Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,015 C→G 6.4% intergenic (+63/‑179) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0150C→G 6.4% 15.7 ‑1.7 ‑0.5140intergenic (+63/‑179)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/3):  ref base (55/76):  total (61/79)
Fisher's exact test strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.01e-01

Only 100 of 151 total aligned reads displayed.

CGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGGCGCT.GCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCT  >  NC_014034/2493917‑2494111
                                                                                                    |                                                                                                       
ctccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                                                                                        <  2:720224/99‑1 (MQ=18)
cgccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGGTATTTGCAGCAAGATGAAAcc                                                                                                        >  2:3893179/1‑101 (MQ=21)
 gccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGGGGCTCGCCCCCG.TCCGCAAGCGGGCTCCCCCCGCGATATGTTCAGGAAGGTGAAAcac                                                                                                       >  2:3752442/1‑99 (MQ=16)
   cgcgGCGGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                                                                     <  1:3827145/101‑1 (MQ=21)
   cgcgGCGGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                                                                     <  2:3085716/101‑1 (MQ=21)
   cgcgGCGGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                                                                     <  1:2415967/101‑1 (MQ=21)
   cgcgCCGGGCGCGCGCCCCGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                                                                     <  2:4138218/101‑1 (MQ=17)
    gcggcgGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAt                                                                                                    <  1:3873785/101‑1 (MQ=21)
    gcggcgGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAt                                                                                                    <  2:3799038/101‑1 (MQ=21)
     cggcggGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATg                                                                                                   <  1:2093370/101‑1 (MQ=21)
      ggcgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCACCGGGAGATTTGCAGCAAGATGAAACAGGCTGc                                                                                                  >  1:4013220/1‑101 (MQ=18)
       gcgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGcc                                                                                                 <  2:3405661/101‑1 (MQ=21)
        cgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCt                                                                                                <  1:576105/101‑1 (MQ=21)
         gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                               <  2:3907789/101‑1 (MQ=21)
          ggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCGCGTCCGCAAGCGGACTCCCCCCGGGGTATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                              <  1:1624367/101‑1 (MQ=21)
          ggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                              <  2:1947490/101‑1 (MQ=21)
           gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                             <  2:363510/101‑1 (MQ=21)
           gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                             <  1:4452450/101‑1 (MQ=21)
              cgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                          >  2:2974540/1‑101 (MQ=21)
               gcgGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                         <  1:2334011/101‑1 (MQ=21)
                cgGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                        <  2:1020681/101‑1 (MQ=21)
                cgGCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                        >  2:1188154/1‑101 (MQ=21)
                 ggCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                       >  2:1364262/1‑101 (MQ=21)
                 ggCACGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                       >  2:2736730/1‑101 (MQ=21)
                     cGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                   <  1:1254986/101‑1 (MQ=21)
                     cGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                   <  2:4362146/101‑1 (MQ=21)
                     cGACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                   >  2:4046907/1‑101 (MQ=21)
                      gACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGa                                                                                  <  2:2833658/101‑1 (MQ=21)
                      gACAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGa                                                                                  <  1:3525447/101‑1 (MQ=21)
                       aCAGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaa                                                                                 <  2:4427230/101‑1 (MQ=21)
                         aGGCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                               <  2:647773/101‑1 (MQ=21)
                          ggCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                              <  2:3559894/101‑1 (MQ=21)
                           gCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                             <  2:1324367/101‑1 (MQ=21)
                           gCCGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                             <  1:3778516/101‑1 (MQ=21)
                            tcGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                            <  1:4054669/100‑1 (MQ=18)
                            ccGAGTAGGGGGGGGGCGCT.CCCCCCCGTCCGCAAGCGGACCCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                            <  2:2063019/101‑1 (MQ=18)
                             cGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                           >  2:31263/1‑101 (MQ=18)
                             cGAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                           <  1:1686562/101‑1 (MQ=18)
                              gAGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                          >  1:3418496/1‑101 (MQ=18)
                               aGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                         <  1:1833890/101‑1 (MQ=18)
                               aGTAAGGGGGGGGCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                         >  1:1000152/1‑101 (MQ=18)
                                gtaaggggggggCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGGTATTTGCAGCAAGATGAAAACGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                        >  1:906249/6‑101 (MQ=17)
                                gtaaggggggggCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                        <  1:1350477/96‑1 (MQ=255)
                                gtaaggggggggCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                        >  1:1486152/6‑101 (MQ=255)
                                  aaggggggggGCCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                      <  2:1000069/98‑1 (MQ=18)
                                  aaggggggggCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                      >  1:1628290/4‑101 (MQ=255)
                                    ggggggggCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                    >  1:2629620/2‑101 (MQ=255)
                                     gggggggCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                   <  2:960816/101‑1 (MQ=255)
                                      ggggggCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggggg                                                                  >  2:1621830/1‑99 (MQ=255)
                                      ggggggCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                  <  1:1668650/101‑1 (MQ=255)
                                      ggggggCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                  <  1:2172571/101‑1 (MQ=255)
                                      ggggggCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                  <  1:4511415/101‑1 (MQ=255)
                                       gggggCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                 >  1:3823557/1‑101 (MQ=255)
                                         gggCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                              <  1:4515500/101‑1 (MQ=255)
                                          ggCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                             <  1:4340028/101‑1 (MQ=255)
                                          ggCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCAGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                             <  1:3232344/101‑1 (MQ=255)
                                             gcT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                        >  2:2159991/1‑101 (MQ=255)
                                              cT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGG..G.a                                                      >  1:4349045/1‑101 (MQ=18)
                                               t.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                     >  1:14024/1‑100 (MQ=255)
                                                 gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                    <  1:704842/101‑3 (MQ=255)
                                                 gCCCCCCGTCCGCAAGCGGAATCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.GGGC..G.aac                                                    >  2:2183415/1‑99 (MQ=18)
                                                  cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                  >  1:422015/1‑97 (MQ=255)
                                                  cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                  <  1:589397/101‑5 (MQ=255)
                                                  cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                   <  1:198496/101‑4 (MQ=255)
                                                   ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                 <  2:3670665/101‑6 (MQ=255)
                                                     cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                <  1:509831/97‑7 (MQ=255)
                                                     cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccag                                                >  1:2985216/5‑95 (MQ=18)
                                                      ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                               <  2:432097/98‑8 (MQ=255)
                                                       cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                              <  1:2418397/99‑9 (MQ=255)
                                                        gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                             >  2:4129599/2‑92 (MQ=255)
                                                               aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCag                                     <  2:1264520/101‑2 (MQ=18)
                                                                 cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCagcg                                   >  1:2943247/1‑98 (MQ=18)
                                                                  ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCagcgc                                  >  1:798133/1‑97 (MQ=17)
                                                                    aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGG..GAAG.CCAG.AGGGGGGC.AGCG.ccc                              >  2:4255159/1‑101 (MQ=17)
                                                                    aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc                              >  2:2256315/1‑101 (MQ=18)
                                                                     cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cccc                             >  2:486759/1‑101 (MQ=18)
                                                                      tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG..AGGGGGGC.AGCG.ccccc                            <  2:1714876/101‑1 (MQ=17)
                                                                        cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cccccct                          <  1:2160141/101‑3 (MQ=17)
                                                                        cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cccccct                          <  2:2243612/101‑3 (MQ=17)
                                                                         ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cccccctg                         <  2:2984987/101‑4 (MQ=17)
                                                                         ccccGGGATATCTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cccccctg                         >  1:2729047/1‑98 (MQ=17)
                                                                            cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AA.GACAGAGGGGGGC.AGCG.cccccctgacc                      >  1:1814513/1‑95 (MQ=17)
                                                                             gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCAGCAC.CCCCCTGcccc                      >  2:344576/1‑101 (MQ=17)
                                                                              ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcg                     >  1:1282178/1‑101 (MQ=2)
                                                                              ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcg                     >  2:1903843/1‑101 (MQ=0)
                                                                               gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgc                    >  2:4290240/1‑100 (MQ=2)
                                                                                atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgcc                   <  2:2444885/101‑3 (MQ=2)
                                                                                atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgcc                   <  1:1127028/101‑3 (MQ=2)
                                                                                 tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGACCcggcg                  >  2:1611164/1‑101 (MQ=17)
                                                                                    ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCAGCGG.CCCCCGGCCCCGGCGGTc               >  2:714330/1‑101 (MQ=18)
                                                                                    ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTc               <  1:1467624/101‑1 (MQ=17)
                                                                                     tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc              <  2:1703597/101‑1 (MQ=17)
                                                                                     tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc              >  1:3926664/1‑101 (MQ=18)
                                                                                      gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAC.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg             >  1:4037011/1‑101 (MQ=18)
                                                                                       cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.CCCCCCGCCCCCGCGGGcccgg           >  1:4092509/1‑98 (MQ=17)
                                                                                       cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAG.CCAC.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGt            >  1:2256617/1‑101 (MQ=18)
                                                                                         gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTgg          <  2:2629435/101‑1 (MQ=17)
                                                                                          caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggc         >  2:2049065/1‑101 (MQ=18)
                                                                                               tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGgcgc    >  1:4604971/1‑101 (MQ=17)
                                                                                                 aaaCCGGATGCCTTTCATCTTCCCCGAAATGTCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCt  <  2:3182635/101‑1 (MQ=17)
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CGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGGCGCT.GCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCT  >  NC_014034/2493917‑2494111

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG