Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,440 G→C 15.1% S2T (AGC→ACC)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4400G→C15.1% 78.2 ‑0.3 ‑0.2212S2T (AGC→ACC) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (16/16):  ref base (97/83):  total (113/99)
Fisher's exact test strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.38e-01

Only 100 of 221 total aligned reads displayed.

CCGCCGACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGA  >  NC_014034/2155340‑2155538
                                                                                                    |                                                                                                  
  cccgCCCCGCCGGCGGTCGCCCGCTCCTTCTGATGCCCTGGGGCCCCATCACCGTCACCGCCCCCCGGGCGAAAAGCCGCGAAAGGGCAGGATCATGAGCg                                                                                                  <  1:557267/100‑1 (MQ=255)
   ccgACCCGGCCGCCGTCCCCCTC.CCCCCTGATGCCCGGGGGCGCCGTCCCCGTCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGAt                                                                                                <  1:2803394/101‑1 (MQ=255)
     gACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATg                                                                                               >  2:4450347/1‑101 (MQ=255)
       cccGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCGGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                             <  2:2782875/101‑1 (MQ=255)
        ccGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCaa                                                                                            >  2:3484719/1‑101 (MQ=255)
         gggcgggcggcGCCCCCCCCTTCTGATCCCCTCGGGCCCCCTCCCGCCCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAg                                                                                           <  1:1576497/91‑1 (MQ=255)
         cGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCTGCGAAAGGGCAGGATCATGAGCGATGCAAg                                                                                           <  1:712081/101‑1 (MQ=255)
         cGGCCGCGGTCCCCCTCCCCTTCTGATGCCCTCGGGCGCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAg                                                                                           <  1:2078295/101‑1 (MQ=255)
           gCCGGGGCCCCCCTCCCCTTCTGATGCCCTGGGGCCCCGCCACCGTCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAggg                                                                                         <  2:308600/101‑1 (MQ=255)
           gCCGCGGTCGCCCTCCCCTTCTGATGCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAggg                                                                                         <  1:2568505/101‑1 (MQ=255)
            ccGCGGCCCCCCTCCCCTTCTGATGCCCGCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgggg                                                                                        <  1:2978746/101‑1 (MQ=255)
                ggTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                    >  2:3747989/1‑101 (MQ=255)
                 gTCGCCCTCCCCTTCTGATCCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                   <  1:4328301/101‑1 (MQ=255)
                 gTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCACGGGGCCTCg                                                                                   >  2:148009/1‑101 (MQ=255)
                 gTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                   >  1:3963938/1‑101 (MQ=255)
                  tcccctcctccTTCTGACCCCCGCGGCCGCCGCCACCGCCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGt                                                                                  <  2:1675304/92‑1 (MQ=255)
                  tCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGt                                                                                  >  2:139668/1‑101 (MQ=255)
                  ccGCCCTCCCCTTCGGAGCCCCTCGGGCGCCGCCCCCGCCCCCCCCCGCGGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGt                                                                                  <  2:722476/100‑1 (MQ=255)
                   cccccTCCCCTGCTGGTGCCCGCGGGCGCGGTCACCGCCCCCCCCCGCCGGGCGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTa                                                                                 <  1:1015510/99‑1 (MQ=255)
                    gCCCTCACGTTCTGTTGCCCCGGGGCCCCGTCCCCGCCCCCGCCCGCCGGGGGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTag                                                                                <  1:1270340/101‑1 (MQ=255)
                     cccTCCCCTTCGGATGCCCTCGGGCGCCGCCCCCGCCCCCGCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTaga                                                                               <  1:2416665/101‑1 (MQ=255)
                      ccTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                              >  1:1621009/1‑101 (MQ=255)
                        cccctttCTGATCCCCTGGGGCGCCGCCCCCCCCCCCGCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCt                                                                            <  1:4621489/96‑1 (MQ=255)
                         ccccTTCTGATCCCCCGGGGCGCCGTCCCCGTCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtc                                                                           <  2:3614094/99‑1 (MQ=255)
                           ccTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgt                                                                         >  1:2123241/1‑101 (MQ=255)
                              tCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                      >  2:1772417/1‑101 (MQ=255)
                               cTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGAt                                                                     >  2:3843275/1‑101 (MQ=255)
                                  gggccggcGGGCGCCGCCCCCGCCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGcc                                                                  <  2:4004401/94‑1 (MQ=255)
                                     cccTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATc                                                               >  2:270670/1‑101 (MQ=255)
                                         cGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggg                                                           >  2:4200499/1‑101 (MQ=255)
                                          gggCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGAGCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgggg                                                          >  2:4471971/1‑101 (MQ=255)
                                           ggCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggc                                                         >  1:1148220/1‑101 (MQ=255)
                                            gcgcCTTCCCCGTCCCCCCCCGCGGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcg                                                        <  2:631666/101‑1 (MQ=255)
                                                 gcccccgcccccgcccgcCGGGCGACAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                   <  2:209438/91‑1 (MQ=255)
                                                  ccccctcccccgcccgcGGGGGGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCg                                                  <  2:4138295/92‑1 (MQ=255)
                                                    accgtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTc                                                >  2:1142445/1‑101 (MQ=255)
                                                       gtccccgCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                             <  1:3722297/101‑1 (MQ=255)
                                                       ccccccccccgccGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                             <  1:3748264/94‑1 (MQ=255)
                                                         caccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcaccac                                           >  2:1967055/1‑101 (MQ=255)
                                                          cccgccgccCGGGCTAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACg                                          <  2:480752/100‑1 (MQ=255)
                                                            cgcccgccGGGGGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGAt                                        <  2:1791042/101‑1 (MQ=255)
                                                             ccccgcgGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATg                                       <  1:216993/100‑1 (MQ=255)
                                                               ccgcggGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggc                                     <  1:2003607/101‑1 (MQ=255)
                                                                ccgcgGGCAGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGCCGATGCCATCGTGGGCGGGGCCGTCACCACGATggcg                                    <  2:4239053/98‑1 (MQ=255)
                                                                 gccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcgg                                   >  1:1930573/1‑101 (MQ=255)
                                                                 gccgGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcgg                                   >  2:3752375/1‑101 (MQ=255)
                                                                  ccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggc                                  >  2:2563993/1‑101 (MQ=255)
                                                                  ccgGGCGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggc                                  <  1:3523029/101‑1 (MQ=255)
                                                                   cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                 >  1:2634859/1‑101 (MQ=255)
                                                                    gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                >  2:1109561/1‑101 (MQ=255)
                                                                     ggcggcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcggc                               >  2:2650484/1‑101 (MQ=255)
                                                                       cggcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGgcgc                             >  1:2944497/1‑101 (MQ=255)
                                                                         gcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                           >  2:3276594/1‑101 (MQ=255)
                                                                          cAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCg                          >  1:4535664/1‑101 (MQ=255)
                                                                           aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGc                         >  2:3388113/1‑101 (MQ=255)
                                                                            cgCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGATCTCGTCGATGCCATCTGGGGTGGGGCCGTCACGACCAGGGCGGCGGGGCTCGcg                        >  2:1164835/2‑100 (MQ=255)
                                                                            aGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGcc                        <  1:3843560/101‑1 (MQ=255)
                                                                            aGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGcc                        <  1:3143072/101‑1 (MQ=255)
                                                                                 cgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcg                   >  2:2209379/1‑101 (MQ=255)
                                                                                  gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                  <  2:563461/101‑1 (MQ=255)
                                                                                  gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                  >  1:4063633/1‑101 (MQ=255)
                                                                                   aaaTGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                 <  2:2311288/101‑1 (MQ=255)
                                                                                   aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                 >  1:1674335/1‑101 (MQ=255)
                                                                                   aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggccgc                 >  2:38011/1‑101 (MQ=255)
                                                                                   aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCCCGCCGggcggc                 >  2:2851276/1‑101 (MQ=255)
                                                                                    aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                >  1:1228187/1‑101 (MQ=255)
                                                                                    aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGACCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                <  1:2884163/101‑1 (MQ=255)
                                                                                      gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGa              <  2:2362406/101‑1 (MQ=255)
                                                                                         gaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgt           <  1:4207072/94‑1 (MQ=255)
                                                                                         gaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgg           >  2:1634535/8‑100 (MQ=255)
                                                                                          aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg          >  1:2351967/1‑101 (MQ=255)
                                                                                          aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg          >  1:2772264/1‑101 (MQ=255)
                                                                                           ggATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTgg         >  1:3104175/1‑101 (MQ=255)
                                                                                           aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTgg         <  1:4228372/96‑1 (MQ=255)
                                                                                           aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTgg         <  2:3196677/96‑1 (MQ=255)
                                                                                           aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCCGGCCGGGCGCCTGATTTgg         >  2:3688282/6‑101 (MQ=255)
                                                                                            gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGc        <  2:3103931/101‑1 (MQ=255)
                                                                                            gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGc        >  2:2986981/1‑101 (MQ=255)
                                                                                            aaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGc        <  2:3005858/97‑1 (MQ=255)
                                                                                             aaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCa       >  2:1285160/4‑101 (MQ=255)
                                                                                             aTCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCa       >  1:600917/1‑101 (MQ=255)
                                                                                             aTCATGAGCGATGCAAGGGGCCTCGGAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCa       >  2:138238/1‑101 (MQ=255)
                                                                                              aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt      >  1:2732377/3‑101 (MQ=255)
                                                                                              aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt      <  1:4490333/99‑1 (MQ=255)
                                                                                               cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCGTCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt     >  1:4569126/1‑101 (MQ=255)
                                                                                               cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt     <  2:1593829/101‑1 (MQ=255)
                                                                                               cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt     <  2:3963653/101‑1 (MQ=255)
                                                                                               aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt     >  2:3331650/2‑101 (MQ=255)
                                                                                                aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGGGCTCGCCGGGCGGCTGATGTGGCATTc    >  1:1701217/1‑101 (MQ=255)
                                                                                                aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc    <  2:389449/101‑1 (MQ=255)
                                                                                                aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc    >  1:1523980/1‑101 (MQ=255)
                                                                                                aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc    <  1:4424547/101‑1 (MQ=255)
                                                                                                aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc    >  1:1341878/1‑101 (MQ=255)
                                                                                                aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc    >  2:3140056/1‑101 (MQ=255)
                                                                                                 tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGGGCTCGCCGGGCGGCTGATGTGGCAtttg   >  2:1358379/1‑99 (MQ=255)
                                                                                                 tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg   <  1:2486834/101‑1 (MQ=255)
                                                                                                 tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg   <  1:2274501/101‑1 (MQ=255)
                                                                                                 tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg   >  1:3401303/1‑101 (MQ=255)
                                                                                                 tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg   <  1:3647684/101‑1 (MQ=255)
                                                                                                 tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg   >  1:880663/1‑101 (MQ=255)
                                                                                                    |                                                                                                  
CCGCCGACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGA  >  NC_014034/2155340‑2155538

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG