Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,407,597 A→C 100% W83G (TGG→GGG)  nifH2 ← nitrogenase iron protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,407,5970A→C100.0% 662.0 174W83G (TGG→GGG) nifH2nitrogenase iron protein
Reads supporting (aligned to +/- strand):  new base (88/86):  ref base (0/0):  total (88/86)

Only 100 of 178 total aligned reads displayed.

CGCAGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCAGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGGATG  >  NC_014034/2407500‑2407694
                                                                                                  |                                                                                                 
 gcAGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGaaa                                                                                               >  2:2128838/1‑101 (MQ=255)
  cAGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAAc                                                                                              >  1:4563360/1‑101 (MQ=255)
  cAGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAAc                                                                                              >  1:3492812/1‑101 (MQ=255)
   aGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAAcc                                                                                             >  1:1327439/1‑101 (MQ=255)
   aGCTCTGCGAGCATGTCGACCGGGGCCGAGATGCCGCGCCCGGCGCGGCCCCCCCCCGGCCCGGG.CCTCCGCGCCTCGATGCACAGAACGCCCCCGAAAcc                                                                                             <  2:4008445/101‑1 (MQ=255)
    gCTCTTCGAGCAGACCGCCCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCCCCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCg                                                                                            <  1:2016252/101‑1 (MQ=255)
     ctctTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGa                                                                                           >  2:3601874/1‑101 (MQ=255)
      tctTCGAGCAGATCGCCCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCCCCCCCGGCCCCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAc                                                                                          <  1:483659/101‑1 (MQ=255)
      tctTCGAGCAGATCGACCGCGGCCGAGATCCCGGCCCCGGCGCAGCCCCCCCCCGGCCCCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAc                                                                                          <  1:3975507/101‑1 (MQ=255)
       ctTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcg                                                                                         >  2:1520070/1‑101 (MQ=255)
       ctTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcg                                                                                         >  2:4134813/1‑101 (MQ=255)
        ttCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcgg                                                                                        >  1:3895596/1‑101 (MQ=255)
          cggcaaGACCGCCCGCGGCGGAGTTGCCGCGCCCGGCGCAGCCCCCCCCCGGCCCCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcggcg                                                                                      <  2:1260710/96‑1 (MQ=255)
          cGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGCccgcg                                                                                      >  2:467913/1‑101 (MQ=255)
          cGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcggcg                                                                                      >  1:4361671/1‑101 (MQ=255)
          cGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcggcg                                                                                      >  2:663222/1‑101 (MQ=255)
           gAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcggcgg                                                                                     >  2:1016841/1‑101 (MQ=255)
            aGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGa                                                                                    >  2:3362491/1‑101 (MQ=255)
            aGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGa                                                                                    >  1:4395952/1‑101 (MQ=255)
              cAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCTCCCGAAACCGACGGCGGaaa                                                                                  >  2:406278/1‑101 (MQ=255)
              cAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGaaa                                                                                  >  2:2271450/1‑101 (MQ=255)
              cAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAAACCACGGCGGaaa                                                                                  >  1:1798407/1‑101 (MQ=255)
               aGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAAt                                                                                 >  2:1845976/1‑101 (MQ=255)
                 aTCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGt                                                                               >  2:2946983/1‑101 (MQ=255)
                  tCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTc                                                                              >  2:458096/1‑101 (MQ=255)
                   cgcccGCGGCCGAGACGCCGCGCCCGGCGCACCCCCCCCCCGGCCCCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCt                                                                             <  1:4329504/98‑1 (MQ=255)
                    gACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCtt                                                                            >  2:2471525/1‑101 (MQ=255)
                    gACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCtt                                                                            >  1:1395056/1‑101 (MQ=255)
                     cccGCGGCCGAGATGCCGCGCCCGGCGCAGCCCCCCCCCGGCCCCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTc                                                                           <  2:2881146/100‑1 (MQ=255)
                     cccGCGGCCGAGATGCCGCCCCCGGCGCAGCCCCCCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTc                                                                           <  2:3337716/100‑1 (MQ=255)
                      ccGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTcc                                                                          >  2:3705419/1‑101 (MQ=255)
                       cgcgGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCa                                                                         >  1:1180728/1‑101 (MQ=255)
                        gcgGCGGAGATGCCGCGCCCGGCGCACCCCCCCCCCGGCCCGGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAg                                                                        <  1:2602850/101‑1 (MQ=255)
                         cgGCCGAGCGGCCCCGCCCGCCCCAGCCCCCCCCCGGCCCCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGc                                                                       <  1:2044719/101‑1 (MQ=255)
                            ccGAGATGCCGCGCCGGGCGCAGCCCACCCCGGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTc                                                                    <  2:465933/101‑1 (MQ=255)
                               ggaTGCCCCCCCCGGCGCACCCCCCCCCCGGCCCCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGt                                                                 <  1:562839/100‑1 (MQ=255)
                                gaTCCCGCGCCCGGCGCACCCCCCCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGtt                                                                <  2:2498908/101‑1 (MQ=255)
                                 aTGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGttt                                                               >  1:2133887/1‑101 (MQ=255)
                                 aTGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGACTTCCCGCTTCGGgtt                                                               >  2:1323927/1‑101 (MQ=255)
                                 aTGCCGCCCCCGGCCCACCCCCCCCCCGGCCCCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGttt                                                               <  1:2362447/101‑1 (MQ=255)
                                  tGCCGCGCCCGGCCCACCCCACCCCCGGCCCCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTg                                                              <  1:2319263/101‑1 (MQ=255)
                                     cgcgCCCGGCGCAGCCCACCACCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGGgg                                                           >  2:1332853/1‑101 (MQ=255)
                                     cgcgCCCGGCGCACCCCACCCCCGGCCCCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCgg                                                           <  2:3262358/101‑1 (MQ=255)
                                     cgcgCCCGGCCCAGCCCCCCCCCGGCCCGGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCgg                                                           <  2:2550477/101‑1 (MQ=255)
                                         cccGGCCCACCCCCCCCCCGGCCCCGC.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCAc                                                       <  1:3618002/101‑1 (MQ=255)
                                          cgggCGCGCCCCCCCCCCGGCCCGGC.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACg                                                      <  2:1221867/99‑1 (MQ=255)
                                            ggCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGt                                                    >  1:3336609/1‑101 (MQ=255)
                                             gcgcAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTc                                                   >  2:2800575/1‑101 (MQ=255)
                                              cgcAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTcg                                                  >  1:1981053/1‑101 (MQ=255)
                                               gcAGCCCCCCCCCGGCCCCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTcgc                                                 <  2:3732114/101‑1 (MQ=255)
                                                 cccccCCCCCCGGCCCCGGCCCCGCCCGCCTCGGTGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTcgcg                                                <  1:4047952/99‑1 (MQ=255)
                                                 cccccCCCCCCGGCCCCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCgcgc                                               <  1:4349000/99‑1 (MQ=255)
                                                  gCCCCCCCCCGGCACCGG.CCCCCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCa                                              <  1:692582/101‑1 (MQ=255)
                                                  gCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCa                                              >  1:3382104/1‑101 (MQ=255)
                                                   cccCCCCCCGGCACGGC.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAg                                             <  2:314433/101‑1 (MQ=255)
                                                   cccACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAg                                             >  1:3805485/1‑101 (MQ=255)
                                                    ccccccccGGCACCGC.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAgg                                            <  1:1547811/98‑1 (MQ=255)
                                                      ccccccGGCCCCGG.CCCCCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCt                                          <  1:642705/100‑1 (MQ=255)
                                                          ccGGCCCGGC.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTcc                                      <  1:1146975/101‑1 (MQ=255)
                                                           cgcccccGC.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCa                                     <  2:2084009/96‑1 (MQ=255)
                                                            ggCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAg                                    >  2:2562991/1‑101 (MQ=255)
                                                            ggCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAg                                    >  1:1812102/1‑101 (MQ=255)
                                                             gccccGG.CCCTGCCGCCTCGATGCACAGAACGCCCCCAAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGc                                   <  1:793882/98‑1 (MQ=255)
                                                             gccccGC.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGc                                   <  2:560286/98‑1 (MQ=255)
                                                             gCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGc                                   >  1:4063727/1‑101 (MQ=255)
                                                              cccggg.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCa                                  <  2:1497009/97‑1 (MQ=255)
                                                              cACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCa                                  >  2:3274198/1‑101 (MQ=255)
                                                              cACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCa                                  >  1:3004130/1‑101 (MQ=255)
                                                               aCCGG.CCCGCCCGCCTCGACGCACAGAACGCCCCCGAAACCCACGGCGGCAATGTATTCAAGATTCGGCTTGCGAGAACGGGCGCCCAGCCTGTCCactac                                 >  1:740854/1‑97 (MQ=255)
                                                                 cGG.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCaagatcg                               >  2:142764/1‑95 (MQ=255)
                                                                  gg.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGt                              >  1:130816/1‑101 (MQ=255)
                                                                  gg.CCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGt                              >  1:3024960/1‑101 (MQ=255)
                                                                  cg.cccccccGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGt                              <  2:4351905/100‑1 (MQ=255)
                                                                   g.cccgcccGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTg                             <  2:4183857/101‑1 (MQ=255)
                                                                        ccccgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCa                         <  2:2688439/100‑1 (MQ=255)
                                                                         cccgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCCGCACGGTGGGCAg                        >  1:984283/1‑101 (MQ=255)
                                                                         cccgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAg                        >  2:773166/1‑101 (MQ=255)
                                                                           cgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGtt                      <  1:4076701/101‑1 (MQ=255)
                                                                              cTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTg                   >  2:1103912/1‑101 (MQ=255)
                                                                              cTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGCTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTg                   <  1:4642776/101‑1 (MQ=255)
                                                                               tCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGc                  >  1:94159/1‑101 (MQ=255)
                                                                                cGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGcc                 <  1:1021912/101‑1 (MQ=255)
                                                                                cGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGcc                 <  1:2215957/101‑1 (MQ=255)
                                                                                  aTGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGcccc               >  1:2097369/1‑101 (MQ=255)
                                                                                  aTGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGcccc               >  1:277533/1‑101 (MQ=255)
                                                                                  aTGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGcccc               <  1:610277/101‑1 (MQ=255)
                                                                                   tGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCccg              >  1:377102/1‑101 (MQ=255)
                                                                                     cacaGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCccgcc            >  1:4239233/1‑101 (MQ=255)
                                                                                      acaGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCccgccg           >  1:2897039/1‑101 (MQ=255)
                                                                                      acaGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCccgccg           >  1:1765315/1‑101 (MQ=255)
                                                                                       caGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCcgccgc          <  1:3844669/101‑1 (MQ=255)
                                                                                       caGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCcgccgc          <  2:1318436/101‑1 (MQ=255)
                                                                                         gAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCgcgc        >  1:1442755/1‑101 (MQ=255)
                                                                                          aaCGCCCCCGAAACCGACGGCTGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCa       <  2:4641645/101‑1 (MQ=255)
                                                                                          aaCGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCa       <  2:2133737/101‑1 (MQ=255)
                                                                                           aCGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAg      <  1:3234996/101‑1 (MQ=255)
                                                                                             gCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGGa    >  2:3010624/1‑101 (MQ=255)
                                                                                             gCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGGa    <  1:1629176/101‑1 (MQ=255)
                                                                                              ccccCGAAACCGGCGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGGAt   <  1:3685821/101‑1 (MQ=255)
                                                                                               cccCGAAACCGACGGCAGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGTAtg  <  2:2373699/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CGCAGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGG.CCCGCCCGCCTCGATGCACAGAACGCCCCAGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGGATG  >  NC_014034/2407500‑2407694

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG