Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,330,291	C→G	100%	intergenic (+266/+46)	RCAP_rcc02161 → / ← mrcA	hypothetical protein/penicillin‑binding protein 1A

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,330,291	0	C→G	100.0%	57.7	32	intergenic (+266/+46)	RCAP_rcc02161/mrcA	hypothetical protein/penicillin‑binding protein 1A
Reads supporting (aligned to +/- strand):  new base (17/13):  ref base (0/2):  total (17/15)

CGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCCGGG..G....G.....CCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAGGGATCCGAAGGT  >  NC_014034/2330193‑2330372                                                                                                   |                                                                                             cGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccggg                                                                                            <  1:311487/101‑1 (MQ=255)   tATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..g                                                                                        >  2:2523131/1‑101 (MQ=255)     agtgggtcAAGAAAAAGCCGGGGCCCGCATGCGGGGCCCCGGATCACCGCCGGCCCGGGCGGCCTTCACCTGGGGCCCAAGAtcccgggggtccgggg..gcg                                                                                      >  2:3828701/8‑99 (MQ=255)       tGCGCCAAGAAAANGCNGGGGCGCNCNNNNNGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..gcgga                                                                                    <  1:590716/101‑5 (MQ=38)           ccAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..gcggagccc                                                                                >  1:2137467/1‑93 (MQ=255)            cAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..gcggagcccc                                                                               >  2:3976145/1‑92 (MQ=38)             aaGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGAGGCTTTCACCTGGGGCCAAAGATCCCGGGGGT.CCGGG..G....G.....cggagccccc                                                                    <  1:4136423/101‑10 (MQ=37)              aGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGT.CCGGG..G....G.....cggagcccccg                                                                   <  1:2426598/101‑11 (MQ=38)                          ggCGCGCAGGCGGGGCCCCGGATCCCCGGCGGCCCGGGCGGCTTTCCCCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGTCGCCGa                                                            <  1:3371816/101‑1 (MQ=18)                               gcATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGGCGCCCCGGCAt                                                       >  1:2255344/1‑101 (MQ=21)                                 aTGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGGCGCCGAGGccacc                                                     >  2:2723659/1‑97 (MQ=21)                                    gggggCCCCGGCCCCCGGGCGGCCCGGGCGGC.TCCCCCGGGGGGCAAAAGCCCCGGGGGGCGGGGG..G....GCGGCCCCCCCGGCGGGTCGCCGAGGCATCGCCt                                                  <  2:1905488/100‑1 (MQ=255)                                     ggggCCCCGGATCCCCGGCGGCCCGGGCGGCTTTCCCCGGGGGCCAAAGATCCGGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGTCGCCGAGGCATCGCCTc                                                 <  1:2632290/101‑1 (MQ=18)                                     ggggCCCCGGATCACCGGCGGCCGGGGCGGCTTTCCCCGGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGTCGCCGAGGCATCGCCTc                                                 <  1:1361394/101‑1 (MQ=18)                                     ggggCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGGCGGCGCGGCACCGcccc                                                 >  1:824036/1‑99 (MQ=18)                                      gggCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGGCGGCGGGGCAGCGccccc                                                >  2:3098302/1‑98 (MQ=18)                                      gggCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGGCGCCGGGGCGACGCCCCg                                                >  1:2811404/1‑101 (MQ=18)                                        gCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGGCGGCGCGGGCCCGCCTCggg                                              >  2:1370207/1‑100 (MQ=16)                                         ccccGGACCCCGGGCGGCCGGGGCGGCTTTCGCCGGGGGCCAAAGACCCGGGGGGTCGGGGG..GCGGAG.....CCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCg                                             <  1:202039/101‑1 (MQ=12)                                               aTCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGGCGGCGCGGCCTCGCCCCGGCGGAAAcc                                       >  2:3536347/1‑101 (MQ=12)                                                       cggcCCGGGGGGCTTGCACGTGGGGCCAAGGTGCCGGGGGGTCGGGGG..GCGGAG.....CCCCGGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTaa                               <  1:4313617/101‑1 (MQ=255)                                                         gcCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGGGGCCGAGGCATCGCCCCGGGGAAAAccccttttaaaa                             >  2:4086862/1‑92 (MQ=12)                                                            cGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGAcc                          <  1:4092047/101‑1 (MQ=25)                                                            cGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGGGGCCCGGGGATCGCCCCGGCGACAAACCTTATTaaaaaac                          >  2:68326/1‑97 (MQ=9)                                                            cGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGGCGCCGGGGGAGCGCCCCGGGGGAAACCCTTAGTaaaaaac                          >  1:3467715/1‑97 (MQ=9)                                                             gggCGGCTTTCCCCTGGGGCCAAAGACCCCGGGGGTCCGGGGGCG....GAC...CCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGAccg                         <  1:3098546/101‑1 (MQ=21)                                                             gggCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGGGGGCGAGGCGTCGCCTCCGCGGCATCCCCTTGGAAAAAccg                         >  1:1609374/1‑101 (MQ=9)                                                              ggcggcTTTCCCCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGAccgc                        <  2:2971730/101‑1 (MQ=21)                                                                cggcTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGGCGGCGAGGCGGCGCCCCCGCGGAAACCCCTCGTACAAAccgccc                      >  1:1623131/1‑101 (MQ=9)                                                                 gtcTTTCGCCGGGGGCCAAAGACCCGGGGGGTCGGGGG..GCGGAG.....CCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGAccgcccg                     <  2:2799186/99‑1 (MQ=16)                                                                 gtcTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGAccgcccg                     <  2:4542101/99‑1 (MQ=21)                                                                  gcTTTCCCCTGGGGCCAAAGATCCCGGGGGTCCGGGGGCG....GCC...CCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGAccgcccgc                    <  1:3843164/101‑1 (MQ=25)                                                                    tttCACCTGGGGCCAAAGATCCCGGGGGTCGGGGG..GCGGAG.....CCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGACCGCCCGCgg                  <  1:3074994/101‑1 (MQ=21)                                                                    tttCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGGCGCCGAGGGAACGCCCCGGCGAAAACCCCTTGTAAAAAACCNCCNCgg                  >  1:737228/1‑101 (MQ=9)                                                                     ttCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGGCGCCCGGGGAACGCCTCGGCGAAAACCCCTTGTAAAAACCACCCCCGGa                 >  2:1469658/1‑101 (MQ=2)                                                                          cTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAggg            <  2:3498500/101‑1 (MQ=21)                                                                                ccAAAGATCCGGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAGGGATCCGa      <  2:2870567/101‑1 (MQ=21)                                                                                ccAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAGGGATCCGa      <  2:651872/101‑1 (MQ=21)                                                                                 cAAAGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGGCGCCGGGGGGTCGCCCCGGCGCAATACCCTAGTAAAAAACGCCCCCGGACCGGGAGCCGaa     >  1:199387/1‑101 (MQ=2)                                                                                  aaaGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGGCGCCGAGGGATCGCCTCGGCGAAAACCCTTAGTAAAAACCCCCCGCGGGCAggggaccgcag    >  2:1128968/1‑93 (MQ=12)                                                                                    aGATCCCGGGGGTCCGGGG..GCGGTG.....CCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAGGGATCCGAAGGt  <  1:4255922/101‑1 (MQ=25)                                                                                    aGATCCCGGGGGTCCGGGG..GCGGAG.....CCCCCGGCGGGGCGCCGCGGGATCGCCTCGGCGCAAACCCTTAGTAAAAACCCCCCCCGGACAcggggccccaggg  >  2:1815489/1‑91 (MQ=2)                                                                                                   |                                                                                             CGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCCGGG..G....G.....CCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAGGGATCCGAAGGT  >  NC_014034/2330193‑2330372 Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG