Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,813,109	C→G	5.1%	intergenic (+153/‑65)	glnA3 → / → fabD	glutamine synthetase/ACP S‑malonyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,813,109	0	C→G	5.1%	2.6 ‑3.8 ‑0.0	87	intergenic (+153/‑65)	glnA3/fabD	glutamine synthetase/ACP S‑malonyltransferase
Reads supporting (aligned to +/- strand):  new base (2/3):  ref base (72/10):  total (74/13)
Fisher's exact test strand distribution p-value = 2.24e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.84e-01

TGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCTCGGGGGTCCGGCGGCGCGAA.CGCCCGGCGGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTCCCCGGTCAGGG  >  NC_014034/1813012‑1813205                                                                                                    |                                                                                                 tgcgactgcgacTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgc                                                                                                >  2:3627552/1‑100 (MQ=37)    gactgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgcttc                                                                                             >  1:1336451/1‑97 (MQ=25)    gactgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgcttc                                                                                             >  1:4398759/1‑97 (MQ=25)    gactgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgcttc                                                                                             >  2:4129333/1‑97 (MQ=25)     actgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTcc                                                                                           >  2:564065/1‑101 (MQ=25)       tgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCgg                                                                                         >  1:1144240/1‑101 (MQ=25)       tgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCgg                                                                                         >  2:801685/1‑101 (MQ=25)       tgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCgg                                                                                         >  2:4214237/1‑101 (MQ=25)            ctttttGCGTATTTGAGCCAAAAAAAACCCCGGCGTTTTTTCTTGGTT.CAAATCCGCCGGGGGGGTCGGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCt                                                                                    <  2:2988887/98‑1 (MQ=16)            ctctTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCt                                                                                    >  1:1933250/1‑101 (MQ=25)             tctTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTc                                                                                   >  2:3747225/1‑101 (MQ=25)              ctTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTcc                                                                                  >  1:1502206/1‑101 (MQ=25)               tttGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCg                                                                                 >  2:3086521/1‑101 (MQ=25)                  gCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGAc                                                                              >  2:838749/1‑101 (MQ=25)                     tATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCt                                                                           >  2:192103/1‑101 (MQ=25)                     tATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCt                                                                           >  2:2486626/1‑101 (MQ=25)                      aTTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTg                                                                          >  2:915709/1‑101 (MQ=25)                       tttGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAA.GCCCCCGGAGCTTCCGGTTGCTCCGGACGCTGc                                                                         >  2:4663471/1‑101 (MQ=37)                       tttGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGGCAGGGGGCGCCAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGc                                                                         >  2:3647550/1‑101 (MQ=25)                         tGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGt                                                                       >  1:173575/1‑101 (MQ=25)                          gAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTc                                                                      >  2:2315396/1‑101 (MQ=37)                           aGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCa                                                                     >  1:1162640/1‑101 (MQ=25)                           aGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCa                                                                     >  1:4393769/1‑101 (MQ=25)                            gCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCaa                                                                    >  1:835443/1‑101 (MQ=37)                              cAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGt                                                                  >  2:250587/1‑101 (MQ=37)                               aaGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGtt                                                                 >  2:1954501/1‑101 (MQ=25)                                aGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTa                                                                >  2:1973219/1‑101 (MQ=25)                                   aaaacCCCGGGGTTTTTTTTTGGTTAAAAACCCGCGGGGGGGGCCGGGGGGCGAGAC.CCCCCGGGCGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTaga                                                              <  1:2078877/101‑1 (MQ=255)                                    aaaaCCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAgaga                                                            >  1:17878/1‑101 (MQ=25)                                     aaaCCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGaa                                                           >  1:3557810/1‑101 (MQ=25)                                        cccGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGc                                                        >  1:3338108/1‑101 (MQ=25)                                        cccGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGc                                                        >  2:4389417/1‑101 (MQ=25)                                          cggctttttttCTTGGTT.CAAATCCGCCGGGGGGGTCGGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCa                                                      <  2:3792142/95‑1 (MQ=21)                                          cGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCa                                                      >  2:1598908/1‑101 (MQ=25)                                           ggCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAg                                                     >  1:824874/1‑101 (MQ=25)                                               cTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGAttt                                                 >  1:632617/1‑101 (MQ=25)                                                  tttCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTc                                              >  1:1592523/1‑101 (MQ=25)                                                   ttCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCa                                             <  2:1502097/101‑1 (MQ=25)                                                    tCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCaa                                            >  2:622905/1‑101 (MQ=25)                                                    tCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCaa                                            >  2:2160954/1‑101 (MQ=25)                                                     cTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAag                                           >  2:4121127/1‑101 (MQ=25)                                                     cTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAag                                           >  1:2921366/1‑101 (MQ=25)                                                      ttGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAagg                                          >  2:976548/1‑101 (MQ=25)                                                       tGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAagga                                         >  2:3936702/1‑101 (MQ=25)                                                       tGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAagaa                                         >  2:4313579/1‑99 (MQ=25)                                                          tt.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGc                                      >  2:4332921/1‑101 (MQ=25)                                                           t.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCg                                     >  1:4117785/1‑101 (MQ=25)                                                             cAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCgg                                    >  2:3714787/1‑101 (MQ=25)                                                             cAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCgg                                    >  2:1616873/1‑101 (MQ=25)                                                               aaTACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGc                                  >  2:2777908/1‑101 (MQ=37)                                                                aTACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCa                                 >  2:3059868/1‑101 (MQ=37)                                                                aTACGCCGCGGGGGCCCGGGGGGGCGAAGCCCCCGGC.CCTTCCGGTTGCCGCGGACGCCGCGGCAAGTTAGAGAAGGCCCGATTCGCCAAGGCGGCgggga                                 >  2:4162112/1‑99 (MQ=255)                                                                 tACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCAt                                >  2:2337833/1‑101 (MQ=37)                                                                  aCGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATg                               >  2:2441444/1‑101 (MQ=37)                                                                  aCGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATg                               >  2:4324890/1‑101 (MQ=37)                                                                   cGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGa                              >  2:4362670/1‑101 (MQ=37)                                                                    gCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGNCNNGNg                             >  1:789149/1‑101 (MQ=25)                                                                    gCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAg                             >  2:4143351/1‑101 (MQ=37)                                                                    gCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAg                             >  1:1724759/1‑101 (MQ=37)                                                                      cgcGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCg                           >  1:2156774/3‑101 (MQ=37)                                                                        cGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTg                         >  1:3756273/1‑101 (MQ=38)                                                                         gggggTCTGGGGGCGCGAA.GCCCCCGGGGCTTCCGGTTGCTCCGGACGCTGCGTCAAGCTTGAGAAGGCCAGATTTCTCAAGGGGGCGGGCATGAGCGTgg                        >  2:1219808/1‑101 (MQ=255)                                                                         gggggTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTgg                        >  1:539481/1‑101 (MQ=38)                                                                         gggggTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTgg                        >  2:4172337/1‑101 (MQ=38)                                                                         gggggTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTgg                        >  2:1541399/1‑101 (MQ=38)                                                                             gTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATg                    >  2:795242/1‑101 (MQ=38)                                                                             gTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATg                    >  1:2251609/1‑101 (MQ=38)                                                                              tCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATgg                   >  2:3252704/1‑101 (MQ=255)                                                                                cgggggCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCCTGGgt                 >  2:2438943/1‑101 (MQ=255)                                                                                 gggggcgCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGgtg                >  1:4261984/4‑101 (MQ=255)                                                                                 gggggcgCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGgtg                >  1:4232699/4‑101 (MQ=255)                                                                                   gggcgCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGTATGGGTGtt              >  1:2395688/2‑101 (MQ=255)                                                                                      gggcgcccccccGGGCGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTcc            <  1:1102429/92‑1 (MQ=255)                                                                                      cgcgaagcccccGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTccc           >  2:922512/10‑101 (MQ=255)                                                                                       gcgacgcccccGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTcccc          <  2:2921137/93‑1 (MQ=255)                                                                                       gcgaagcccccGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGTATGGGTGTTcccc          <  2:2395526/93‑1 (MQ=255)                                                                                       gcgaagcccccGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTcccc          >  1:304705/9‑101 (MQ=255)                                                                                       gcgaagcccccGGC.GCTTCCGGGTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGGTTTGTCAAGGAGGCGGGCATGAGCGTGGCAAGGGGGTTccac          >  2:1669367/9‑99 (MQ=255)                                                                                        cgaagcccccGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTCCCCg         >  1:1635139/8‑101 (MQ=255)                                                                                         gaagcccccGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTCCCCgg        >  2:3285356/7‑101 (MQ=255)                                                                                          tagcccccGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTCCCCGGt       <  1:1656498/96‑1 (MQ=255)                                                                                          aagcccccGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTCCCCGGt       >  1:363485/6‑101 (MQ=255)                                                                                          aagcccccGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTCCCCGGt       >  2:639518/6‑101 (MQ=255)                                                                                           agcccccGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTCCCCGGta      >  2:1656387/5‑100 (MQ=255)                                                                                           agcccccGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTCCCCGGTc      <  2:3755998/97‑1 (MQ=255)                                                                                            gcccccGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTCCCCGGTCa     >  1:4159302/4‑101 (MQ=255)                                                                                            gcccccGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTCCCCGGTCa     >  2:1669633/4‑101 (MQ=255)                                                                                            ccccccGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTCCCCGGTCa     <  2:3014139/98‑1 (MQ=255)                                                                                            ccccccGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTCCCCGGTCa     <  2:1933115/98‑1 (MQ=255)                                                                                             cccccGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTCCCCGGTCAg    <  2:718310/99‑1 (MQ=255)                                                                                              ccccGGGCGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTCCCCGGTCAg    <  1:2757846/100‑1 (MQ=255)                                                                                               cccGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTCCCCGGTCAggg  >  2:1038244/1‑101 (MQ=255)                                                                                               cccGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTCCCCGGTCAggg  >  2:2438178/1‑101 (MQ=255)                                                                                               cccGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGAGCATGAGCGTGGCATGGGTGTTCCCCGGTCAggg  >  2:1370348/1‑101 (MQ=255)                                                                                                    |                                                                                                 TGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCTCGGGGGTCCGGCGGCGCGAA.CGCCCGGCGGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTCCCCGGTCAGGG  >  NC_014034/1813012‑1813205 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG