Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 257,090 C→G 100% intergenic (+24/‑175) secA → / → RCAP_rcc00221 preprotein translocase subunit SecA/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034257,0900C→G100.0% 695.3 186intergenic (+24/‑175)secA/RCAP_rcc00221preprotein translocase subunit SecA/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (91/95):  ref base (0/0):  total (91/95)

Only 100 of 210 total aligned reads displayed.

GAGCCCTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GCCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCGG  >  NC_014034/256992‑257187
                                                                                                   |                                                                                                  
       cgcgCAATGCCCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.ggg                                                                                          <  1:4527019/101‑1 (MQ=255)
         cgcAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGt                                                                                        <  1:2209916/101‑1 (MQ=255)
           cAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGGGCC.ggggggg                                                                                      >  1:2800090/1‑98 (MQ=255)
             aTGCCCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAAc                                                                                    <  1:3773746/101‑1 (MQ=255)
             aTGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAAc                                                                                    <  1:2715921/101‑1 (MQ=255)
              tgcccccggcccctgCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAAcc                                                                                   <  2:2176523/98‑1 (MQ=255)
              tGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAAcc                                                                                   <  2:3577971/101‑1 (MQ=255)
               gACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGGGCC.GGGGGGGAccc                                                                                  >  2:2349625/1‑101 (MQ=255)
                 cccctgcccctgCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCgg                                                                                <  1:1221014/101‑1 (MQ=255)
                 cccctgcccctgCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.CGGGGGAGccccg                                                                                >  1:3055257/1‑99 (MQ=255)
                  ccctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCggg                                                                               <  1:3842577/101‑1 (MQ=255)
                   cctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGgc                                                                              <  1:4577527/101‑1 (MQ=255)
                       cccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCCCGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGGGGC.AGGGGGGACCCGGggcgcg                                                                          >  2:436086/1‑100 (MQ=255)
                        ccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGccgccg                                                                         <  1:599907/101‑1 (MQ=255)
                         cctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGGTCC.GGGGGGAGCCGgggggcccg                                                                         >  1:2359722/1‑95 (MQ=255)
                          ctgcGGCTCGGGCGAGAAGTTCAAGCCCTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGACCCCGGGCCGCCGtt                                                                       <  1:4658428/101‑1 (MQ=255)
                           tgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGGGCCGGGGGGGAACCCGGGCGCCCGtt                                                                       >  2:107657/1‑101 (MQ=255)
                           tgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAACCCGGGCCGCCCttt                                                                      >  1:1622332/1‑101 (MQ=255)
                             cGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGGACCCCGGGCGCCGgtttt                                                                    >  1:1407216/1‑101 (MQ=255)
                              ggCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAACCCGGGCCGCCGGTTTTc                                                                   >  1:893381/1‑101 (MQ=255)
                               gCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGGGCC.GGGGGGGAACCCGGCCGCCCCTTTTcc                                                                  >  2:1625519/1‑100 (MQ=255)
                                  cGGGCGAGAAGTTCAAGCACTGCCACGGCCGTGTGGCGTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTa                                                               <  2:4002572/101‑1 (MQ=255)
                                  cGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTa                                                               <  1:1890481/101‑1 (MQ=255)
                                  cGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAACCCGGGCCGCCGgtttttctta                                                               >  1:3024830/1‑96 (MQ=255)
                                    ggCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATc                                                             <  1:4448416/101‑1 (MQ=255)
                                      ggagaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCt                                                           <  1:2840868/100‑1 (MQ=255)
                                      cGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCCGTGCC.GGAGGTACCCCCGGCC.CCGCCTTTCATTTTCCt                                                           >  2:1839467/1‑101 (MQ=255)
                                      cGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCt                                                           <  1:2502719/101‑1 (MQ=255)
                                      cGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCt                                                           <  1:4128561/101‑1 (MQ=255)
                                      cGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCt                                                           <  2:4651123/101‑1 (MQ=255)
                                       gagaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTg                                                          <  1:789069/101‑1 (MQ=255)
                                        agaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGt                                                         <  1:2039848/101‑1 (MQ=255)
                                        agaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGGACCCGGGCCGCCGGTTTTTATTATCCTGt                                                         >  2:3796548/1‑101 (MQ=255)
                                         gaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGGACCCGGGGCGCCGGTTTTCATTATCCTTtt                                                        >  1:1098039/1‑101 (MQ=255)
                                         gaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAAACCGGGCCCCCGTTTTTCATTTGCCTGtt                                                        >  1:761855/1‑101 (MQ=255)
                                          aaGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTg                                                       <  2:2643498/101‑1 (MQ=255)
                                            gTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATCTGGG.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGAt                                                     <  1:1839593/101‑1 (MQ=255)
                                              tCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAACCCGGGGCGCCGTTTTTTATTTTCCTCTTgtttt                                                   >  1:2244505/1‑97 (MQ=255)
                                              tCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.CGGGGGAAACCGGGGCGCCGTTTTTCATTATCCTCTTGATCt                                                   >  2:1753686/1‑101 (MQ=255)
                                              tCAAGCACTGCCACGGCCGTCGGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCt                                                   <  2:3273139/101‑1 (MQ=255)
                                               cAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTg                                                  <  1:4294452/101‑1 (MQ=255)
                                                aaGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTgg                                                 <  2:3137894/101‑1 (MQ=255)
                                                aaGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTgg                                                 <  1:1080311/101‑1 (MQ=255)
                                                 aGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGGACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGa                                                >  1:3436733/1‑101 (MQ=255)
                                                 aGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAACCCGGGCCGCCGGTTTTTATTATCCTGTTTGTCTggg                                                >  1:3368354/1‑100 (MQ=255)
                                                 aGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAACCCGGGCCGCCGTTTTTTATTATCCTGTTGATCTGGa                                                >  1:4406379/1‑101 (MQ=255)
                                                    aCTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGatat                                             <  1:984404/101‑1 (MQ=255)
                                                    aCTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAACCCGGGCCGCCGGTTTTCATTATCCTGTTGATCTGGatat                                             >  2:4659780/1‑101 (MQ=255)
                                                      tGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGt                                           <  2:200230/101‑1 (MQ=255)
                                                       gCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGACCCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGtt                                          <  2:733623/101‑1 (MQ=255)
                                                       gCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGtt                                          <  1:1666609/101‑1 (MQ=255)
                                                       gCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAACCCGGGGCGCCGGTTTTTATTTTTCTTTTTGTCTGGGTTTGtt                                          >  1:3536329/1‑101 (MQ=255)
                                                       gCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAACCCCGGGCGCCGTTTTTCATTATCCTGTTGTTCTTGGTATGtt                                          >  1:3965453/1‑101 (MQ=255)
                                                        ccACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGttt                                         <  2:142542/101‑1 (MQ=255)
                                                        ccACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAACCCGGGGCCCCGGTTTTCATTATCCTGTTGATCTGGGTATGttt                                         >  1:66998/1‑101 (MQ=255)
                                                        ccACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCCGTCC.GGGGGGGACCCGGGCCCCCGCTTTTTCTTATCCTGTTGATTTTGCTTTGttt                                         >  1:4437570/1‑101 (MQ=255)
                                                          aCGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAACCCGGGGCGCCGTTTTTCATTATCCTGTTGATCTGGATATGttttt                                       >  1:494791/1‑101 (MQ=255)
                                                           cGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTtg                                      <  2:2799874/101‑1 (MQ=255)
                                                           cGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGATCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGtttttt                                      >  1:2320896/1‑100 (MQ=255)
                                                            ggCCGCCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGACCCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTtgt                                     <  1:2565855/101‑1 (MQ=255)
                                                                gTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGGGCC.GGGGGGGACCCGGGCCGCCGCTTTTCATTATTCTGTTGATCTGGATATGTTTTTGTggga                                 >  2:3874444/1‑99 (MQ=255)
                                                                gTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGaa                                 <  1:1504494/101‑1 (MQ=255)
                                                                gTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGaa                                 <  1:4226078/101‑1 (MQ=255)
                                                                gTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGGTATGTTTTTTTggga                                 >  2:2614949/1‑99 (MQ=255)
                                                                  cTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAAt                               <  2:1655774/101‑1 (MQ=255)
                                                                   tGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAAtt                              <  2:609897/101‑1 (MQ=255)
                                                                   tGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAAACCGGGCCGCCGCTTTTCATTATCCTGTTGGTCTGGATATGTTTTTTTGGAAAtt                              >  2:40896/1‑101 (MQ=255)
                                                                    ggCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAAttt                             <  2:4206697/101‑1 (MQ=255)
                                                                    ggCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAAttt                             <  2:913048/101‑1 (MQ=255)
                                                                    ggCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAAttt                             <  1:1387691/101‑1 (MQ=255)
                                                                    ggCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAAttt                             <  1:1753805/101‑1 (MQ=255)
                                                                    ggCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAACCCCGGCCGCCGGTTTTCCTTATCCTGTTGATCTGGATATGTTTTTTTGGAAAttt                             >  1:1253277/1‑101 (MQ=255)
                                                                     gCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTTTGGGAATTTc                            >  2:3535243/1‑101 (MQ=255)
                                                                      ccTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGCACCCCGGCCGCCCGTTTTCCTTATCCCGTTGGTCTGGGTATGTTTTTGTGGgaaataga                           >  2:2279216/1‑96 (MQ=255)
                                                                      ccTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTTTGGAAAttttc                           >  2:3475264/1‑99 (MQ=255)
                                                                       cTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTccg                          <  2:1284411/101‑1 (MQ=255)
                                                                       cTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTccg                          <  1:3115363/101‑1 (MQ=255)
                                                                       cTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAACCCGGGCCGCCGTTTTTTATTATCCTGTTGATCTGGGTATGTTTTTTTGGGAATTTccg                          >  1:2947744/1‑101 (MQ=255)
                                                                          aaaCAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTTTGGAAATTTccggcc                       >  2:2921813/1‑101 (MQ=255)
                                                                          aaaCAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTccggcc                       >  1:1442036/1‑101 (MQ=255)
                                                                           aaCAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTccggccg                      <  2:4129734/101‑1 (MQ=255)
                                                                            aCAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTccggccgg                     <  1:1803837/101‑1 (MQ=255)
                                                                              aaaGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTTCGGGCGGGc                   >  2:4460553/1‑101 (MQ=255)
                                                                               aaGGCGGTGAAATATGGG.GGCGGTCC.GGGGGGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCg                  <  1:974820/101‑1 (MQ=255)
                                                                               aaGGCGCTGAAATATGGC.GGGGGACC.GGGGGGAACCCGGGGCGCCGTTTTTCCTTTTCCTGTTGATCTGGATATGTTTTTGTGGAAATTTACGGCCGGGCg                  >  2:549154/1‑101 (MQ=255)
                                                                                aGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGa                 <  2:1930312/101‑1 (MQ=255)
                                                                                    gcTGAAATATGGC.GGCGGTCC.GGGGGGAACCCGGGCCGCCGTTTTTTATTTTCCTGTTGATCTTGATATGTTTTTGTGGAAATTTCCAGCCGGGCGAAAGt             >  1:1969030/1‑101 (MQ=255)
                                                                                    gcTGAAATAGGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGt             <  2:494743/101‑1 (MQ=255)
                                                                                     cTGAAATATGGC.GGCCGTCC.GGGGTGAAACCCGGCCGCCGTTTTTCATTATCCCGTTGGTTTGGATAAGTTTTTCTGGCAATTTCCCGCCCGGCGGAgggt            >  2:3383845/1‑99 (MQ=255)
                                                                                      tGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGttt           <  2:3965168/101‑1 (MQ=255)
                                                                                      tGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGttt           <  1:4660081/101‑1 (MQ=255)
                                                                                      tGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGGTTTTCATTAAACTGTTGATCTGGAAATGTTTTTGTGGGAATTTTCGGCCTGGCGAAGCttt           >  2:2037896/1‑101 (MQ=255)
                                                                                       gAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTc          <  2:775545/101‑1 (MQ=255)
                                                                                          atatGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGt       <  1:3196290/101‑1 (MQ=255)
                                                                                           tatGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGtt      <  2:1354619/101‑1 (MQ=255)
                                                                                           tatGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGtt      <  2:3311194/101‑1 (MQ=255)
                                                                                            atGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGttt     >  2:4163854/1‑101 (MQ=255)
                                                                                             tGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTc    >  2:468320/1‑101 (MQ=255)
                                                                                               gc.ggCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCgg  <  1:4141642/101‑1 (MQ=255)
                                                                                               gc.gcCGGCGC.GGGGAGAACCCAGGGCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGGTTCCGTTTCgg  <  2:1219491/101‑1 (MQ=255)
                                                                                                   |                                                                                                  
GAGCCCTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GCCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCGG  >  NC_014034/256992‑257187

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG