Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,057,834 A→C 13.7% intergenic (‑600/‑302) RCAP_rcc01900 ← / → RCAP_rcc01901 hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,057,8340A→C13.7% 62.4 ‑1.3 ‑0.8176intergenic (‑600/‑302)RCAP_rcc01900/RCAP_rcc01901hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (14/10):  ref base (67/85):  total (81/95)
Fisher's exact test strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.53e-01

Only 100 of 184 total aligned reads displayed.

CTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CCGCG.CGC.AGC.G.TTCCGAACCGGCATCAATC.TTG.G.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057735‑2057913
                                                                                                       |                                                                                    
cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACCA.T.CC.CG.CGC.CGc                                                                                    >  2:3568007/1‑101 (MQ=255)
cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGc                                                                                    >  2:43422/1‑101 (MQ=255)
 tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGca                                                                                   >  1:931340/1‑100 (MQ=255)
 tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGca                                                                                   >  2:3847637/1‑100 (MQ=255)
  gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcca                                                                                  >  2:3247834/1‑99 (MQ=255)
  gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGAGTGGCGACAA.T.CC.CG.CGC.CGcaa                                                                                  >  2:775781/1‑99 (MQ=255)
   tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaag                                                                                 <  1:3492951/101‑4 (MQ=255)
   tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaag                                                                                 >  2:1547553/1‑98 (MQ=255)
   tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaag                                                                                 <  1:2281553/101‑4 (MQ=255)
    ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagc                                                                                >  1:382919/1‑97 (MQ=255)
    ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagc                                                                                >  2:2322517/1‑97 (MQ=255)
      aCCGCCCATTAACCCTGCTTCGGCGCGCGGACTGTGGTGCGGCGGCGGGGCTCTTGCAATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagcgt                                                                              <  1:3988518/101‑7 (MQ=255)
       ccGCCCATTCACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagcgtt                                                                             <  2:1511619/101‑8 (MQ=255)
       ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagcgtt                                                                             <  1:3877211/101‑8 (MQ=255)
       ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTCTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagcgtt                                                                             <  2:1679400/101‑8 (MQ=255)
       ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCCCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagcgtt                                                                             >  2:195513/1‑94 (MQ=255)
        cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagcgttt                                                                            >  1:4338024/1‑93 (MQ=255)
         gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagcgtttc                                                                           >  2:2456417/1‑92 (MQ=255)
         gCCCATTAACCCTGCTTCGGCGCGCGGACTNCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagcgtttc                                                                           <  1:366141/101‑10 (MQ=255)
                aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCg                                                                       >  2:2732235/1‑101 (MQ=255)
                   ccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCa                                                                    <  1:799349/101‑1 (MQ=255)
                     tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATc                                                                  >  2:596006/1‑101 (MQ=255)
                      gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCa                                                                 >  2:3918317/1‑101 (MQ=255)
                       cTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCTACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCat                                                                >  1:1507914/1‑100 (MQ=255)
                       cTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGGATCat                                                                >  2:4301480/1‑100 (MQ=255)
                       cTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCat                                                                <  1:2654415/101‑2 (MQ=255)
                       cTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCCGCGTTTCCGGATTGGCGTCAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCat                                                                <  1:1425074/101‑2 (MQ=255)
                        ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCatt                                                               <  1:3178504/101‑3 (MQ=255)
                         tCGGGGGGCCGACTTCGGGGCGGCGGGGGGGATCTTGCGGTCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTc                                                              <  2:1196421/101‑1 (MQ=255)
                          cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.t                                                            <  1:1559179/101‑1 (MQ=255)
                            gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTg                                                          <  2:228194/101‑1 (MQ=255)
                            gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTg                                                          <  1:1060503/101‑1 (MQ=255)
                              gcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.G.c                                                      >  2:2682838/1‑101 (MQ=255)
                                   gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.G.cccccc                                                 <  2:2162443/101‑2 (MQ=255)
                                   gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.G.cccccc                                                 <  1:3795382/101‑2 (MQ=255)
                                   gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.G.cccccc                                                 <  1:2812641/101‑2 (MQ=255)
                                    aCTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.G.ccccccg                                                >  2:3070965/1‑99 (MQ=255)
                                    aCTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.G.ccccccg                                                <  2:4197704/101‑3 (MQ=255)
                                      ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.G.ccccccgcc                                              >  2:3782285/1‑97 (MQ=255)
                                       tCGGGGGGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATGGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.G.ccccccgccg                                             <  2:2907565/101‑6 (MQ=255)
                                        cGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCgg                                             >  1:1681074/1‑101 (MQ=255)
                                         ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCggg                                            >  2:1406130/1‑101 (MQ=255)
                                          gggCGGCGGCGGGGCTCTTGCTATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCACCATTCTTTG.C.CCCCCGCCGGGa                                           <  2:1380751/101‑1 (MQ=255)
                                          gggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGa                                           <  1:740994/101‑1 (MQ=255)
                                           ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGAc                                          <  1:858102/101‑1 (MQ=255)
                                           ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGAc                                          <  2:254679/101‑1 (MQ=255)
                                            gcggcggcgGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACa                                         <  1:1974793/101‑1 (MQ=255)
                                            gcggcggcgGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACa                                         <  2:4505798/101‑1 (MQ=255)
                                             cggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACaa                                        <  1:2544858/101‑1 (MQ=255)
                                               gcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATc                                      <  2:1762320/101‑1 (MQ=255)
                                                 ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCAc                                    >  1:4538589/1‑101 (MQ=255)
                                                 ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCAc                                    <  2:376510/101‑1 (MQ=255)
                                                 ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCAc                                    >  2:847507/1‑101 (MQ=255)
                                                  gcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACg                                   <  1:2553074/101‑1 (MQ=255)
                                                    ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGgc                                 >  1:2349485/1‑101 (MQ=255)
                                                    ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGgc                                 <  1:2223199/101‑1 (MQ=255)
                                                    ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGgc                                 >  1:3053343/1‑101 (MQ=255)
                                                    ggggCTCTTGCGACCCTGCGTTTCCGGATTGGGGAAAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTGCC.CCCCCCCCGGGAAAATAACgac                                 >  2:3746061/1‑99 (MQ=255)
                                                       gCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGgccgc                              >  2:1286473/1‑101 (MQ=255)
                                                       gCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCACCGGGACAATCACGgccgc                              >  1:1660748/1‑101 (MQ=255)
                                                        ctctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGgccgcc                             >  2:3664757/1‑101 (MQ=255)
                                                        ctctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGgccgcc                             >  1:1158090/1‑101 (MQ=255)
                                                         tctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCt                            <  1:1734157/101‑1 (MQ=255)
                                                          ctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTc                           >  1:4076900/1‑101 (MQ=255)
                                                          ctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTc                           >  1:3574744/1‑101 (MQ=255)
                                                           ttGCGATCCGGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCa                          <  2:1182437/101‑1 (MQ=255)
                                                            tGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         >  2:3628313/1‑101 (MQ=255)
                                                            tGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         >  1:1868996/1‑101 (MQ=255)
                                                            tGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         >  2:523417/1‑101 (MQ=255)
                                                            tGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         >  2:1648723/1‑101 (MQ=255)
                                                            tGCGAGCCTGCGTTTCCGGATTGGCGCCAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         <  2:2133106/101‑1 (MQ=255)
                                                              cGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTg                       >  2:3392888/1‑101 (MQ=255)
                                                               gATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGt                      >  2:4313824/1‑101 (MQ=255)
                                                               gATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACAGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGt                      >  1:2370875/1‑101 (MQ=255)
                                                                agccTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGtt                     <  2:755356/99‑1 (MQ=255)
                                                                aTCCTGCGTTTCCGGATTGGCGACAA.TCCCCCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGtt                     <  1:1547658/101‑1 (MQ=255)
                                                                 tCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    <  1:2193385/101‑1 (MQ=255)
                                                                  ccTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                   >  2:3826536/1‑101 (MQ=255)
                                                                     gCGTGTCCGGATGGGCGACAACC.CCGCGCCGCAAGCGG.TTCCGAACCGGCGTCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc                <  2:4625734/101‑1 (MQ=255)
                                                                       gTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              <  2:4405878/101‑1 (MQ=255)
                                                                       gTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              >  1:2920656/1‑101 (MQ=255)
                                                                        tttCCGGATTGGCGACAACC.CCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             <  1:2732433/101‑1 (MQ=255)
                                                                        tttCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             >  2:563619/1‑101 (MQ=255)
                                                                          tCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCTAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGc           <  1:3248079/101‑1 (MQ=255)
                                                                          tCCGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGc           >  2:4067450/1‑101 (MQ=255)
                                                                           ccGGATTGGCAACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCg          >  2:1088544/1‑101 (MQ=255)
                                                                            cGGATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGa         >  1:3788859/1‑101 (MQ=255)
                                                                             ggATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  1:4493889/1‑101 (MQ=255)
                                                                             ggATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  2:2964413/1‑101 (MQ=255)
                                                                             ggATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  1:3719375/1‑101 (MQ=255)
                                                                              gATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc       >  2:3708322/1‑101 (MQ=255)
                                                                              gATTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc       >  2:4578295/1‑101 (MQ=255)
                                                                               aTTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      >  1:4395996/1‑101 (MQ=255)
                                                                               aTTGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      <  2:1865391/101‑1 (MQ=255)
                                                                               aTTGGCGACAA.TCCCACGCCGCAAGC.GTTTCCGAACCGGCAGCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      <  2:2226106/101‑1 (MQ=255)
                                                                                ttGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     >  1:2127862/1‑101 (MQ=255)
                                                                                ttGGCGAAAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     <  1:516339/101‑1 (MQ=255)
                                                                                 tGGCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    <  1:43428/101‑1 (MQ=255)
                                                                                  ggCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAg   <  1:2487176/101‑1 (MQ=255)
                                                                                   gCGACAA.TCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTC.TTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGc  <  1:957601/101‑1 (MQ=255)
                                                                                                       |                                                                                    
CTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CCGCG.CGC.AGC.G.TTCCGAACCGGCATCAATC.TTG.G.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057735‑2057913

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG