Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,316 A→C 7.0% intergenic (‑112/‑193) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3160A→C 7.0% 37.0 0.0 ‑0.4228intergenic (‑112/‑193)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (7/9):  ref base (98/114):  total (105/123)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.39e-01

Only 100 of 240 total aligned reads displayed.

CATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.C.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGAC  >  NC_014034/2249217‑2249414
                                                                                                      |                                                                                                           
 aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAg                                                                                                            >  2:2366087/1‑101 (MQ=255)
 aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAg                                                                                                            >  1:4412041/1‑101 (MQ=255)
   tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                                          <  2:4082997/101‑1 (MQ=255)
    ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAggaa                                                                                                         <  1:2635272/101‑3 (MQ=255)
    ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCa                                                                                                         <  2:1008178/101‑1 (MQ=255)
    ggTGTCGTTGAGTTCCGCAGATTCCGCAGCTTGGACGGCCGGGGCAAGGAGGGGGCTCTC.CACCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGCCa                                                                                                         <  2:708838/101‑1 (MQ=255)
     gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCaa                                                                                                        <  2:4410874/101‑1 (MQ=255)
      tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAg                                                                                                       <  2:827179/101‑1 (MQ=255)
      tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAg                                                                                                       >  2:2975748/1‑101 (MQ=255)
       gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGa                                                                                                      <  1:550587/101‑1 (MQ=255)
        ttCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGAt                                                                                                     >  2:1641282/1‑101 (MQ=255)
        ttCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGAt                                                                                                     >  1:839616/1‑101 (MQ=255)
         tCTTGAGATGCGCAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                                    >  1:3404217/1‑101 (MQ=255)
         tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                                    <  1:1193317/101‑1 (MQ=255)
         tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGGGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                                    <  1:792773/101‑1 (MQ=255)
          cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                   <  2:1715527/101‑1 (MQ=255)
            tGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...g                                                                                              >  2:1811288/1‑101 (MQ=255)
            tGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...g                                                                                              <  2:2584937/101‑1 (MQ=255)
                  gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCCGGATCTTTGAGGCAAGATGAA...AGCG..GGc                                                                                      >  2:2031148/1‑101 (MQ=37)
                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                  <  1:318676/101‑1 (MQ=255)
                       gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCa                                                                                 <  1:3143737/101‑1 (MQ=255)
                       gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCa                                                                                 <  1:1136761/101‑1 (MQ=255)
                         tcccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGTAAGATGAA...GGGG..GGCTTTCATc                                                                               <  2:4171107/99‑1 (MQ=255)
                         tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATc                                                                               >  2:2191304/1‑101 (MQ=255)
                           ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCtt                                                                             <  2:3582004/101‑1 (MQ=255)
                            cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTg                                                                            >  2:2115430/1‑101 (MQ=255)
                             gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGc                                                                           >  1:4469196/1‑101 (MQ=255)
                               aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                         >  2:3627767/1‑101 (MQ=255)
                               aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGAAGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                         >  2:4032114/1‑101 (MQ=38)
                                gCTTGGGCACGCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGa                                                                        <  2:892112/101‑1 (MQ=255)
                                   tGGGCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                     >  1:4235150/1‑101 (MQ=255)
                                    gggCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                    >  2:3475674/1‑101 (MQ=255)
                                    gggCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTAAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                    >  1:3247440/1‑101 (MQ=255)
                                      gCACCCGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAatat                                                                  <  1:2931214/101‑1 (MQ=255)
                                         cccGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                               <  1:1649529/101‑1 (MQ=255)
                                         cccGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                               <  1:2947059/101‑1 (MQ=255)
                                          ccGGGGCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                              >  1:3734220/1‑101 (MQ=255)
                                            ggggCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggg                                                            >  1:4362966/1‑101 (MQ=255)
                                            ggggCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggg                                                            >  2:2434816/1‑101 (MQ=255)
                                              ggCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggg                                                          <  2:780584/101‑1 (MQ=255)
                                               gCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggg                                                         >  1:1395678/1‑101 (MQ=255)
                                               gCAAGAAGGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGAAATTTGAGGAAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAGATATCCCgggggg                                                         >  1:2440314/1‑101 (MQ=38)
                                                 aagaagGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                       <  1:1265535/101‑1 (MQ=255)
                                                 aagaagGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                       >  1:1524409/1‑101 (MQ=255)
                                                  agaagGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                      >  1:4112103/1‑101 (MQ=255)
                                                  agaagGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                      <  1:1215774/101‑1 (MQ=255)
                                                    aagGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                   <  1:191509/101‑1 (MQ=255)
                                                     agGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                  <  1:3048209/101‑1 (MQ=255)
                                                     agGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                  <  2:1962713/101‑1 (MQ=255)
                                                     agGGGGCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCAAAAATATCCCGGGGGGTGAA.Tg                                                  <  1:1580504/101‑1 (MQ=255)
                                                      gggggCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tgg                                                 >  1:984071/1‑101 (MQ=255)
                                                         ggCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                              <  1:2191454/101‑1 (MQ=255)
                                                         ggCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                              >  1:2748308/1‑101 (MQ=255)
                                                         ggCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGgcgc                                              >  1:2461494/1‑101 (MQ=255)
                                                          gCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                             <  2:262919/101‑1 (MQ=255)
                                                          gCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                             >  1:1977196/1‑101 (MQ=255)
                                                          gCTCTGCC.CCCGTCCCGTGCCGGGACT.CCCCCGAGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                             <  1:4045652/101‑1 (MQ=255)
                                                           ctctGCC.CCCGTCCCGTNCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                          <  2:25262/101‑1 (MQ=255)
                                                             ctgcc.cccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                        <  2:3403963/97‑1 (MQ=255)
                                                              tgcc.cccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCa                                       <  1:921768/98‑1 (MQ=255)
                                                               gcc.cccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                      >  2:3371068/3‑101 (MQ=255)
                                                               gcc.cccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                      <  2:1109557/99‑1 (MQ=255)
                                                                cc.cccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCaga                                     >  1:649210/2‑101 (MQ=255)
                                                                     cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                 >  1:493782/1‑101 (MQ=255)
                                                                      gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCGGAggggg                                <  2:1448065/101‑1 (MQ=255)
                                                                      gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                >  1:3628503/1‑101 (MQ=255)
                                                                       tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCTTCTTGCCGAAAAAATCCCGGGGGGGGAA.GGGGCCA..GCCAGAgggggg                               >  2:4001927/1‑101 (MQ=37)
                                                                       tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                               >  1:3661675/1‑101 (MQ=255)
                                                                        cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                              <  1:301943/101‑1 (MQ=255)
                                                                        cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAggggggg                              >  1:351778/1‑100 (MQ=255)
                                                                         ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGcg                             >  2:943842/1‑100 (MQ=255)
                                                                         ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                             <  2:3234555/101‑1 (MQ=255)
                                                                         ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                             <  2:3733945/101‑1 (MQ=255)
                                                                         ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                             <  2:156629/101‑1 (MQ=255)
                                                                          cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                            <  2:454360/101‑1 (MQ=255)
                                                                          cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                            <  1:2241603/101‑1 (MQ=255)
                                                                           gtGCCGGGACT.CCCCTGCGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                           <  1:3869997/101‑1 (MQ=255)
                                                                           gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                           >  1:3345788/1‑101 (MQ=255)
                                                                           gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                               >  2:143041/7‑101 (MQ=18)
                                                                           gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                               >  2:2807528/7‑101 (MQ=18)
                                                                             gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgcg.c                        >  2:594752/1‑101 (MQ=255)
                                                                              ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc                       <  2:4312041/101‑1 (MQ=255)
                                                                               ccgggctcccccaGGGATATTTGAGGCAAGATGAG...GGGG..GGCTTTCATCTTGACGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc                       <  1:739917/93‑1 (MQ=25)
                                                                               cGGGACG.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc                      <  1:3475932/101‑1 (MQ=255)
                                                                                gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccc                     >  1:3856632/1‑101 (MQ=255)
                                                                                gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccc                     >  2:2321797/1‑101 (MQ=255)
                                                                                gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccc                     <  2:4247913/101‑1 (MQ=255)
                                                                                cggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAgcg                          <  1:2545834/100‑1 (MQ=18)
                                                                                   aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccct                  >  1:3062505/1‑99 (MQ=255)
                                                                                   aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGACGGGGGCGGCG.cccccct                  >  2:3064665/1‑99 (MQ=255)
                                                                                       cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttcc               >  2:1358030/1‑96 (MQ=255)
                                                                                         cccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCCtgc                 >  2:1905000/1‑99 (MQ=0)
                                                                                           cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCgcggg            >  1:1804492/1‑101 (MQ=255)
                                                                                            gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCgcgggc           >  1:4656833/1‑101 (MQ=255)
                                                                                             ggATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCCtgccccg             <  2:3054626/101‑7 (MQ=0)
                                                                                               atatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGggcggc        <  2:1624353/101‑1 (MQ=255)
                                                                                                tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGcc       >  2:4603816/1‑101 (MQ=255)
                                                                                                  tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCcgc     <  2:638396/101‑1 (MQ=255)
                                                                                                   ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCcgcg    >  2:3916653/1‑101 (MQ=255)
                                                                                                    tGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGa   <  2:3821064/101‑1 (MQ=255)
                                                                                                      |                                                                                                           
CATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.C.CCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGAC  >  NC_014034/2249217‑2249414

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG