Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,307,862 C→G 90.5% G312A (GGC→GCC)  RCAP_rcc02140 ← extracellular ligand‑binding receptor

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,307,8620C→G90.5% 36.0 ‑0.5 0.0169G312A (GGC→GCC) RCAP_rcc02140extracellular ligand‑binding receptor
Reads supporting (aligned to +/- strand):  new base (82/71):  ref base (10/6):  total (92/77)
Fisher's exact test strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

AGATAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CGCCG.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCG  >  NC_014034/2307769‑2307952
                                                                                              |                                                                                           
aGATAGCTGGAACGGGTCGGGGTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACCAAGC.CCCGC.TTcgc                                                                                     >  2:1129166/1‑101 (MQ=255)
   tAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTcgccgc                                                                                  >  2:4604843/1‑101 (MQ=255)
     gCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGccgccg                                                                                >  1:585738/1‑101 (MQ=255)
       tGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGa                                                                              >  1:110217/1‑101 (MQ=255)
       tGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGa                                                                              >  1:2672644/1‑101 (MQ=255)
        ggAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCGGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGAt                                                                             >  1:3711941/1‑101 (MQ=255)
        ggAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGAt                                                                             >  1:4657871/1‑101 (MQ=255)
        ggAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.C.CGGCTTCGCCGCCGGAt                                                                             <  2:1766320/101‑1 (MQ=255)
         gAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATc                                                                            >  2:1256132/1‑101 (MQ=255)
         gAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.C.CGGCTTCGCCGCCGGATc                                                                            <  2:309697/101‑1 (MQ=255)
           aCGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCa                                                                          >  2:3548609/1‑101 (MQ=255)
            cgggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.C.CGGCTTCGCCGCCGGATCCAg                                                                         <  2:4005237/101‑1 (MQ=255)
            cgggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.C.CGGCTTCGCCGCCGGATCCAg                                                                         <  2:3694196/101‑1 (MQ=255)
             gggtggggtCCGCCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAgc                                                                        <  1:718091/101‑1 (MQ=255)
             gggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAgc                                                                        <  1:4238475/101‑1 (MQ=255)
              ggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAgcg                                                                       >  2:3880150/1‑101 (MQ=255)
               gtcaggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGGCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAgcgc                                                                      >  1:3471022/1‑101 (MQ=255)
                  gggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGcgccgc                                                                   >  1:1705912/1‑101 (MQ=255)
                  gggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGcgccgc                                                                   >  2:3747806/1‑101 (MQ=255)
                   ggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCcgcg                                                                  <  2:3804795/101‑1 (MQ=255)
                      tCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgc                                                               >  1:1914612/1‑101 (MQ=255)
                      tCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgc                                                               >  1:2977863/1‑101 (MQ=255)
                       cGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgcg                                                              >  1:2222029/1‑101 (MQ=255)
                       cGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgcg                                                              >  2:165106/1‑101 (MQ=255)
                        ggCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgcgc                                                             >  1:1884167/1‑101 (MQ=255)
                          ccccGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGcgccg                                                           >  1:1548230/1‑101 (MQ=255)
                             cgccgcGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCt                                                        >  1:938639/1‑101 (MQ=255)
                              gccgcGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTg                                                       <  2:1440232/101‑1 (MQ=255)
                              gccgcGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTg                                                       <  1:2922168/101‑1 (MQ=255)
                              gccgcGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTg                                                       >  1:3375807/1‑101 (MQ=255)
                               ccgcGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGa                                                      >  1:2015158/1‑101 (MQ=255)
                               ccgcGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGa                                                      >  1:1917151/1‑101 (MQ=255)
                                cgcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGAt                                                     >  1:2059733/1‑101 (MQ=255)
                                 gcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATa                                                    >  2:2300468/1‑101 (MQ=255)
                                 gcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATa                                                    <  1:3911840/101‑1 (MQ=255)
                                 gcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATa                                                    <  1:3858181/101‑1 (MQ=255)
                                 gcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATa                                                    >  1:2183794/1‑101 (MQ=255)
                                  cgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCATATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAg                                                   <  1:4015225/101‑1 (MQ=255)
                                   ggCCAGCGCCGCCAGCGCCAGGCGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgc                                                  >  1:2648109/1‑101 (MQ=255)
                                   ggCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgc                                                  >  1:403743/1‑101 (MQ=255)
                                   ggCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgc                                                  >  1:406849/1‑101 (MQ=255)
                                   ggCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgc                                                  >  2:4213826/1‑101 (MQ=255)
                                    gctagcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgcg                                                 >  1:2656296/4‑101 (MQ=255)
                                    gccagcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgcg                                                 <  1:4560969/101‑1 (MQ=255)
                                     ccagcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgcgc                                                >  2:2004370/1‑101 (MQ=255)
                                      cagcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgcgcg                                               >  2:1808275/1‑101 (MQ=255)
                                       agcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgcgcgc                                              >  1:3576866/1‑101 (MQ=255)
                                        gcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGcc                                             <  1:3413255/101‑1 (MQ=255)
                                         ccccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGcca                                            <  2:1678569/99‑1 (MQ=255)
                                           ccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGccacc                                          >  1:175079/1‑101 (MQ=255)
                                           ccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGccacc                                          <  1:4161717/101‑1 (MQ=255)
                                            cgccagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CTGTC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGccacca                                         >  2:594286/1‑101 (MQ=255)
                                              ccagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCaccacc                                       >  1:2755976/1‑101 (MQ=255)
                                               cagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCg                                      <  2:2221867/101‑1 (MQ=255)
                                                agcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCgg                                     <  2:1012123/101‑1 (MQ=255)
                                                    ccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATg                                 <  2:100954/101‑1 (MQ=255)
                                                    ccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATg                                 <  2:3855827/101‑1 (MQ=255)
                                                      agACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTc                               >  1:3054401/1‑101 (MQ=255)
                                                      acggcgcaggTCAGATATCACTCGAACAAACCGAAGCACGCC..TTCACCGCCGGATCCACCGCCGCAAGCGCCGCCGGATAGCGCGCCACCACCGGAATGTc                               <  2:720526/93‑1 (MQ=255)
                                                         cGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGgt                            <  2:4657570/101‑1 (MQ=255)
                                                          ggCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGgtg                           <  1:1195278/101‑1 (MQ=255)
                                                          ggCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGgtg                           <  1:4605144/101‑1 (MQ=255)
                                                           gCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGgtgt                          <  2:406812/101‑1 (MQ=255)
                                                            ccGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTc                         <  2:853878/101‑1 (MQ=255)
                                                             cGGTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCg                        <  2:2694368/101‑1 (MQ=255)
                                                             cGGTCAGATAGCCCTCGAAGGAGACGAAGC.C.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCg                        >  2:684970/1‑101 (MQ=255)
                                                               gTCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGGGTCGaa                      >  2:902947/1‑101 (MQ=255)
                                                                tCAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAAc                     <  2:1420781/101‑1 (MQ=255)
                                                                 cAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACg                    <  1:1413178/101‑1 (MQ=255)
                                                                 cAGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACg                    >  1:3653063/1‑101 (MQ=255)
                                                                  aGATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACgg                   <  1:1484538/101‑1 (MQ=255)
                                                                  aGATAGCCCTCGAAGGAGACGAAGC.C.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACgg                   >  1:2426173/1‑101 (MQ=255)
                                                                  aGATAGCCCTCGAAGGAGAAGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACgg                   <  1:2601773/101‑1 (MQ=255)
                                                                   gATAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGa                  <  1:274736/101‑1 (MQ=255)
                                                                   gATAGCCCTCGAAGGAGACGAAGC.C.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGa                  >  1:1139078/1‑101 (MQ=255)
                                                                    aTAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGAt                 <  1:1652083/101‑1 (MQ=255)
                                                                    aTAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGAt                 <  2:4312630/101‑1 (MQ=255)
                                                                     tAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATa                <  1:339295/101‑1 (MQ=255)
                                                                     tAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATa                <  2:3199035/101‑1 (MQ=255)
                                                                     tAGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATa                >  1:714417/1‑101 (MQ=255)
                                                                      aGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAg               <  2:644938/101‑1 (MQ=255)
                                                                      aGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAg               <  2:1165852/101‑1 (MQ=255)
                                                                      aGCCCTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAg               >  2:4449469/1‑101 (MQ=255)
                                                                        cccTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAggg             >  2:3008408/1‑101 (MQ=255)
                                                                        cccTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAggg             <  1:1256225/101‑1 (MQ=255)
                                                                        cccTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAggg             >  1:137721/1‑101 (MQ=255)
                                                                         ccTCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGc            >  2:3040077/1‑101 (MQ=255)
                                                                          cTCGAAGGAGACGAAGC.C.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCa           >  1:641512/1‑101 (MQ=255)
                                                                           tCGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCAc          <  2:2911443/101‑1 (MQ=255)
                                                                           tCGAAGGAGACGAAGC.C.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCAc          >  2:3567799/1‑101 (MQ=255)
                                                                            cGAAGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACg         <  1:1079634/101‑1 (MQ=255)
                                                                              aaGGAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGAc       <  2:4101101/101‑1 (MQ=255)
                                                                              aaGGAGACGAAGC.C.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGAc       >  2:335993/1‑101 (MQ=255)
                                                                                ggAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCt     >  2:2782469/1‑101 (MQ=255)
                                                                                ggAGACGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCt     >  1:3177368/1‑101 (MQ=255)
                                                                                 gagaCGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTg    <  2:3470764/101‑1 (MQ=255)
                                                                                  agaCGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGc   <  2:577503/101‑1 (MQ=255)
                                                                                  agaCGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGc   >  1:1950264/1‑101 (MQ=255)
                                                                                  agaCGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGc   >  1:827662/1‑101 (MQ=255)
                                                                                   gaCGAAGC.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCg  <  2:1884041/101‑1 (MQ=255)
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AGATAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGC.CGCCG.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCG  >  NC_014034/2307769‑2307952

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG