Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,380 A→G 3.4% intergenic (‑176/‑129) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3800A→G 3.4% 4.8 ‑0.4 ‑0.1175intergenic (‑176/‑129)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (4/2):  ref base (94/75):  total (98/77)
Fisher's exact test strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.79e-01

Only 100 of 202 total aligned reads displayed.

CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.CCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATC  >  NC_014034/2249281‑2249479
                                                                                                            |                                                                                                        
 cccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgg                                                                                                        <  2:2348383/101‑1 (MQ=255)
  ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAggg                                                                                                       >  1:4304189/1‑101 (MQ=255)
   cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                                                                      >  1:493782/1‑101 (MQ=255)
    gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCGGAggggg                                                                                                     <  2:1448065/101‑1 (MQ=255)
     tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                                                                    >  1:3661675/1‑101 (MQ=255)
      cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                                   <  1:3756107/101‑1 (MQ=255)
      cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                                   >  2:4579212/1‑101 (MQ=255)
      cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                                   <  2:3544450/101‑1 (MQ=255)
      cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                                   <  1:1201492/101‑1 (MQ=255)
      cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                                   <  1:301943/101‑1 (MQ=255)
       ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                                                                  >  1:4291518/1‑101 (MQ=255)
       ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                                                                  <  2:3733945/101‑1 (MQ=255)
        cgtCCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...TGGG..CGCTTTCATCTTCCCGAAAATATCCCGGGGGGTGAA.TGGCCCA..GCCAGAGGGGGGCAg                                                                                                 <  2:3852007/101‑1 (MQ=25)
        cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAggggg                                                                                                     <  2:2140257/94‑1 (MQ=18)
         gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c                                                                                               >  1:3345788/1‑101 (MQ=255)
         gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c                                                                                               >  1:4170793/1‑101 (MQ=255)
         gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                                                                    >  2:2807528/7‑101 (MQ=18)
         gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                                                                    <  1:2974769/95‑1 (MQ=18)
         gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                                                                    >  2:401988/7‑101 (MQ=18)
          tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c.g                                                                                             <  2:4216260/101‑1 (MQ=255)
          caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGc                                                                                                   <  2:4058991/96‑1 (MQ=18)
           gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c.g.c                                                                                           >  1:3413164/1‑101 (MQ=255)
            ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cc                                                                                          >  2:3461441/1‑101 (MQ=255)
             cGGGACG.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccc                                                                                         <  1:3475932/101‑1 (MQ=255)
              gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccc                                                                                        >  2:2321797/1‑101 (MQ=255)
              gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccc                                                                                        >  1:3856632/1‑101 (MQ=255)
              gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccc                                                                                        <  2:4247913/101‑1 (MQ=255)
              cggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAg.c.g                                                                                             <  1:2545834/100‑1 (MQ=18)
                 aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccccct                                                                                     >  1:3062505/1‑99 (MQ=255)
                   t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccccttc                                                                                   <  2:741661/101‑5 (MQ=255)
                   t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccccttc                                                                                   >  2:538975/1‑97 (MQ=255)
                   t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccccttc                                                                                   >  2:157834/1‑97 (MQ=255)
                     cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccccttcc                                                                                  >  2:1358030/1‑96 (MQ=255)
                     cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGGGCA..GCCAGAGGGGGGCAG.C.G.ccccccttcg                                                                                  >  2:3336467/1‑96 (MQ=255)
                       cccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.C.G.CCCCC..tgc                                                                                  >  2:1905000/1‑99 (MQ=0)
                       cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTcgcg                                                                               <  1:1250189/101‑1 (MQ=255)
                         cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.C.G.CCCCC..tgccc                                                                                >  2:3971647/1‑97 (MQ=0)
                          gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCgcgggc                                                                            >  1:3520456/1‑101 (MQ=255)
                          gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCgcgggc                                                                            >  1:1799212/1‑101 (MQ=255)
                           ggATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.C.G.CCCCC..tgccccg                                                                              <  2:3054626/101‑7 (MQ=0)
                           ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCgcgggcg                                                                           >  1:2624461/1‑101 (MQ=255)
                             atatTTGCGGCAAAATGAA...GGGGCATGATTTCATCTTGCCCAAAATATCCC.GGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.C.G.CCCCC..tgccccgcc                                                                            <  2:1006226/101‑9 (MQ=9)
                               atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCcg                                                                       >  1:4445668/1‑101 (MQ=255)
                               atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCcg                                                                       <  2:227603/101‑1 (MQ=255)
                               atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCcg                                                                       >  1:3942320/1‑101 (MQ=255)
                                tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCcgc                                                                      <  1:3508841/101‑1 (MQ=255)
                                  ttaGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGa                                                                    <  2:2773756/99‑1 (MQ=255)
                                     ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGAcgc                                                                 <  1:1910438/101‑1 (MQ=255)
                                       cAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.AGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCTTGCGGCCGCGAcgcgc                                                               >  1:779436/1‑101 (MQ=25)
                                         aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACgcgcgc                                                             <  1:6732/101‑1 (MQ=255)
                                         aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACgcgcgc                                                             >  1:3308320/1‑101 (MQ=255)
                                          gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGcgcc                                                            >  2:1985429/1‑101 (MQ=255)
                                            tGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGTGAA.AGGCGCA..GCCAGAAGGGGGGAG.CGG.CCCCC..CTCGCCGGCGGCCGCGGCGCGCGCCGCa                                                         >  2:4505292/1‑101 (MQ=25)
                                             gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCa                                                         <  1:4265493/101‑1 (MQ=255)
                                               a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGAAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                       <  1:4115703/101‑1 (MQ=255)
                                                   gggg..ggCTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.GGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACa                                                      <  1:3371318/101‑1 (MQ=37)
                                                   gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACa                                                      <  1:1446647/101‑1 (MQ=255)
                                                   gggg..ggCTTTCATCTTGCCGAAAATAGCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACa                                                      <  1:4622264/101‑1 (MQ=255)
                                                    ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                                     >  1:3794724/1‑101 (MQ=255)
                                                    ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                                     >  1:200484/1‑101 (MQ=255)
                                                     gg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg                                                    <  2:4111817/101‑1 (MQ=255)
                                                            tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                               >  1:2049931/1‑101 (MQ=255)
                                                            tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                               <  1:321237/101‑1 (MQ=255)
                                                            tttCATCTTGCAGAAAATACCCGGGGGGGTGAA.GGGCGCA..GCCAGAGGGGGGCCGCC.C.CCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                               <  1:943922/101‑1 (MQ=255)
                                                               cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                            >  1:2855236/1‑101 (MQ=255)
                                                               cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCCCAACAGGTCGTGccc                                            >  2:1122348/1‑101 (MQ=255)
                                                                aTCTTGCCGAAAATATCCGGGGGGGTGAA.TGGCTCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACATGTCGTGcccc                                           <  1:4032399/101‑1 (MQ=37)
                                                                aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGCCGTGcccc                                           >  1:2445144/1‑101 (MQ=255)
                                                                  cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                         >  2:2570841/1‑101 (MQ=255)
                                                                   ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                        >  2:2635338/1‑101 (MQ=255)
                                                                   ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                        <  1:3628035/101‑1 (MQ=255)
                                                                       cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                                    <  2:3694959/101‑1 (MQ=255)
                                                                        gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                                   >  2:2994249/1‑101 (MQ=255)
                                                                        gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                                   >  1:682579/1‑101 (MQ=255)
                                                                         aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt                                  >  1:2161595/1‑101 (MQ=255)
                                                                          aaaTATCGCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                                 <  2:2461328/101‑1 (MQ=255)
                                                                           aaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                                >  1:113275/1‑101 (MQ=255)
                                                                           aaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                                >  1:445804/1‑101 (MQ=255)
                                                                             tatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGt                              <  1:523221/101‑1 (MQ=255)
                                                                                  cGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc                         <  2:3898244/101‑1 (MQ=255)
                                                                                  cGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCCCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTTGCAc                         >  1:1608715/1‑101 (MQ=38)
                                                                                  cGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGAGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc                         >  2:1807724/1‑101 (MQ=255)
                                                                                    gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                       >  2:3150915/1‑101 (MQ=255)
                                                                                    gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                       >  2:349856/1‑101 (MQ=255)
                                                                                    gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                       <  1:644304/101‑1 (MQ=255)
                                                                                    gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                       <  2:1395583/101‑1 (MQ=255)
                                                                                     ggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa                      >  2:3183693/1‑101 (MQ=255)
                                                                                      gggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt                     <  2:4291230/101‑1 (MQ=255)
                                                                                       ggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATg                    <  2:1147735/101‑1 (MQ=255)
                                                                                        gTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt                   <  1:2279293/101‑1 (MQ=255)
                                                                                          gAA.TGGCGCA..GCCAGGGGGGGGCAGCC.C.CCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa                 <  1:4066306/101‑1 (MQ=255)
                                                                                              tGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa              <  2:3062261/101‑1 (MQ=255)
                                                                                              tGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa              <  2:649149/101‑1 (MQ=255)
                                                                                                   cA..GCCAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTgg         >  1:1658780/1‑101 (MQ=255)
                                                                                                        ccggagGGGGGCAGCC.C.CCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCg      <  1:1049738/98‑1 (MQ=255)
                                                                                                        ccAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCg      >  2:612521/1‑101 (MQ=255)
                                                                                                         cAGAGGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGc     >  2:2580717/1‑101 (MQ=255)
                                                                                                          agagGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCa    >  2:897599/1‑101 (MQ=255)
                                                                                                           gagGGGGGCAG.C.G.CCCCCCTTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCa    <  2:1649322/101‑1 (MQ=255)
                                                                                                            agGGGGGCAG.C.GCCCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATc  <  1:3819061/101‑1 (MQ=255)
                                                                                                            |                                                                                                        
CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.CCCCC..TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATC  >  NC_014034/2249281‑2249479

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG