BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,566,625	C→G	11.2%	intergenic (+97/+97)	purD → / ← sdaA	phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,566,625	0	C→G	11.2%	39.9 ‑3.6 ‑0.0	169	intergenic (+97/+97)	purD/sdaA	phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand): new base (18/1): ref base (105/45): total (123/46)
Fisher's exact test strand distribution p-value = 2.62e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 189 total aligned reads displayed.


AGACGAAAC.GGGGGCGCTGCCCCC.CTCCTG..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAACCGCCTCAG  >  NC_014034/2566528‑2566725


agaCGAAACGGGGGGCGCTGCCCCC.CTCTTGCCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGccc                                                                                                      <  1:2658160/101‑1 (MQ=21)


 gaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCg                                                                                                     <  1:4327812/101‑1 (MQ=21)


 gaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCg                                                                                                     <  1:1430547/101‑1 (MQ=21)


    gAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                                                                                                  <  1:4492940/101‑1 (MQ=21)


      aacggggggCGCTGCCCCC.CTCTTGGCCTTCGGGCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAata                                                                                                <  1:3970909/97‑1 (MQ=25)


      aacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAata                                                                                                <  2:2855141/97‑1 (MQ=25)


      aacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCTTTTCCTTTCATCTTTCCCGAAata                                                                                                >  1:3728697/5‑101 (MQ=21)


       gcagggggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggg                                                                                         >  1:1479095/4‑101 (MQ=0)


       gcagggggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggg                                                                                         >  2:1564774/4‑101 (MQ=0)


       acggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGACTTTTTCTTTCATCTTTCCCGAAaaat                                                                                               >  1:266360/4‑101 (MQ=18)


        cggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATc                                                                                              <  1:2141213/99‑1 (MQ=25)


        cagggggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggg                                                                                        >  1:1296958/3‑101 (MQ=0)


         agggggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggg                                                                                       >  2:2620134/2‑101 (MQ=0)


         agggggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggg                                                                                       >  1:4097678/2‑101 (MQ=0)


         agggggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggg                                                                                       >  2:2229699/2‑101 (MQ=0)


         agggggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCCTGCCGCAAATATCCCggggg                                                                                       >  1:4292733/2‑101 (MQ=2)


          gggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCCCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc                                                                                            <  2:1496807/101‑1 (MQ=25)


           ggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCg                                                                                           <  1:4310501/101‑1 (MQ=25)


            gggCGCTGCCCCCTCTTGGC..CTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggggag                                                                                    >  2:453797/1‑98 (MQ=2)


            gggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCgg                                                                                          <  2:1846739/101‑1 (MQ=37)


            gggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggggag                                                                                    >  2:2471942/1‑98 (MQ=2)


            gggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggggag                                                                                    >  2:548785/1‑98 (MQ=2)


             ggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCggg                                                                                         <  1:1041361/101‑1 (MQ=37)


                 cTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGac                                                                                     >  1:3430278/1‑100 (MQ=37)


                  tGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTTGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTTCCCGAAATATCCCGGGGGAGc                                                                                    >  2:1791967/1‑101 (MQ=25)


                  tGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGc                                                                                    >  1:1858119/1‑101 (MQ=37)


                   gCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGGGATTTTGGGCCAGATAAAGAAGGGCTTTTTCTTTTATCTTTCCCGAAATATTCCGGGGGGGcc                                                                                   >  1:4652424/1‑101 (MQ=16)


                        ccctcttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCt                                                                               >  2:3387675/11‑101 (MQ=255)


                           tcttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgc                                                                            <  2:3659272/94‑1 (MQ=255)


                           tcttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgc                                                                            >  2:311734/8‑101 (MQ=255)


                            cttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcg                                                                           >  2:2390671/7‑101 (MQ=255)


                             ttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcgg                                                                          >  2:145181/6‑101 (MQ=255)


                             ttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcgg                                                                          >  1:1260221/6‑101 (MQ=255)


                                  cTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGcggggggg                                                                     >  1:4083907/1‑101 (MQ=255)


                                   ttCGGCCAATTCCCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCgggg                                                                    <  1:3789572/101‑1 (MQ=255)


                                     cGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGc                                                                  >  2:4334528/1‑101 (MQ=255)


                                     cGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGc                                                                  >  2:3071813/1‑101 (MQ=255)


                                     cGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGc                                                                  >  2:2879579/1‑101 (MQ=255)


                                     cGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGc                                                                  >  1:3572051/1‑101 (MQ=255)


                                     cGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGc                                                                  <  2:4470275/101‑1 (MQ=255)


                                     cGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCgggggg                                                                  >  2:112100/1‑100 (MQ=255)


                                      ggCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCa                                                                 >  2:4398588/1‑101 (MQ=255)


                                      ggCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCa                                                                 >  2:2112354/1‑101 (MQ=255)


                                       gCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCgggggggg                                                                >  1:889183/1‑98 (MQ=255)


                                        ccAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAgc                                                               <  1:2470017/101‑1 (MQ=255)


                                           aTTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGCGCCCGCTTGCGGGCGGGGGCCGCGcc                                                            >  1:75798/1‑101 (MQ=255)


                                               cccccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCg                                                        <  2:2529228/100‑1 (MQ=255)


                                                 cccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGGGGCGCCCCCGaa                                                      >  1:871839/1‑101 (MQ=255)


                                                     cGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAgcgc                                                  >  1:3907795/1‑101 (MQ=255)


                                                      gggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGcc                                                 >  2:2052368/1‑101 (MQ=255)


                                                      gggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGcc                                                 >  1:3653583/1‑101 (MQ=255)


                                                        gATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCg                                               <  2:1156215/101‑1 (MQ=255)


                                                        gATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCg                                               >  2:2206216/1‑101 (MQ=255)


                                                         atatTTCGGGCAAGATAAAGAAGGCCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGCGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCTGAAGCGCCCGa                                              >  2:2298931/1‑101 (MQ=255)


                                                          tatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                                             >  2:414178/1‑101 (MQ=255)


                                                               cGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGACCGAcgcgcg                                        >  1:3268749/1‑101 (MQ=255)


                                                                gggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcgg                                       >  2:3731532/1‑101 (MQ=255)


                                                                  gCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggcg                                     >  1:1529398/1‑101 (MQ=255)


                                                                  gCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggcg                                     >  1:2564334/1‑101 (MQ=255)


                                                                   cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg                                    >  1:4199385/1‑101 (MQ=255)


                                                                   cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg                                    >  1:3087612/1‑101 (MQ=255)


                                                                    gatctAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg                                   <  2:3863356/97‑1 (MQ=255)


                                                                       aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt                                <  1:4088302/101‑1 (MQ=255)


                                                                       aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt                                <  2:2958731/101‑1 (MQ=255)


                                                                         aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc                              >  1:1038036/1‑101 (MQ=255)


                                                                         aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc                              >  1:1772266/1‑101 (MQ=255)


                                                                         aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc                              >  1:1963933/1‑101 (MQ=255)


                                                                         aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGAGCCCCCGAAGCACCCGACGCGCGGCGGGCGTgc                              >  1:3595745/1‑101 (MQ=255)


                                                                         aaaGAAGGTCTGTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc                              <  2:75792/101‑1 (MQ=255)


                                                                          aagaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcc                             >  1:3666247/1‑101 (MQ=255)


                                                                           agaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGGGGCGGGCTTgccc                            >  2:3031894/1‑101 (MQ=255)


                                                                           agaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgccc                            <  2:1700448/101‑1 (MQ=255)


                                                                            gaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGCAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccg                           >  2:4105022/1‑101 (MQ=255)


                                                                               ggTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgccc                        >  2:725416/1‑101 (MQ=255)


                                                                                gTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCa                       >  2:1607687/1‑101 (MQ=255)


                                                                                 tCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCaa                      >  1:929083/1‑101 (MQ=255)


                                                                                 tCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCaa                      <  1:1792086/101‑1 (MQ=255)


                                                                                 tCTTTCCCTTTCATCTGGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCaa                      <  2:712653/101‑1 (MQ=255)


                                                                                  cTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAag                     <  2:3475991/101‑1 (MQ=255)


                                                                                  cTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAag                     >  1:3568872/1‑101 (MQ=255)


                                                                                   ttttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagg                    >  1:2637512/1‑101 (MQ=255)


                                                                                    tttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagga                   >  1:877834/1‑101 (MQ=255)


                                                                                    tttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagga                   >  1:302387/1‑101 (MQ=255)


                                                                                       ccTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCTGGCTTGCCCGCCCAAGGAggg                >  2:4595716/1‑101 (MQ=255)


                                                                                       ccTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAggg                >  1:2751832/1‑101 (MQ=255)


                                                                                        cTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgc               >  2:1721572/1‑101 (MQ=255)


                                                                                        cTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgc               <  2:1857993/101‑1 (MQ=255)


                                                                                         tttCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgcg              >  2:3919832/1‑101 (MQ=255)


                                                                                         tttCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgcg              >  1:720668/1‑101 (MQ=255)


                                                                                         tttCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgcg              >  2:2354950/1‑101 (MQ=255)


                                                                                          ttCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgcgc             >  1:1586826/1‑101 (MQ=255)


                                                                                          ttCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgcgc             >  1:2876540/1‑101 (MQ=255)


                                                                                            cagcTTGCCCGAAATACCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCaa           <  1:2052508/98‑1 (MQ=255)


                                                                                              tCTTGCCCAAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAAcc         <  2:4083621/101‑1 (MQ=255)


                                                                                                 tccccGAAATATCCCGGGGGAGCCCGCTCGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAACCGcc      <  1:1071456/99‑1 (MQ=255)


                                                                                                 tGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAACCGcc      >  2:2553000/1‑101 (MQ=255)


                                                                                                   cgcGAAATATCCCGGGGGAGCCCGATTGCGGGCGGGGGCCGCCCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAACCGCCTc    <  2:512725/99‑1 (MQ=255)


                                                                                                    ccGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAACCGCCTca   <  1:768580/101‑1 (MQ=255)


                                                                                                    ccGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAACCGCCTca   >  1:3479519/1‑101 (MQ=255)


                                                                                                     cGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAACCGCCTcag  <  2:2564160/101‑1 (MQ=255)


AGACGAAAC.GGGGGCGCTGCCCCC.CTCCTG..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAACCGCCTCAG  >  NC_014034/2566528‑2566725

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG