Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 222,081 C→G 24.4% P192P (CCG→CCC RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034222,0810C→G24.4% 248.7 ‑0.2 ‑0.0409P192P (CCG→CCCRCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (49/51):  ref base (147/162):  total (196/213)
Fisher's exact test strand distribution p-value = 8.19e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.94e-01

Only 100 of 632 total aligned reads displayed.

GGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGG  >  NC_014034/221973‑222181
                                                                                                            |                                                                                                    
         gggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcg                                                                                                     <  1:2768350/97‑1 (MQ=255)
         cggatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgtcg                                                                                                     <  1:3065660/101‑1 (MQ=11)
          gggtccggggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcggatccgtggg                                                                                                    <  1:173160/101‑1 (MQ=11)
          ggatccgtggggtccgtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGggggggtccgtcgg                                                                                                    >  2:586071/4‑101 (MQ=11)
          ggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgg                                                                                                    >  1:855326/4‑101 (MQ=255)
          ggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgg                                                                                                    >  1:3760059/4‑101 (MQ=255)
          ggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgg                                                                                                    >  1:1830145/4‑101 (MQ=255)
          ggatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgtcgg                                                                                                    >  1:916074/4‑101 (MQ=11)
           gatccgtggtatcgggcggATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGCTCCGTAGGATCGGTCGGGTCCGTCGGATCGgtcggatccgtggga                                                                                                   <  2:3132974/99‑2 (MQ=11)
           gatccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGGGGGGTCGGTCGGGTCCGTGGGGTCGgtcgggtcggtgggg                                                                                                   >  2:1463039/3‑101 (MQ=11)
           gatccgtaggatgggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgtcggg                                                                                                   <  1:1003866/99‑1 (MQ=11)
              ccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcggatcg                                                                                                <  2:1253427/101‑2 (MQ=255)
                gtgggatcggtcggATCCGTGGTATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGCCGGGACCGTAGGATCGgtcgggtccgtgggatcggc                                                                                              >  1:3133218/1‑98 (MQ=11)
                gtgggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTcgggtccgtcggatccgt                                                                                              >  1:483340/1‑101 (MQ=11)
                gtgggagcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgggtccgtggggtccgt                                                                                              <  1:2867469/101‑1 (MQ=11)
                gtggcgtcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgggtccgtggggtccgt                                                                                              <  2:53305/95‑1 (MQ=11)
                 tgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTcgggtccgtcggatcggtc                                                                                             >  1:2104185/1‑100 (MQ=11)
                  gggctcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTgg                                                                                            <  1:201320/101‑1 (MQ=11)
                  aggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGCCCGTCGGACCCGTGGGGGCCGTGGGGCCCGTgc                                                                                            >  1:4550379/2‑100 (MQ=2)
                   gggtccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTggg                                                                                           <  2:3026582/92‑1 (MQ=14)
                   gggtccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTggg                                                                                           >  2:3180602/10‑101 (MQ=11)
                   ggggccgtcggATCGGGGGGGTCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTggg                                                                                           <  1:4449562/95‑1 (MQ=11)
                   ggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGGGCCGGGGGGTCGGGCGGGTCCGTCGGCTGCGTggg                                                                                           >  1:1587745/1‑101 (MQ=14)
                    gatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGGAGGGTCCGTgggg                                                                                          >  2:2366497/1‑100 (MQ=11)
                    gatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTGGGATCCGTAGGATCGGTCGAGTAAGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGa                                                                                          >  1:3985769/1‑101 (MQ=11)
                     gtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGag                                                                                         >  2:269094/5‑100 (MQ=11)
                      tcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtc                                                                                        <  2:855250/101‑1 (MQ=14)
                       ccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcg                                                                                       <  1:706315/96‑1 (MQ=14)
                        ggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCCGTCGGGTCCGTCGGGTCCGGCGGAtccg                                                                                      >  2:4261689/1‑99 (MQ=14)
                        cgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcgg                                                                                      >  2:115367/5‑101 (MQ=14)
                          tggggtCCGTGGGGTCGGTCGGGTCCGTGGGGTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcggtc                                                                                    <  1:3201062/96‑1 (MQ=11)
                          tcggATCGGTCGGATCCTTCGGCTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcggtc                                                                                    <  2:612909/101‑1 (MQ=17)
                          tcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGCTCGGTCGGGTCCGTCGGATCGGTCGGAtccgtg                                                                                    >  1:4051803/1‑100 (MQ=11)
                           gggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGAAGGATCGGTCGGGTCCGTCGGGGCCGTGGGAtcggagg                                                                                   >  1:2837668/2‑98 (MQ=14)
                           cggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtccgtgg                                                                                   <  2:256242/101‑3 (MQ=11)
                           cggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtccgggg                                                                                   >  1:4288646/1‑96 (MQ=11)
                            gggtCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCGGTGGGATCGGTCGGGTCCGTGGGGGCCGTGGGGtcggtggg                                                                                  >  2:4495658/4‑101 (MQ=11)
                            ggggccggaggATCCGTCGGGTCCGTAGGATCGGTCGGATCCGAGAGAACGGTAAGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtcgg                                                                                  <  2:3866726/92‑1 (MQ=9)
                            ggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTTGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtccgtggg                                                                                  <  1:754465/101‑1 (MQ=11)
                             gATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGa                                                                                 <  1:2589870/101‑1 (MQ=11)
                                 ggtcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCg                                                                             <  1:354585/97‑1 (MQ=18)
                                 cGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGGGGGGGAGGAGGGGCGCGTCGGGTCGGTGGGGtcagtggggtggg                                                                             >  2:3234369/1‑96 (MQ=2)
                                  gtcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCgg                                                                            >  1:2207289/4‑100 (MQ=17)
                                  gTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTGGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCgg                                                                            >  2:201886/1‑100 (MQ=11)
                                  gTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGt                                                                            <  2:2816991/101‑1 (MQ=14)
                                   tcggATCCGTGGGGTCCGTGTGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTg                                                                           <  2:2755818/99‑1 (MQ=18)
                                   nggngtcggtcggGGCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTg                                                                           <  1:4103683/96‑1 (MQ=14)
                                    cggATCCGTGGGGTCCGTGGGATCGGTCGGGGCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGGTCGGGCGGATcctggg                                                                          >  2:2114730/2‑97 (MQ=17)
                                    aggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGGCCGTCGGCTCCGTGGGGTCCGTCGGGGCCGTGGGATCGGTCGGGTCCGtcg                                                                          >  2:3377988/2‑99 (MQ=11)
                                     ggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTggg                                                                         >  1:2437902/1‑101 (MQ=255)
                                     ggAGCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTcgg                                                                         <  2:1655779/101‑1 (MQ=14)
                                      gATCGGTCGGGTCCGTGGGATCCGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGa                                                                        <  2:1349737/101‑1 (MQ=17)
                                       gtcggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAt                                                                       <  2:2899891/100‑1 (MQ=14)
                                         cggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTGGGGTCCGTCGGAtcg                                                                     >  2:1040878/1‑101 (MQ=14)
                                         cggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcg                                                                     <  2:483292/101‑1 (MQ=14)
                                           gtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGGCCGTCGGAtcggt                                                                   >  1:2997382/1‑101 (MQ=14)
                                            tcggGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggtc                                                                  >  1:3364341/1‑101 (MQ=17)
                                             tggattggggggATCGGGCGGGTCCGTGGGATCGGTCGGTTCCGTGGGATCG.........GTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgg                                                        <  2:925181/92‑1 (MQ=14)
                                               ggTCCGTGGGATCCGTCGGATCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCggg                                                               <  2:4081237/101‑1 (MQ=17)
                                                atCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGt                                                              >  2:4197387/2‑101 (MQ=11)
                                                 tCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGGTCGGTCGGGTCCGTCGGATCGGTCGGGTc                                                             >  1:3916564/1‑101 (MQ=17)
                                                 tCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTc                                                             <  1:4356118/101‑1 (MQ=17)
                                                  ccGTGGGATCGGGCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTcc                                                            <  1:197149/101‑1 (MQ=11)
                                                   cGTGGGATCTGGCGGACCCGTGGGATCGGTGGGGTCCGTGGGGTCCGGGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCg                                                           <  2:3319341/101‑1 (MQ=2)
                                                    gtcggATCGGTCGGATCCGTGGGATCGGTCGGGGCCGTCGGATCCGTGGGGTCCGTGGGGTCCGTGGGGTCGGTCGGGGCCGTGGGCTCGGTGGGGGCCGt                                                          >  2:3601082/4‑101 (MQ=17)
                                                    gTGGGGTCGGTCGGGGCCGTGGGATCGGTCGGGTCCGTAGGGTCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGt                                                          <  1:1196382/101‑1 (MQ=18)
                                                     tGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCGGCGGGGTCCGTg                                                         >  2:3912083/1‑101 (MQ=14)
                                                     tGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGta                                                         >  1:611778/1‑100 (MQ=17)
                                                      gggATCGGGCGGGTCCGGGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgg                                                        <  1:4197675/101‑1 (MQ=255)
                                                      cggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGGTCGGTCGGGTCCGTCGGATCGGTCGGGTCCGGgg                                                        >  2:1968857/2‑101 (MQ=17)
                                                      cggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGGCCGTgg                                                        >  1:104119/2‑101 (MQ=17)
                                                       ggttctgtcggTTCGTTGGGATCGGTCGGGTCGCGGGGTACGCTCTGTTCCGATGGGTCGGTGGGATCGGTTGGGTCCGTGGGATCGGTCGGGTCCGTagg                                                       <  2:295481/95‑1 (MQ=2)
                                                         ttctgtccgATCCGTGGGTTCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGGGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggat                                                     <  2:4427174/97‑3 (MQ=17)
                                                         gtcggtcggATCCGGGGGAGCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggat                                                     <  2:1830019/100‑3 (MQ=14)
                                                         aTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggat                                                     >  1:1525413/1‑99 (MQ=17)
                                                         aTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggat                                                     <  2:3446796/101‑3 (MQ=14)
                                                          tcggtcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGGCGGATCGGGCGGGTCCGTgggatg                                                    >  2:2495668/1‑98 (MQ=17)
                                                            cgtgggATCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcgg                                                  <  1:1463148/97‑6 (MQ=14)
                                                             gtcggATCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGGCCGGTCGGGTCCGTaggatcggt                                                 >  1:1673454/1‑95 (MQ=14)
                                                              tcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtc                                                <  2:4149616/101‑8 (MQ=11)
                                                              tcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtc                                                <  1:4587335/101‑8 (MQ=11)
                                                              tcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGGCCGTCGGATCGGGCGGGTCCGTgggatcgggg                                                >  1:2342895/1‑94 (MQ=14)
                                                               ggggtCCGGGGGATGGGTCGGGTCGGTTGGATCGGTGGGATCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtggtgg                                               <  2:2196585/97‑1 (MQ=17)
                                                               cggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGGCCGGggggacggggg                                               >  2:3730726/1‑94 (MQ=14)
                                                                 ggtCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGa                                             >  1:3580600/3‑101 (MQ=255)
                                                                 gATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGGCGGGTCCGTgggatcgggcggg                                             >  1:3674423/1‑91 (MQ=11)
                                                                      gTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGGGGGAtcggt                                        >  1:1254336/1‑101 (MQ=255)
                                                                       tGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTTGTGGGAtcggtc                                       <  1:2522619/101‑1 (MQ=255)
                                                                             cggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTTGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCg                                 <  2:15763/101‑1 (MQ=255)
                                                                              ggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGc                                >  1:2950663/1‑101 (MQ=255)
                                                                              ggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGc                                >  2:1720676/1‑101 (MQ=255)
                                                                                 cggGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGt                             >  1:3258448/1‑101 (MQ=255)
                                                                                    gTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgc                          >  1:1347498/1‑101 (MQ=255)
                                                                                      gcGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgccg                        <  2:2266703/100‑1 (MQ=255)
                                                                                       cGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGTGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGCTCGCTGTcgctgc                       >  2:738680/1‑101 (MQ=25)
                                                                                        gTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgccgcc                      >  1:2704725/1‑101 (MQ=255)
                                                                                           ggATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCgccgccgcc                   <  1:580786/101‑1 (MQ=255)
                                                                                             aTCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCgg                 >  2:4623964/1‑101 (MQ=255)
                                                                                             aTCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCgg                 <  2:2437736/101‑1 (MQ=255)
                                                                                                         cgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAggc     >  2:3993224/1‑101 (MQ=255)
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GGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGG  >  NC_014034/221973‑222181

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG