Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 222,090 G→C 7.9% P189P (CCC→CCG)  RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034222,0900G→C 7.9% 66.9 0.0 ‑0.2425P189P (CCC→CCG) RCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (15/19):  ref base (178/213):  total (193/232)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.00e-01

Only 100 of 550 total aligned reads displayed.

TGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGACCGG  >  NC_014034/221990‑222190
                                                                                                    |                                                                                                    
tgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTGGGGTCGGGCGGAGCCGTTGGGTCCG.CGGGGCCGGgg                                                                                                     >  2:1975116/1‑101 (MQ=11)
 cgggtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTgg                                                                                                     >  1:443950/8‑101 (MQ=11)
 aggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGCCCGTCGGACCCGTGGGGGCCGTGGGGCCCGTgc                                                                                                     >  1:4550379/2‑100 (MQ=2)
  gggttggagggACCCGGGGTATCGGTCGGGCCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgg                                                                                                    <  2:1636830/92‑1 (MQ=11)
  gggtccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTggg                                                                                                    <  2:3026582/92‑1 (MQ=14)
  ggacccctgcgATCCTTGGGATTGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgg                                                                                                    <  2:654023/91‑1 (MQ=1)
   ggtccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGa                                                                                                   <  2:2896088/93‑1 (MQ=14)
   ggtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGa                                                                                                   <  2:3180634/96‑1 (MQ=11)
   ggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGCGGGa                                                                                                   >  1:2499852/6‑101 (MQ=11)
   gatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTGGGATCCGTAGGATCGGTCGAGTAAGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGa                                                                                                   >  1:3985769/1‑101 (MQ=11)
   gatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTgggg                                                                                                   <  2:2485132/101‑2 (MQ=11)
    gtcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAt                                                                                                  <  1:1492107/100‑1 (MQ=11)
    atcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTggggt                                                                                                  >  1:2823377/1‑99 (MQ=11)
     tccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtc                                                                                                 >  1:3026450/4‑101 (MQ=11)
      ggggcggggccGTGGGATCGGTCGGGTCCGTTGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtcc                                                                                                <  1:696197/92‑2 (MQ=11)
      cggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtcc                                                                                                <  1:2560108/101‑2 (MQ=11)
       cgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtccg                                                                                               >  1:1226326/2‑99 (MQ=11)
        gtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtccgt                                                                                              <  1:160441/101‑1 (MQ=11)
        ggcggGTCCGTAGGATCAGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggt                                                                                              <  2:4189315/99‑1 (MQ=14)
         tcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGGCCGGGGGGtcggtc                                                                                             >  2:2685974/1‑101 (MQ=14)
         tcggGTCCGGGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcggtc                                                                                             <  1:4290638/101‑1 (MQ=11)
         tcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGCTCGGTCGGGTCCGTCGGATCGGTCGGAtccgtg                                                                                             >  1:4051803/1‑100 (MQ=11)
          gggATCCGGGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtccgtgg                                                                                            <  1:3564843/100‑3 (MQ=11)
          cggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtccgtgg                                                                                            <  2:256242/101‑3 (MQ=11)
          cggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtccgtgg                                                                                            >  2:3214313/1‑99 (MQ=11)
           gggtCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtcgg                                                                                           >  1:1684105/4‑101 (MQ=14)
           gggtCCGTAGGATCGGTCGGGTCCGTCGGACAGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtcgg                                                                                           >  1:4149904/4‑101 (MQ=14)
           ggggccggaggATCCGTCGGGTCCGTAGGATCGGTCGGATCCGAGAGAACGGTAAGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtcgg                                                                                           <  2:3866726/92‑1 (MQ=9)
           ggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGCGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGGTCGGGCGGGtctgtcgg                                                                                           >  1:295513/1‑101 (MQ=11)
            ggtCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcgggcggg                                                                                          >  1:506346/3‑100 (MQ=14)
             aTCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGag                                                                                         >  2:928798/1‑100 (MQ=11)
              gccGTGGGGTCCGGCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATc                                                                                        <  2:4489470/100‑1 (MQ=11)
                ggtcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCg                                                                                      >  2:1417577/5‑101 (MQ=17)
                 gTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGt                                                                                     <  2:2816991/101‑1 (MQ=14)
                  tcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCCTCGGGCCCGTGGGGtccggcgggcacgcg                                                                                    >  2:1782207/3‑94 (MQ=14)
                  tGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGATCCGTGGGGTCGGTGGGACCGGTCGGGTCCGTg                                                                                    >  2:4247650/1‑101 (MQ=11)
                   gggATCGGTCGGGTCGGTGGGATCGGTCGGATCGGTGGGATCGGTGGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTgg                                                                                   <  1:908217/101‑1 (MQ=14)
                   aggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCCGTGGGGTCCGTGGGGTCCGTGGGATCGGTGGGGTCGGTgg                                                                                   >  1:1012627/2‑101 (MQ=14)
                    ggATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGGggg                                                                                  >  2:3128113/1‑101 (MQ=14)
                    ggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTcgg                                                                                  >  1:4378585/1‑101 (MQ=14)
                      aTCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAt                                                                                >  1:1967427/1‑101 (MQ=255)
                       tccttggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtc                                                                               <  1:328885/95‑1 (MQ=255)
                       tccgtggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGGTCGGTCGGATCCGTCGGAtg                                                                               >  1:241886/7‑100 (MQ=17)
                       gcggtcggGTCCGGCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtc                                                                               <  2:1910660/100‑1 (MQ=14)
                        cggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTGGGGTCCGTCGGAtcg                                                                              >  2:1040878/1‑101 (MQ=14)
                        cggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcg                                                                              <  2:483292/101‑1 (MQ=14)
                        cggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcg                                                                              >  1:3196325/1‑101 (MQ=14)
                        ccgtggggTCCGTGGGATCGGTCGGGCCCGTAGGATCGTTCGGGTCCGTAGGAACGGTCGGATGCGTAGGGACCGTGGGAACGGTCGGAGCCGAGGGAtcg                                                                              >  2:859665/6‑101 (MQ=12)
                        cagtcggGTCAGTAGGATCGGTCAGATCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcg                                                                              <  1:3115437/99‑1 (MQ=17)
                          gtggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggt                                                                            >  2:3287235/4‑101 (MQ=255)
                           tcggGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggtc                                                                           <  1:3236324/101‑1 (MQ=17)
                           tcggGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggtc                                                                           >  1:3435385/1‑101 (MQ=17)
                           tcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAgcggtg                                                                           >  1:2175184/1‑100 (MQ=14)
                             gggTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTTGGGTCCGTCGGAtcggtcgg                                                                         >  2:125608/1‑101 (MQ=17)
                             gggTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGGCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGCCCGCCGGAtcggtccg                                                                         >  1:1075131/1‑99 (MQ=14)
                              ggTCCGTGGGATCCGTCGGATCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCggg                                                                        <  2:4081237/101‑1 (MQ=17)
                                tCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGGTCGGTCGGGTCCGTCGGATCGGTCGGGTc                                                                      >  1:3916564/1‑101 (MQ=17)
                                 ccGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTcc                                                                     <  2:527728/101‑1 (MQ=11)
                                  cGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGGTCGGTCGGGTCCGTCGGGTCCGTGGGGTCCGTCGGGTCGGTGGGATCTGCTGGGTCCg                                                                    >  2:1544072/1‑101 (MQ=11)
                                    tcggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGCCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTg                                                                  >  2:586547/3‑101 (MQ=18)
                                    tGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCGGCGGGGTCCGTg                                                                  >  2:3912083/1‑101 (MQ=14)
                                    tGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGta                                                                  >  1:611778/1‑100 (MQ=17)
                                     gggATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgg                                                                 <  1:73777/101‑1 (MQ=255)
                                      ggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGACCGGTGGGGTCCGTGGGACCGGTCGGGTCGCTGggggcgcggcg                                                                >  2:3356394/1‑93 (MQ=11)
                                      ggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTggg                                                                <  2:2764530/101‑1 (MQ=17)
                                        ttctgtccgATCCGTGGGTTCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGGGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggat                                                              <  2:4427174/97‑3 (MQ=17)
                                        aTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggat                                                              <  2:3446796/101‑3 (MQ=14)
                                         tcggtcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatc                                                             >  1:2707354/1‑98 (MQ=14)
                                          cggtcggGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtg                                                            >  1:3619714/1‑101 (MQ=255)
                                             tcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtc                                                         <  2:1183427/101‑8 (MQ=11)
                                             tcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtc                                                         <  2:1683994/101‑8 (MQ=11)
                                             tcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtc                                                         <  2:4149616/101‑8 (MQ=11)
                                             tcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtc                                                         <  1:4587335/101‑8 (MQ=11)
                                             tcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGGCCGTCGGATCGGGCGGGTCCGTgggatcgggg                                                         >  1:2342895/1‑94 (MQ=14)
                                             gcggATCCGTGGGATCGGTCGGGTCCGGGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcggtc                                                         <  2:2034848/100‑8 (MQ=14)
                                              cggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGGCCGGggggacggggg                                                        >  2:3730726/1‑94 (MQ=14)
                                               gggtCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGGggg                                                       >  1:2840649/4‑101 (MQ=255)
                                                ggtCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGGGGGa                                                      >  1:2094348/3‑101 (MQ=255)
                                                   ccGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcg                                                   >  1:3775256/1‑101 (MQ=255)
                                                    cGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcgg                                                  <  2:2307456/101‑1 (MQ=255)
                                                      tGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcggtc                                                >  1:4227725/1‑101 (MQ=255)
                                                             ggtcggGTCCGTAGGATCGGTTGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGc                                         >  1:2649478/1‑101 (MQ=255)
                                                                    tCCGTAGGGTCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgcc                                  <  1:1391020/101‑1 (MQ=255)
                                                                    gccGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgcc                                  <  1:2882624/100‑1 (MQ=255)
                                                                     ccGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgccg                                 >  2:2438220/1‑101 (MQ=255)
                                                                      cGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGTGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGCTCGCTGTcgctgc                                >  2:738680/1‑101 (MQ=25)
                                                                      cGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgccgc                                >  1:1279013/1‑101 (MQ=255)
                                                                       gTAGGAGCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGCGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgccgcc                               <  2:2380150/101‑1 (MQ=255)
                                                                          ggATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCgccgccgcc                            >  1:3676486/1‑101 (MQ=255)
                                                                               ggtcggGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACa                       >  1:1991224/1‑101 (MQ=255)
                                                                                   gggtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCTCTGTCGCCGCCGCCGGACAGAcc                   <  2:357315/101‑1 (MQ=255)
                                                                                   gggtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGAcc                   >  1:1558062/1‑101 (MQ=255)
                                                                                     gtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCa                 >  1:564015/1‑101 (MQ=255)
                                                                                      tccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAg                >  1:853423/1‑101 (MQ=255)
                                                                                        cgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAggc              >  2:3993224/1‑101 (MQ=255)
                                                                                               tctgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAg       <  1:1756189/98‑1 (MQ=255)
                                                                                                ccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGa      <  2:2207139/101‑1 (MQ=255)
                                                                                                ccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGa      >  2:459768/1‑101 (MQ=255)
                                                                                                  gtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGAcc    <  2:216133/101‑1 (MQ=255)
                                                                                                    gggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGACCgg  <  2:2950434/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
TGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGACCGG  >  NC_014034/221990‑222190

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG