Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,965,271	C→G	100%	intergenic (+39/+53)	bdhA → / ← RCAP_rcc01818	3‑hydroxybutyrate dehydrogenase/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,965,271	0	C→G	100.0%	38.9	14	intergenic (+39/+53)	bdhA/RCAP_rcc01818	3‑hydroxybutyrate dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/8):  ref base (0/0):  total (6/8)

GGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGCGCCTCGGGGCAAGCCCGAGGCGACCCGCC..GCGGGCT.CCG.CC.CCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTC  >  NC_014034/1965178‑1965366                                                                                                |                                                                                                   ggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGGGCACCGCC...CGGGcgggc                                                                                            >  2:4244043/1‑97 (MQ=255)   cgcAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCGCC..GGgggggcgcc                                                                                           >  2:365681/1‑95 (MQ=38)         tCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCGCC..GGGGGGG.CCC.cc.ccccc                                                                                  >  1:1399186/1‑98 (MQ=37)             cGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAAGCGACCCGCC..GCGGGGG.CCC.cc.cccccgccc                                                                              >  2:4261370/1‑94 (MQ=255)                   gtcgttttGTGTGGGGGGCGGCGGGCCGGCGCTGTGAGAGTTTCCGCGGCGGGCCCACCCGGGGCCGACCCGCC..GGGGGCT.CCGCCC.CCGGACCCCCGAgg                                                                         <  2:3753602/96‑1 (MQ=9)                             gtgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCGCCGGGGGGGCC.CCC.CC.CCGGCCCCCCCGGGGAttttggg                                                                >  1:2204294/1‑97 (MQ=16)                                     cggcTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCGCC..GGGGGCT.CCG.CCACCGGACCCCCGAGGTATTTCAGGCAAGATGaa                                                       >  2:2817148/1‑101 (MQ=25)                                                     tgAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCGCC..GGGGGGT.CCGCCC.CCCGACCCCCCAGGGATTTTCGGGAAGATGAAAAAgggggggccaggg                                       >  2:2971960/1‑92 (MQ=12)                                                        gagaTTTCGCCTCGGGGCAAGCCCGAGGCGACCCGCC..GGGGGCT.CCGCCC.CCGGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGGGGCGCAggcggc                                    >  2:366990/1‑101 (MQ=21)                                                               cgccgggggcCACGCCCGGGGCCCCCCGCC..GGGGGCT.CCGCCC.CCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCttcttc                             <  1:36082/95‑1 (MQ=21)                                                                  gggggcgggggCCCGCGGCGCCCCGCC..GGGGGCT.CCGCCC.CCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTcgcc                          <  1:755212/91‑1 (MQ=21)                                                                  ctgggggCACGCCCGGGGCCCCCCGCC..GGGGGCT.CCGCCC.CCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTcgcc                          <  2:2592011/98‑1 (MQ=21)                                                                     ggggCAAGCCCGAGGCGACCCGCC..GGGGGCT.CCGCCC.CCCGACCCCCGAGGGATTTCAGGCAAAATGAAAGAAGGGCGCTCCGGCGGCCTTCTTCgccgcc                       >  2:1372588/1‑101 (MQ=21)                                                                              ccgcgcccccccGCC..GGGGGCT.CCGCCC.CCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGgt              <  2:1772453/92‑1 (MQ=25)                                                                                   cccccccGCC..GGGGGCT.CCGCCC.CCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGtctc         <  2:2198533/97‑1 (MQ=25)                                                                                       cccGCC..GGGGGCT.CCGCCC.CCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGc     <  1:367027/101‑1 (MQ=255)                                                                                        ccgcc..gGGGGCT.CCGCCC.CCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGct    <  2:2204144/101‑1 (MQ=255)                                                                                         cgcc..ggGGGCT.CCGCCC.CCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGctt   <  2:2331508/101‑1 (MQ=255)                                                                                         cgcc..ggGGGCT.CCGCCC.CCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGAGGCTCTCGGGCGGCCTTCTTCGCCGCCTTGCCCGTTGTCTTTGGctt   <  2:748431/101‑1 (MQ=21)                                                                                          gcc..ggGGGCTCCCC.CC.CCGGACCCCCGAGGGATTTTAGGGAAGATGAAAGAGGGGCGCTCAGGCGGCCCTCTTCGCCGCCTTGCCCGGGGTCTCGGGcttc  >  2:2796159/1‑101 (MQ=255)                                                                                          gcc..ggGGGCT.CCGCCC.CCGGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCCTGCCCGGCGTCTCGGGcttc  >  2:583462/1‑101 (MQ=37)                                                                                                |                                                                                                   GGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGCGCCTCGGGGCAAGCCCGAGGCGACCCGCC..GCGGGCT.CCG.CC.CCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTC  >  NC_014034/1965178‑1965366 Base quality scores:  ATCG  < 3 ≤  ATCG  < 8 ≤  ATCG  < 20 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG