Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,976,894 C→G 89.3% intergenic (‑73/+7) fba ← / ← gap1 fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,976,8940C→G89.3% 35.4 0.0 0.0150intergenic (‑73/+7)fba/gap1fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (92/42):  ref base (11/5):  total (103/47)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

GCCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCGGCG.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT  >  NC_014034/1976800‑1976990
                                                                                                     |                                                                                                 
  cGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTtctc                                                                                          >  1:809996/1‑101 (MQ=255)
  cGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTtctc                                                                                          >  2:1165984/1‑101 (MQ=255)
     aGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGa                                                                                       >  1:3368100/1‑101 (MQ=255)
       ggTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CGACCCCGGC..TTTTCTCAGATc                                                                                     >  2:1011047/1‑101 (MQ=255)
       ggTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATc                                                                                     >  2:3925354/1‑101 (MQ=255)
       ggTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATc                                                                                     >  2:4016053/1‑101 (MQ=255)
       ggTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATc                                                                                     >  2:458765/1‑101 (MQ=255)
         tGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAg                                                                                   >  2:1834041/1‑101 (MQ=255)
          gATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGc                                                                                  >  2:2640615/1‑101 (MQ=255)
          gATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGc                                                                                  >  2:40721/1‑101 (MQ=255)
          gATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGc                                                                                  >  2:3474290/1‑101 (MQ=255)
            tCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCtt                                                                                >  2:683268/1‑101 (MQ=255)
               gTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGcc                                                                             >  1:1904731/1‑101 (MQ=255)
                tgcttttttCTCTTTT.CTTGGGTTCGCGGCTTTTTTTGTGG.CC.CAAA.ACCCGGGGGGGGCG.GGGGG.CCG.CC.CCCCCCGGCTTTTCTCAGATCAGCTTGccc                                                                            <  1:1884390/96‑1 (MQ=255)
                tgcttttttCTCTGTCCCTTGAG.TCGGCGCCTTTTTTTTGGCCC.AAAA.TCCGCGGGGGGCCG.GGGGG.CGG.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGccc                                                                            <  1:669645/96‑1 (MQ=255)
                    aTTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCGTCTTGCCCATCg                                                                        >  2:2070018/1‑101 (MQ=25)
                    aTTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCg                                                                        >  2:2358077/1‑101 (MQ=255)
                    aTTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCg                                                                        >  1:969745/1‑101 (MQ=255)
                    aTTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCg                                                                        >  1:4602498/1‑101 (MQ=255)
                    aTTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCg                                                                        >  2:751278/1‑101 (MQ=255)
                      tttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTGGG.CC.AAAA.TCCGCGGGGGGTCC.GGGGG.CAG.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGcc                                                                      <  1:2970811/101‑1 (MQ=25)
                            aGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCAcggcg                                                                >  1:3703873/1‑101 (MQ=255)
                            aGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCACATCGCCAcggcg                                                                >  1:3108311/1‑101 (MQ=37)
                              ttCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGt                                                              >  2:2202814/1‑101 (MQ=255)
                               tctttGGGTTCGC.GCCTTTTTTTTGG.CCAAAAA.CCCGCGGGAGGCCC.GGGGG.CCC.CC.CCCCGGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa                                                             <  1:3426563/98‑1 (MQ=9)
                               tCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa                                                             >  1:247143/1‑101 (MQ=255)
                                ccTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTAt                                                            >  2:2573248/1‑101 (MQ=255)
                                ccTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTAt                                                            >  1:1723324/1‑101 (MQ=255)
                                 ctggggTTCGGGGCTTTTTTCTGGG.CC.GAAA.TCCGCGGGGGGTCG.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATc                                                           <  2:2561886/96‑1 (MQ=21)
                                 cTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATc                                                           >  1:78589/1‑101 (MQ=255)
                                  ttGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCg                                                          <  2:2827969/101‑1 (MQ=255)
                                  ttGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCg                                                          >  1:1990047/1‑101 (MQ=255)
                                   tggttccgcgTCTTTTTTCTGGG.CC.AAAA.TACGCGGGGGGTCC.GGGGC.CCG.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCgg                                                         <  1:724694/95‑1 (MQ=16)
                                   tGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCgg                                                         >  1:2187997/1‑101 (MQ=255)
                                     aGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGcc                                                       >  1:49283/1‑101 (MQ=255)
                                     aGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGcc                                                       >  2:3295659/1‑101 (MQ=255)
                                      gTTCGCGGCCTTTTTCTTNG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CGC.CG.CCNCCGGC.TTTTCTCAGATCNNNNNGCCNCTCGNCANNTCCGCCTCGGCCa                                                      >  1:2847708/1‑101 (MQ=16)
                                      gTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCa                                                      >  1:4418614/1‑101 (MQ=255)
                                      gTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCa                                                      >  2:1330840/1‑101 (MQ=255)
                                      gTTCGCGGCCTTTTTCGGGG.CC.GAAA.TCCGCGGGGGGCCG.GGGGG.CCC.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCa                                                      <  1:2154429/101‑1 (MQ=21)
                                          gcgGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCg                                                  >  2:264994/1‑101 (MQ=255)
                                          gcgGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCg                                                  >  2:1511136/1‑101 (MQ=255)
                                           cgGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCgg                                                 >  2:4618469/1‑101 (MQ=255)
                                           cgGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCgg                                                 >  2:2035333/1‑101 (MQ=255)
                                            ggCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGt                                                >  1:3862456/1‑101 (MQ=255)
                                             gtcTTTTTCTGGG.CC.GAAA.TCCGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTAAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGtt                                               <  2:3794710/99‑1 (MQ=25)
                                             gcttttttCTTGT.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGtt                                               <  2:779238/98‑1 (MQ=37)
                                             gCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGtt                                               >  2:4215460/1‑101 (MQ=255)
                                              ccTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTc                                              >  2:802401/1‑101 (MQ=255)
                                               cTTTTTTTTGG.CC.AAAA.ACCCGCGGGGGGCG.GGGGGCCGG.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTc                                              <  1:4173960/101‑1 (MQ=12)
                                                tttttCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCga                                            >  2:462207/1‑101 (MQ=255)
                                                tttttCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGGTCga                                            >  2:3901438/1‑101 (MQ=37)
                                                tttttCGTTG.CC.CAAA.ATGGCCGGGGGGCCGGGGGG.CCG.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCg                                             <  1:661447/101‑1 (MQ=12)
                                                 ttttCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgag                                           >  2:4440804/1‑101 (MQ=255)
                                                     cTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGc                                       >  1:3235853/1‑101 (MQ=255)
                                                        gg.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcccc                                    >  2:1567843/1‑101 (MQ=255)
                                                        gg.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcccc                                    >  1:3297752/1‑101 (MQ=255)
                                                         g.CC.GAAATTCCGCGGGGGGTCG.GGGGG.CCC.CC.CCCCGGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcccc                                    <  1:4410992/101‑1 (MQ=18)
                                                           cc.GAAA.T.CCCCGGGGGGCC.GGGGG.CCGCCC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcat                                  <  2:3553793/101‑1 (MQ=17)
                                                            c.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcatt                                 >  1:1800480/1‑101 (MQ=255)
                                                            c.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcatt                                 >  2:1902401/1‑101 (MQ=255)
                                                              gAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattc                                >  2:2733762/1‑101 (MQ=255)
                                                              gAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcagat                                >  2:2444358/1‑98 (MQ=25)
                                                               aaa.TCCGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCGCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattca                               <  2:4341644/101‑1 (MQ=21)
                                                               aaa.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattca                               >  2:4178985/1‑101 (MQ=255)
                                                                gaaatcggcGGGGGGCC.GGGGGCCAG.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattc                                <  1:2030090/93‑1 (MQ=17)
                                                                aa.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcat                              >  1:4513782/1‑101 (MQ=255)
                                                                 acgcccgggggggCCC.GGGGGCCGG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattca                               <  1:4569918/93‑1 (MQ=17)
                                                                 aagccggggggggCCG.GGGGG.CCG.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcat                              <  1:2573422/93‑1 (MQ=17)
                                                                  atccGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt                             <  2:2765551/98‑1 (MQ=21)
                                                                   tACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGGCCCATTCAGTg                            >  2:1935351/1‑101 (MQ=21)
                                                                     cGCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTc                          <  2:3735040/101‑1 (MQ=255)
                                                                      gcggggggTCC.GGGGG.CAC.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCa                         <  2:229465/98‑1 (MQ=18)
                                                                      gCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCa                         >  2:2994272/1‑101 (MQ=255)
                                                                      gCCGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCa                         >  1:4588877/1‑101 (MQ=255)
                                                                       ccGGTGGGCC.GGGGG.CGG.CG.CCCCCGGC.TTTTCTCAGATTAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCAt                        <  1:2116058/101‑1 (MQ=18)
                                                                        cGGGGGTCC.GGGGG.CGG.CT.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATa                       <  1:1567712/101‑1 (MQ=18)
                                                                        cGGGGGTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCAATGCCCCCGGCGTTACCGCCCATGGGTTTGGAGAAGCCCCATTCATTgccctc                       >  2:3117432/1‑96 (MQ=16)
                                                                         gggggTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAc                      >  2:1136535/1‑101 (MQ=255)
                                                                         gggggTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAc                      >  1:233410/1‑101 (MQ=255)
                                                                         gggggTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAc                      >  1:2757593/1‑101 (MQ=255)
                                                                          ggggCCG.GGGGG.CGG.CC.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAcc                     <  1:3474548/101‑1 (MQ=18)
                                                                           gggTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCa                    <  2:3865757/101‑1 (MQ=255)
                                                                           gggTCC.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCa                    >  1:4266804/1‑101 (MQ=255)
                                                                             tctc.GGGGG.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACg                  <  1:3949406/98‑1 (MQ=21)
                                                                                    ggg.CAG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGa            >  2:3511646/1‑101 (MQ=255)
                                                                                         aG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCg        >  2:3263172/1‑101 (MQ=255)
                                                                                         aG.CG.CCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCg        >  2:892103/1‑101 (MQ=255)
                                                                                            cgcccccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGa      >  2:921160/4‑101 (MQ=255)
                                                                                             tcccccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAc     <  1:239006/99‑1 (MQ=255)
                                                                                             gcccccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCCTTGTCATACCACGACAGGATGCGGAc     >  1:2804192/3‑101 (MQ=255)
                                                                                             gcccccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAc     >  1:705876/3‑101 (MQ=255)
                                                                                             ccccccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAc     <  1:1640859/99‑1 (MQ=255)
                                                                                              cccccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAcc    <  1:2992/100‑1 (MQ=255)
                                                                                              cccccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAcc    >  2:1818907/2‑101 (MQ=255)
                                                                                              cccccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAca    >  2:238982/2‑100 (MQ=255)
                                                                                              cccccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTACCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAcc    >  2:3412343/2‑101 (MQ=255)
                                                                                               ccccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCa   >  1:2512365/1‑101 (MQ=255)
                                                                                                cccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAt  >  1:2653671/1‑101 (MQ=255)
                                                                                                cccGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAt  <  2:836146/101‑1 (MQ=255)
                                                                                                     |                                                                                                 
GCCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CC.GAAA.TACGCCGGGGGTCC.GGGGG.CAG.CG.CCCCGGCG.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT  >  NC_014034/1976800‑1976990

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG