Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,373 C→A 9.2% intergenic (‑169/‑136) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3730C→A 9.2% 35.9 ‑1.2 ‑1.6184intergenic (‑169/‑136)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (7/10):  ref base (92/75):  total (99/85)
Fisher's exact test strand distribution p-value = 3.14e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.29e-02

Only 100 of 205 total aligned reads displayed.

GGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGG  >  NC_014034/2249274‑2249472
                                                                                                           |                                                                                                       
ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                                                                         >  1:2748308/1‑101 (MQ=255)
 gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                                                        >  1:1977196/1‑101 (MQ=255)
 gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                                                        <  2:2587633/101‑1 (MQ=255)
 gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                                                        <  2:262919/101‑1 (MQ=255)
 gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                                                        <  1:4451019/101‑1 (MQ=255)
 gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGAGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                                                        <  1:4045652/101‑1 (MQ=255)
  ctctGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                                                                                     <  1:18244/101‑1 (MQ=255)
   tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..Gc                                                                                                    >  1:2466060/6‑101 (MQ=255)
    ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                                                   <  2:3403963/97‑1 (MQ=255)
     tgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCa                                                                                                  <  1:48882/98‑1 (MQ=255)
       cccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCaga                                                                                                >  1:649210/2‑101 (MQ=255)
          ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggg                                                                                             >  1:1183387/1‑101 (MQ=255)
           cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                                                            >  1:679210/1‑101 (MQ=255)
            gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCGGAggggg                                                                                           <  2:1448065/101‑1 (MQ=255)
             tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                                                          >  1:3661675/1‑101 (MQ=255)
              cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                         <  1:3756107/101‑1 (MQ=255)
              cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                         <  2:3544450/101‑1 (MQ=255)
               ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                                                        <  2:3733945/101‑1 (MQ=255)
                cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                                       <  1:2241603/101‑1 (MQ=255)
                cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                                       <  2:454360/101‑1 (MQ=255)
                cgtCCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...TGGG..CGCTTTCATCTTCCCGAAAATATCCCGGGGGGTGAA.TGGCCCA..GCCAGAGGGGGGCAg                                                                                       <  2:3852007/101‑1 (MQ=25)
                 gtGCCGGGACT.CCCCTGCGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c                                                                                     <  1:3869997/101‑1 (MQ=255)
                 gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c                                                                                     >  1:4170793/1‑101 (MQ=255)
                 gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c                                                                                     >  1:3345788/1‑101 (MQ=255)
                 gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                                                          <  1:2974769/95‑1 (MQ=18)
                 gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                                                          >  2:401988/7‑101 (MQ=18)
                  tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.cg                                                                                    <  2:4216260/101‑1 (MQ=255)
                  caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGc                                                                                         <  2:4058991/96‑1 (MQ=18)
                   gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.cg.c                                                                                  >  2:594752/1‑101 (MQ=255)
                    ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc                                                                                 <  2:4312041/101‑1 (MQ=255)
                     ccgggctcccccaGGGATATTTGAGGCAAGATGAG...GGGG..GGCTTTCATCTTGACGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc                                                                                 <  1:739917/93‑1 (MQ=25)
                      gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cccc                                                                               <  2:4247913/101‑1 (MQ=255)
                       ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAg.cg.c                                                                                  <  1:2451327/101‑1 (MQ=17)
                        gactccccccGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAG.CC.cc                                                                                 <  1:3450227/96‑1 (MQ=17)
                         aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cccccct                                                                            >  1:3062505/1‑99 (MQ=255)
                           t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttc                                                                          <  2:741661/101‑5 (MQ=255)
                             cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttcc                                                                         >  2:1358030/1‑96 (MQ=255)
                             cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGGGCA..GCCAGAGGGGGGCAG.CG.ccccccttcg                                                                         >  2:3336467/1‑96 (MQ=255)
                              ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTcgc                                                                         <  2:1977061/101‑1 (MQ=255)
                                 cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.CCCCCtgccc                                                                         >  2:3971647/1‑97 (MQ=0)
                                  gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCgcgggc                                                                     >  1:1799212/1‑101 (MQ=255)
                                  gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCgcgggc                                                                     >  1:3520456/1‑101 (MQ=255)
                                   ggATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.CCCCCtgccccg                                                                       <  2:3054626/101‑7 (MQ=0)
                                     atatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGggcggc                                                                  <  2:1624353/101‑1 (MQ=255)
                                      tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGcc                                                                 >  2:4603816/1‑101 (MQ=255)
                                        tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCcgc                                                               <  1:3508841/101‑1 (MQ=255)
                                               cAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.AGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCTTGCGGCCGCGAcgcgc                                                        >  1:779436/1‑101 (MQ=25)
                                                aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgcgcc                                                       >  1:516387/1‑100 (MQ=255)
                                                 aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACgcgcgc                                                      >  1:3308320/1‑101 (MQ=255)
                                                  gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGcgcc                                                     >  2:2469247/1‑101 (MQ=255)
                                                    tGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGcgccgc                                                   >  2:1996427/1‑101 (MQ=255)
                                                     gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                                                  <  1:4265493/101‑1 (MQ=255)
                                                       a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGAAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                <  1:4115703/101‑1 (MQ=255)
                                                           gggg..ggCTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.GGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                                               <  1:3371318/101‑1 (MQ=37)
                                                           gggg..ggCTTTCATCTTGCCGAAAATAGCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                                               <  1:4622264/101‑1 (MQ=255)
                                                            ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                              <  2:2208392/101‑1 (MQ=255)
                                                            ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                              >  1:200484/1‑101 (MQ=255)
                                                            ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                              >  2:2431284/1‑101 (MQ=255)
                                                            ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                              >  1:3794724/1‑101 (MQ=255)
                                                            ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                              >  1:124686/1‑101 (MQ=255)
                                                             gg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg                                             <  2:4111817/101‑1 (MQ=255)
                                                                    tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                        >  1:2049931/1‑101 (MQ=255)
                                                                    tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                        <  1:321237/101‑1 (MQ=255)
                                                                    tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                        <  1:4387094/101‑1 (MQ=255)
                                                                     ttCATCTTGCCGAAAATATCCCGGGTGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGGCGTGc                                       >  1:2608560/1‑101 (MQ=37)
                                                                     ttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGc                                       >  1:1385125/1‑101 (MQ=255)
                                                                       cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                     >  1:2855236/1‑101 (MQ=255)
                                                                       cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                     >  2:2637993/1‑101 (MQ=255)
                                                                       cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCCCAACAGGTCGTGccc                                     >  2:1122348/1‑101 (MQ=255)
                                                                          cTTGGCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCATAGGGTGGCAG.CGCCCCCCTGCGCGGGCGGCCGCGACTCTCGCCGCACCAGGTCGTGCCCCtt                                  <  2:2329171/101‑1 (MQ=21)
                                                                          cTTGCGGAAAATATCCGGGGGGGTGAA.GGGCGCA..GCCGGGGGGGGGCAGCCC.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                  <  1:997103/101‑1 (MQ=255)
                                                                          cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                  >  2:2570841/1‑101 (MQ=255)
                                                                           ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                 <  1:3628035/101‑1 (MQ=255)
                                                                               cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                             <  2:3694959/101‑1 (MQ=255)
                                                                               cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                             >  1:1066059/1‑101 (MQ=255)
                                                                                gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                            >  2:2994249/1‑101 (MQ=255)
                                                                                gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                            <  1:2321959/101‑1 (MQ=255)
                                                                                 aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt                           >  1:2161595/1‑101 (MQ=255)
                                                                                 aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt                           >  1:3955649/1‑101 (MQ=255)
                                                                                   aagcccgtggggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                         <  1:594813/93‑1 (MQ=38)
                                                                                   aaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                         >  1:445804/1‑101 (MQ=255)
                                                                                    atatCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGCCACGCGCCGCAACAGGTCGTGCCCCTTGGTCAGTCTg                        >  2:2276934/1‑101 (MQ=37)
                                                                                     tatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGt                       >  2:3475903/1‑101 (MQ=255)
                                                                                     tatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGAGCCCCTTTGTCATTCTgc                       >  1:3826008/1‑100 (MQ=255)
                                                                                         ccGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCa                   >  1:927373/1‑101 (MQ=255)
                                                                                          cGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc                  <  2:3898244/101‑1 (MQ=255)
                                                                                          cGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGAGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc                  >  2:1807724/1‑101 (MQ=255)
                                                                                            gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                <  2:1395583/101‑1 (MQ=255)
                                                                                            gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                >  2:3150915/1‑101 (MQ=255)
                                                                                             ggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa               >  1:4292033/1‑101 (MQ=255)
                                                                                             ggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa               >  2:3183693/1‑101 (MQ=255)
                                                                                              gggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt              >  2:862886/1‑101 (MQ=255)
                                                                                               ggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATg             <  2:1147735/101‑1 (MQ=255)
                                                                                                 tGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTc           >  2:1055919/1‑101 (MQ=255)
                                                                                                  gAA.TGGCGCA..GCCAGGGGGGGGCAGCCC.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa          <  1:4066306/101‑1 (MQ=255)
                                                                                                   aa.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt         >  2:2807285/1‑101 (MQ=255)
                                                                                                      tGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa       <  2:3062261/101‑1 (MQ=255)
                                                                                                      tGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa       <  2:649149/101‑1 (MQ=255)
                                                                                                      gggCGCA..GCGAGGGGGGGGCAGCCC.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa       <  1:157848/100‑1 (MQ=255)
                                                                                                           cA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTgg  >  1:1658780/1‑101 (MQ=255)
                                                                                                           |                                                                                                       
GGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGG  >  NC_014034/2249274‑2249472

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG