Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,048 G→A 8.8% intergenic (+96/‑146) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0480G→A 8.8% 18.9 ‑0.3 ‑0.4114intergenic (+96/‑146)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/4):  ref base (56/48):  total (62/52)
Fisher's exact test strand distribution p-value = 7.53e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.97e-01

Only 100 of 134 total aligned reads displayed.

AGTAAGGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCA  >  NC_014034/2493948‑2494147
                                                                                                    |                                                                                                           
gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                                             <  1:1350477/96‑1 (MQ=255)
gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                                             >  1:1486152/6‑101 (MQ=255)
 taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCCGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                                            >  1:3180596/5‑101 (MQ=18)
  aaggggggggGCCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                                                           <  2:1000069/98‑1 (MQ=18)
  aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                                                           >  1:1628290/4‑101 (MQ=255)
    ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                                                         >  1:2629620/2‑101 (MQ=255)
     gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggggg                                                                                                        >  2:3028533/1‑100 (MQ=255)
     gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                                                        <  2:960816/101‑1 (MQ=255)
      ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                                                       <  1:1668650/101‑1 (MQ=255)
      ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                                                       <  1:2172571/101‑1 (MQ=255)
       gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                                                      <  2:2208328/101‑1 (MQ=255)
       gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                                                      >  1:3823557/1‑101 (MQ=255)
         gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                   <  1:4515500/101‑1 (MQ=255)
          ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCAGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                                                  <  1:3232344/101‑1 (MQ=255)
              cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGG..G.a                                                                                           >  1:4349045/1‑101 (MQ=18)
               tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                                                          >  1:14024/1‑100 (MQ=255)
                gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGCTATTTGCAGCAAGATGCAACCGAATGCCTTTCATCTTGCCCGCAATATCCCGGGGGGAGAA.GGGG..G.aag                                                                                         >  1:124217/1‑99 (MQ=18)
                gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                         <  1:704842/101‑3 (MQ=255)
                gCCCCCCGTCCGCAAGCGGAATCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.GGGC..G.aac                                                                                         >  2:2183415/1‑99 (MQ=18)
                 cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                       <  2:1568902/101‑5 (MQ=255)
                 cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                       <  1:589397/101‑5 (MQ=255)
                 cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                       <  1:434851/101‑5 (MQ=255)
                 cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                                                        <  1:198496/101‑4 (MQ=255)
                  ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                                                      <  2:3670665/101‑6 (MQ=255)
                     ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                                                    <  2:432097/98‑8 (MQ=255)
                       gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                                                  >  2:4129599/2‑92 (MQ=255)
                             aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCa                                                                           <  1:814009/101‑1 (MQ=18)
                              aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCag                                                                          <  2:1264520/101‑2 (MQ=18)
                               gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGGCTTTCATCTTGCCCGAAATGTCAACGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGCagc                                                                         >  1:3989609/1‑99 (MQ=17)
                                cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCagcg                                                                        >  1:2943247/1‑98 (MQ=18)
                                   aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGG..GAAG.CCAG.AGGGGGGC.AGCG.ccc                                                                   >  2:4255159/1‑101 (MQ=17)
                                   aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc                                                                   >  2:2256315/1‑101 (MQ=18)
                                    cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc.c                                                                 >  2:486759/1‑101 (MQ=18)
                                     tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG..AGGGGGGC.AGCG.ccc.cc                                                                <  2:1714876/101‑1 (MQ=17)
                                       cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc.ccct                                                              <  1:2160141/101‑3 (MQ=17)
                                        ccccGGGATATCTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc.ccctg                                                             >  1:2729047/1‑98 (MQ=17)
                                           cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AA.GACAGAGGGGGGC.AGCG.ccc.ccctgacc                                                          >  1:1814513/1‑95 (MQ=17)
                                            gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCAGCAC.CCC.CCTGcccc                                                          >  2:344576/1‑101 (MQ=17)
                                             ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcg                                                         <  1:2338829/101‑1 (MQ=0)
                                             ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcg                                                         >  2:1903843/1‑101 (MQ=0)
                                             ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCcg                                                         >  2:4256564/1‑101 (MQ=17)
                                             ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCcg                                                         >  1:991533/1‑101 (MQ=18)
                                               atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcgcc                                                       <  2:2444885/101‑3 (MQ=2)
                                               atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcgcc                                                       <  1:1127028/101‑3 (MQ=2)
                                                tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcgccg                                                      <  2:2091493/101‑1 (MQ=2)
                                                tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGT..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCcggcg                                                      >  1:1457259/1‑101 (MQ=17)
                                                tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGACCcggcg                                                      >  2:1611164/1‑101 (MQ=17)
                                                 atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcgccgg                                                     >  1:4479250/1‑101 (MQ=2)
                                                   ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCAGCGG.CCC.CCGGCCCCGGCGGTc                                                   >  2:714330/1‑101 (MQ=18)
                                                   ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTc                                                   <  1:1467624/101‑1 (MQ=17)
                                                    tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTcc                                                  >  1:3926664/1‑101 (MQ=18)
                                                    tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTcc                                                  <  2:1703597/101‑1 (MQ=17)
                                                     gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCg                                                 >  1:1658069/1‑101 (MQ=17)
                                                     gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAC.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCg                                                 >  1:4037011/1‑101 (MQ=18)
                                                      cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGt                                                >  2:4340374/1‑101 (MQ=18)
                                                      cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.CCC.CCCGCCCCCGCGGGcccgg                                               >  1:4092509/1‑98 (MQ=17)
                                                      cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAG.CCAC.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGt                                                >  1:2256617/1‑101 (MQ=18)
                                                        gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAG.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTgg                                              >  2:294835/1‑101 (MQ=18)
                                                        gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTgg                                              <  2:2629435/101‑1 (MQ=17)
                                                         caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTggc                                             >  2:2049065/1‑101 (MQ=18)
                                                         caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.TGGC..GAAG.CCAG.AGGGGGGCCGACC.CCC.CCTGCCCCGGCGGTCCGCggg                                             >  1:677081/1‑100 (MQ=17)
                                                           aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTggcgg                                           <  2:3823272/101‑1 (MQ=17)
                                                           aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGG.AAGCGCCCC.CCTGCCCCGGCGGTCCGGggcgg                                           >  1:1331463/1‑101 (MQ=17)
                                                              tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGgcgc                                        >  1:4604971/1‑101 (MQ=17)
                                                                aaaCCGGATGCCTTTCATCTTCCCCGAAATGTCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCt                                      <  2:3182635/101‑1 (MQ=17)
                                                                  ccgggctGCCTTTAATCTTG.CCAAAACTTGCCGGGGGGGGAAGGGGC..GAAG.CGGG.GGGGGGCCACCCC.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                    <  2:2728849/95‑1 (MQ=255)
                                                                     ggATGCCTTTCATCTTTCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                 <  1:3085165/101‑1 (MQ=17)
                                                                      gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                >  1:1199947/1‑101 (MQ=17)
                                                                        tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                              >  2:3668466/1‑101 (MQ=18)
                                                                         gCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcg                             >  1:3532805/1‑101 (MQ=18)
                                                                           cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                           <  2:3699520/101‑1 (MQ=17)
                                                                           cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                           <  2:2173145/101‑1 (MQ=17)
                                                                           cTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                           >  1:4153737/1‑101 (MQ=18)
                                                                            tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                          <  2:3939830/101‑1 (MQ=17)
                                                                             ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                         >  2:2317350/1‑101 (MQ=18)
                                                                               cATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGG..GAAG.TCAA.GGGGGGGCAGGCC.CCC.CCTAGCCAGGGCGTCCGGGGCGGGCCCTGGAGGGGGggggggg                       >  1:2574823/1‑99 (MQ=9)
                                                                                aTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcgc                      <  1:31269/101‑1 (MQ=18)
                                                                                 tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                     >  1:3698092/1‑101 (MQ=18)
                                                                                  cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCgg                    >  1:2097304/1‑101 (MQ=18)
                                                                                  cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                    >  2:2565296/1‑101 (MQ=18)
                                                                                  cTTGCCCGAAATATCCCGGGGGGAGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGGGCCTTGCCGCGCTGGGGCGCgg                    >  1:3664851/1‑101 (MQ=12)
                                                                                    tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGc                  <  2:2943018/101‑1 (MQ=18)
                                                                                       ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt               <  1:2906827/101‑1 (MQ=18)
                                                                                         gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCagatc             >  2:2489016/1‑97 (MQ=18)
                                                                                         gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa             <  1:2256477/101‑1 (MQ=18)
                                                                                         gAAATATCCCGGGGGGTGAA.TCGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa             <  1:4255447/101‑1 (MQ=18)
                                                                                         gAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGCATCGC.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGTGGGCGTGGGCATTaa             >  2:449851/1‑101 (MQ=21)
                                                                                         gAAATATCCCGGGGGGGGAA.AGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa             >  2:2097177/1‑101 (MQ=18)
                                                                                          aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc            >  1:1967380/1‑101 (MQ=18)
                                                                                          aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc            <  1:4330329/101‑1 (MQ=18)
                                                                                           aaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc           >  2:584359/1‑101 (MQ=21)
                                                                                              atCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGGATTAACCGaa        >  1:2619658/1‑101 (MQ=21)
                                                                                               tCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa       <  1:867912/101‑1 (MQ=21)
                                                                                               tCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa       <  2:14019/101‑1 (MQ=21)
                                                                                                cccGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAt      >  1:890800/1‑101 (MQ=21)
                                                                                                cccGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAt      <  2:4246277/101‑1 (MQ=21)
                                                                                                  cGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTg    <  1:3893464/101‑1 (MQ=25)
                                                                                                  cGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTg    >  2:815951/1‑101 (MQ=25)
                                                                                                   ggggggTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.CCCACCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc   <  1:3245406/101‑1 (MQ=25)
                                                                                                    gggggTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa  >  1:4286504/1‑101 (MQ=25)
                                                                                                    |                                                                                                           
AGTAAGGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCA  >  NC_014034/2493948‑2494147

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG