Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,976,892 G→C 88.6% intergenic (‑71/+9) fba ← / ← gap1 fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,976,8920G→C88.6% 30.9 ‑0.6 ‑0.2143intergenic (‑71/+9)fba/gap1fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (92/34):  ref base (11/6):  total (103/40)
Fisher's exact test strand distribution p-value = 5.66e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.04e-01

Only 100 of 156 total aligned reads displayed.

TTGCCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCGGCG.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT  >  NC_014034/1976798‑1976990
                                                                                                       |                                                                                                   
        gggTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGAt                                                                                      >  2:238087/1‑101 (MQ=255)
         ggTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATc                                                                                     >  2:458765/1‑101 (MQ=255)
            gATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGc                                                                                  >  2:3474290/1‑101 (MQ=255)
            gATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGc                                                                                  >  2:2640615/1‑101 (MQ=255)
            gATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGc                                                                                  >  2:40721/1‑101 (MQ=255)
            gATCAGTCCCATTTTCTCATTTCCTTGAGTTCGCGGCTTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGCCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGc                                                                                  <  2:428671/101‑1 (MQ=25)
             ttcggTGCCTTTTTCTCATTTCTTTGGGTGCGC.GCCTTTTTCCTGG.CC.C.AAA.ATCGG.CGGGGGGCC.GGGGGCCAC.CC.CCCCCGGC.TTTTCTCAGATCAGCt                                                                                 <  2:1884690/97‑1 (MQ=255)
              tCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCtt                                                                                >  2:683268/1‑101 (MQ=255)
                aGTGCGATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGc                                                                              >  2:200832/1‑101 (MQ=37)
                 gTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGcc                                                                             >  1:1904731/1‑101 (MQ=255)
                  tgcttttttCTCTTTT.CTTGGGTTCGCGGCTTTTTTTGTGG.CC.C.AAA.ACCCG.GGGGGGGCG.GGGGG.CCG.CC.CCCCCCGGCTTTTCTCAGATCAGCTTGccc                                                                            <  1:1884390/96‑1 (MQ=255)
                  tgcttttttCTCTGTCCCTTGAG.TCGGCGCCTTTTTTTTGGCCC.A.AAA.TCCGC.GGGGGGCCG.GGGGG.CGG.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGccc                                                                            <  1:669645/96‑1 (MQ=255)
                  tGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGccc                                                                            <  2:2823605/101‑1 (MQ=255)
                    cctttttCTCAGTTCCTTGGGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TCCGC.CGGGGGTCG.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCAt                                                                          <  2:2677619/98‑1 (MQ=25)
                      aTTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCg                                                                        >  1:969745/1‑101 (MQ=255)
                      aTTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCg                                                                        >  2:751278/1‑101 (MQ=255)
                        tttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTGGG.CC.A.AAA.TCCGC.GGGGGGTCC.GGGGG.CAG.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGcc                                                                      <  1:2970811/101‑1 (MQ=25)
                          tcccAGTTCCTTGAGTCCGC.GCCTTTTTTTTGG.GC.GAAAA.TCCGC.GGGGGGTCG.GGGGG.CGG.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCAc                                                                    <  1:4473853/98‑1 (MQ=255)
                             cAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCAcggc                                                                 >  2:4173672/1‑101 (MQ=255)
                              aGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCAcggcg                                                                >  1:3703873/1‑101 (MQ=255)
                              aGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCACATCGCCAcggcg                                                                >  1:3108311/1‑101 (MQ=37)
                                 tctttGGGTTCGC.GCCTTTTTTTTGG.CCAA.AAA.CCCGC.GGGAGGCCC.GGGGG.CCC.CC.CCCCGGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa                                                             <  1:3426563/98‑1 (MQ=9)
                                 tCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa                                                             >  1:247143/1‑101 (MQ=255)
                                  ccTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTAt                                                            >  1:1723324/1‑101 (MQ=255)
                                   tttGTGTGCGCGG.CTTTTTTTTGG.CC...AAA.AACGCGCGGGGGGCCGGGGGG.CGG.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATc                                                           <  2:3367850/100‑1 (MQ=9)
                                   ctggggTTCGGGGCTTTTTTCTGGG.CC.G.AAA.TCCGC.GGGGGGTCG.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATc                                                           <  2:2561886/96‑1 (MQ=21)
                                   cTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATc                                                           >  2:2990/1‑101 (MQ=255)
                                   cTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATc                                                           >  1:78589/1‑101 (MQ=255)
                                   cTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATc                                                           >  1:3637101/1‑101 (MQ=255)
                                    ttggttccgcgG.CTTTTTTTTTG.CC.A.AAA.AACGG.CGGGGGGCC.GGGGG.CCGCCC.CCCCGGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCg                                                          <  2:1119267/94‑1 (MQ=12)
                                    ttGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCg                                                          >  1:1990047/1‑101 (MQ=255)
                                     tGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCgg                                                         >  1:3866042/1‑101 (MQ=255)
                                     tGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCgg                                                         >  1:2187997/1‑101 (MQ=255)
                                       aGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGcc                                                       >  1:49283/1‑101 (MQ=255)
                                        gTTCGCGGCCTTTTTCTTNG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CGC.CG.CCNCCGGC.TTTTCTCAGATCNNNNNGCCNCTCGNCANNTCCGCCTCGGCCa                                                      >  1:2847708/1‑101 (MQ=16)
                                           cgcgCCCTTTTCCTGGG.CG.A.AAA.CGCGC.GGGGGGCGC.GGGCG.CCC.CC.CCCCGGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGc                                                   <  1:4577346/101‑1 (MQ=12)
                                            gcgGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGca                                                  >  2:4577047/1‑100 (MQ=255)
                                            gcgGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCg                                                  >  2:1511136/1‑101 (MQ=255)
                                            gcgGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCg                                                  >  2:318033/1‑101 (MQ=255)
                                             ggccttttttCTGGG.CC.A.AAA.TCCGC.GGGGGGCCG.GGGGG.CGC.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCgg                                                 <  2:46105/96‑1 (MQ=16)
                                             cgGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCgg                                                 >  2:2035333/1‑101 (MQ=255)
                                               gtcTTTTTCTGGG.CC.G.AAA.TCCGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTAAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGtt                                               <  2:3794710/99‑1 (MQ=25)
                                               gcttttttCTTGT.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGtt                                               <  2:779238/98‑1 (MQ=37)
                                               gCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGtt                                               <  2:2233969/101‑1 (MQ=255)
                                               gCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGtt                                               >  2:4215460/1‑101 (MQ=255)
                                                ccTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTc                                              >  2:802401/1‑101 (MQ=255)
                                                 cTTTTTTTTGG.CC.A.AAA.ACCCG.CGGGGGGCG.GGGGGCCGG.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTc                                              <  1:4173960/101‑1 (MQ=12)
                                                  tttttCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCCTGCGGTTCga                                            >  2:2622863/1‑101 (MQ=37)
                                                  tttttCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCga                                            >  2:462207/1‑101 (MQ=255)
                                                  tttttCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGGTCga                                            >  2:3901438/1‑101 (MQ=37)
                                                  tttttCGTTG.CC.C.AAA.ATGGC.CGGGGGGCCGGGGGG.CCG.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCg                                             <  1:661447/101‑1 (MQ=12)
                                                   ttttCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgag                                           >  2:4440804/1‑101 (MQ=255)
                                                    tttCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgaga                                          >  1:915922/1‑101 (MQ=255)
                                                       cTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGc                                       >  1:3235853/1‑101 (MQ=255)
                                                         tGG.CC.G.AAA.TACGC.CGGGGGGCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCACTGCCACGGCGGTATCGGCCATGCGGTTCGGGAAGccc                                     >  2:3657035/1‑101 (MQ=25)
                                                          gg.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcccc                                    >  1:3297752/1‑101 (MQ=255)
                                                           g.CC.G.AAATTCCGC.GGGGGGTCG.GGGGG.CCC.CC.CCCCGGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcccc                                    <  1:4410992/101‑1 (MQ=18)
                                                             cc.G.AAA.T.CCC.CGGGGGGCC.GGGGG.CCGCCC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcat                                  <  2:3553793/101‑1 (MQ=17)
                                                              c.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcatt                                 >  2:1902401/1‑101 (MQ=255)
                                                              c.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcatt                                 >  1:1800480/1‑101 (MQ=255)
                                                              c.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcatt                                 >  2:1697242/1‑101 (MQ=255)
                                                                g.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattc                                >  2:3759191/1‑101 (MQ=255)
                                                                caaaa.TCCGC.GGGGGGCCG.GGGGG.CGC.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcatt                                 <  1:2838795/99‑1 (MQ=17)
                                                                   aa.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcat                              >  1:4513782/1‑101 (MQ=255)
                                                                    gaaatcggcGGGGGGCC.GGGGGCCAG.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattc                                <  1:2030090/93‑1 (MQ=17)
                                                                    attccGC.GGGGGGCCG.GGGGG.CAC.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcat                              <  2:2146246/97‑1 (MQ=17)
                                                                     atccGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt                             <  2:2765551/98‑1 (MQ=21)
                                                                     aagccggggggggCCG.GGGGG.CCG.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcat                              <  1:2573422/93‑1 (MQ=17)
                                                                         gC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCa                         >  1:4588877/1‑101 (MQ=255)
                                                                         gC.CGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCa                         >  2:2994272/1‑101 (MQ=255)
                                                                          gcggggggTCC.GGGGG.CAC.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCa                         <  2:229465/98‑1 (MQ=18)
                                                                          c.cGGTGGGCC.GGGGG.CGG.CG.CCCCCGGC.TTTTCTCAGATTAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCAt                        <  1:2116058/101‑1 (MQ=18)
                                                                            cGGGGGTCC.GGGGG.CGG.CT.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATa                       <  1:1567712/101‑1 (MQ=18)
                                                                             gggggTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAc                      >  1:233410/1‑101 (MQ=255)
                                                                             gggggTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAc                      >  1:2757593/1‑101 (MQ=255)
                                                                             gggggTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAc                      >  2:1136535/1‑101 (MQ=255)
                                                                              gggggcggggggg.CCC.CC.CCCCGGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAc                      <  1:49088/93‑1 (MQ=17)
                                                                              ggggTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAcc                     >  1:3408921/1‑101 (MQ=255)
                                                                               gggTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCa                    >  1:4266804/1‑101 (MQ=255)
                                                                                 tctc.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACg                  <  1:3949406/98‑1 (MQ=21)
                                                                                 gTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACg                  >  2:387712/1‑101 (MQ=255)
                                                                                  tCC.GGGGG.CAC.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGa                 <  2:1295628/101‑1 (MQ=21)
                                                                                    c.GGGGG.CAG.CGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGaga               >  2:3949121/1‑99 (MQ=18)
                                                                                      ggggg.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAg              >  2:1494692/1‑101 (MQ=255)
                                                                                       gggg.CAG.CGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAgg             >  2:3508750/1‑101 (MQ=255)
                                                                                       gggg.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAgg             >  1:2157957/1‑101 (MQ=255)
                                                                                          gtcgg.cg.cCCCTGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGAt           <  1:779291/97‑1 (MQ=18)
                                                                                             aG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCg        >  2:3263172/1‑101 (MQ=255)
                                                                                             aG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCg        >  2:892103/1‑101 (MQ=255)
                                                                                               gcccccccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCgg       <  2:247119/97‑1 (MQ=255)
                                                                                                cgcccccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGa      >  2:921160/4‑101 (MQ=255)
                                                                                                 tcccccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAc     <  1:239006/99‑1 (MQ=255)
                                                                                                 gcccccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAc     >  1:705876/3‑101 (MQ=255)
                                                                                                 ccccccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAc     <  1:1640859/99‑1 (MQ=255)
                                                                                                  cccccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAcc    <  1:2992/100‑1 (MQ=255)
                                                                                                  cccccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAcc    >  2:1818907/2‑101 (MQ=255)
                                                                                                  cccccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAca    >  2:238982/2‑100 (MQ=255)
                                                                                                   ccccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCa   >  1:2512365/1‑101 (MQ=255)
                                                                                                    cccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAt  >  1:2653671/1‑101 (MQ=255)
                                                                                                    cccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAt  <  2:836146/101‑1 (MQ=255)
                                                                                                       |                                                                                                   
TTGCCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.G.AAA.TACGC.CGGGGGTCC.GGGGG.CAG.CG.CCCCGGCG.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT  >  NC_014034/1976798‑1976990

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG