Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,157,926 C→A 92.8% intergenic (‑298/‑484) RCAP_rcc02004 ← / → RCAP_rcc02005 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,157,9260C→A92.8% 37.9 ‑2.2 0.0208intergenic (‑298/‑484)RCAP_rcc02004/RCAP_rcc02005hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (99/94):  ref base (11/4):  total (110/98)
Fisher's exact test strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

CTCCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.ACGGCCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCCGTCGTC  >  NC_014034/2157834‑2158021
                                                                                             |                                                                                               
ctcCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCg                                                                                         <  1:593863/101‑1 (MQ=255)
   ccAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGtt                                                                                      >  1:2504625/1‑101 (MQ=255)
   ccAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGtt                                                                                      <  2:4572266/101‑1 (MQ=255)
    cAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTc                                                                                     >  1:2427702/1‑101 (MQ=255)
    cAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTc                                                                                     <  1:1882749/101‑1 (MQ=255)
    cAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTCCGGTTc                                                                                     >  1:766050/1‑101 (MQ=38)
    cAGCAGGCCTAACCGCTGGCGACCATCAGGTTCACCCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTc                                                                                     <  2:711958/101‑1 (MQ=37)
     aGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCa                                                                                    <  1:3556830/101‑1 (MQ=255)
      gCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGACAGACCAATGAAAATC.ACCCGATTCGGTTCAg                                                                                   >  2:4229383/1‑101 (MQ=37)
       cATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGt                                                                                  <  1:1731100/101‑1 (MQ=255)
        aTACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTc                                                                                 >  1:4010316/1‑101 (MQ=255)
          aCCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTccg                                                                               <  2:3714888/101‑1 (MQ=255)
          aCCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTccg                                                                               >  2:2123660/1‑101 (MQ=255)
           ccgaaCCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTccgc                                                                              >  1:2658471/4‑101 (MQ=38)
             aaaCCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTccgccg                                                                            >  1:697201/1‑101 (MQ=255)
             aaaCCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTccgccg                                                                            >  1:4184645/1‑101 (MQ=255)
             aaaCCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTccgccg                                                                            >  1:1273378/1‑101 (MQ=255)
             aaaCCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTAGGTTCAGTccgccg                                                                            >  2:2018220/1‑101 (MQ=38)
              aaCCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCcgt                                                                           >  2:151042/1‑101 (MQ=255)
              aaCCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCcgt                                                                           <  1:3970438/101‑1 (MQ=255)
               aCCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCcgtc                                                                          <  1:3046444/101‑1 (MQ=255)
               aCCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCcgtc                                                                          >  1:75088/1‑101 (MQ=255)
                 cGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCcgtcgt                                                                        >  2:1711069/1‑101 (MQ=255)
                 cGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCcgtcgt                                                                        >  2:2433648/1‑101 (MQ=255)
                  gCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCgtcgtc                                                                       >  1:298898/1‑101 (MQ=255)
                  gCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCgtcgtc                                                                       >  2:3390805/1‑101 (MQ=255)
                   cTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCt                                                                      <  2:2553356/101‑1 (MQ=255)
                   cTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCt                                                                      >  1:2726868/1‑101 (MQ=255)
                     ggCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTg                                                                    >  1:4590866/1‑101 (MQ=255)
                     ggCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGCCTTg                                                                    >  1:438121/1‑101 (MQ=38)
                       cGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGc                                                                  >  2:3116141/1‑101 (MQ=255)
                       cGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGc                                                                  <  1:1126663/101‑1 (MQ=255)
                        gACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCg                                                                 >  1:2541306/1‑101 (MQ=255)
                        gACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCg                                                                 >  2:756900/1‑101 (MQ=255)
                        gACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGGCTTGGCg                                                                 >  2:4033137/1‑101 (MQ=38)
                         aCCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCgg                                                                >  1:3203802/1‑101 (MQ=255)
                          ccATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGt                                                               >  2:1957/1‑101 (MQ=255)
                          ccATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGt                                                               <  1:1313110/101‑1 (MQ=255)
                            agcAGGTTCAGCCCGCCGTCGTCTTGGCGGTGATGTGATGGACAGGATAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGa                                                             <  2:3296185/99‑1 (MQ=37)
                             tCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGAc                                                            <  1:3886863/101‑1 (MQ=255)
                              cAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACg                                                           <  2:2591481/101‑1 (MQ=255)
                              cAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACg                                                           >  2:3500034/1‑101 (MQ=255)
                               aGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGt                                                          >  2:55933/1‑101 (MQ=255)
                               aGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGGGACGt                                                          >  1:1434754/1‑101 (MQ=38)
                                ggTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTg                                                         <  1:4546711/101‑1 (MQ=255)
                                ggTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTg                                                         >  1:1220544/1‑101 (MQ=255)
                                  ttCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGGGACGTGAg                                                       >  2:2512621/1‑101 (MQ=38)
                                    taGTCCGCCGTCGTCTTGGGGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGc                                                     <  2:833291/100‑1 (MQ=38)
                                     actCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCa                                                    <  2:4266504/99‑1 (MQ=255)
                                     aGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCa                                                    <  2:2002241/101‑1 (MQ=255)
                                      gTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCAc                                                   >  1:1370723/1‑101 (MQ=255)
                                          gccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTaa                                               >  2:2237869/1‑101 (MQ=255)
                                            cgtcgtCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAAc                                             >  2:1298180/1‑101 (MQ=255)
                                             gtcgtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACa                                            >  1:1671594/1‑101 (MQ=255)
                                                gtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAaga                                         >  2:3897280/1‑101 (MQ=255)
                                                 tcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAagag                                        >  1:3488009/1‑101 (MQ=255)
                                                  cTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAgg                                       >  2:4197684/1‑101 (MQ=255)
                                                      gCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCt                                   <  2:4081409/101‑1 (MQ=255)
                                                      gCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCt                                   <  1:3318835/101‑1 (MQ=255)
                                                       cGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCTCCTAAACAAGAGGTTCTa                                  <  2:378127/101‑1 (MQ=38)
                                                       cGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTa                                  <  1:2564897/101‑1 (MQ=255)
                                                          tgatgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGa                               <  2:804332/101‑1 (MQ=255)
                                                          tgatgggatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGa                               <  2:3420169/101‑1 (MQ=38)
                                                           gatgtgatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGAt                              >  2:1602355/1‑101 (MQ=255)
                                                           gatgtgatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGAt                              >  2:4448447/1‑101 (MQ=255)
                                                           gatgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGAt                              >  2:202366/1‑101 (MQ=255)
                                                           gatgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGAt                              <  1:1842769/101‑1 (MQ=255)
                                                           gatgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGAt                              >  1:1048151/1‑101 (MQ=255)
                                                            atgtgatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATg                             >  1:1012160/1‑101 (MQ=255)
                                                            atgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATg                             >  1:3392940/1‑101 (MQ=255)
                                                             tgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGc                            <  1:790123/101‑1 (MQ=255)
                                                               tgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCg                          <  2:2542237/101‑1 (MQ=255)
                                                               tgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCg                          <  2:2715360/101‑1 (MQ=255)
                                                               tgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCg                          <  2:75082/101‑1 (MQ=255)
                                                               tgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCg                          <  2:1305929/101‑1 (MQ=255)
                                                                gatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGa                         <  2:2280609/101‑1 (MQ=255)
                                                                gatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGa                         <  1:3862982/101‑1 (MQ=255)
                                                                 atgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGaa                        >  2:2069184/1‑101 (MQ=255)
                                                                 atgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGNGGTTCTACGATGGCGaa                        >  2:779747/1‑101 (MQ=38)
                                                                  tgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGaaa                       >  2:4117700/1‑101 (MQ=255)
                                                                  tgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGaaa                       <  1:4293488/101‑1 (MQ=255)
                                                                    cacaGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAgg                     >  1:1634234/1‑101 (MQ=255)
                                                                     acaGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAggg                    >  2:3185817/1‑101 (MQ=255)
                                                                     acaGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCAGGTAAACAAGAGGTTCTACGATGCCGAAAggg                    <  1:4151274/101‑1 (MQ=38)
                                                                       aGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGt                  >  2:4341105/1‑101 (MQ=255)
                                                                       aGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGt                  <  1:2229753/101‑1 (MQ=255)
                                                                        ggAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGtt                 >  1:3192236/1‑101 (MQ=255)
                                                                         gagagaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTc                <  2:4601670/101‑1 (MQ=255)
                                                                         gagagaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGATc                >  2:1428914/1‑101 (MQ=38)
                                                                          agagaCCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGNGGntga               >  1:565485/1‑99 (MQ=37)
                                                                          agagaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCa               >  2:2869876/1‑101 (MQ=255)
                                                                           gagaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAg              <  1:2123810/101‑1 (MQ=255)
                                                                            agaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGt             <  1:451085/101‑1 (MQ=255)
                                                                             gaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTc            <  1:2014009/101‑1 (MQ=255)
                                                                              aCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTcc           <  2:1762038/101‑1 (MQ=255)
                                                                               gcGAAGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTccg          <  2:2421398/100‑1 (MQ=38)
                                                                                 gATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTccgcc        <  2:1012077/101‑1 (MQ=255)
                                                                                     aaaaTC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgtcg    >  1:479787/1‑101 (MQ=255)
                                                                                     aaaaTC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgtcg    >  1:3688069/1‑101 (MQ=255)
                                                                                      aaaTC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgtcgt   >  1:2361064/1‑101 (MQ=255)
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CTCCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.ACGGCCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCCGTCGTC  >  NC_014034/2157834‑2158021

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG