Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,447 A→C 17.8% A4A (GCA→ACC RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4470A→C17.8% 112.2 ‑1.9 ‑0.5213A4A (GCA→GCCRCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (16/22):  ref base (98/77):  total (114/99)
Fisher's exact test strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.89e-01

Only 100 of 225 total aligned reads displayed.

CCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGC  >  NC_014034/2155347‑2155547
                                                                                                    |                                                                                                    
cccGGCCGCGGCCGCCCCCTCCTTCTGATCCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                                      <  1:2751064/101‑1 (MQ=255)
 ccGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCaa                                                                                                     >  2:3484719/1‑101 (MQ=255)
   ggCCGCGGTCCCCCTCCCCTTCTGATGCCCTCGGGCGCCGTCCCCGTCACCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgg                                                                                                   <  1:1479480/101‑1 (MQ=255)
    gCCGCGGTCGCCCTCCCCTTCTGATGCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAggg                                                                                                  <  1:2568505/101‑1 (MQ=255)
     ccGCGGCCCCCCTCCCCTTCTGATGCCCGCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgggg                                                                                                 <  1:2978746/101‑1 (MQ=255)
        cgGTCGCCCTCCCCTTCTGATCCCCTCGGGCGCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCt                                                                                              <  1:2448786/101‑1 (MQ=255)
        cgGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCt                                                                                              >  1:2002513/1‑101 (MQ=255)
        cgGGCGCCCTCCCTTTCTGAGCCCCGCGGGCGCCGTCCCCGTCACCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCt                                                                                              <  2:1024826/101‑1 (MQ=255)
         ggTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGCCCTc                                                                                             >  2:1549967/1‑101 (MQ=255)
          gTCGCCCTCCCCTTCTGATCCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                            <  1:4328301/101‑1 (MQ=255)
          gTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                            >  1:3963938/1‑101 (MQ=255)
           ccGCCCTCCCCTTCGGAGCCCCTCGGGCGCCGCCCCCGCCCCCCCCCGCGGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGt                                                                                           <  2:722476/100‑1 (MQ=255)
            ccccccccccTTCTGATCCCCTCGGGCGCCGCCCCCGTCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTa                                                                                          <  1:2022145/93‑1 (MQ=255)
            cccccTCCCCTGCTGGTGCCCGCGGGCGCGGTCACCGCCCCCCCCCGCCGGGCGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTa                                                                                          <  1:1015510/99‑1 (MQ=255)
             gCCCTCACGTTCTGTTGCCCCGGGGCCCCGTCCCCGCCCCCGCCCGCCGGGGGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTag                                                                                         <  1:1270340/101‑1 (MQ=255)
             gCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGGTCATGAGCGATGCAAGGGGCCTCGTag                                                                                         >  2:3459703/1‑101 (MQ=255)
               ccTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                       >  1:1621009/1‑101 (MQ=255)
                cTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGc                                                                                      >  1:2338857/1‑101 (MQ=255)
                  ccccTTCTGATCCCCCGGGGCGCCGTCCCCGTCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtc                                                                                    <  2:3614094/99‑1 (MQ=255)
                       tCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                               >  2:3071734/1‑101 (MQ=255)
                        cTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGAt                                                                              >  2:3843275/1‑101 (MQ=255)
                        cTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGAt                                                                              >  1:105437/1‑101 (MQ=255)
                             gCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCAt                                                                         >  2:852211/1‑101 (MQ=255)
                              cccTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATc                                                                        >  2:270670/1‑101 (MQ=255)
                                     gcgcCTTCCCCGTCCCCCCCCGCGGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcg                                                                 <  2:631666/101‑1 (MQ=255)
                                     gcgcCGCCCCCGCCCCCCCCCGCGGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcg                                                                 <  2:1613279/101‑1 (MQ=255)
                                          gtcaccgtcaccGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                            >  1:4593875/1‑101 (MQ=255)
                                          gcccccgcccccgcccgcCGGGCGACAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                            <  2:209438/91‑1 (MQ=255)
                                           tcaccgtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCg                                                           >  2:4455547/1‑101 (MQ=255)
                                           ccccctcccccgcccgcGGGGGGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCg                                                           <  2:4138295/92‑1 (MQ=255)
                                             accgtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTc                                                         >  2:1142445/1‑101 (MQ=255)
                                                gtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                                      >  2:2883947/1‑101 (MQ=255)
                                                ccccccccccgccGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                                      <  1:3748264/94‑1 (MQ=255)
                                                 tccccgccggcCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacca                                                     <  2:1460468/98‑1 (MQ=255)
                                                 cccccccccgcgGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacca                                                     <  2:3763986/95‑1 (MQ=255)
                                                   cccgcccgcCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACg                                                   <  1:2861165/100‑1 (MQ=255)
                                                      ccccgcgGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATg                                                <  1:216993/100‑1 (MQ=255)
                                                        ccgccgGGGGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggc                                              <  2:1177780/101‑1 (MQ=255)
                                                         ccgcgGGCAGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGCCGATGCCATCGTGGGCGGGGCCGTCACCACGATggcg                                             <  2:4239053/98‑1 (MQ=255)
                                                           ccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggc                                           >  2:2563993/1‑101 (MQ=255)
                                                              ggcggcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcggc                                        >  2:2650484/1‑101 (MQ=255)
                                                                cggcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGgcgc                                      >  1:2944497/1‑101 (MQ=255)
                                                                  gcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                    >  2:3276594/1‑101 (MQ=255)
                                                                  aaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                    <  2:3904860/99‑1 (MQ=255)
                                                                   cAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCg                                   >  2:3979170/1‑101 (MQ=255)
                                                                   cAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCg                                   >  1:4535664/1‑101 (MQ=255)
                                                                    aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTGGTAGAGCTAGTCGATGCCATCTGGGGCGGGGCCGTCCCCACGATGGCGGCGGCGCTCGc                                  >  1:770862/1‑101 (MQ=255)
                                                                    aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGc                                  >  2:1789506/1‑101 (MQ=255)
                                                                       tctcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCgg                               <  1:662955/98‑1 (MQ=255)
                                                                          cgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcg                            >  2:2209379/1‑101 (MQ=255)
                                                                           gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                           <  2:563461/101‑1 (MQ=255)
                                                                           gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                           >  1:4063633/1‑101 (MQ=255)
                                                                            aaaTGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                          <  2:2311288/101‑1 (MQ=255)
                                                                            aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                          >  1:2404243/1‑101 (MQ=255)
                                                                            aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                          >  1:1674335/1‑101 (MQ=255)
                                                                            aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggccgc                          >  2:38011/1‑101 (MQ=255)
                                                                             aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                         >  1:1228187/1‑101 (MQ=255)
                                                                             aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGACCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                         <  1:2884163/101‑1 (MQ=255)
                                                                               gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGa                       <  2:2362406/101‑1 (MQ=255)
                                                                               gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGa                       >  2:4573718/1‑101 (MQ=255)
                                                                               gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGa                       >  1:3652296/1‑101 (MQ=255)
                                                                                ggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTggt                      >  1:1573992/1‑99 (MQ=255)
                                                                                ggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAt                      >  2:1793293/1‑101 (MQ=255)
                                                                                 gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                     <  1:1772536/101‑1 (MQ=255)
                                                                                  gaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgt                    <  1:4207072/94‑1 (MQ=255)
                                                                                  gaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgg                    >  2:1634535/8‑100 (MQ=255)
                                                                                  cAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgt                    <  1:3315153/101‑1 (MQ=255)
                                                                                    aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTgg                  <  1:4228372/96‑1 (MQ=255)
                                                                                    aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTgg                  <  2:3196677/96‑1 (MQ=255)
                                                                                    aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCCGGCCGGGCGCCTGATTTgg                  >  2:3688282/6‑101 (MQ=255)
                                                                                     gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGTGGCGGCGCTCGCCGGGCGGCTGATGTGGc                 >  2:2833742/1‑101 (MQ=255)
                                                                                     gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGc                 >  1:2108490/1‑101 (MQ=255)
                                                                                     gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGc                 >  2:2986981/1‑101 (MQ=255)
                                                                                     gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGc                 <  2:3103931/101‑1 (MQ=255)
                                                                                      aaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCa                >  2:1285160/4‑101 (MQ=255)
                                                                                      aTCATGAGCGATGCAAGGGGCCTCGGAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCa                >  2:138238/1‑101 (MQ=255)
                                                                                       aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt               <  2:1116426/99‑1 (MQ=255)
                                                                                       aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt               <  1:4490333/99‑1 (MQ=255)
                                                                                        cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt              <  2:1723737/101‑1 (MQ=255)
                                                                                        cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt              >  2:3752188/1‑101 (MQ=255)
                                                                                        aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt              >  2:3331650/2‑101 (MQ=255)
                                                                                         aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGGGCTCGCCGGGCGGCTGATGTGGCATTc             >  1:1701217/1‑101 (MQ=255)
                                                                                         aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc             <  1:2603356/101‑1 (MQ=255)
                                                                                         aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCCGGGGGCTGATGTGGCAttg             >  2:448253/1‑100 (MQ=255)
                                                                                         aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc             <  1:4424547/101‑1 (MQ=255)
                                                                                         aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc             >  1:1341878/1‑101 (MQ=255)
                                                                                         aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc             >  1:3131167/1‑101 (MQ=255)
                                                                                          tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGGGCTCGCCGGGCGGCTGATGTGGCAtttg            >  2:1358379/1‑99 (MQ=255)
                                                                                          tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg            <  1:3820970/101‑1 (MQ=255)
                                                                                          tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg            >  2:4016974/1‑101 (MQ=255)
                                                                                          tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg            >  1:3401303/1‑101 (MQ=255)
                                                                                           gAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGa           >  1:594445/1‑101 (MQ=255)
                                                                                            accGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAc          <  2:2435997/99‑1 (MQ=255)
                                                                                             ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAcc         <  1:470738/100‑1 (MQ=255)
                                                                                             ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAcc         <  1:3279275/100‑1 (MQ=255)
                                                                                               gATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGa       >  1:226498/1‑101 (MQ=255)
                                                                                               gATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGa       <  1:2694996/101‑1 (MQ=255)
                                                                                                aTGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAg      >  1:1183724/1‑101 (MQ=255)
                                                                                                  gCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAggg    >  2:1866100/1‑100 (MQ=255)
                                                                                                   cAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTg   >  1:3617644/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGC  >  NC_014034/2155347‑2155547

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG