Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,523,952 T→G 100% I573S (ATC→AGC)  ppiD → peptidyl‑prolyl cis‑trans isomerase D

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,523,9520T→G100.0% 836.8 220I573S (ATC→AGC) ppiDpeptidyl‑prolyl cis‑trans isomerase D
Reads supporting (aligned to +/- strand):  new base (117/103):  ref base (0/0):  total (117/103)

Only 100 of 228 total aligned reads displayed.

CGACAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGATCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCAA  >  NC_014034/2523854‑2524049
                                                                                                  |                                                                                                 
cGACAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGcc                                                                                                 >  1:2065658/1‑101 (MQ=255)
cGACAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGcc                                                                                                 >  2:2728047/1‑101 (MQ=255)
   cAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGa                                                                                              <  1:1653993/101‑1 (MQ=255)
   cAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGa                                                                                              >  1:335189/1‑101 (MQ=255)
   cAAGGTCTTTGTCGTCACCGTGCGCGCGGGGAAACCGGCCGATCCGGCCGATCACGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGa                                                                                              >  1:3032658/1‑101 (MQ=255)
    ttccgatctGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGAt                                                                                             <  1:2492334/93‑1 (MQ=255)
    aaGGTCTTTGTCGTCACCGTGCGCGGGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGAt                                                                                             <  1:1580764/101‑1 (MQ=255)
     aGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATg                                                                                            >  2:750595/1‑101 (MQ=255)
     aGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATg                                                                                            <  2:4541549/101‑1 (MQ=255)
     aGGTCTTTGTCGTCACCGTGCGCGCGGGGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATg                                                                                            <  2:2310542/101‑1 (MQ=255)
        tCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTc                                                                                         >  2:1708555/1‑101 (MQ=255)
         cTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCg                                                                                        <  1:3319356/101‑1 (MQ=255)
         cTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCg                                                                                        <  2:477356/101‑1 (MQ=255)
          tttGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCgg                                                                                       >  1:1358846/1‑101 (MQ=255)
          tttGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCgg                                                                                       >  2:4298730/1‑101 (MQ=255)
           ttGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCCAGACCTTTGACACCCGGCTGAGCCAGATGCTCGgc                                                                                      >  1:4441815/1‑101 (MQ=255)
           ttGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGgc                                                                                      <  1:1673955/101‑1 (MQ=255)
             gtcgtcACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGgcgc                                                                                    >  1:155193/1‑101 (MQ=255)
             gtcgtcACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGgcgc                                                                                    >  2:1134053/1‑101 (MQ=255)
               cgtcACCGTGCGCGCGGTTAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCg                                                                                  <  2:3122177/101‑1 (MQ=255)
                gtcACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGa                                                                                 >  1:2388036/1‑101 (MQ=255)
                  cgccGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATc                                                                               <  1:3679617/99‑1 (MQ=255)
                  ccccGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATc                                                                               <  2:28513/99‑1 (MQ=255)
                  cACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGCGCAGGTTCGGAAGCCCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATc                                                                               >  2:3852726/1‑101 (MQ=255)
                   aCCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCCAGAACTTTTTCACCCGGCTCAGCCCGATGCGCGGCGCCGGTCt                                                                              >  2:4147200/1‑101 (MQ=255)
                   aCCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCt                                                                              >  1:3600174/1‑101 (MQ=255)
                    ccGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTg                                                                             <  2:3234124/101‑1 (MQ=255)
                       tGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTggcg                                                                          >  2:2472143/1‑101 (MQ=255)
                       tGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTggcg                                                                          >  2:4231941/1‑101 (MQ=255)
                        gcgcgcGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTggcgg                                                                         >  1:3525252/1‑101 (MQ=255)
                         cgcgcgGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTggcggc                                                                        <  1:1564541/101‑1 (MQ=255)
                           cgcgGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGGCt                                                                      <  1:2659795/101‑1 (MQ=255)
                           cgcgGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCt                                                                      <  1:1708666/101‑1 (MQ=255)
                            gcgGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCtt                                                                     >  2:3001450/1‑101 (MQ=255)
                             cgaagAAACCGGCCTATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTc                                                                    <  1:1209909/97‑1 (MQ=255)
                             cgGTGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTc                                                                    <  2:90273/101‑1 (MQ=255)
                              ggTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCt                                                                   >  1:4080691/1‑101 (MQ=255)
                               gTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGCGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTa                                                                  >  1:4001377/1‑101 (MQ=255)
                               gTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTa                                                                  >  2:2617929/1‑101 (MQ=255)
                                tGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTAc                                                                 <  1:2728245/101‑1 (MQ=255)
                                tGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTAc                                                                 >  2:1964885/1‑101 (MQ=255)
                                ggAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTAc                                                                 <  1:3181445/100‑1 (MQ=255)
                                   acccggccgatccggccgatCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGcc                                                              <  2:875250/99‑1 (MQ=255)
                                   aaCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGcc                                                              >  1:2951184/1‑101 (MQ=255)
                                   aaCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGcc                                                              >  1:1085408/1‑101 (MQ=255)
                                      cggccgatccggccgatcCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGt                                                           <  2:2539427/101‑1 (MQ=255)
                                       ggccgaaccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGGTCGGCGCCGATCTGGCGGGCTTCTACGCCCGTg                                                          >  1:4021933/1‑101 (MQ=255)
                                          cgatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCcg                                                       >  1:1297453/1‑101 (MQ=255)
                                              ccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCa                                                   >  1:1006225/1‑101 (MQ=255)
                                               cggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACCCCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCag                                                  <  1:4341480/101‑1 (MQ=255)
                                               cggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCag                                                  <  2:3530015/101‑1 (MQ=255)
                                                ggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGTGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCagg                                                 >  1:3940733/1‑101 (MQ=255)
                                                ggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCagg                                                 <  2:1786662/101‑1 (MQ=255)
                                                     atccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgag                                            <  2:3286853/101‑1 (MQ=255)
                                                      tccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgagg                                           >  1:983334/1‑101 (MQ=255)
                                                         cGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgaggccg                                        <  1:3071885/101‑1 (MQ=255)
                                                           aGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCggg                                      <  1:328116/101‑1 (MQ=255)
                                                           aGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCggg                                      >  2:3601352/1‑101 (MQ=255)
                                                           aGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCggg                                      <  1:521240/101‑1 (MQ=255)
                                                           aGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCggg                                      <  2:3935021/101‑1 (MQ=255)
                                                              tGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATc                                   >  1:3797829/1‑101 (MQ=255)
                                                               ggTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCa                                  <  2:155179/101‑1 (MQ=255)
                                                               ggTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCa                                  >  2:4162661/1‑101 (MQ=255)
                                                                  gCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCAccc                               <  2:524995/101‑1 (MQ=255)
                                                                   cAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCt                              >  2:4665479/1‑101 (MQ=255)
                                                                    aGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTc                             <  2:3897283/101‑1 (MQ=255)
                                                                    aGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTc                             <  1:2083782/101‑1 (MQ=255)
                                                                    aGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTc                             <  2:2507473/101‑1 (MQ=255)
                                                                    aGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTc                             >  1:750102/1‑101 (MQ=255)
                                                                    aGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTc                             <  1:4462608/101‑1 (MQ=255)
                                                                     gCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCg                            <  2:2169228/101‑1 (MQ=255)
                                                                       tGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGAc                          <  2:1376301/101‑1 (MQ=255)
                                                                       tGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGAc                          >  1:653880/1‑101 (MQ=255)
                                                                        gcgcAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACa                         <  1:4614171/101‑1 (MQ=255)
                                                                          gcAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAgc                       >  1:2153391/1‑101 (MQ=255)
                                                                          gcAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAgc                       >  1:3397885/1‑101 (MQ=255)
                                                                           cAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAgcg                      >  2:2132739/1‑101 (MQ=255)
                                                                           cAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAgcg                      >  2:2045888/1‑101 (MQ=255)
                                                                            aaGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGCCAgcgc                     >  2:580703/1‑101 (MQ=255)
                                                                            aaGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAgcgc                     >  2:1357279/1‑101 (MQ=255)
                                                                             aGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGcgcg                    <  2:2387874/101‑1 (MQ=255)
                                                                               aCCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgat                  <  1:4228212/101‑1 (MQ=255)
                                                                               aCCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgat                  <  1:1137475/101‑1 (MQ=255)
                                                                                ccTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgatg                 <  2:4284013/101‑1 (MQ=255)
                                                                                 cTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgatga                >  1:4477917/1‑101 (MQ=255)
                                                                                  tttGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgatgag               >  2:3501732/1‑100 (MQ=255)
                                                                                    tGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCg             >  1:2281862/1‑101 (MQ=255)
                                                                                    tGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCg             >  1:631467/1‑101 (MQ=255)
                                                                                      acacCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACCCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACACCGCGATGATCgtt           >  1:779514/1‑99 (MQ=255)
                                                                                       cgcccGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTg          <  2:3557463/99‑1 (MQ=255)
                                                                                       cacCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTg          <  1:3371609/101‑1 (MQ=255)
                                                                                       cacCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTg          >  2:445251/1‑101 (MQ=255)
                                                                                         cccGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGcc        >  1:3886764/1‑101 (MQ=255)
                                                                                         cccGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGcc        <  2:1797954/101‑1 (MQ=255)
                                                                                          ccGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCg       >  1:2534427/1‑101 (MQ=255)
                                                                                            ggCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGCGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTg     >  1:4177876/1‑101 (MQ=255)
                                                                                             gCTGAGCCCGATGCTCGTCCCGGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGc    <  2:3032414/101‑1 (MQ=255)
                                                                                             gCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGc    <  1:162609/101‑1 (MQ=255)
                                                                                              cTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCa   <  2:3372607/101‑1 (MQ=255)
                                                                                               tGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCaa  >  2:1520402/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CGACAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGATCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCAA  >  NC_014034/2523854‑2524049

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG