Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,467,061 G→C 100% V29L (GTC→CTC)  RCAP_rcc02292 → signal transduction histidine kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,467,0610G→C100.0% 656.5 177V29L (GTC→CTC) RCAP_rcc02292signal transduction histidine kinase
Reads supporting (aligned to +/- strand):  new base (83/93):  ref base (0/0):  total (83/94)

Only 100 of 181 total aligned reads displayed.

GGGCCGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGGTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAGGT  >  NC_014034/2466963‑2467158
                                                                                                  |                                                                                                 
gggCCGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTc                                                                                                 >  1:2477521/1‑101 (MQ=255)
  gCCGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAg                                                                                               <  1:1447443/101‑1 (MQ=255)
    cGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCt                                                                                             <  1:4527814/101‑1 (MQ=255)
      cAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTAt                                                                                           >  2:656420/1‑101 (MQ=255)
      cAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTAt                                                                                           >  2:3994904/1‑101 (MQ=255)
       aGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATg                                                                                          <  2:794016/101‑1 (MQ=255)
        ggcggcATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATgc                                                                                         <  2:629050/101‑1 (MQ=255)
        ggcggcATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATgc                                                                                         <  1:1561795/101‑1 (MQ=255)
        ggcggcATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATgc                                                                                         <  2:2935117/101‑1 (MQ=255)
          gggcATGGTCCGGTGGCGCCGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATgcgc                                                                                       <  1:649431/100‑1 (MQ=255)
          cggcATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATgcgc                                                                                       <  2:2397620/101‑1 (MQ=255)
           ggcATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCa                                                                                      <  1:3792564/101‑1 (MQ=255)
           ggcATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCa                                                                                      >  1:924892/1‑101 (MQ=255)
            ncNNNATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCCGACGCCGCTCGCCGTGCTCAGCTATGCGCAg                                                                                     <  1:4131623/100‑1 (MQ=255)
            gcATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAg                                                                                     >  1:4628638/1‑101 (MQ=255)
             cATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGa                                                                                    <  2:1241692/101‑1 (MQ=255)
              aTGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGAc                                                                                   <  1:2924898/101‑1 (MQ=255)
              aTGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGAc                                                                                   >  2:4552139/1‑101 (MQ=255)
               tGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGAcc                                                                                  <  1:2794751/101‑1 (MQ=255)
               tGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGAcc                                                                                  <  1:4483050/101‑1 (MQ=255)
                 aTCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCt                                                                                <  2:3636082/101‑1 (MQ=255)
                  tCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTg                                                                               <  1:2369634/101‑1 (MQ=255)
                   ccGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTgg                                                                              <  1:1135027/101‑1 (MQ=255)
                    cGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGc                                                                             <  2:903815/101‑1 (MQ=255)
                     ggTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGcc                                                                            >  1:269261/1‑101 (MQ=255)
                     ggTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGcc                                                                            >  1:2749390/1‑101 (MQ=255)
                       tGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCAc                                                                          <  2:3496161/101‑1 (MQ=255)
                        ggCGCCGCGGCACGGGCGGTCGGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCAcc                                                                         <  2:4616721/101‑1 (MQ=255)
                        ggCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCAcc                                                                         <  1:2515261/101‑1 (MQ=255)
                        ggCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCAcc                                                                         <  2:161830/101‑1 (MQ=255)
                         gCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCg                                                                        <  2:2505845/101‑1 (MQ=255)
                          cGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGa                                                                       <  1:795149/101‑1 (MQ=255)
                          cGACGCGGCACGGGCGGTCTCGGGCTGCGGCTGACGGTGATCATGGCGGCGGTGCTGACGCCGCTCGCCGTGCTCATGTATGCGCAGACCCGGGCGACCGa                                                                       >  2:2650915/1‑101 (MQ=255)
                           gACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGaa                                                                      >  2:2670926/1‑101 (MQ=255)
                            aCGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCTGCTATGCGCAGACCCTGGCCACCGAAc                                                                     >  1:208403/1‑101 (MQ=255)
                            aCGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAAc                                                                     <  2:2366288/101‑1 (MQ=255)
                            aCGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAAc                                                                     <  1:1207722/101‑1 (MQ=255)
                             cgcgGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCGTGGCCACCGAACg                                                                    >  1:3128419/1‑101 (MQ=255)
                              gcgGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACgg                                                                   >  2:4326245/1‑101 (MQ=255)
                                ggCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGgc                                                                 <  1:3926094/101‑1 (MQ=255)
                                ggCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGgc                                                                 >  1:4060374/1‑101 (MQ=255)
                                     gggCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGccgccg                                                            >  2:4061247/1‑101 (MQ=255)
                                     gggCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGccgccg                                                            >  1:1045807/1‑101 (MQ=255)
                                     gggCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGccgccg                                                            >  1:4463233/1‑101 (MQ=255)
                                      ggCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGa                                                           >  1:2592485/1‑101 (MQ=255)
                                       gCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGaa                                                          <  1:2463526/101‑1 (MQ=255)
                                         ggTCTGGGGCTGCGGCTGGCGGGGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGaaaa                                                        <  2:771451/101‑1 (MQ=255)
                                           tCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAAcc                                                      <  2:3314699/101‑1 (MQ=255)
                                            cTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCg                                                     <  2:172829/101‑1 (MQ=255)
                                            cTGGGGCTGCGGCTGACGGGGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCCCCGAACGGGCCGCCGAAAACCg                                                     >  1:224929/1‑101 (MQ=255)
                                              ggggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTc                                                   >  2:4243432/1‑101 (MQ=255)
                                              ggggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTc                                                   >  2:1140195/1‑101 (MQ=255)
                                               gggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCt                                                  <  1:942651/101‑1 (MQ=255)
                                               gggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCt                                                  <  2:1959298/101‑1 (MQ=255)
                                                ggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTg                                                 <  1:3166013/101‑1 (MQ=255)
                                                ggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTg                                                 <  2:286836/101‑1 (MQ=255)
                                                    gCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTgcgcg                                             >  2:219660/1‑101 (MQ=255)
                                                    gCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTgcgcg                                             <  2:3291482/101‑1 (MQ=255)
                                                    gCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTgcgcg                                             >  2:4201737/1‑101 (MQ=255)
                                                      ggCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGcgcgcg                                           >  2:4098477/1‑101 (MQ=255)
                                                      ggCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGcgcgcg                                           <  1:1140583/101‑1 (MQ=255)
                                                       gCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCgcgg                                          <  1:3009539/101‑1 (MQ=255)
                                                        cTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCgcggc                                         >  2:315199/1‑101 (MQ=255)
                                                        cTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCgcggc                                         >  1:3270492/1‑101 (MQ=255)
                                                         tGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCgcggcg                                        >  2:2226160/1‑101 (MQ=255)
                                                          gACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGa                                       >  1:1871487/1‑101 (MQ=255)
                                                           aCGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCCGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGac                                      >  1:227223/1‑100 (MQ=255)
                                                           aCGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGAt                                      <  2:2793151/101‑1 (MQ=255)
                                                           aCGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGAt                                      <  2:4476630/101‑1 (MQ=255)
                                                           aCGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGAt                                      >  1:679988/1‑101 (MQ=255)
                                                             ggTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATcc                                    >  1:1642900/1‑101 (MQ=255)
                                                              gTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCt                                   >  1:1439322/1‑101 (MQ=255)
                                                                 aTCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTggg                                >  2:2975710/1‑101 (MQ=255)
                                                                  tCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGc                               <  1:1998125/101‑1 (MQ=255)
                                                                    aTGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGTga                             >  2:1347066/1‑101 (MQ=255)
                                                                      gggggCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCgaga                           >  1:1854270/1‑101 (MQ=255)
                                                                       ggggCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGAc                          <  2:3380224/101‑1 (MQ=255)
                                                                           cGGTGCTGACTCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTg                      <  2:3900868/101‑1 (MQ=255)
                                                                            ggTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGa                     <  1:1921592/101‑1 (MQ=255)
                                                                            ggTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAAACGTCTGCGCGCGGCGATCCTGGGGGGGACGCTGa                     >  2:828754/1‑101 (MQ=255)
                                                                             gTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGAt                    >  1:1685/1‑101 (MQ=255)
                                                                              tGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATg                   <  1:3684975/101‑1 (MQ=255)
                                                                                 tGACGCCGCTCGCCGTGCTCAGCTATGCGCAGCCCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgcc                <  2:3526009/101‑1 (MQ=255)
                                                                                 tGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgcc                <  2:1138991/101‑1 (MQ=255)
                                                                                 tGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgcc                >  2:3749875/1‑101 (MQ=255)
                                                                                 tGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgcc                >  2:2623091/1‑101 (MQ=255)
                                                                                 tGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgcc                <  1:4326537/101‑1 (MQ=255)
                                                                                    cgccgcTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgccgcc             >  2:1416528/1‑101 (MQ=255)
                                                                                     gccgcTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGccgccg            >  1:2434635/1‑101 (MQ=255)
                                                                                      ccgcTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCcgccgc           >  1:2142085/1‑101 (MQ=255)
                                                                                        gcTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCcgccgcgc         <  2:1649966/101‑1 (MQ=255)
                                                                                         cccGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCcgccgcgcc        <  2:1909712/99‑1 (MQ=255)
                                                                                         cTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCTCCGCACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCcgccgcgcc        <  1:3992385/101‑1 (MQ=255)
                                                                                         cTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCcgccgggcc        >  1:3324110/1‑101 (MQ=255)
                                                                                           cGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCgccgcgccgc      >  2:174097/1‑101 (MQ=255)
                                                                                             ccGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAg    <  1:1416623/101‑1 (MQ=255)
                                                                                              cGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAgg   >  2:1078216/1‑101 (MQ=255)
                                                                                              cGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAgg   >  2:3959947/1‑101 (MQ=255)
                                                                                              cGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAgg   <  1:2805129/101‑1 (MQ=255)
                                                                                               gTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAGGt  >  1:3095462/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GGGCCGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGGTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAGGT  >  NC_014034/2466963‑2467158

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG