Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 222,078 C→G 2.5% T193T (ACG→ACC)  RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034222,0780C→G 2.5% 11.6 0.0 ‑0.3410T193T (ACG→ACC) RCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/5):  ref base (200/198):  total (206/204)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.73e-01

Only 100 of 693 total aligned reads displayed.

GGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGC  >  NC_014034/221973‑222178
                                                                                                         |                                                                                                    
     cgggcggctccggaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtcc                                                                                                      <  2:1848073/101‑1 (MQ=11)
      ggtcggatccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGGTCCGTGGGGTCGgtggggtacg                                                                                                     >  2:2821684/1‑101 (MQ=11)
        tcgggtccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGGGGGATCGGTCGGGTACGTGGGAGCGgtcggggccgtg                                                                                                   >  2:1735947/1‑100 (MQ=11)
        tcgggtccgtcggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtc                                                                                                   <  1:125622/101‑1 (MQ=11)
        tcgggtccggggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcggatccgtg                                                                                                   <  2:1176909/101‑2 (MQ=11)
        tcggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtc                                                                                                   <  1:3950285/101‑1 (MQ=255)
        gcgggtccgtcggatcggtgggATCCGTGGGGTCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtg                                                                                                   <  1:2418842/100‑2 (MQ=11)
         cggctccgtcggttcggagggGGCCGTGGGGTCGGTCGAGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCCgtggggtccgtcg                                                                                                  <  2:4382536/101‑1 (MQ=11)
         cggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcg                                                                                                  >  2:1583020/1‑101 (MQ=255)
          gggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgacgggtgcggggg                                                                                                 >  2:2601970/1‑97 (MQ=11)
           gatccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGGGGGGTCGGTCGGGTCCGTGGGGTCGgtcgggtcggtgggg                                                                                                >  2:1463039/3‑101 (MQ=11)
           gatccgtaggatgggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgtcggg                                                                                                <  1:1003866/99‑1 (MQ=11)
            atccgtgggatcggtcggATCCGTAGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTTGGATCGgtcggttccgctgtac                                                                                               >  2:230767/2‑95 (MQ=11)
            agcggtcgggtcggtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgggt                                                                                               <  2:4657572/91‑1 (MQ=11)
              tctagggatcgggcggCTCCGTGGGATCGGTCGGGTCCGTTGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtggggtcc                                                                                             <  2:2691167/97‑1 (MQ=11)
              ccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtggggtcc                                                                                             <  2:3969085/101‑1 (MQ=11)
              ccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcggatcg                                                                                             <  2:1253427/101‑2 (MQ=255)
               cgtgggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtcggatcgg                                                                                            >  1:2671544/1‑99 (MQ=11)
               cgggggagcggtcggGTCCGGGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGtcggatccgtggggtccg                                                                                            <  1:4591072/98‑1 (MQ=14)
                 tgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTcgggtccgtcggatcggtc                                                                                          >  1:2104185/1‑100 (MQ=11)
                  gggctcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTgg                                                                                         <  1:201320/101‑1 (MQ=11)
                  gggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGGTCCGTCGGCCCGGGcgggtcgctggcgtccgtcg                                                                                         >  2:2145995/1‑99 (MQ=1)
                  cgggtccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTgg                                                                                         >  2:3157847/11‑101 (MQ=11)
                  cgggtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTgg                                                                                         >  1:443950/8‑101 (MQ=11)
                  aggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGCCCGTCGGACCCGTGGGGGCCGTGGGGCCCGTgc                                                                                         >  1:4550379/2‑100 (MQ=2)
                   gggtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGGCGGGTCCGTCGGATCGGTggg                                                                                        >  1:2826741/7‑101 (MQ=11)
                   ggacccctgcgATCCTTGGGATTGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgg                                                                                        <  2:654023/91‑1 (MQ=1)
                    ggtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTGCGGACCGTCGGAGCCGTGGGa                                                                                       >  1:147304/6‑101 (MQ=11)
                    ggtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGa                                                                                       <  2:4103016/96‑1 (MQ=11)
                    ggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGCGGGa                                                                                       >  1:2499852/6‑101 (MQ=11)
                     atcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGCTCGGTCGGGTCCGTCGGATCCGGGGGGGCCGTCGGGTCCGTgggcc                                                                                      >  1:4182674/1‑99 (MQ=14)
                      gccgtgggATCTGTCCGATCCGTTGGGGCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtc                                                                                     <  2:553149/95‑1 (MQ=14)
                       ggggcggggccGTGGGATCGGTCGGGTCCGTTGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtcc                                                                                    <  1:696197/92‑2 (MQ=11)
                       cggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcg                                                                                    <  1:2727372/101‑1 (MQ=14)
                       ccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcg                                                                                    >  2:4236490/6‑101 (MQ=14)
                        ggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTGGGATCCGTGGGGTCCGTGGGAtcgg                                                                                   >  2:2522445/1‑101 (MQ=11)
                        ggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGGGGGAccgg                                                                                   >  2:2025311/1‑101 (MQ=11)
                        ggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTACGGCGGGTCGGTagggttcg                                                                                   >  2:4449267/1‑96 (MQ=11)
                        ggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtccg                                                                                   >  1:2860732/1‑99 (MQ=11)
                        cgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtccg                                                                                   >  2:59154/2‑99 (MQ=11)
                          tggggtCCGTGGGGTCGGTCGGGTCCGTGGGGTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcggtc                                                                                 <  1:3201062/96‑1 (MQ=11)
                          tcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCGGTGGGGTCCGTGGGAtcggcc                                                                                 >  2:3659141/1‑99 (MQ=11)
                          tcggATCCGTGGGATCGGTCGGATCCGGGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGGGGGAGCGGTCGGGGCCCGGGGGGCCGTCGGAtccgtg                                                                                 >  2:2924872/1‑100 (MQ=11)
                          gcggGTCCGTAGGAATGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtc                                                                                 <  2:2892977/100‑1 (MQ=14)
                           cggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtccgtgg                                                                                >  2:3214313/1‑99 (MQ=11)
                            ggATCGGTCGGATCCGTGGGGGCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcggtcgg                                                                               <  1:4666147/101‑1 (MQ=17)
                            ggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTTGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtccgtggg                                                                               <  1:754465/101‑1 (MQ=11)
                              aTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGAt                                                                             >  1:1705289/1‑101 (MQ=11)
                              aTCCGTGGGATCGGCCGGAGCCGTGGGATCGGTCGGATCCGGGGGATCGGTCGGGTCCGTGGGATGGGTCGGGGCCGTGGGGTCCGCCGGATCCGTGGGAt                                                                             >  2:386572/1‑101 (MQ=11)
                                ccggaggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTcc                                                                           <  2:3018579/96‑1 (MQ=14)
                                ccGTGGGATGGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTcc                                                                           <  1:1792634/101‑1 (MQ=14)
                                ccGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTcc                                                                           <  1:1555648/101‑1 (MQ=14)
                                 ggtcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCg                                                                          <  1:354585/97‑1 (MQ=18)
                                  gtcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCgg                                                                         >  1:2207289/4‑100 (MQ=17)
                                    gggATCGGTCGGGTCGGTGGGATCGGTCGGATCGGTGGGATCGGTGGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTgg                                                                       <  1:908217/101‑1 (MQ=14)
                                    gggATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTgg                                                                       >  1:3414375/1‑101 (MQ=14)
                                    cggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGCCCGTGGGATCGGTCGGATCCGTgg                                                                       >  2:2463002/2‑101 (MQ=18)
                                    aggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGtcg                                                                       >  1:256266/2‑99 (MQ=14)
                                    aggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGGCCGTCGGCTCCGTGGGGTCCGTCGGGGCCGTGGGATCGGTCGGGTCCGtcg                                                                       >  2:3377988/2‑99 (MQ=11)
                                      gATCGGTCGGGTCCGTGGGATCCGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGa                                                                     <  2:1349737/101‑1 (MQ=17)
                                       gtccgtggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAt                                                                    <  2:85152/94‑1 (MQ=255)
                                       aTCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAt                                                                    >  1:1967427/1‑101 (MQ=255)
                                         cggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTGGGGTCCGTCGGAtcg                                                                  >  2:1040878/1‑101 (MQ=14)
                                           gtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGGCCGTCGGAtcggt                                                                >  1:2997382/1‑101 (MQ=14)
                                             tggattggggggATCGGGCGGGTCCGTGGGATCGGTCGGTTCCGTGGGATCG.........GTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgg                                                     <  2:925181/92‑1 (MQ=14)
                                               ggTCCGTGGGATGGGTCGGATCCGGGGGATCGGACGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCggg                                                            <  1:4031746/101‑1 (MQ=17)
                                                 tCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTc                                                          <  1:4356118/101‑1 (MQ=17)
                                                  ccGTGGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTcc                                                         <  1:1624718/101‑1 (MQ=17)
                                                   cGTGGGATCTGGCGGACCCGTGGGATCGGTGGGGTCCGTGGGGTCCGGGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCg                                                        <  2:3319341/101‑1 (MQ=2)
                                                    gTGGGGTCGGTCGGGGCCGTGGGATCGGTCGGGTCCGTAGGGTCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGt                                                       <  1:1196382/101‑1 (MQ=18)
                                                    gTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGt                                                       >  2:503723/1‑101 (MQ=17)
                                                     tGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTg                                                      >  1:2934503/1‑101 (MQ=255)
                                                     tGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGta                                                      >  1:611778/1‑100 (MQ=17)
                                                      cggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGGTCGGTCGGGTCCGTCGGATCGGTCGGGTCCGGgg                                                     >  2:1968857/2‑101 (MQ=17)
                                                      cggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGGCCGTgg                                                     >  1:104119/2‑101 (MQ=17)
                                                       ggATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTggg                                                    >  1:3043151/1‑101 (MQ=255)
                                                       ggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTggg                                                    <  2:2764530/101‑1 (MQ=17)
                                                         aTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggat                                                  <  2:3446796/101‑3 (MQ=14)
                                                          tcggtcggATCCGTGGGATCGGCCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGCGGGTTCGGTCGGGTCCGTGTGGTCGGTCGGGCCCGTaggctc                                                 >  1:3259262/1‑98 (MQ=14)
                                                             gtcggGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGGCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtggt                                              <  1:3891231/101‑1 (MQ=255)
                                                              tcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGGCCGTCGGATCGGGCGGGTCCGTgggatcgggg                                             >  1:2342895/1‑94 (MQ=14)
                                                               cggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcggtcg                                            >  2:3070588/1‑93 (MQ=14)
                                                               cggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtcg                                            >  1:4102190/1‑93 (MQ=11)
                                                                ggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGAgggatcggtcgg                                           >  1:138866/1‑92 (MQ=11)
                                                                 ggtCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTgggg                                          >  2:3910557/3‑100 (MQ=255)
                                                                 ggtCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGa                                          >  1:3580600/3‑101 (MQ=255)
                                                                 gATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGGCGGGTCCGTgggatcgggcggg                                          >  1:3674423/1‑91 (MQ=11)
                                                                    ccGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcg                                       >  1:3775256/1‑101 (MQ=255)
                                                                         ggATCGGTCGGGTTCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcggtcgg                                  <  1:3128357/101‑1 (MQ=255)
                                                                         ggATCGGTCGGGTCCGTAGGTTCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcggtcgg                                  <  2:403402/101‑1 (MQ=255)
                                                                          gATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGa                                 >  1:428654/1‑101 (MQ=255)
                                                                              ggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGc                             >  2:4498975/1‑101 (MQ=255)
                                                                                     tCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgcc                      <  2:1673343/101‑1 (MQ=255)
                                                                                       cGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGTGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGCTCGCTGTcgctgc                    >  2:738680/1‑101 (MQ=25)
                                                                                        gTAGGAGCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGCGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgccgcc                   <  2:2380150/101‑1 (MQ=255)
                                                                                           ggATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCgccgccgcc                >  2:2237881/1‑101 (MQ=255)
                                                                                                  tcgggtcggtggggtccgTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGa         <  1:859293/101‑1 (MQ=255)
                                                                                                    gggtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCTCTGTCGCCGCCGCCGGACAGAcc       <  2:357315/101‑1 (MQ=255)
                                                                                                      gtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCa     >  1:564015/1‑101 (MQ=255)
                                                                                                         cgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAggc  >  2:4182061/1‑101 (MQ=255)
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GGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGC  >  NC_014034/221973‑222178

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG