Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,483 C→T 38.5% G16G (GGC→GGT RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4830C→T38.5% 0.0 ‑0.5 ‑0.1291G16G (GGC→GGTRCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (66/46):  ref base (99/80):  total (165/126)
Fisher's exact test strand distribution p-value = 6.27e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.98e-01

Only 100 of 311 total aligned reads displayed.

GGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTG  >  NC_014034/2155383‑2155582
                                                                                                     |                                                                                                   
ggCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggc                                                                                                     >  1:1148220/1‑101 (MQ=255)
 gcgcCTTCCCCGTCCCCCCCCGCGGGGCGG.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcg                                                                                                    <  2:631666/101‑1 (MQ=255)
 gcgcCGCCCCCGCCCCCCCCCGCGGGGCGG.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcg                                                                                                    <  2:1613279/101‑1 (MQ=255)
             cccccccccgccGGGCGG.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacca                                                                                        <  2:2694474/95‑1 (MQ=255)
              caccgCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcaccac                                                                                       >  2:1967055/1‑101 (MQ=255)
               cccgccgccCGGGCTA.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACg                                                                                      <  2:480752/100‑1 (MQ=255)
                ccgcccggCGGGCGGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACg                                                                                      <  2:4211672/101‑1 (MQ=255)
                     ccgcgGGCAG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGCCGATGCCATCGTGGGCGGGGCCGTCACCACGATggcg                                                                                <  2:4239053/98‑1 (MQ=255)
                       ccgGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggc                                                                              >  2:2563993/1‑101 (MQ=255)
                          ggcgg.cAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcggc                                                                           >  2:2650484/1‑101 (MQ=255)
                              g.cAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                                                       >  1:4267816/1‑101 (MQ=255)
                                cAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCg                                                                      >  1:4535664/1‑101 (MQ=255)
                                cAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCg                                                                      >  2:3979170/1‑101 (MQ=255)
                                 aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGc                                                                     >  2:3388113/1‑101 (MQ=255)
                                  cgCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGATCTCGTCGATGCCATCTGGGGTGGGGCCGTCACGACCAGGGCGGCGGGGCTCGcg                                                                    >  2:1164835/2‑100 (MQ=255)
                                  aGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGcc                                                                    <  1:3843560/101‑1 (MQ=255)
                                        gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                                                              <  2:563461/101‑1 (MQ=255)
                                         aaaTGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                                                             <  2:2311288/101‑1 (MQ=255)
                                         aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                                                             >  1:1674335/1‑101 (MQ=255)
                                         aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                                                             >  1:2404243/1‑101 (MQ=255)
                                         aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggccgc                                                             >  2:38011/1‑101 (MQ=255)
                                            gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGa                                                          <  2:2362406/101‑1 (MQ=255)
                                            gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGa                                                          >  2:4573718/1‑101 (MQ=255)
                                            ggagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGa                                                          <  1:2792180/91‑1 (MQ=255)
                                             ggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAt                                                         <  2:2123091/101‑1 (MQ=255)
                                             ctgaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAt                                                         <  1:3614510/92‑1 (MQ=255)
                                              gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                                                        <  1:1772536/101‑1 (MQ=255)
                                               cAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgt                                                       <  1:3315153/101‑1 (MQ=255)
                                               cAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgt                                                       <  1:2564167/101‑1 (MQ=255)
                                                 aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTgg                                                     <  2:3196677/96‑1 (MQ=255)
                                                 aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTgg                                                     <  1:4228372/96‑1 (MQ=255)
                                                  gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGTGGCGGCGCTCGCCGGGCGGCTGATGTGGc                                                    >  2:2833742/1‑101 (MQ=255)
                                                  gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGc                                                    >  2:2986981/1‑101 (MQ=255)
                                                  aaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGc                                                    <  2:1288123/97‑1 (MQ=255)
                                                  aaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGc                                                    <  1:1334903/97‑1 (MQ=255)
                                                   aTCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCa                                                   >  1:600917/1‑101 (MQ=255)
                                                    aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt                                                  <  1:2589384/99‑1 (MQ=255)
                                                     cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCGTCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt                                                 >  1:4569126/1‑101 (MQ=255)
                                                     cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt                                                 <  2:1723737/101‑1 (MQ=255)
                                                     cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt                                                 >  2:3752188/1‑101 (MQ=255)
                                                      aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGGGCTCGCCGGGCGGCTGATGTGGCATTc                                                >  1:1701217/1‑101 (MQ=255)
                                                      aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc                                                <  2:389449/101‑1 (MQ=255)
                                                      aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc                                                >  1:3131167/1‑101 (MQ=255)
                                                         accGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAc                                             <  2:2435997/99‑1 (MQ=255)
                                                          ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAcc                                            <  1:470738/100‑1 (MQ=255)
                                                          ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAcc                                            <  1:3279275/100‑1 (MQ=255)
                                                            gataccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGa                                          >  1:905421/10‑101 (MQ=255)
                                                            gATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGa                                          <  1:1021790/101‑1 (MQ=255)
                                                            gATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGa                                          >  1:226498/1‑101 (MQ=255)
                                                            gATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGa                                          <  1:2694996/101‑1 (MQ=255)
                                                             aTGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAg                                         >  2:74680/1‑101 (MQ=255)
                                                             aTGCAAGGGGCATCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAg                                         >  2:1134603/1‑101 (MQ=255)
                                                               gCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAggg                                       >  2:1866100/1‑100 (MQ=255)
                                                               accagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAggg                                       >  1:3739457/7‑100 (MQ=255)
                                                               accagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGt                                       >  1:2284262/7‑101 (MQ=255)
                                                                ccagaggCCTCGTCGAGGTCATCGATGCCCTATGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTg                                      >  2:1660641/6‑101 (MQ=255)
                                                                  agaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCg                                    >  2:850532/4‑101 (MQ=255)
                                                                  agaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCg                                    >  1:1236447/4‑101 (MQ=255)
                                                                  agaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCg                                    >  1:3678146/4‑101 (MQ=255)
                                                                  aGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCg                                    <  2:453761/101‑1 (MQ=255)
                                                                   ggggCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCgg                                   >  1:1227476/1‑101 (MQ=255)
                                                                   gaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCgg                                   >  1:528606/3‑101 (MQ=255)
                                                                    aggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCggg                                  <  2:3650754/100‑1 (MQ=255)
                                                                    aggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCggg                                  <  2:905341/100‑1 (MQ=255)
                                                                     ggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgc                                 >  1:3815100/1‑101 (MQ=255)
                                                                     ggCCTCGTAGAGCTCGTCGATGCCATCTGGGGCTGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGgc                                 <  1:4021273/101‑1 (MQ=255)
                                                                     ggCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGgc                                 >  2:1791048/1‑101 (MQ=255)
                                                                        cTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGgcgcg                              <  1:3484977/101‑1 (MQ=255)
                                                                          cGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcggc                            <  2:534985/101‑3 (MQ=255)
                                                                          cGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCa                            >  1:793802/1‑101 (MQ=255)
                                                                           gtcgagGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcggcg                           >  2:2245803/4‑98 (MQ=255)
                                                                             cgagGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGGGCGGgcgcggcggc                         >  2:1554826/2‑96 (MQ=255)
                                                                             agagCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAgc                         <  2:1224332/101‑1 (MQ=255)
                                                                             agagCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAgc                         <  2:4048690/101‑1 (MQ=255)
                                                                              gagGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcggcggcc                        >  1:4109467/1‑95 (MQ=255)
                                                                              gagGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcggccgcg                        >  2:2893326/1‑95 (MQ=255)
                                                                              gagGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcgccggcg                        >  2:1423806/1‑96 (MQ=255)
                                                                              gagGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGGGCGGgcgcggcggcg                        >  2:2030702/1‑95 (MQ=255)
                                                                               agCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTTCGGGCGCGCAAgcgc                       >  1:2087065/1‑101 (MQ=255)
                                                                                  tcgtcgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGcgccgc                    >  1:2628629/1‑101 (MQ=255)
                                                                                  tcatcgATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGGGCGGGCGCGGCGGcgccgg                    >  2:3115355/4‑100 (MQ=255)
                                                                                   cgtcgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCt                   <  1:4455842/101‑1 (MQ=255)
                                                                                    atcgATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGGGCGGGCGCGGCGGCGCCGGtt                  >  2:3010888/2‑101 (MQ=255)
                                                                                      cgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTcc                >  2:3948593/1‑101 (MQ=255)
                                                                                      cgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTcc                >  1:2899505/1‑101 (MQ=255)
                                                                                       gATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCt               <  2:3556092/101‑1 (MQ=255)
                                                                                       gATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCt               >  1:1935523/1‑101 (MQ=255)
                                                                                       gATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCt               >  1:2141111/1‑101 (MQ=255)
                                                                                        aTGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGtttttc              >  1:397816/1‑97 (MQ=255)
                                                                                        aTGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGttgttc              >  2:3690694/1‑97 (MQ=255)
                                                                                          gCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAg            >  2:1380571/1‑101 (MQ=255)
                                                                                            cATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGcc          <  1:2987210/101‑1 (MQ=255)
                                                                                             aTCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCcg         <  1:3092315/101‑1 (MQ=255)
                                                                                             aTCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCcg         <  2:3524792/101‑1 (MQ=255)
                                                                                              tCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAGGCGCCGCTTCCTGAGCcgc        >  2:4449470/1‑101 (MQ=255)
                                                                                              tCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCcgc        >  2:432565/1‑101 (MQ=255)
                                                                                               cTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCcgcg       >  2:2495998/1‑101 (MQ=255)
                                                                                                tGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGa      >  1:1400984/1‑101 (MQ=255)
                                                                                                 ggggcggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAGTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAg     >  1:3923477/1‑101 (MQ=255)
                                                                                                   ggcggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAgct   <  2:3300751/101‑1 (MQ=255)
                                                                                                     |                                                                                                   
GGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTG  >  NC_014034/2155383‑2155582

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG