Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,493,385 T→A 100% S53T (TCC→ACC)  yqgF → holliday junction resolvase YqgF

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,493,3850T→A100.0% 807.0 223S53T (TCC→ACC) yqgFholliday junction resolvase YqgF
Reads supporting (aligned to +/- strand):  new base (117/104):  ref base (0/0):  total (117/106)

Only 100 of 226 total aligned reads displayed.

CGGGCTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCTCCCTCGACCTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCGAGGGGCC  >  NC_014034/2493288‑2493482
                                                                                                 |                                                                                                 
 gggCTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCt                                                                                               <  1:2782148/101‑1 (MQ=255)
 gggCTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCt                                                                                               <  1:494269/101‑1 (MQ=255)
 gggCTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCt                                                                                               <  2:2434553/101‑1 (MQ=255)
 gggCTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCt                                                                                               >  1:1060535/1‑101 (MQ=255)
  ggCTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTc                                                                                              >  1:589438/1‑101 (MQ=255)
  ggCTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTc                                                                                              >  2:1631532/1‑101 (MQ=255)
   gCTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCg                                                                                             <  2:1084259/101‑1 (MQ=255)
    cTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGa                                                                                            >  1:2212323/1‑101 (MQ=255)
     tCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGAc                                                                                           <  2:1009844/101‑1 (MQ=255)
     tCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGAc                                                                                           <  1:734340/101‑1 (MQ=255)
      cGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGAcg                                                                                          <  1:31508/101‑2 (MQ=255)
        aTCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTg                                                                                        >  1:1703594/1‑101 (MQ=255)
         tCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTgg                                                                                       <  1:2455602/101‑1 (MQ=255)
         tCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTgg                                                                                       >  1:3020224/1‑101 (MQ=255)
            gggCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCgg                                                                                    >  1:1657415/1‑101 (MQ=255)
            gggCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCgg                                                                                    >  2:1098036/1‑101 (MQ=255)
                aCGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCt                                                                                <  1:1484821/101‑1 (MQ=255)
                aCGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCt                                                                                >  1:644420/1‑101 (MQ=255)
                 cGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCtg                                                                               <  1:2072496/101‑1 (MQ=255)
                  gAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCtga                                                                              >  1:2107517/1‑101 (MQ=255)
                  gAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCtga                                                                              >  2:1993402/1‑101 (MQ=255)
                   aaGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCtgat                                                                             <  2:4042522/101‑1 (MQ=255)
                      aCGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGaa                                                                          <  1:4559536/101‑1 (MQ=255)
                        gATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAgc                                                                        >  2:4305615/1‑100 (MQ=255)
                        gATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGa                                                                        <  2:731457/101‑1 (MQ=255)
                        gATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGa                                                                        <  1:4417182/101‑1 (MQ=255)
                          tCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGAcc                                                                      <  1:3376978/101‑1 (MQ=255)
                          tCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGAcc                                                                      <  1:340481/101‑1 (MQ=255)
                            ggggTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCgg                                                                    >  1:605251/1‑100 (MQ=255)
                            ggggTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGt                                                                    >  1:2609357/1‑101 (MQ=255)
                            ggggTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGt                                                                    <  1:3819491/101‑1 (MQ=255)
                             gggTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTg                                                                   <  1:3595294/101‑1 (MQ=255)
                             gggTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTg                                                                   <  1:677979/101‑1 (MQ=255)
                              ggTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTgg                                                                  <  1:1570016/101‑1 (MQ=255)
                              ggTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTgg                                                                  <  2:3837895/101‑1 (MQ=255)
                              ggTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTgg                                                                  <  2:2152202/101‑1 (MQ=255)
                               gTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGc                                                                 >  1:1310747/1‑101 (MQ=255)
                                tCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGcc                                                                <  1:1511172/101‑1 (MQ=255)
                                   cgGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAg                                                             <  1:4176753/101‑1 (MQ=255)
                                   cgGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAg                                                             <  2:1784949/101‑1 (MQ=255)
                                    ggTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGc                                                            <  1:354359/101‑1 (MQ=255)
                                     gTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCg                                                           <  1:1460706/101‑1 (MQ=255)
                                     gTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCg                                                           >  2:203651/1‑101 (MQ=255)
                                        tcGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCa                                                        >  2:2758959/1‑101 (MQ=255)
                                         cGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCaa                                                       >  2:2372384/1‑101 (MQ=255)
                                         cGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCaa                                                       >  2:1749503/1‑101 (MQ=255)
                                           gACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAAt                                                     >  2:2532870/1‑101 (MQ=255)
                                               cGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGcc                                                 <  1:2759157/101‑1 (MQ=255)
                                                gCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCg                                                >  1:857104/1‑101 (MQ=255)
                                                    cGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGtt                                            >  1:1270111/1‑101 (MQ=255)
                                                     ggggCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTc                                           <  2:3171651/101‑1 (MQ=255)
                                                      gggCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCg                                          >  1:1728795/1‑101 (MQ=255)
                                                         cATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTcc                                       >  2:1576118/1‑101 (MQ=255)
                                                         cATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTcc                                       >  1:3438112/1‑101 (MQ=255)
                                                          aTCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCt                                      >  2:4259550/1‑101 (MQ=255)
                                                          aTCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCt                                      >  1:2008020/1‑101 (MQ=255)
                                                          aTCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCt                                      >  2:2385308/1‑101 (MQ=255)
                                                          aTCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCt                                      <  2:3502048/101‑1 (MQ=255)
                                                            cgcgACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCg                                    <  1:1022808/101‑1 (MQ=255)
                                                            cgcgACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCg                                    >  2:1878690/1‑101 (MQ=255)
                                                             gcgACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCgg                                   >  2:2874015/1‑101 (MQ=255)
                                                              cgACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCggg                                  >  1:2988915/1‑101 (MQ=255)
                                                              cgACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCggg                                  >  1:399101/1‑101 (MQ=255)
                                                               gaagcaccTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGAGGATCTGATGAAGACCGTGGCCGAGCGGCAACTCGCCGTGCTCCTCCTCGGGc                                 >  2:715845/7‑101 (MQ=255)
                                                               gACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGc                                 >  1:4550654/1‑101 (MQ=255)
                                                                gcGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCt                                <  2:4420019/100‑1 (MQ=255)
                                                                aCGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCt                                >  1:1122660/1‑101 (MQ=255)
                                                                  gCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGc                              >  1:1001163/1‑101 (MQ=255)
                                                                   ccccTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGcc                             >  2:3570610/1‑101 (MQ=255)
                                                                    cccTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCg                            >  1:3833849/1‑101 (MQ=255)
                                                                      cTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGt                          >  2:1502522/1‑101 (MQ=255)
                                                                         cgcACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGaa                       <  1:3647751/101‑1 (MQ=255)
                                                                         cgcACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGaa                       >  1:2883792/1‑101 (MQ=255)
                                                                          gcACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAAc                      >  2:3915529/1‑101 (MQ=255)
                                                                          gcACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAAc                      >  2:4659206/1‑101 (MQ=255)
                                                                          gcACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAAc                      >  2:4092648/1‑101 (MQ=255)
                                                                           cACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGGTGCCGGTGAACa                     >  2:4460839/1‑101 (MQ=255)
                                                                           cACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACa                     >  2:1341081/1‑101 (MQ=255)
                                                                           cACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACa                     >  1:1082368/1‑101 (MQ=255)
                                                                           cACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACa                     >  2:3276681/1‑101 (MQ=255)
                                                                             cGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATg                   >  2:2992487/1‑101 (MQ=255)
                                                                              gATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATgg                  >  1:2201792/1‑101 (MQ=255)
                                                                              gATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATgg                  >  2:1161236/1‑101 (MQ=255)
                                                                               aTCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGa                 <  2:250509/101‑1 (MQ=255)
                                                                               aTCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGa                 <  2:812945/101‑1 (MQ=255)
                                                                               aTCCGGAGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGCGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATggc                 >  2:3551875/1‑100 (MQ=255)
                                                                                tCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATgggc                >  2:657512/1‑99 (MQ=255)
                                                                                tCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGAc                >  1:3688198/1‑101 (MQ=255)
                                                                                 ccGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACg               <  2:1060446/101‑1 (MQ=255)
                                                                                      gcgAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTcc          <  2:3036025/101‑1 (MQ=255)
                                                                                      gcgAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTcc          <  1:4314414/101‑1 (MQ=255)
                                                                                      gcgAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGGGAACATGGACGGCTcc          >  2:1762713/1‑101 (MQ=255)
                                                                                       cgAGAAATTCCCCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCg         <  2:3048633/101‑1 (MQ=255)
                                                                                       cgAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCg         >  1:361236/1‑101 (MQ=255)
                                                                                       cgAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCg         <  1:3743808/101‑1 (MQ=255)
                                                                                       cgAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCg         <  2:4369390/101‑1 (MQ=255)
                                                                                       cgAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCg         >  1:1376854/1‑101 (MQ=255)
                                                                                            aaTTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCGAgggg    <  1:4376115/101‑1 (MQ=255)
                                                                                              ttCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCGAGGGGcc  <  2:3437858/101‑1 (MQ=255)
                                                                                              ttCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCGAGGGGcc  <  2:4377075/101‑1 (MQ=255)
                                                                                                 |                                                                                                 
CGGGCTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCTCCCTCGACCTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCGAGGGGCC  >  NC_014034/2493288‑2493482

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG