Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,347 G→C 7.9% intergenic (‑143/‑162) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3470G→C 7.9% 34.0 ‑0.8 ‑0.9212intergenic (‑143/‑162)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (7/10):  ref base (102/92):  total (109/103)
Fisher's exact test strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.19e-01

Only 100 of 231 total aligned reads displayed.

AGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAT  >  NC_014034/2249248‑2249446
                                                                                                            |                                                                                                        
aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCg                                                                                                          >  2:3627767/1‑101 (MQ=255)
aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCg                                                                                                          >  1:819492/1‑101 (MQ=255)
aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGAAATTTGAGGCAAGAGGAA...GGGG..G..GGTTTAATCTTGCCg                                                                                                          >  1:2068909/1‑101 (MQ=255)
 gCTTGGGCACGCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGa                                                                                                         <  2:892112/101‑1 (MQ=255)
 gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGa                                                                                                         <  2:4411749/101‑1 (MQ=255)
  cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGaa                                                                                                        >  2:523173/1‑101 (MQ=255)
    tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGaaaa                                                                                                      <  2:1483432/101‑1 (MQ=255)
     gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAat                                                                                                     >  2:3475674/1‑101 (MQ=255)
         aCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATcc                                                                                                 <  2:2521341/101‑1 (MQ=255)
          cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATccc                                                                                                <  1:2947059/101‑1 (MQ=255)
             ggggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCggg                                                                                             >  2:2434816/1‑101 (MQ=255)
             ggggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCggg                                                                                             >  2:6728/1‑101 (MQ=255)
                  aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                                        <  1:540217/101‑1 (MQ=255)
                  aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                                        <  1:1265535/101‑1 (MQ=255)
                  aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                                        >  1:1524409/1‑101 (MQ=255)
                  aagaagGGGCCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                                        <  2:261725/101‑1 (MQ=255)
                   agaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGTTATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGGGa                                                                                       >  1:973071/1‑101 (MQ=38)
                   agaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                                       <  1:1400873/101‑1 (MQ=255)
                   agaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                                       <  2:3774895/101‑1 (MQ=255)
                    gaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGACAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                                      <  1:3105528/101‑1 (MQ=255)
                      agtgggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                                   >  1:2665358/4‑101 (MQ=255)
                      agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCAAAAATATCCCGGGGGGTGAA.Tg                                                                                   <  1:1580504/101‑1 (MQ=255)
                        ggggCTCTGCCCCCGTCCCGTGCCGGGCCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                                 <  2:2068769/101‑1 (MQ=255)
                          ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                                               <  1:2191454/101‑1 (MQ=255)
                          ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGgcgc                                                                               >  1:2461494/1‑101 (MQ=255)
                           gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                              <  2:262919/101‑1 (MQ=255)
                           gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                              >  2:821901/1‑101 (MQ=255)
                           gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGAGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                              <  1:4045652/101‑1 (MQ=255)
                              ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                         <  2:3403963/97‑1 (MQ=255)
                               tgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCa                                                                        <  1:921768/98‑1 (MQ=255)
                                gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                       <  2:1109557/99‑1 (MQ=255)
                                  ccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCagag                                                                     >  1:3318288/1‑101 (MQ=255)
                                   cccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgg                                                                    <  2:2348383/101‑1 (MQ=255)
                                    ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAggg                                                                   >  1:4304189/1‑101 (MQ=255)
                                     cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                                  >  1:493782/1‑101 (MQ=255)
                                       tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                                >  1:3661675/1‑101 (MQ=255)
                                       tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                                >  1:2773954/1‑101 (MQ=255)
                                        cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                               <  1:1201492/101‑1 (MQ=255)
                                        cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                               <  1:3756107/101‑1 (MQ=255)
                                        cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                               <  1:301943/101‑1 (MQ=255)
                                          cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                             <  2:454360/101‑1 (MQ=255)
                                          cgtCCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...TGGG..C..GCTTTCATCTTCCCGAAAATATCCCGGGGGGTGAA.TGGCCCA..GCCAGAGGGGGGCAg                                                             <  2:3852007/101‑1 (MQ=25)
                                           gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..G..CCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                                >  2:143041/7‑101 (MQ=18)
                                           gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..G..CCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                                >  2:401988/7‑101 (MQ=18)
                                           gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..G..CCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                                <  1:2974769/95‑1 (MQ=18)
                                            caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..G..CCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGc                                                               <  2:4058991/96‑1 (MQ=18)
                                             gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.cg.c                                                        >  2:594752/1‑101 (MQ=255)
                                             gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.cg.c                                                        >  1:3413164/1‑101 (MQ=255)
                                              ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc                                                       <  2:4312041/101‑1 (MQ=255)
                                               gcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..G..CCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAg.c                                                           <  2:1690262/99‑1 (MQ=18)
                                               cGGGACG.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccc                                                      <  1:3475932/101‑1 (MQ=255)
                                                gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cccc                                                     >  1:3856632/1‑101 (MQ=255)
                                                gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cccc                                                     >  1:4100865/1‑101 (MQ=255)
                                                cggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..G..CCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAg.cg                                                          <  1:2545834/100‑1 (MQ=18)
                                                   aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cccccct                                                  >  1:3062505/1‑99 (MQ=255)
                                                   aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGACGGGGGCGG.CG.cccccct                                                  >  2:3064665/1‑99 (MQ=255)
                                                     t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttc                                                >  2:157834/1‑97 (MQ=255)
                                                     t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttc                                                <  1:1780179/101‑5 (MQ=255)
                                                     t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttc                                                <  2:741661/101‑5 (MQ=255)
                                                       cccccGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttcc                                               >  2:1358030/1‑96 (MQ=255)
                                                       cccccGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGGGCA..GCCAGAGGGGGGCAG.CG.ccccccttcg                                               >  2:3336467/1‑96 (MQ=255)
                                                        ccccGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTcgc                                               <  2:1977061/101‑1 (MQ=255)
                                                         cccGGGATATTTGCGGCAAGATGAA...GGGGCAT..GATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.CCCCCtgc                                                 >  2:1905000/1‑99 (MQ=0)
                                                         cccGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTcgcg                                              >  2:4227192/1‑101 (MQ=255)
                                                           cGGGATATTTGCGGCAAGATGAA...GGGGCAT..GATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.CCCCCtgccc                                               >  2:3971647/1‑97 (MQ=0)
                                                           cGGGATATTTGCGGCAAGATGAA...GGGG..CATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.CCCCCtgccc                                               >  1:3361751/1‑97 (MQ=0)
                                                           cGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCgcggg                                            >  1:1804492/1‑101 (MQ=255)
                                                            gggATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCgcgggc                                           >  1:3520456/1‑101 (MQ=255)
                                                            gggATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCgcgggc                                           >  1:4656833/1‑101 (MQ=255)
                                                             ggATATTTGCGGCAAGATGAA...GGGGCAT..GATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.CCCCCtgccccg                                             <  2:3054626/101‑7 (MQ=0)
                                                                 atTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCcg                                      <  2:227603/101‑1 (MQ=255)
                                                                 atTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCcg                                      >  1:3942320/1‑101 (MQ=255)
                                                                 atTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCcg                                      >  1:4445668/1‑101 (MQ=255)
                                                                     gAGACAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAc                                  <  2:4170505/101‑1 (MQ=255)
                                                                       ggCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgc                                <  1:1910438/101‑1 (MQ=255)
                                                                           aGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGCCgcgcgc                            >  1:4456455/1‑101 (MQ=255)
                                                                           aGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACgcgcgc                            <  1:6732/101‑1 (MQ=255)
                                                                               gAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                        <  1:4265493/101‑1 (MQ=255)
                                                                                      ggg..g..gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                    >  1:124686/1‑101 (MQ=255)
                                                                                      ggg..g..gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                    >  1:3794724/1‑101 (MQ=255)
                                                                                      ggg..g..gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                    <  2:2208392/101‑1 (MQ=255)
                                                                                      ggg..g..gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                    >  1:200484/1‑101 (MQ=255)
                                                                                                tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg              >  2:112372/1‑101 (MQ=255)
                                                                                                tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg              <  1:321237/101‑1 (MQ=255)
                                                                                                tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg              <  1:4387094/101‑1 (MQ=255)
                                                                                                 ttCATCATGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGc             <  1:768392/101‑1 (MQ=255)
                                                                                                   cATCTTGCCGAAAATATCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc           <  1:4206859/101‑1 (MQ=255)
                                                                                                   cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc           >  2:2637993/1‑101 (MQ=255)
                                                                                                   cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc           >  1:2900076/1‑101 (MQ=255)
                                                                                                   cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc           >  1:4154009/1‑101 (MQ=255)
                                                                                                    aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGCCGTGcccc          >  1:2445144/1‑101 (MQ=255)
                                                                                                      cTTGCGGAAAATATCCGGGGGGGTGAA.GGGCGCA..GCCGGGGGGGGGCAGCCC.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt        <  1:997103/101‑1 (MQ=255)
                                                                                                      cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt        >  2:2570841/1‑101 (MQ=255)
                                                                                                       ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt       <  1:3628035/101‑1 (MQ=255)
                                                                                                        tGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAAAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGACGCAACAGCTCGAGCCCCcttt      >  2:3385557/1‑100 (MQ=25)
                                                                                                           cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa   <  2:3694959/101‑1 (MQ=255)
                                                                                                           cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa   >  1:1066059/1‑101 (MQ=255)
                                                                                                            gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt  >  2:2994249/1‑101 (MQ=255)
                                                                                                            gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt  >  1:682579/1‑101 (MQ=255)
                                                                                                            gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt  <  1:2321959/101‑1 (MQ=255)
                                                                                                            |                                                                                                        
AGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAT  >  NC_014034/2249248‑2249446

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG