Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,359 G→A 2.4% intergenic (‑155/‑150) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3590G→A 2.4% 5.3 ‑1.0 ‑0.6204intergenic (‑155/‑150)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (104/95):  total (108/96)
Fisher's exact test strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.30e-01

Only 100 of 228 total aligned reads displayed.

GGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCC  >  NC_014034/2249261‑2249458
                                                                                                         |                                                                                                        
 gggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggg                                                                                                        <  1:3865195/101‑1 (MQ=255)
 gggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggg                                                                                                        >  1:779414/1‑101 (MQ=255)
  ggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggg                                                                                                       <  2:780584/101‑1 (MQ=255)
   gCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggg                                                                                                      >  1:1395678/1‑101 (MQ=255)
    cAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                                                     >  1:989718/1‑101 (MQ=255)
     aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggggg                                                                                                    >  1:1649433/1‑99 (MQ=255)
     aagaagGGGCCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                                                    <  2:261725/101‑1 (MQ=255)
      agaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGTTATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGa                                                                                                   >  1:973071/1‑101 (MQ=38)
      agaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                                                   <  1:1215774/101‑1 (MQ=255)
       gaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGACAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                                                  <  1:3105528/101‑1 (MQ=255)
        aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                                                <  1:4507158/101‑1 (MQ=255)
         agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                                               <  2:839540/101‑1 (MQ=255)
         agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                                               <  2:690510/101‑1 (MQ=255)
         agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCAAAAATATCCCGGGGGGTGAA.Tg                                                                                               <  1:1580504/101‑1 (MQ=255)
          gggggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tgg                                                                                              <  1:2957826/101‑1 (MQ=255)
           ggggCTCTGCCCCCGTCCCGTGCCGGGCCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                                             <  2:2068769/101‑1 (MQ=255)
             ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                                                           >  1:2748308/1‑101 (MQ=255)
              gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                                          >  1:1977196/1‑101 (MQ=255)
              gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                                          <  2:262919/101‑1 (MQ=255)
              gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGAGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                                          <  1:4045652/101‑1 (MQ=255)
               ctctGCCCCCGTCCCGTNCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                                                                       <  2:25262/101‑1 (MQ=255)
                tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..Gc                                                                                      >  1:2466060/6‑101 (MQ=255)
                 ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                                     <  2:3403963/97‑1 (MQ=255)
                  tgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCa                                                                                    <  1:921768/98‑1 (MQ=255)
                  tgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCa                                                                                    <  1:48882/98‑1 (MQ=255)
                   gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                                   >  2:3371068/3‑101 (MQ=255)
                       ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggg                                                                               >  1:1183387/1‑101 (MQ=255)
                       ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAggg                                                                               >  1:4304189/1‑101 (MQ=255)
                        cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                                              >  1:679210/1‑101 (MQ=255)
                         gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                                             >  1:3628503/1‑101 (MQ=255)
                           cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                           <  2:3544450/101‑1 (MQ=255)
                           cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                           >  2:4579212/1‑101 (MQ=255)
                            ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                                          <  2:3234555/101‑1 (MQ=255)
                            ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                                          <  2:3733945/101‑1 (MQ=255)
                             cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                         <  1:2241603/101‑1 (MQ=255)
                             cgtCCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...TGGG..CGCTTTCATCTTCCCGAAAATATCCCGGGGGGTGAA.TGGCCCA..GCCAGAGGGGGGCAg                                                                         <  2:3852007/101‑1 (MQ=25)
                              gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                                                        >  1:4170793/1‑101 (MQ=255)
                              gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                                                        >  1:3345788/1‑101 (MQ=255)
                              gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                                            >  2:143041/7‑101 (MQ=18)
                              gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                                            >  2:401988/7‑101 (MQ=18)
                               tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc.g                                                                      <  2:4216260/101‑1 (MQ=255)
                                 ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.cc                                                                   >  2:3461441/1‑101 (MQ=255)
                                  gcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAgc                                                                        <  2:1690262/99‑1 (MQ=18)
                                  ccgggctcccccaGGGATATTTGAGGCAAGATGAG...GGGG..GGCTTTCATCTTGACGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.cc                                                                   <  1:739917/93‑1 (MQ=25)
                                   gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.cccc                                                                 >  1:4100865/1‑101 (MQ=255)
                                   gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.cccc                                                                 <  2:4247913/101‑1 (MQ=255)
                                    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAgc.g.c                                                                    <  1:2451327/101‑1 (MQ=17)
                                     gactccccccGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAGC.C.cc                                                                   <  1:3450227/96‑1 (MQ=17)
                                      aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.cccccct                                                              >  1:3062505/1‑99 (MQ=255)
                                      aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGACGGGGGCGGC.G.cccccct                                                              >  2:3064665/1‑99 (MQ=255)
                                        t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.ccccccttc                                                            <  1:3700401/101‑5 (MQ=255)
                                        t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.ccccccttc                                                            >  2:157834/1‑97 (MQ=255)
                                        t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.ccccccttc                                                            >  2:538975/1‑97 (MQ=255)
                                          cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.ccccccttcc                                                           >  2:1358030/1‑96 (MQ=255)
                                           ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTcgc                                                           <  2:1977061/101‑1 (MQ=255)
                                            cccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGC.G.CCCCCtgc                                                             >  2:1905000/1‑99 (MQ=0)
                                            cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTcgcg                                                          <  1:1250189/101‑1 (MQ=255)
                                              cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGC.G.CCCCCtgccc                                                           >  2:3971647/1‑97 (MQ=0)
                                              cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCgcggg                                                        >  1:1804492/1‑101 (MQ=255)
                                                ggATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGC.G.CCCCCtgccccg                                                         <  2:3054626/101‑7 (MQ=0)
                                                  atatTTGCGGCAAAATGAA...GGGGCATGATTTCATCTTGCCCAAAATATCCC.GGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGC.G.CCCCCtgccccgcc                                                       <  2:1006226/101‑9 (MQ=9)
                                                    atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCcg                                                  <  2:227603/101‑1 (MQ=255)
                                                    atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCcg                                                  >  1:3942320/1‑101 (MQ=255)
                                                     tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCcgc                                                 <  2:638396/101‑1 (MQ=255)
                                                     tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCcgc                                                 <  1:3508841/101‑1 (MQ=255)
                                                       ttaGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGa                                               <  2:2773756/99‑1 (MQ=255)
                                                          ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGAcgc                                            <  1:1910438/101‑1 (MQ=255)
                                                              aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGCCgcgcgc                                        >  1:4456455/1‑101 (MQ=255)
                                                               gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgcc                                       <  1:2975977/101‑1 (MQ=255)
                                                                 tGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGTGAA.AGGCGCA..GCCAGAAGGGGGGAGCGG.CCCCCCTCGCCGGCGGCCGCGGCGCGCGCCGCa                                    >  2:4505292/1‑101 (MQ=25)
                                                                   aa...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGAGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa                                   >  1:3556136/1‑101 (MQ=255)
                                                                    a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGAAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                  <  1:4115703/101‑1 (MQ=255)
                                                                        gggg..ggCTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.GGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                                 <  1:3371318/101‑1 (MQ=37)
                                                                        gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAG.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                                 >  1:4271465/1‑101 (MQ=255)
                                                                        gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                                 <  1:1446647/101‑1 (MQ=255)
                                                                        gggg..ggCTTTCATCTTGCCGAAAATAGCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                                 <  1:4622264/101‑1 (MQ=255)
                                                                         ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                >  2:2431284/1‑101 (MQ=255)
                                                                         ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                <  2:2208392/101‑1 (MQ=255)
                                                                          gg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg                               <  2:4111817/101‑1 (MQ=255)
                                                                           g..ggCTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGt                              <  1:3767092/101‑1 (MQ=255)
                                                                                 tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                          <  1:4387094/101‑1 (MQ=255)
                                                                                 tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                          >  1:2049931/1‑101 (MQ=255)
                                                                                 tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                          <  1:321237/101‑1 (MQ=255)
                                                                                  ttCATCTTGCCGAAAATATCCCGGGTGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGGCGTGc                         >  1:2608560/1‑101 (MQ=37)
                                                                                    cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                       >  1:2855236/1‑101 (MQ=255)
                                                                                    cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCCCAACAGGTCGTGccc                       >  2:1122348/1‑101 (MQ=255)
                                                                                     aTCTTGCCGAAAATATCCCGGGGGGGGAA.TGGGGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGGCGGGcccc                      >  2:1368781/1‑101 (MQ=25)
                                                                                       cTTGGCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCATAGGGTGGCAGC.GCCCCCCTGCGCGGGCGGCCGCGACTCTCGCCGCACCAGGTCGTGCCCCtt                    <  2:2329171/101‑1 (MQ=21)
                                                                                            cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa               <  2:3694959/101‑1 (MQ=255)
                                                                                            cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa               >  1:1066059/1‑101 (MQ=255)
                                                                                             gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt              <  1:2321959/101‑1 (MQ=255)
                                                                                             gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt              >  2:2994249/1‑101 (MQ=255)
                                                                                              aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt             >  1:2161595/1‑101 (MQ=255)
                                                                                               aaaTATCGCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc            <  2:2461328/101‑1 (MQ=255)
                                                                                                aagcccgtggggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt           <  1:594813/93‑1 (MQ=38)
                                                                                                  tatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGt         <  1:523221/101‑1 (MQ=255)
                                                                                                  tatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGt         >  2:2572905/1‑101 (MQ=255)
                                                                                                  tatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGAGCCCCTTTGTCATTCTgc         >  1:3826008/1‑100 (MQ=255)
                                                                                                       cGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc    <  2:3898244/101‑1 (MQ=255)
                                                                                                         gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc  <  1:644304/101‑1 (MQ=255)
                                                                                                         |                                                                                                        
GGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCC  >  NC_014034/2249261‑2249458

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG