑101 (MQ=25)
                                    aGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTa                                                    >  2:1973219/1‑101 (MQ=25)
                                       aaaacCCCGGGGTTTTTTTTTGGTTAAAAACCCGCGGGGGGGGCCGGGGGGCGAGAC.CCCCCGGGCGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTaga                                                  <  1:2078877/101‑1 (MQ=255)
                                        aaaaCCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAgaga                                                >  1:17878/1‑101 (MQ=25)
                                         aaaCCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGaa                                               >  1:3557810/1‑101 (MQ=25)
                                            cccGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGc                                            >  2:4389417/1‑101 (MQ=25)
                                            cccGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGc                                            >  1:3338108/1‑101 (MQ=25)
                                              cggctttttttCTTGGTT.CAAATCCGCCGGGGGGGTCGGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCa                                          <  2:3792142/95‑1 (MQ=21)
                                              cGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCa                                          >  2:1598908/1‑101 (MQ=25)
                                               ggCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAg                                         >  1:824874/1‑101 (MQ=25)
                                                   cTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGAttt                                     >  1:632617/1‑101 (MQ=25)
                                                      tttCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTc                                  >  1:1592523/1‑101 (MQ=25)
                                                       ttCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCa                                 <  2:1502097/101‑1 (MQ=25)
                                                        tCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCaa                                >  2:2160954/1‑101 (MQ=25)
                                                        tCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCaa                                >  2:622905/1‑101 (MQ=25)
                                                         cTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAag                               >  2:4121127/1‑101 (MQ=25)
                                                         cTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAag                               >  1:2921366/1‑101 (MQ=25)
                                                          ttGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAagg                              >  2:976548/1‑101 (MQ=25)
                                                           tGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAagga                             >  2:3936702/1‑101 (MQ=25)
                                                           tGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAagaa                             >  2:4313579/1‑99 (MQ=25)
                                                              tt.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGc                          >  2:4332921/1‑101 (MQ=25)
                                                               t.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCg                         >  1:4117785/1‑101 (MQ=25)
                                                                 cAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCgg                        >  2:3714787/1‑101 (MQ=25)
                                                                 cAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCgg                        >  2:1616873/1‑101 (MQ=25)
                                                                   aaTACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGc                      >  2:2777908/1‑101 (MQ=37)
                                                                    aTACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCa                     >  2:3059868/1‑101 (MQ=37)
                                                                    aTACGCCGCGGGGGCCCGGGGGGGCGAAGCCCCCGGC.CCTTCCGGTTGCCGCGGACGCCGCGGCAAGTTAGAGAAGGCCCGATTCGCCAAGGCGGCgggga                     >  2:4162112/1‑99 (MQ=255)
                                                                     tACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCAt                    >  2:2337833/1‑101 (MQ=37)
                                                                      aCGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATg                   >  2:2441444/1‑101 (MQ=37)
                                                                      aCGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATg                   >  2:4324890/1‑101 (MQ=37)
                                                                       cGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGa                  >  2:4362670/1‑101 (MQ=37)
                                                                        gCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGNCNNGNg                 >  1:789149/1‑101 (MQ=25)
                                                                        gCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAg                 >  1:1724759/1‑101 (MQ=37)
                                                                        gCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAg                 >  2:4143351/1‑101 (MQ=37)
                                                                          cgcGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCg               >  1:2156774/3‑101 (MQ=37)
                                                                            cGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTg             >  1:3756273/1‑101 (MQ=38)
                                                                             gggggTCTGGGGGCGCGAA.GCCCCCGGGGCTTCCGGTTGCTCCGGACGCTGCGTCAAGCTTGAGAAGGCCAGATTTCTCAAGGGGGCGGGCATGAGCGTgg            >  2:1219808/1‑101 (MQ=255)
                                                                             gggggTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTgg            >  1:539481/1‑101 (MQ=38)
                                                                             gggggTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTgg            >  2:4172337/1‑101 (MQ=38)
                                                                             gggggTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTgg            >  2:1541399/1‑101 (MQ=38)
                                                                                 gTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATg        >  2:795242/1‑101 (MQ=38)
                                                                                 gTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATg        >  1:2251609/1‑101 (MQ=38)
                                                                                  tCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATgg       >  2:3252704/1‑101 (MQ=255)
                                                                                    cgggggCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCCTGGgt     >  2:2438943/1‑101 (MQ=255)
                                                                                     gggggcgCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGgtg    >  1:4261984/4‑101 (MQ=255)
                                                                                     gggggcgCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGgtg    >  1:4232699/4‑101 (MQ=255)
                                                                                       gggcgCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGTATGGGTGtt  >  1:2395688/2‑101 (MQ=255)
                                                                                                  |                                                                                          
CGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCTCGGGGGTCCGGCGGCGCGAA.CGCCCGGCGGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTT  >  NC_014034/1813007‑1813193

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG