Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,176,472 G→C 100% A302G (GCG→GGG)  rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,176,4720G→C100.0% 923.0 243A302G (GCG→GGG) rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (116/127):  ref base (0/0):  total (116/127)

Only 100 of 254 total aligned reads displayed.

CCCAGCATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATG.CCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCGCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGTGGC  >  NC_014034/2176375‑2176569
                                                                                                  |                                                                                                 
  cAGCATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTc                                                                                               <  1:109285/101‑1 (MQ=255)
  cAGCATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTc                                                                                               <  2:4532870/101‑1 (MQ=255)
   aGCATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCg                                                                                              <  1:4103502/101‑1 (MQ=255)
    gCATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGcccgcagg                                                                                             >  1:2290561/1‑97 (MQ=255)
     cATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGa                                                                                            >  2:2221490/1‑101 (MQ=255)
     cATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGa                                                                                            >  1:436304/1‑101 (MQ=255)
       ttGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTAACCCCGGTGTTGACGAAATCGAGCCCGTCGGAAt                                                                                          >  2:1503435/1‑101 (MQ=255)
         gTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTc                                                                                        >  2:3487747/1‑101 (MQ=255)
         gTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTc                                                                                        <  1:3547654/101‑1 (MQ=255)
         gTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTc                                                                                        >  2:561206/1‑101 (MQ=255)
         gTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTc                                                                                        <  2:699339/101‑1 (MQ=255)
          tttCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCa                                                                                       >  2:3745142/1‑101 (MQ=255)
          tttCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCa                                                                                       <  2:4488091/101‑1 (MQ=255)
           ttCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCAc                                                                                      <  1:631368/101‑1 (MQ=255)
            tCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCAcc                                                                                     >  2:2747126/1‑101 (MQ=255)
            tCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCAcc                                                                                     <  2:3829245/101‑1 (MQ=255)
             cAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCg                                                                                    <  2:4238357/101‑1 (MQ=255)
               gcgcCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGc                                                                                  <  1:746285/101‑1 (MQ=255)
                 gcCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCTTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCtt                                                                                <  2:2062160/101‑1 (MQ=255)
                 gcCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCtt                                                                                >  2:2806692/1‑101 (MQ=255)
                  ccTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTc                                                                               <  1:3038917/101‑1 (MQ=255)
                    tctTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGt                                                                             >  2:1543001/1‑101 (MQ=255)
                    tctTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGt                                                                             >  1:1994791/1‑101 (MQ=255)
                      ttCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGc                                                                           <  2:4224271/101‑1 (MQ=255)
                      ttCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGc                                                                           >  1:4491804/1‑101 (MQ=255)
                        ccGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCg                                                                         >  1:3478211/1‑101 (MQ=255)
                         tgAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTNCCgn                                                                        >  1:3315828/2‑100 (MQ=255)
                          gAGGTGATCGAGGGGGTGCCCCCGATCGGCCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTc                                                                       <  2:1653328/101‑1 (MQ=255)
                          gAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTc                                                                       >  2:3712661/1‑101 (MQ=255)
                          gAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTc                                                                       <  2:3187544/101‑1 (MQ=255)
                          gAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTc                                                                       >  1:1694995/1‑101 (MQ=255)
                           aGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTcc                                                                      >  1:361490/1‑101 (MQ=255)
                            ggTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCt                                                                     <  1:1372790/101‑1 (MQ=255)
                                aTCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGc                                                                 >  2:1705539/1‑101 (MQ=255)
                                aTCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGc                                                                 >  2:4312569/1‑101 (MQ=255)
                                  cGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCAt                                                               <  2:240510/101‑1 (MQ=255)
                                   gAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATa                                                              >  1:3951699/1‑101 (MQ=255)
                                   gAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATa                                                              >  1:3725709/1‑101 (MQ=255)
                                   gAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATa                                                              >  1:2853612/1‑101 (MQ=255)
                                    aGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAg                                                             <  1:894046/101‑1 (MQ=255)
                                     ggggATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGGCCTTGGCATAgg                                                            >  2:1874708/1‑101 (MQ=255)
                                      gggATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAggc                                                           >  1:993727/1‑101 (MQ=255)
                                      gggATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAggc                                                           <  1:1322342/101‑1 (MQ=255)
                                         atgcccccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAggcggc                                                        >  1:2169208/5‑101 (MQ=255)
                                          tgcccccGATCGGACTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGgcggcg                                                       <  2:3447590/98‑1 (MQ=255)
                                           gcccccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGa                                                      <  1:248644/99‑1 (MQ=255)
                                            cccccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGAt                                                     >  2:1074467/2‑101 (MQ=255)
                                            cccccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGGGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGGGGCGac                                                     >  2:2888477/2‑100 (MQ=255)
                                            cccccGAGCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGAt                                                     <  2:1796164/100‑1 (MQ=255)
                                             ccccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATc                                                    >  2:299594/1‑101 (MQ=255)
                                             ccccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATc                                                    <  2:3394783/101‑1 (MQ=255)
                                             ccccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATc                                                    <  2:2538548/101‑1 (MQ=255)
                                              cccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCg                                                   <  2:436267/101‑1 (MQ=255)
                                                  aTCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGGGATCGCCTc                                               >  1:1121647/1‑101 (MQ=255)
                                                  aTCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTc                                               <  2:2293442/101‑1 (MQ=255)
                                                   tCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCg                                              <  1:1527701/101‑1 (MQ=255)
                                                   tCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCg                                              <  1:121094/101‑1 (MQ=255)
                                                     gggCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGAt                                            <  2:3560711/101‑1 (MQ=255)
                                                      ggCTGGCGGTGACCAGCGTCACCCGGGTGTGCACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATg                                           <  2:497098/101‑1 (MQ=255)
                                                        cTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGcc                                         <  2:1293710/101‑1 (MQ=255)
                                                         tGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGccc                                        <  2:1007842/101‑1 (MQ=255)
                                                           tCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCtt                                      <  2:2526864/101‑1 (MQ=255)
                                                            cGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTTGGAATTCACCGGCTTCGTGCCGACCTTGGCATAGGCGTCGATCGCCTCGATGCCCTTg                                     >  1:1817961/1‑101 (MQ=255)
                                                             ggTGACCAGCGTCACCCCGGTGTTGACGAAACCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTgg                                    <  1:2375217/101‑1 (MQ=255)
                                                               tGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAg                                  <  1:4456052/101‑1 (MQ=255)
                                                               tGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAg                                  >  1:1601876/1‑101 (MQ=255)
                                                                 aCCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGc                                >  2:2126130/1‑101 (MQ=255)
                                                                 aCCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGc                                <  1:1389823/101‑1 (MQ=255)
                                                                 aCCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGAGCGCCTCGATGCCCTTGGAGGc                                >  1:67184/1‑101 (MQ=255)
                                                                  ccAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGcc                               <  1:126224/101‑1 (MQ=255)
                                                                    aGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCAt                             <  2:3800967/101‑1 (MQ=255)
                                                                    aGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCAt                             <  2:400274/101‑1 (MQ=255)
                                                                    aGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCAt                             >  1:2647668/1‑101 (MQ=255)
                                                                      cGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCa                           >  1:1081445/1‑101 (MQ=255)
                                                                       gTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATccg                          >  2:3973318/1‑99 (MQ=255)
                                                                       gTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAg                          >  1:3263470/1‑101 (MQ=255)
                                                                        tCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGa                         <  1:349441/101‑1 (MQ=255)
                                                                         cACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGaa                        >  2:92408/1‑101 (MQ=255)
                                                                          aCCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAg                       <  1:4208493/101‑1 (MQ=255)
                                                                              cGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCggg                   >  1:3845114/1‑101 (MQ=255)
                                                                               ggTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGa                  <  1:1543107/101‑1 (MQ=255)
                                                                               ggTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGa                  <  2:2309115/101‑1 (MQ=255)
                                                                               ggTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGa                  <  2:60243/101‑1 (MQ=255)
                                                                                gtgtTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGaa                 <  2:1332306/101‑1 (MQ=255)
                                                                                 tgtTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAAt                <  1:2712373/101‑1 (MQ=255)
                                                                                 tgtTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAAt                >  1:2189772/1‑101 (MQ=255)
                                                                                    tGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGc             <  2:4626953/101‑1 (MQ=255)
                                                                                      aCGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCAt           <  1:570013/101‑1 (MQ=255)
                                                                                      aCGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCAt           >  2:4202514/1‑101 (MQ=255)
                                                                                      aCGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCAt           >  2:2484978/1‑101 (MQ=255)
                                                                                       cGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATc          <  1:92422/101‑1 (MQ=255)
                                                                                       cGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATc          >  2:3574676/1‑101 (MQ=255)
                                                                                       cGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATc          <  1:2907342/101‑1 (MQ=255)
                                                                                         aaaTCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGa        <  2:1230122/101‑1 (MQ=255)
                                                                                           aTCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAgg      <  1:3565216/101‑1 (MQ=255)
                                                                                            tCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGt     <  2:446847/101‑1 (MQ=255)
                                                                                             cGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCATTCGCATAGGCGGCGATCGCCTTGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGTg    <  1:3988564/101‑1 (MQ=255)
                                                                                              gAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGTgg   <  2:1170361/101‑1 (MQ=255)
                                                                                              gAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGTgg   >  2:2939992/1‑101 (MQ=255)
                                                                                               aGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGTGgc  <  1:3805533/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CCCAGCATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATG.CCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCGCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGTGGC  >  NC_014034/2176375‑2176569

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG