Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,549,153 G→A 100% G56S (GGC→AGC)  tonB → protein TonB

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,549,1530G→A100.0% 853.3 226G56S (GGC→AGC) tonBprotein TonB
Reads supporting (aligned to +/- strand):  new base (103/122):  ref base (0/0):  total (104/122)

Only 100 of 246 total aligned reads displayed.

CGCTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACGGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCGA  >  NC_014034/2549055‑2549250
                                                                                                  |                                                                                                 
 gCTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGGGGCGAGGATCTGGACAGcc                                                                                                >  2:4341519/1‑101 (MQ=255)
  cTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCt                                                                                               <  2:4124175/101‑1 (MQ=255)
   tGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTc                                                                                              >  2:4660982/1‑101 (MQ=255)
   tGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTc                                                                                              <  2:1815672/101‑1 (MQ=255)
     gcgcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGa                                                                                            <  1:4363316/101‑1 (MQ=255)
     gcgcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGa                                                                                            <  1:260271/101‑1 (MQ=255)
      cgcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGaa                                                                                           >  1:3032876/1‑101 (MQ=255)
       gcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGCTCTGGACAGCCTCGacg                                                                                          >  1:2293603/1‑99 (MQ=255)
       gcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAg                                                                                          >  2:647540/1‑101 (MQ=255)
       gcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAg                                                                                          <  2:3988606/101‑1 (MQ=255)
        cTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGc                                                                                         >  2:507074/1‑101 (MQ=255)
         tGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCg                                                                                        <  1:3367746/101‑1 (MQ=255)
         tGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGAGCTGGGCAGCCTCGAAGCg                                                                                        >  2:3912976/1‑101 (MQ=255)
            aTGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGCGGATTTGGACAGCCACGCAggtatc                                                                                     <  2:145202/101‑6 (MQ=255)
            aTGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATc                                                                                     >  2:1504928/1‑101 (MQ=255)
             tGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATcg                                                                                    <  1:3611939/101‑1 (MQ=255)
               cGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATcgcc                                                                                  >  2:91404/1‑101 (MQ=255)
                ggTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGGTCTGGACAGCCTCGAAGCGCTccccg                                                                                 >  2:3331722/1‑101 (MQ=255)
                ggTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATcgccg                                                                                 <  2:3032632/101‑1 (MQ=255)
                 gTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATcgccgc                                                                                <  1:2345734/101‑1 (MQ=255)
                     cgGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGAcc                                                                            <  2:2195734/101‑1 (MQ=255)
                      ggTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCt                                                                           >  1:2149533/1‑101 (MQ=255)
                      ggTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCt                                                                           <  2:2514222/101‑1 (MQ=255)
                       gTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCtt                                                                          <  2:3854978/101‑1 (MQ=255)
                         ttCCGTGCTCTCGCAGCCCGAGGCCGCCCCCGCGGCGCTGTTGGCGGCTGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcg                                                                        <  1:3720341/101‑1 (MQ=255)
                         ttCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGGGGGCGGCGAGGATCTGGACAGGCCCGAAGCGATCGGCGCGACCTtcg                                                                        >  2:3008419/1‑101 (MQ=255)
                         ttCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcg                                                                        >  2:1606740/1‑101 (MQ=255)
                          tCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgt                                                                       <  2:142620/101‑1 (MQ=255)
                            cGTGCTCTCGCAGAGCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgtcg                                                                     >  2:2882456/1‑101 (MQ=255)
                            cGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgtcg                                                                     <  1:3201848/101‑1 (MQ=255)
                            cGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgtcg                                                                     >  1:1182031/1‑101 (MQ=255)
                            cGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgtcg                                                                     >  1:4292561/1‑101 (MQ=255)
                            cGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgtcg                                                                     <  2:1850341/101‑1 (MQ=255)
                            cGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgtcg                                                                     <  2:1655714/101‑1 (MQ=255)
                             gTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGa                                                                    <  2:1077960/101‑1 (MQ=255)
                             gTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCCGGCCAGCCTCGAAGCGATCGCCGCGACCTTCGTCGa                                                                    >  1:1990440/1‑101 (MQ=255)
                              tGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCCTCGTCGAt                                                                   >  2:566195/1‑101 (MQ=255)
                                ctctCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCt                                                                 >  2:4534035/1‑101 (MQ=255)
                                ctctCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCt                                                                 <  1:2914734/101‑1 (MQ=255)
                                  ctcGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTgg                                                               >  2:3158194/1‑101 (MQ=255)
                                      cAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgcc                                                           >  1:1663159/1‑101 (MQ=255)
                                      cAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgcc                                                           >  1:2198046/1‑101 (MQ=255)
                                        gACCGAGGCCGGCCCCGCGGCGCTGGTGGCGGCGGGCTCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgccgc                                                         <  2:1269535/101‑1 (MQ=255)
                                        gACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgccgc                                                         >  1:396774/1‑101 (MQ=255)
                                         aCCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCa                                                        >  2:3253450/1‑101 (MQ=255)
                                          ccGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAg                                                       >  2:2663002/1‑101 (MQ=255)
                                          ccGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCCCAg                                                       <  1:3591525/101‑1 (MQ=255)
                                           cGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGa                                                      <  1:1098667/101‑1 (MQ=255)
                                            gAGGCCGGACGCGGGGCGCTGGGGGCGGCTGGTTGGGCCGGCGAGGATCTGGCCAGCGTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGAt                                                     <  1:13689/101‑1 (MQ=255)
                                            gAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGAt                                                     >  1:1393396/1‑101 (MQ=255)
                                            gAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGAt                                                     >  1:2400537/1‑101 (MQ=255)
                                              ggCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATcc                                                   <  2:2409364/101‑1 (MQ=255)
                                               gccacacccGCGGATGTGGTGGCGGCGGGCGCGTGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCTGCGACCTTCGTCGATCTGGCGTCGCAGATCCa                                                  <  1:4029023/95‑1 (MQ=255)
                                                ccgccCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGGTCCAg                                                 >  1:951621/1‑101 (MQ=255)
                                                     cccGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcc                                            <  1:2206660/101‑1 (MQ=255)
                                                     cccGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcc                                            >  2:2771575/1‑101 (MQ=255)
                                                       cgcgGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccg                                          >  2:3613335/1‑101 (MQ=255)
                                                       cgcgGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccg                                          <  2:1916877/101‑1 (MQ=255)
                                                       cgaggcgCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTGGTCGATCTGGCGCCGCAGATCCAGCTgcccg                                          <  1:4171609/98‑1 (MQ=255)
                                                          ggcgCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgacc                                       >  1:1901225/1‑99 (MQ=255)
                                                             gcTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagcccg                                    >  1:1861296/1‑101 (MQ=255)
                                                             gcTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagcccg                                    <  1:3292183/101‑1 (MQ=255)
                                                                ggtggCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGCTCCAGCTGCCCCAGCCCgcga                                 >  1:1611893/1‑101 (MQ=255)
                                                                 gtggCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGAt                                <  2:35842/101‑1 (MQ=255)
                                                                  tggCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATg                               <  1:2831560/101‑1 (MQ=255)
                                                                   ggcggcGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATgg                              <  2:1805523/101‑1 (MQ=255)
                                                                    gcggcgGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGgc                             >  1:263911/1‑101 (MQ=255)
                                                                     cggcggGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGgcg                            <  1:1817401/101‑1 (MQ=255)
                                                                      ggcgggcgCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGgcgc                           >  2:2225101/1‑101 (MQ=255)
                                                                       gcgggcgcgggcGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCt                          <  1:1966561/101‑1 (MQ=255)
                                                                        cgggcgcgggcgGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTg                         >  1:754119/1‑101 (MQ=255)
                                                                         gggcgcgggcgGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGc                        <  1:3773358/101‑1 (MQ=255)
                                                                         gggcgcgggcgGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGc                        >  2:1489008/1‑101 (MQ=255)
                                                                          ggcgcgggcgGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGcc                       >  2:1938351/1‑101 (MQ=255)
                                                                            cgcgggcgGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGc                     >  1:3488846/1‑101 (MQ=255)
                                                                             gcgggcggCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCt                    <  1:4657133/101‑1 (MQ=255)
                                                                             gcgggcggCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCt                    <  2:864587/101‑1 (MQ=255)
                                                                             gcgggcggCGAGGAGCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCt                    <  2:3134035/101‑1 (MQ=255)
                                                                                ggcggcGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGAc                 <  1:4557877/101‑1 (MQ=255)
                                                                                ggcggcGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGAc                 >  1:4223977/1‑101 (MQ=255)
                                                                                  cggcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACcg               >  2:1684580/1‑101 (MQ=255)
                                                                                  cggcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACcg               <  2:800653/101‑1 (MQ=255)
                                                                                    gcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACcgcg             >  1:124943/1‑101 (MQ=255)
                                                                                     cgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCgcgc            <  2:1970641/101‑1 (MQ=255)
                                                                                     cgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCgcgc            >  1:58178/1‑101 (MQ=255)
                                                                                     cgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCgcgc            >  2:422257/1‑101 (MQ=255)
                                                                                      gAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACcgaact           <  2:1287486/101‑5 (MQ=255)
                                                                                      gAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGcc           >  1:34583/1‑101 (MQ=255)
                                                                                      gAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGcc           >  2:2344637/1‑101 (MQ=255)
                                                                                      gAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGcc           >  2:4096628/1‑101 (MQ=255)
                                                                                       aGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCg          >  1:612114/1‑101 (MQ=255)
                                                                                        ggATCTTGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCgg         >  1:2780945/1‑101 (MQ=255)
                                                                                         gATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGt        >  1:1493734/1‑101 (MQ=255)
                                                                                         gATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGt        >  2:548363/1‑101 (MQ=255)
                                                                                          aTCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTg       >  1:4057242/1‑101 (MQ=255)
                                                                                          aTCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTg       >  2:938045/1‑101 (MQ=255)
                                                                                            cTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGAt     <  1:2968494/101‑1 (MQ=255)
                                                                                             tGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATc    >  1:1512206/1‑101 (MQ=255)
                                                                                              ggACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCg   <  1:1758770/101‑1 (MQ=255)
                                                                                              ggACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCg   >  2:1733629/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CGCTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACGGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCGA  >  NC_014034/2549055‑2549250

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG