Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,262,580 G→T 100% T57K (ACG→AAG)  RCAP_rcc02105 ← IS3/IS911 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,262,5800G→T100.0% 741.2 206T57K (ACG→AAG) RCAP_rcc02105IS3/IS911 family transposase
Reads supporting (aligned to +/- strand):  new base (120/86):  ref base (0/0):  total (120/86)

Only 100 of 231 total aligned reads displayed.

CTCGCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCGTCAGCTGCTTGGCATCGGACAAATCCATCCCACCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGA  >  NC_014034/2262482‑2262677
                                                                                                  |                                                                                                 
 tCGCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCa                                                                                                <  2:1547392/101‑1 (MQ=255)
 tCGCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCa                                                                                                >  1:1391846/1‑101 (MQ=255)
   gCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgc                                                                                              >  2:705629/1‑101 (MQ=255)
    ccATTGGGCTTGACCCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCCCCCGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgcc                                                                                             <  1:2827152/101‑2 (MQ=17)
    ccATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgcc                                                                                             >  1:3317223/1‑100 (MQ=255)
    ccATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgcc                                                                                             >  2:2128512/1‑100 (MQ=255)
    ccATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgcc                                                                                             >  2:3585860/1‑100 (MQ=255)
     cATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTTAgccc                                                                                            >  2:2993666/1‑99 (MQ=255)
     cATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgccg                                                                                            >  1:1750928/1‑99 (MQ=255)
     cATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgccg                                                                                            >  1:3217993/1‑99 (MQ=255)
      aTTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTGAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgccgc                                                                                           <  2:1100721/101‑1 (MQ=255)
       ttGGGCTTGAACCAGTGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgccgct                                                                                          >  2:2879909/1‑101 (MQ=255)
        tGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCtt                                                                                         <  2:4284589/101‑1 (MQ=255)
         gggCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTg                                                                                        <  2:4010542/101‑1 (MQ=255)
         gggCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTg                                                                                        <  2:1706823/101‑1 (MQ=255)
          ggCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTgg                                                                                       >  2:1874191/1‑101 (MQ=255)
             ttGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCAt                                                                                    <  2:2936950/101‑1 (MQ=255)
             ttGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCAt                                                                                    <  2:2218725/101‑1 (MQ=255)
             ttGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCAt                                                                                    >  2:1173575/1‑101 (MQ=255)
                 aCCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGa                                                                                >  1:3298393/1‑101 (MQ=255)
                  ccAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGAc                                                                               <  2:838509/101‑1 (MQ=255)
                   cAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACa                                                                              >  1:3667942/1‑101 (MQ=255)
                   cAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACa                                                                              >  1:2676171/1‑101 (MQ=255)
                     aTGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACaaa                                                                            <  1:1249460/101‑1 (MQ=255)
                      tGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAat                                                                           <  1:46334/101‑1 (MQ=255)
                       ggCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatc                                                                          <  2:4060748/101‑1 (MQ=255)
                       ggCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatc                                                                          <  1:4445479/101‑1 (MQ=255)
                        gcgcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcc                                                                         <  1:1014455/101‑1 (MQ=255)
                           ccACACTGGCTCGATCCAGCATCACATCCGCCACCAGACGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCCTCGGACAAatccatc                                                                      <  1:3937047/101‑1 (MQ=21)
                            cacaCTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatccatcc                                                                     <  1:2438571/101‑1 (MQ=255)
                             acacTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatccagatc                                                                    >  1:2086507/1‑97 (MQ=255)
                             acacTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATccc                                                                    <  2:913393/101‑1 (MQ=255)
                              cacTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATcccg                                                                   >  1:3420634/1‑100 (MQ=255)
                                cTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCccgcc                                                                 >  1:17942/1‑101 (MQ=255)
                                 tGGCTCGATCCAGCATCCCATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCccgcca                                                                <  1:1915072/101‑1 (MQ=18)
                                 tGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCccgcca                                                                >  2:227418/1‑101 (MQ=255)
                                    cTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATAc                                                             <  1:1874317/101‑1 (MQ=255)
                                      cGATCCAGCATCACACCCCCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACtt                                                           <  1:3586128/101‑1 (MQ=18)
                                       gacccAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTg                                                          <  2:1743965/98‑1 (MQ=18)
                                       gATCCAGCATCCCACCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTg                                                          <  1:3913993/101‑1 (MQ=18)
                                       gATCCAGCATCACATCCGCCACCAGGTGCTTCAGCTTGGCGTCCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTg                                                          <  2:783964/101‑1 (MQ=18)
                                       gATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTg                                                          >  2:2983816/1‑101 (MQ=255)
                                       gATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTg                                                          >  1:815111/1‑101 (MQ=255)
                                        aTCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTgg                                                         >  1:60417/1‑101 (MQ=255)
                                        aTCCAGCACCACATCCGCCCCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTgg                                                         <  1:4341755/101‑1 (MQ=18)
                                         tCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGc                                                        >  2:3921298/1‑101 (MQ=255)
                                          ccAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGcc                                                       >  1:2628037/1‑101 (MQ=255)
                                           cAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCt                                                      <  1:2928350/101‑1 (MQ=255)
                                           cAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCt                                                      >  1:507471/1‑101 (MQ=255)
                                             gCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTc                                                    >  2:4173240/1‑101 (MQ=255)
                                               aTCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAg                                                  >  2:2053195/1‑101 (MQ=255)
                                                tCACACCCGCCCCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGc                                                 <  2:3217750/101‑1 (MQ=18)
                                                 cacaTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCt                                                <  2:3714259/101‑1 (MQ=255)
                                                  ccaTCCGCCACCAGGGGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCtt                                               <  2:2534045/100‑1 (MQ=18)
                                                  acaTCCGCCCCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCtt                                               <  1:2304591/101‑1 (MQ=18)
                                                  acaTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAATTGCTTCAGCCGCTTGGCATCGGAAAAATCCATCCCGCCATACTTGGCCTTCAGCtt                                               <  2:4622739/101‑1 (MQ=17)
                                                  acaTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTGCAGCtt                                               >  1:753935/1‑101 (MQ=18)
                                                     tCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTa                                            >  2:2478771/1‑101 (MQ=255)
                                                      ccGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAg                                           >  2:2870195/1‑101 (MQ=255)
                                                         cccccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAg                                        <  2:259668/98‑1 (MQ=18)
                                                         ccaccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAg                                        <  2:452292/101‑1 (MQ=255)
                                                          ccccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAgg                                       <  1:298200/99‑1 (MQ=255)
                                                          caccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAgg                                       >  1:3795948/1‑101 (MQ=255)
                                                          caccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAgg                                       >  1:3873488/1‑101 (MQ=255)
                                                          caccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAgg                                       <  1:2251421/101‑1 (MQ=255)
                                                          caccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAgg                                       >  2:148963/1‑101 (MQ=255)
                                                            ccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTc                                     <  2:17937/101‑1 (MQ=255)
                                                            ccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTc                                     <  1:2564748/101‑1 (MQ=255)
                                                             caGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcg                                    >  2:3533235/1‑101 (MQ=255)
                                                              aGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGTCCTTCAGCTTGTAGAAGGTcgc                                   >  2:3555316/1‑101 (MQ=18)
                                                                gcgcTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCgg                                 <  2:1076493/101‑1 (MQ=255)
                                                                  gcTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCgggg                               >  1:3112111/1‑101 (MQ=255)
                                                                   cTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGc                              <  1:1480270/101‑1 (MQ=255)
                                                                   cTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGc                              >  1:1621404/1‑101 (MQ=255)
                                                                   cTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGc                              >  1:1033656/1‑101 (MQ=255)
                                                                       aGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGac                          >  1:2121165/1‑100 (MQ=255)
                                                                       aGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCCGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAg                          >  2:3827108/1‑101 (MQ=18)
                                                                        gCTTGGCTTTCTCTTCCTCAAACGGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGc                         <  2:3982772/101‑1 (MQ=21)
                                                                          ttGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGccc                       >  2:3387872/1‑101 (MQ=255)
                                                                          ttGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGccc                       <  2:1033573/101‑1 (MQ=255)
                                                                          ttGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGccc                       >  2:4443969/1‑101 (MQ=255)
                                                                            ggCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCAt                     <  2:522503/101‑1 (MQ=255)
                                                                               gTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCt                  >  2:637414/1‑101 (MQ=25)
                                                                                ttCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCtt                 >  2:815628/1‑101 (MQ=255)
                                                                                ttCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCtt                 >  1:2472748/1‑101 (MQ=37)
                                                                                 tctcTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAGTCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTc                >  2:2787983/1‑101 (MQ=25)
                                                                                 tctcTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTc                >  1:726357/1‑101 (MQ=37)
                                                                                 tctcTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTc                >  2:2640807/1‑101 (MQ=37)
                                                                                  ctctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTcc               <  1:1714277/101‑1 (MQ=25)
                                                                                    tttgcTCAAACTGCTCCAGCCGGTTGGCTCCGGACAAACCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCgg             <  2:3111867/97‑1 (MQ=21)
                                                                                    ctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCgg             <  2:2373157/101‑1 (MQ=25)
                                                                                        cTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCaaa         <  1:1193767/101‑1 (MQ=255)
                                                                                           aaaCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCt      >  2:1948151/1‑101 (MQ=255)
                                                                                           aaaCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCt      >  2:3681297/1‑101 (MQ=255)
                                                                                             aCTGCTTCAGCCGCTTGGTATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCg    <  2:2520261/101‑1 (MQ=25)
                                                                                             aCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCg    <  2:2675986/101‑1 (MQ=255)
                                                                                             aCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCg    <  1:2128662/101‑1 (MQ=255)
                                                                                              cTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCgg   <  2:3420380/101‑1 (MQ=255)
                                                                                               tGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGa  <  1:712065/101‑1 (MQ=255)
                                                                                               tGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGa  >  1:4081559/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CTCGCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCGTCAGCTGCTTGGCATCGGACAAATCCATCCCACCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGA  >  NC_014034/2262482‑2262677

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 25 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG