Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 243,127 A→G 100% E28E (GAA→GAG mogA → molybdopterin biosynthesis mog protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034243,1270A→G100.0% 854.4 223E28E (GAA→GAGmogAmolybdopterin biosynthesis mog protein
Reads supporting (aligned to +/- strand):  new base (115/108):  ref base (0/0):  total (115/108)

Only 100 of 226 total aligned reads displayed.

GCGCGTGGCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAAGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCGGCTTCG  >  NC_014034/243029‑243224
                                                                                                  |                                                                                                 
gcgcgTGGCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGa                                                                                                 >  1:1357133/1‑101 (MQ=255)
gcgcgTGGCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGa                                                                                                 <  2:1504310/101‑1 (MQ=255)
  gcgTGGCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACt                                                                                               <  2:1961401/101‑1 (MQ=255)
  gcgTGGCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACt                                                                                               >  2:3656324/1‑101 (MQ=255)
   cgTGGCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTg                                                                                              <  1:4101257/101‑1 (MQ=255)
    gTGGCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTgg                                                                                             >  2:4450195/1‑101 (MQ=255)
    gTGGCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTgg                                                                                             <  2:3412444/101‑1 (MQ=255)
    gTGGCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTgg                                                                                             >  2:2890338/1‑101 (MQ=255)
     tGGCGCAAAAATGACGGCACGGGTTGCCATCGTCCCCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGc                                                                                            <  1:1601073/101‑1 (MQ=255)
     tGGCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGc                                                                                            <  1:1167296/101‑1 (MQ=255)
     tGGCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGc                                                                                            >  1:1207775/1‑101 (MQ=255)
      ggCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCt                                                                                           >  2:3687365/1‑101 (MQ=255)
       gcgcAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTg                                                                                          >  1:217078/1‑101 (MQ=255)
        cgcAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGa                                                                                         >  1:2418805/1‑101 (MQ=255)
         gcAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAg                                                                                        >  1:2992755/1‑101 (MQ=255)
          cAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAgg                                                                                       >  2:3474931/1‑101 (MQ=255)
          cAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAgg                                                                                       >  1:3473142/1‑101 (MQ=255)
            aaaaTGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAgggg                                                                                     <  2:262047/101‑1 (MQ=255)
             aaaTGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGgt                                                                                    <  1:4179430/101‑1 (MQ=255)
              aaTGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAgggggg                                                                                   >  1:367542/1‑99 (MQ=255)
              aaTGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGgtg                                                                                   >  2:1976747/1‑101 (MQ=255)
               aTGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGgtgt                                                                                  <  2:620526/101‑1 (MQ=255)
               aTGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGgtgt                                                                                  <  1:143568/101‑1 (MQ=255)
                 gACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTgtcg                                                                                <  1:3409120/101‑1 (MQ=255)
                  gcGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTgtcgt                                                                               <  2:2636885/100‑1 (MQ=255)
                  aCGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTgtcgt                                                                               >  2:4152800/1‑101 (MQ=255)
                      cACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACg                                                                           <  2:2149458/101‑1 (MQ=255)
                      cACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACg                                                                           <  1:3512763/101‑1 (MQ=255)
                      cACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACg                                                                           <  2:912843/101‑1 (MQ=255)
                        cGGGTTGACATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGCCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGac                                                                         <  2:2382551/101‑1 (MQ=255)
                         gggTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGaca                                                                        <  1:4049659/101‑1 (MQ=255)
                          ggTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGacac                                                                       >  1:4219833/1‑101 (MQ=255)
                            ttGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACAccc                                                                     <  1:4017295/101‑1 (MQ=255)
                             tGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACAcccc                                                                    >  1:3883183/1‑101 (MQ=255)
                               ccATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCtt                                                                  <  2:1420261/101‑1 (MQ=255)
                               ccATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCtt                                                                  <  1:2230629/101‑1 (MQ=255)
                                cATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCttt                                                                 <  1:1417915/101‑1 (MQ=255)
                                 aTCGTCGCCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTc                                                                <  2:3990452/101‑1 (MQ=255)
                                 aTCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTc                                                                <  1:195735/101‑1 (MQ=255)
                                 aTCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGGGTCGTCACGACAccccagat                                                                >  1:3300463/1‑97 (MQ=255)
                                   cGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCa                                                              <  1:424901/101‑1 (MQ=255)
                                    gTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCAt                                                             >  1:717292/1‑101 (MQ=255)
                                     tCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCAtt                                                            >  2:3181680/1‑101 (MQ=255)
                                      cACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCAttt                                                           >  2:3640938/1‑101 (MQ=255)
                                      cACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCAttt                                                           <  2:2756833/101‑1 (MQ=255)
                                       aCCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTc                                                          <  2:1282961/101‑1 (MQ=255)
                                       aCCGGCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTc                                                          <  2:3759314/101‑1 (MQ=255)
                                            ctcCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCggg                                                     >  2:552603/1‑101 (MQ=255)
                                             tcCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGGCGTCACGACACCCCTTTCCATTTCCCGGGt                                                    >  2:4242358/1‑101 (MQ=255)
                                              ccGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTg                                                   >  1:456539/1‑101 (MQ=255)
                                              ccGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGCTg                                                   >  2:192713/1‑101 (MQ=255)
                                               cGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGa                                                  <  2:583141/101‑1 (MQ=255)
                                               cGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGCGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGa                                                  <  2:4174296/101‑1 (MQ=255)
                                                gACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGat                                                 >  1:1711455/1‑101 (MQ=255)
                                                gACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGat                                                 <  2:2228314/101‑1 (MQ=255)
                                                 aCCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGGGTCGTCACGACACCCCTTTCCATTTCCCGGGTGatc                                                >  2:4580217/1‑101 (MQ=255)
                                                    gcgcCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGatcatc                                             <  2:3882898/101‑1 (MQ=255)
                                                      gcCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATccc                                           <  2:2418641/101‑1 (MQ=255)
                                                        cTCGCGCGGGGAGTATGAGGACAAGGGCGGTCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCg                                         <  2:630310/101‑1 (MQ=255)
                                                        cTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATGCCCg                                         <  1:3204309/101‑1 (MQ=255)
                                                        cTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCg                                         >  2:1199061/1‑101 (MQ=255)
                                                          cgcgcgGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGAc                                       >  1:1661345/1‑101 (MQ=255)
                                                          cgcgcgGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGAc                                       <  1:24810/101‑1 (MQ=255)
                                                          cgcgcgGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGAc                                       >  1:2645982/1‑101 (MQ=255)
                                                           gcgcgGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACg                                      >  1:2825587/1‑101 (MQ=255)
                                                            cgcgGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACgg                                     >  2:108125/1‑101 (MQ=255)
                                                            cgcgGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACgg                                     >  1:959407/1‑101 (MQ=255)
                                                              cgGGGAGTATGATGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGcc                                   <  2:784327/101‑1 (MQ=255)
                                                              cgGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGcc                                   >  2:4665242/1‑101 (MQ=255)
                                                              cgGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGcc                                   >  1:2313077/1‑101 (MQ=255)
                                                               ggggAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCcg                                  >  1:3621278/1‑101 (MQ=255)
                                                                   aGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGAc                              <  2:1301540/101‑1 (MQ=255)
                                                                   aGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGAc                              >  1:2110338/1‑101 (MQ=255)
                                                                     tATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCGTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAg                            >  2:2801484/1‑101 (MQ=255)
                                                                     tATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAg                            >  1:1598185/1‑101 (MQ=255)
                                                                     tATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAg                            >  2:1703050/1‑101 (MQ=255)
                                                                       tGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCg                          <  1:1018676/101‑1 (MQ=255)
                                                                         aGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTc                        <  1:3739241/101‑1 (MQ=255)
                                                                         aGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTc                        <  2:1370120/101‑1 (MQ=255)
                                                                         aGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCAACGGCCGCGACAGCGTc                        >  1:1086721/1‑101 (MQ=255)
                                                                          ggACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGGGTCg                       >  1:149661/1‑101 (MQ=255)
                                                                            aCAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGcc                     >  2:3051974/1‑101 (MQ=255)
                                                                            aCAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGcc                     <  1:2623126/101‑1 (MQ=255)
                                                                              aaGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGa                   >  1:4210833/1‑101 (MQ=255)
                                                                               aGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGAc                  <  2:1598080/101‑1 (MQ=255)
                                                                                    gggccgggcTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACACCGTCCCCGActccct             >  2:3680872/1‑101 (MQ=255)
                                                                                       ccgggcTGCGAGGACTGGCTGAGGGGTGTCGTCACGACCCCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTgcg          <  1:437168/101‑1 (MQ=255)
                                                                                        cgggcTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTgcgc         >  1:821190/1‑101 (MQ=255)
                                                                                        cgggcTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTgcgc         >  1:496365/1‑101 (MQ=255)
                                                                                         gggcTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGcgcg        >  1:2206061/1‑101 (MQ=255)
                                                                                         gggcTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGcgcg        >  2:4099771/1‑101 (MQ=255)
                                                                                          ggcTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCgg       >  2:1507704/1‑101 (MQ=255)
                                                                                          ggcTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCgg       >  2:829244/1‑101 (MQ=255)
                                                                                            cTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCGGCt     >  2:2932143/1‑101 (MQ=255)
                                                                                            cTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCGGCt     >  1:3648950/1‑101 (MQ=255)
                                                                                             tGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCGGCtt    >  2:3317982/1‑101 (MQ=255)
                                                                                              gCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCGGCTTc   >  1:2604665/1‑101 (MQ=255)
                                                                                              gCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCGGCTTc   <  2:717217/101‑1 (MQ=255)
                                                                                              gCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCCACTGCCTGCGCGGCTTc   >  2:4015010/1‑101 (MQ=255)
                                                                                               cGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCGGCTTCg  >  1:777061/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GCGCGTGGCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAAGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCGGCTTCG  >  NC_014034/243029‑243224

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG