Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,186,012	G→A	100%	intergenic (+285/‑220)	mtlK → / → RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,186,012	0	G→A	100.0%	116.3	33	intergenic (+285/‑220)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (23/9):  ref base (1/0):  total (24/9)

GCCGAACCGCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCC.GGGGGCGGAG..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGAA  >  NC_014034/2185922‑2186109                                                                                              |                                                                                                  ccgacccccccccTTCCCCCTTGGCTCAAATACCCCGGGGGTCC.GGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAgc                                                                                         <  1:4396627/92‑1 (MQ=2)                tccctttGGCTCAAATACCCGGGGGGTGC.GGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACtt                                                                          <  2:4653221/96‑1 (MQ=2)                              attccccGGGGGGCCGGGGGGCGGGGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTc                                                             <  2:1677032/97‑1 (MQ=9)                                                    ggcgcccccGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggcag                                       <  2:272140/95‑1 (MQ=21)                                                           cggccgGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCt                                <  2:828167/99‑1 (MQ=37)                                                                         ctcgggcaaaGCCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAccc                    >  1:2090371/9‑101 (MQ=255)                                                                         ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGGAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAccc                    >  1:3243625/9‑101 (MQ=255)                                                                         ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAccc                    >  2:1385014/9‑101 (MQ=255)                                                                         ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAccc                    >  1:2134597/9‑101 (MQ=255)                                                                         ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAccc                    >  1:433633/9‑101 (MQ=255)                                                                          tcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCt                   <  1:3474525/94‑1 (MQ=255)                                                                          tcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCt                   >  2:2922719/8‑101 (MQ=255)                                                                           cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt                  <  2:888844/95‑1 (MQ=255)                                                                            gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc                 >  1:4209696/6‑101 (MQ=255)                                                                            gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc                 >  1:1695785/6‑101 (MQ=255)                                                                             ggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCg                >  1:543746/5‑101 (MQ=255)                                                                             ggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCg                >  2:2873211/5‑101 (MQ=255)                                                                             ggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCg                >  2:3148061/5‑101 (MQ=255)                                                                              gcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCgg               <  1:1385109/98‑1 (MQ=255)                                                                               caaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGa              >  2:3539157/3‑101 (MQ=255)                                                                               caaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGa              >  1:2475029/3‑101 (MQ=255)                                                                                aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc             >  2:1786249/2‑101 (MQ=255)                                                                                 aaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAcc            >  1:841170/1‑101 (MQ=255)                                                                                 aaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAcc            >  2:907658/1‑101 (MQ=255)                                                                                  agcccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCg           >  1:331811/4‑101 (MQ=255)                                                                                      cGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGtt       >  1:3014732/1‑101 (MQ=255)                                                                                      cGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGCCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCgggct       >  1:1933861/1‑99 (MQ=255)                                                                                       naGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACNNNNtnn      >  1:695064/2‑99 (MQ=255)                                                                                        aGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTgg     >  2:3673929/1‑101 (MQ=255)                                                                                        aGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTgg     <  2:838305/101‑1 (MQ=255)                                                                                        aGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGGTgg     >  2:3916926/1‑101 (MQ=255)                                                                                        aGGCGAAACACCCCAGCGACCCCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTgg     <  2:2229571/101‑1 (MQ=255)                                                                                         ggCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTggg    >  2:3359377/1‑101 (MQ=255)                                                                                           cGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGaa  >  2:3536019/1‑101 (MQ=255)                                                                                              |                                                                                                  GCCGAACCGCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCC.GGGGGCGGAG..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGAA  >  NC_014034/2185922‑2186109 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG