Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,531,713 G→C 100% D496E (GAC→GAG RCAP_rcc02362 ← competence protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,531,7130G→C100.0% 773.6 207D496E (GAC→GAGRCAP_rcc02362competence protein
Reads supporting (aligned to +/- strand):  new base (116/91):  ref base (0/0):  total (116/91)

Only 100 of 221 total aligned reads displayed.

ACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAAGTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCACCCCGG  >  NC_014034/2531616‑2531810
                                                                                                  |                                                                                                  
ccAGCCCCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTcg                                                                                                 <  1:4036288/100‑1 (MQ=255)
acggCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTcg                                                                                                 <  2:1155875/98‑1 (MQ=255)
 cAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTcgg                                                                                                <  1:1010181/101‑1 (MQ=255)
    ccccgccgCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTcggcca                                                                                             <  1:104660/99‑1 (MQ=255)
     aCCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTcggccac                                                                                            <  1:3700787/101‑1 (MQ=255)
      ccgccgCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTcggccacg                                                                                           <  1:2385549/101‑1 (MQ=255)
       cgccgcCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTcggccacgg                                                                                          >  1:3507327/1‑101 (MQ=255)
          cgccCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTcggccacgg.cca                                                                                      <  1:1342005/101‑1 (MQ=255)
            ccccGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGc                                                                                    <  2:3206358/101‑1 (MQ=255)
                 gCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCa                                                                               <  2:3624154/101‑1 (MQ=255)
                  ccccAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAg                                                                              <  2:2250426/98‑1 (MQ=255)
                    gCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCCCGGGCAGACAGGTCTGCG.CACCCGCCAGACCCACGACGAACTCGACCACGG.CCAGCACCCAGtg                                                                            >  2:4353869/1‑100 (MQ=255)
                    gCAAGCGCCAGCACCGGCAGCACCCAGCCGGCCGGCGCGGGCAGAAAGGTCGGCG.CCCCCGCCAGACCCGCGACGAACGCGGACACGG.ACAGCACCCAGTc                                                                            >  1:1550882/1‑101 (MQ=255)
                     cAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCg                                                                           >  1:2291376/1‑101 (MQ=255)
                      aaGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCgg                                                                          >  2:3476258/1‑101 (MQ=255)
                       aGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGt                                                                         <  2:1016317/101‑1 (MQ=255)
                            cAGCACCGGCAGCCCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCa                                                                    <  1:2695029/101‑1 (MQ=255)
                            cAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCa                                                                    >  2:4019532/1‑101 (MQ=255)
                             aGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAg                                                                   >  2:1465604/1‑101 (MQ=255)
                             aGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG..CCCCGCCCAACCCCCCAAGGAACCGGGCACGGCCCACCACCCGGTCGG.GCCCAgg                                                                  >  2:4656278/1‑100 (MQ=255)
                              gCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGc                                                                  >  1:3414822/1‑101 (MQ=255)
                               cACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGcc                                                                 <  1:4361320/101‑1 (MQ=255)
                               cACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGcc                                                                 >  1:1440606/1‑101 (MQ=255)
                                aCCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGccc                                                                >  1:3078872/1‑101 (MQ=255)
                                 ccGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGcccc                                                               >  2:2081891/1‑101 (MQ=255)
                                  cGGCAGGACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCa                                                              <  1:766897/101‑1 (MQ=255)
                                    gcagcaCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATc                                                            <  1:794861/101‑1 (MQ=255)
                                     cagccCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCa                                                           <  1:3282760/101‑1 (MQ=255)
                                     caccccccAGCCGGGGCGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCa                                                           <  2:1407028/96‑1 (MQ=255)
                                        caCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGcc                                                        <  1:4589707/101‑1 (MQ=255)
                                         ccccAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCa                                                       <  2:3025227/100‑1 (MQ=255)
                                           ccAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCaga                                                     <  1:4537511/101‑1 (MQ=255)
                                           ccAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCaga                                                     >  2:2827799/1‑101 (MQ=255)
                                            cAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCagag                                                    <  1:2445159/101‑1 (MQ=255)
                                            cAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCagag                                                    >  2:3713413/1‑101 (MQ=255)
                                            cAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCagag                                                    <  1:3597381/101‑1 (MQ=255)
                                            cAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCagag                                                    <  2:32601/101‑1 (MQ=255)
                                            cAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCagag                                                    >  1:4408502/1‑101 (MQ=255)
                                            cAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCagag                                                    <  1:4322789/101‑1 (MQ=255)
                                             aGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAgc                                                   >  2:753861/1‑101 (MQ=255)
                                               ccGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAgcgc                                                 >  2:345265/1‑101 (MQ=255)
                                               ccGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAgcgc                                                 >  1:516960/1‑101 (MQ=255)
                                                cGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGcgcg                                                >  2:3070345/1‑101 (MQ=255)
                                                cGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGcgcg                                                <  2:1862139/101‑1 (MQ=255)
                                                cGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCAGCCAGACCCGCGACGAACTCCGCCACGG.CCAGCACCCAGTCGGTGCCCGGCCCCATCAGCCAGAGcgcg                                                >  1:2632551/1‑101 (MQ=255)
                                                 gggCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCgg                                               >  2:2013584/1‑101 (MQ=255)
                                                 gggCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCgg                                               >  2:2020184/1‑101 (MQ=255)
                                                 gggCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCgg                                               >  2:3132453/1‑101 (MQ=255)
                                                 gggCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCgg                                               <  2:3507069/101‑1 (MQ=255)
                                                 gggCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCgg                                               >  2:737696/1‑101 (MQ=255)
                                                  ggcggcGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCggg                                              >  1:1952709/1‑101 (MQ=255)
                                                   gcggcgCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGc                                             <  1:2405190/101‑1 (MQ=255)
                                                    cggcgCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGcc                                            >  1:726663/1‑101 (MQ=255)
                                                     ggcgCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCAAGTAGGTGCCCAGCCCCATCAGCCAGAGCGCGGGccc                                           >  1:3994671/1‑101 (MQ=255)
                                                      gcgcGGGCGGAAAGGTCTGCC.CCCCCGCCAGACCCGCGACGACCCCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGcccc                                          <  1:1108305/101‑1 (MQ=255)
                                                      gcgcGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGcccc                                          >  2:2508187/1‑101 (MQ=255)
                                                         ggggCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGcc                                       <  2:4468137/100‑1 (MQ=255)
                                                         ggggCAGAAAGG.CCGCGCCCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGcc                                       <  2:2133859/100‑1 (MQ=255)
                                                         cgGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGcc                                       >  2:3761603/1‑101 (MQ=255)
                                                          gggCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCa                                      >  1:2970369/1‑101 (MQ=255)
                                                          gggCAGAAAGGTCGGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCa                                      <  1:2883599/101‑1 (MQ=255)
                                                             cAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGAc                                   >  1:4634442/1‑101 (MQ=255)
                                                              aGAAAGGCCTGCC.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGAcc                                  <  1:3615709/101‑1 (MQ=255)
                                                               gAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGAccc                                 >  2:2426179/1‑101 (MQ=255)
                                                               gAAAGGTCTGCC.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGAccc                                 <  1:3189055/101‑1 (MQ=255)
                                                               gAAAGGTCGGCC.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGAccc                                 <  2:726588/101‑1 (MQ=255)
                                                                aaaGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCa                                >  2:408878/1‑101 (MQ=255)
                                                                aaaGGTCTGCC.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCa                                <  1:321311/101‑1 (MQ=255)
                                                                 aaGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAg                               >  2:237261/1‑101 (MQ=255)
                                                                 aaGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAg                               >  2:246968/1‑101 (MQ=255)
                                                                  aGGTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAgc                              >  1:2781940/1‑101 (MQ=255)
                                                                   ggTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAgcg                             >  1:164634/1‑101 (MQ=255)
                                                                    gTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAgcgg                            >  1:2150370/1‑101 (MQ=255)
                                                                    gTCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAgcgg                            >  2:4621769/1‑101 (MQ=255)
                                                                     tCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAgcggc                           >  1:1158225/1‑101 (MQ=255)
                                                                     tCTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAgcggc                           >  1:3657349/1‑101 (MQ=255)
                                                                      cTGCG.CCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAgcggcg                          >  1:482773/1‑101 (MQ=255)
                                                                        gcg.cCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCCGCGGCGcc                        >  1:1989452/1‑101 (MQ=255)
                                                                          g.cCCCCGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAg                      <  2:3144570/101‑1 (MQ=255)
                                                                            cccccGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGa                     <  2:1950890/101‑1 (MQ=255)
                                                                            cccccGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGa                     >  1:8353/1‑101 (MQ=255)
                                                                            cccccGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGACAGCGGGCCCCGCCAGACCCAGCGGCGCCAGa                     <  1:4151523/101‑1 (MQ=255)
                                                                             ccccGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGaa                    <  1:667397/101‑1 (MQ=255)
                                                                               ccGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAcc                  <  2:1581954/101‑1 (MQ=255)
                                                                                cGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccg                 >  1:1813584/1‑101 (MQ=255)
                                                                                cGCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccg                 <  1:2046764/101‑1 (MQ=255)
                                                                                 gCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccgc                <  1:283894/101‑1 (MQ=255)
                                                                                 gCCAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccgc                >  2:255872/1‑101 (MQ=255)
                                                                                  ccAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccgcc               <  1:3838680/101‑1 (MQ=255)
                                                                                  ccAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccgcc               >  1:2565644/1‑101 (MQ=255)
                                                                                  ccAGACCCGCGACGAACCCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccgcc               <  2:3930652/101‑1 (MQ=255)
                                                                                   cAGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccgccg              >  2:2892806/1‑101 (MQ=255)
                                                                                    aGACCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACcgccgc             >  2:4108643/1‑101 (MQ=255)
                                                                                      aCCCGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCa           <  2:153223/101‑1 (MQ=255)
                                                                                        ccGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGc         >  1:1525969/1‑101 (MQ=255)
                                                                                        ccGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGc         <  2:268565/101‑1 (MQ=255)
                                                                                        ccGCGACGAACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGc         >  2:16701/1‑101 (MQ=255)
                                                                                             acgaACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCAcccc    <  1:3468889/101‑1 (MQ=255)
                                                                                             acgaACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCAcccc    <  2:686057/101‑1 (MQ=255)
                                                                                              cgaACTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCACCCCg   <  2:522768/101‑1 (MQ=255)
                                                                                                  |                                                                                                  
ACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCG.CCCCCGCCAGACCCGCGACGAAGTCGGCCACGG.CCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCACCCCGG  >  NC_014034/2531616‑2531810

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG