Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,018 G→C 6.6% intergenic (+66/‑176) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0180G→C 6.6% 16.1 ‑1.8 ‑0.5139intergenic (+66/‑176)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/3):  ref base (53/76):  total (60/79)
Fisher's exact test strand distribution p-value = 1.71e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.90e-01

Only 100 of 147 total aligned reads displayed.

CGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGA.AGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGG  >  NC_014034/2493920‑2494117
                                                                                                   |                                                                                                         
cgcgGCGGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                                                                          <  1:3827145/101‑1 (MQ=21)
cgcgGCGGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                                                                          <  2:3085716/101‑1 (MQ=21)
cgcgCCGGGCGCGCGCCCCGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                                                                          <  2:4138218/101‑1 (MQ=17)
 gcggcgGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAt                                                                                                         <  1:3873785/101‑1 (MQ=21)
  cggcggGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATg                                                                                                        <  1:2093370/101‑1 (MQ=21)
   ggcgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCACCGGGAGATTTGCAGCAAGATGAAACAGGCTGc                                                                                                       >  1:4013220/1‑101 (MQ=18)
    gcgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGcc                                                                                                      <  2:3405661/101‑1 (MQ=21)
     cgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCt                                                                                                     <  1:576105/101‑1 (MQ=21)
      gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                    <  2:3691524/101‑1 (MQ=21)
      gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                    <  2:3907789/101‑1 (MQ=21)
       ggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCGCGTCCGCAAGCGGACTCCCCCCGGGGTATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                   <  1:1624367/101‑1 (MQ=21)
        gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                  <  1:4452450/101‑1 (MQ=21)
        gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                  <  2:363510/101‑1 (MQ=21)
           cgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                               >  2:2974540/1‑101 (MQ=21)
            gcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                              <  1:2334011/101‑1 (MQ=21)
             cgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                             <  1:2097046/101‑1 (MQ=21)
             cgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                             >  2:1188154/1‑101 (MQ=21)
              ggCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                            >  2:2736730/1‑101 (MQ=21)
                cACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc                                                                                          >  2:1311081/1‑101 (MQ=21)
                  cGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                        <  1:2796169/101‑1 (MQ=21)
                  cGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                        <  1:2581620/101‑1 (MQ=21)
                  cGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                        <  1:1254986/101‑1 (MQ=21)
                  cGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                        >  2:4046907/1‑101 (MQ=21)
                  cGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                        <  2:4362146/101‑1 (MQ=21)
                   gACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGa                                                                                       <  2:110926/101‑1 (MQ=21)
                   gACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGa                                                                                       <  2:2833658/101‑1 (MQ=21)
                   gACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGACGCCTTTCATCTTTGCCGa                                                                                       >  2:1674435/1‑101 (MQ=17)
                    aCAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaa                                                                                      <  2:4427230/101‑1 (MQ=21)
                     cAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                     <  2:1981220/101‑1 (MQ=21)
                      aGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                                    <  2:647773/101‑1 (MQ=21)
                      aGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                                    <  1:3816435/101‑1 (MQ=21)
                       ggCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                   <  2:3559894/101‑1 (MQ=21)
                        gCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                                  <  1:3778516/101‑1 (MQ=21)
                        gCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                                  <  2:1324367/101‑1 (MQ=21)
                         tcGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                                 <  1:4054669/100‑1 (MQ=18)
                          cGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                <  1:1686562/101‑1 (MQ=18)
                          cGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                >  2:31263/1‑101 (MQ=18)
                           gAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                               >  1:3418496/1‑101 (MQ=18)
                            aGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                              <  1:1833890/101‑1 (MQ=18)
                            aGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                              >  1:1000152/1‑101 (MQ=18)
                            aGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAAGCATTTAATCTTGCCAGAAATATTCCg                                                                              >  2:187457/1‑101 (MQ=17)
                             gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                             >  1:1486152/6‑101 (MQ=255)
                             gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                             <  1:1350477/96‑1 (MQ=255)
                               aaggggggggGCCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                           <  2:1000069/98‑1 (MQ=18)
                               aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                           >  1:1628290/4‑101 (MQ=255)
                                  gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                        <  2:960816/101‑1 (MQ=255)
                                   ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                       <  1:1668650/101‑1 (MQ=255)
                                   ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                       <  1:2172571/101‑1 (MQ=255)
                                   ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                       <  1:4511415/101‑1 (MQ=255)
                                      gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                   <  1:4515500/101‑1 (MQ=255)
                                       ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                  <  1:4340028/101‑1 (MQ=255)
                                       ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCAGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                  <  1:3232344/101‑1 (MQ=255)
                                          gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                             >  2:2159991/1‑101 (MQ=255)
                                           cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGG..G.a                                                           >  1:4349045/1‑101 (MQ=18)
                                            tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                          >  1:14024/1‑100 (MQ=255)
                                             gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                         <  1:704842/101‑3 (MQ=255)
                                             gCCCCCCGTCCGCAAGCGGAATCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.GGGC..G.aac                                                         >  2:2183415/1‑99 (MQ=18)
                                              cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                       <  1:589397/101‑5 (MQ=255)
                                              cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                       >  1:422015/1‑97 (MQ=255)
                                              cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                       <  2:1568902/101‑5 (MQ=255)
                                              cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                        <  1:198496/101‑4 (MQ=255)
                                               ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                      <  2:3670665/101‑6 (MQ=255)
                                                 cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                     <  1:509831/97‑7 (MQ=255)
                                                 cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccag                                                     >  1:2985216/5‑95 (MQ=18)
                                                  ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                    <  2:432097/98‑8 (MQ=255)
                                                   cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                   <  1:2418397/99‑9 (MQ=255)
                                                    gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                  >  2:4129599/2‑92 (MQ=255)
                                                          aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGCa                                            <  1:814009/101‑1 (MQ=18)
                                                            gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGGCTTTCATCTTGCCCGAAATGTCAACGGGGGGGAA.TGGC..GAAGCCAG.AGGGGGGCagc                                          >  1:3989609/1‑99 (MQ=17)
                                                              ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGCagcgc                                        >  1:798133/1‑97 (MQ=17)
                                                                aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGG..GAAGCCAG.AGGGGGGC.AGCG.ccc                                    >  2:4255159/1‑101 (MQ=17)
                                                                aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCG.ccc                                    >  2:2256315/1‑101 (MQ=18)
                                                                 cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCG.cccc                                   >  2:486759/1‑101 (MQ=18)
                                                                  tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCG.ccccc                                  >  1:2173295/1‑101 (MQ=18)
                                                                    cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCG.cccccct                                <  2:2243612/101‑3 (MQ=17)
                                                                     ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCG.cccccctg                               <  2:2984987/101‑4 (MQ=17)
                                                                     ccccGGGATATCTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCG.cccccctg                               >  1:2729047/1‑98 (MQ=17)
                                                                        cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AAGACAGAGGGGGGC.AGCG.cccccctgacc                            >  1:1814513/1‑95 (MQ=17)
                                                                         gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGCAGCAC.CCCCCTGcccc                            >  2:344576/1‑101 (MQ=17)
                                                                          ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCA.AGAGGGGGCAGCG.CCCCCTGCCCcg                           <  1:2338829/101‑1 (MQ=0)
                                                                          ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCA.AGAGGGGGCAGCG.CCCCCTGCCCcg                           >  2:1903843/1‑101 (MQ=0)
                                                                          ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCA.AGAGGGGGCAGCG.CCCCCTGCCCcg                           >  1:1282178/1‑101 (MQ=2)
                                                                          ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCCCCCCTGCCCcg                           >  1:991533/1‑101 (MQ=18)
                                                                           gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgc                          >  2:4290240/1‑100 (MQ=2)
                                                                            atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgcc                         <  1:1127028/101‑3 (MQ=2)
                                                                            atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgcc                         <  2:2444885/101‑3 (MQ=2)
                                                                            atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgcc                         >  1:3869648/1‑99 (MQ=0)
                                                                             tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgccg                        <  2:2091493/101‑1 (MQ=2)
                                                                             tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCCCCCCTGACCcggcg                        >  2:1611164/1‑101 (MQ=17)
                                                                                ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGCAGCGG.CCCCCGGCCCCGGCGGTc                     >  2:714330/1‑101 (MQ=18)
                                                                                ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTc                     <  1:1467624/101‑1 (MQ=17)
                                                                                 tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                    <  2:1703597/101‑1 (MQ=17)
                                                                                   cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCG.CCCCCCGCCCCCGCGGGcccgg                 >  1:4092509/1‑98 (MQ=17)
                                                                                     gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTgg                <  2:2629435/101‑1 (MQ=17)
                                                                                      caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggc               >  2:2049065/1‑101 (MQ=18)
                                                                                        aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg             <  2:3823272/101‑1 (MQ=17)
                                                                                           tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGgcgc          >  1:4604971/1‑101 (MQ=17)
                                                                                             aaaCCGGATGCCTTTCATCTTCCCCGAAATGTCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCt        <  2:3182635/101‑1 (MQ=17)
                                                                                                  ggATGCCTTTCATCTTTCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg   <  1:3085165/101‑1 (MQ=17)
                                                                                                   gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg  >  1:1199947/1‑101 (MQ=17)
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CGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGA.AGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGG  >  NC_014034/2493920‑2494117

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG