BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,254,791	G→A	7.7%	F25F (TTC→TTT)	RCAP_rcc02086 ←	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,254,791	0	G→A	7.7%	48.3 0.0 ‑0.3	259	F25F (TTC→TTT)	RCAP_rcc02086	hypothetical protein
Reads supporting (aligned to +/- strand): new base (10/10): ref base (124/114): total (134/125)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.88e-01

Only 100 of 268 total aligned reads displayed.


GCGACGCTGGCGCCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAAT  >  NC_014034/2254693‑2254891


           gcCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGt                                                                                         <  1:3636952/101‑1 (MQ=255)


           gcCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGt                                                                                         <  2:2454763/101‑1 (MQ=255)


            ccGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATACCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCatcgtc                                                                                        <  2:3951234/101‑6 (MQ=255)


             cGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCg                                                                                       >  2:4568800/1‑101 (MQ=255)


              ggACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGa                                                                                      >  1:2587011/1‑101 (MQ=255)


               gACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGaa                                                                                     <  2:4239662/101‑1 (MQ=255)


                  gCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCg                                                                                  <  2:3579525/101‑1 (MQ=255)


                  gCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCg                                                                                  <  1:1493501/101‑1 (MQ=255)


                    ccTTCAATTGGCCACCCCAAACAGGTGATCGCATGCCGCTTCCCCTTCGCTCCCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGt                                                                                >  2:3711263/1‑101 (MQ=255)


                    ccTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGCCGAAGCggc                                                                                >  1:3248022/1‑100 (MQ=255)


                      ttCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCg                                                                              <  2:947377/101‑1 (MQ=255)


                       tCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGt                                                                             >  1:2461213/1‑101 (MQ=255)


                       tCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGt                                                                             >  1:1614860/1‑101 (MQ=255)


                        cAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTGTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTa                                                                            >  2:4036174/1‑101 (MQ=255)


                         aaCTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGGCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTaa                                                                           >  1:4320599/1‑101 (MQ=255)


                         aaCTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTaa                                                                           <  1:4214020/101‑1 (MQ=255)


                          aCTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTaaa                                                                          >  1:4578518/1‑101 (MQ=255)


                            tGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGa                                                                        <  1:4645352/101‑1 (MQ=255)


                              gACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACg                                                                      <  2:2574050/101‑1 (MQ=255)


                               acacCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGt                                                                     <  2:4069409/101‑1 (MQ=255)


                               acacCCCAAACAGGTGAGCGCATGCCGTTGCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGt                                                                     <  1:2273205/101‑1 (MQ=255)


                                  ccccAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCCGTCGTAAAGACGTTTc                                                                  <  1:4076652/101‑1 (MQ=255)


                                   cccAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCg                                                                 <  2:519731/101‑1 (MQ=255)


                                    ccAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGc                                                                >  1:3335830/1‑101 (MQ=255)


                                     cAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGcc                                                               >  2:203013/1‑101 (MQ=255)


                                        aCAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGccccc                                                            <  2:1635967/101‑1 (MQ=255)


                                        aCAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGccccc                                                            >  2:3487037/1‑101 (MQ=255)


                                         cAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCa                                                           <  2:276363/101‑1 (MQ=255)


                                         cAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCa                                                           <  1:1067591/101‑1 (MQ=255)


                                             tGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCtt                                                       <  1:1795375/101‑1 (MQ=255)


                                              gATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCttt                                                      <  1:3447159/101‑1 (MQ=255)


                                               aTCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTc                                                     >  2:3023375/1‑101 (MQ=255)


                                               aTCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTc                                                     >  1:1025151/1‑101 (MQ=255)


                                                   cATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGgcg                                                 >  2:3523240/1‑101 (MQ=255)


                                                   cATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGgcg                                                 >  2:3560489/1‑101 (MQ=255)


                                                   cATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGgcg                                                 >  2:1665185/1‑101 (MQ=255)


                                                   cATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGgcg                                                 >  2:637702/1‑101 (MQ=255)


                                                    aTGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGgcgc                                                >  2:182387/1‑101 (MQ=255)


                                                      gCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCgg                                              >  2:1896289/1‑101 (MQ=255)


                                                       ccGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCggg                                             <  2:1910878/101‑1 (MQ=255)


                                                           ttCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtct                                         <  2:902669/101‑1 (MQ=255)


                                                           ttCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtct                                         >  2:2664401/1‑101 (MQ=255)


                                                           ttCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtct                                         >  1:2953802/1‑101 (MQ=255)


                                                            tCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtctc                                        >  2:3174796/1‑101 (MQ=255)


                                                             ccccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTcc                                       >  2:3163798/1‑101 (MQ=255)


                                                              cccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCca                                      <  2:3445356/101‑1 (MQ=255)


                                                               tcgatcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgag                                     <  1:1244030/92‑1 (MQ=255)


                                                               ccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcag                                     >  2:1501982/1‑101 (MQ=255)


                                                                  tCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagcag                                  <  2:157302/101‑1 (MQ=255)


                                                                  tCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagcag                                  >  1:3054912/1‑101 (MQ=255)


                                                                  atcgcgtcGCCCCCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggcc                                  >  1:1927095/7‑98 (MQ=255)


                                                                   tcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccg                                 >  2:1158892/6‑97 (MQ=255)


                                                                   tcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccg                                 <  2:2871928/96‑5 (MQ=255)


                                                                   cGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGt                                 <  1:358858/101‑1 (MQ=255)


                                                                    gCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTc                                <  1:1092135/101‑1 (MQ=255)


                                                                    gCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTc                                <  1:4120527/101‑1 (MQ=255)


                                                                    cgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgc                                <  2:779860/97‑6 (MQ=255)


                                                                    cgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgc                                <  1:2407665/97‑6 (MQ=255)


                                                                      tctcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGc                              >  1:567280/1‑101 (MQ=255)


                                                                      tctcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGc                              <  2:1844825/101‑1 (MQ=255)


                                                                      cgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgcgc                              <  1:2985551/99‑8 (MQ=255)


                                                                       gtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgcgca                             >  1:2081283/2‑93 (MQ=255)


                                                                       ctcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCa                             <  1:2759851/101‑1 (MQ=255)


                                                                       ctcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCa                             >  2:1042152/1‑101 (MQ=255)


                                                                        tcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCaa                            >  2:1691694/1‑101 (MQ=255)


                                                                         cGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAg                           <  1:4496382/101‑1 (MQ=255)


                                                                         cGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAg                           <  1:1665296/101‑1 (MQ=255)


                                                                         cGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAg                           <  1:2040309/101‑1 (MQ=255)


                                                                          gCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAgg                          <  1:1808945/101‑1 (MQ=255)


                                                                            ccTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTg                        >  2:1626065/1‑101 (MQ=255)


                                                                            ccTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTg                        <  2:2963335/101‑1 (MQ=255)


                                                                            ccTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGCCGGGAAGACGCTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTg                        >  2:330726/1‑101 (MQ=255)


                                                                               cAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCtt                     >  1:1540929/1‑101 (MQ=255)


                                                                               cAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCtt                     <  2:1025068/101‑1 (MQ=255)


                                                                               cAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGGCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCtt                     >  2:768045/1‑101 (MQ=255)


                                                                                aaTGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCttt                    <  2:436427/101‑1 (MQ=255)


                                                                                aaTGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCttt                    >  1:621398/1‑101 (MQ=255)


                                                                                 aTGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTc                   >  1:3676374/1‑101 (MQ=255)


                                                                                 aTGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTc                   >  2:2597809/1‑101 (MQ=255)


                                                                                  tGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCa                  <  2:2349360/101‑1 (MQ=255)


                                                                                  tGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCa                  <  1:1641331/101‑1 (MQ=255)


                                                                                   gggCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCag                 >  2:136007/1‑101 (MQ=255)


                                                                                    ggCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCaga                >  1:838091/1‑101 (MQ=255)


                                                                                    ggCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCaga                <  2:4531492/101‑1 (MQ=255)


                                                                                      cAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGATCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAgaga              <  1:4048175/101‑1 (MQ=255)


                                                                                       aGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGAc             <  2:3092885/101‑1 (MQ=255)


                                                                                       aGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGAc             >  1:2301970/1‑101 (MQ=255)


                                                                                        gcgcCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACg            <  2:2479276/101‑1 (MQ=255)


                                                                                        gcgcCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACg            <  2:216480/101‑1 (MQ=255)


                                                                                           ccGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCa         <  1:1189124/101‑1 (MQ=255)


                                                                                           ccGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCa         <  1:1390118/101‑1 (MQ=255)


                                                                                           ccGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCa         >  2:370672/1‑101 (MQ=255)


                                                                                           ccGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCa         <  2:219419/101‑1 (MQ=255)


                                                                                             gAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAg       <  1:1122979/101‑1 (MQ=255)


                                                                                             gAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAg       <  2:3183286/101‑1 (MQ=255)


                                                                                              aGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAgg      >  2:1536138/1‑101 (MQ=255)


                                                                                               ggCGAAGGCGTCAAGGTCGAACCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGt     >  1:2648279/1‑101 (MQ=255)


                                                                                                gCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTa    >  1:548322/1‑101 (MQ=255)


                                                                                                  gAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAAt  <  2:3786354/101‑1 (MQ=255)


                                                                                                  gAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAAt  >  1:3837969/1‑101 (MQ=255)


GCGACGCTGGCGCCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAAT  >  NC_014034/2254693‑2254891

Base quality scores:


ATCG

< 3 ≤


ATCG

< 23 ≤


ATCG

< 30 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG