Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,575,144 G→A 100% G220D (GGC→GAC)  degP → protease Do

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,575,1440G→A100.0% 967.4 245G220D (GGC→GAC) degPprotease Do
Reads supporting (aligned to +/- strand):  new base (112/133):  ref base (0/0):  total (112/133)

Only 100 of 251 total aligned reads displayed.

CGACTGGGTGATGGCGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGGCGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAGGTGATCGGGGTGAACACCGCGATT  >  NC_014034/2575046‑2575241
                                                                                                  |                                                                                                 
cGACTGGGTGATGGCGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACg                                                                                                 >  1:431749/1‑101 (MQ=255)
 gACTGGGTGATGGCGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGa                                                                                                >  2:2114102/1‑101 (MQ=255)
 gACTGGGTGATGGCGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGa                                                                                                <  1:3692429/101‑1 (MQ=255)
 gACTGGGTGATGGCGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGa                                                                                                >  1:4229625/1‑101 (MQ=255)
  aCTGGGTGATGGCGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGAc                                                                                               <  1:3114702/101‑1 (MQ=255)
  aCTGGGTGATGGCGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGAc                                                                                               >  1:1043297/1‑101 (MQ=255)
  aCTGGGTGATGGCGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGAc                                                                                               <  2:391294/101‑1 (MQ=255)
   cTGGGTGATGGCGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACt                                                                                              <  2:3116983/101‑1 (MQ=255)
    tGGGTGATGGCGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTa                                                                                             <  1:1286240/101‑1 (MQ=255)
       gTGATGGCGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACAt                                                                                          <  2:385666/101‑1 (MQ=255)
         gATGGCGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATcc                                                                                        >  1:3803045/1‑101 (MQ=255)
          aTGGCGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGCAACTGAGCGGCACCTATGACGACTACATCCa                                                                                       >  2:3437356/1‑101 (MQ=255)
           tGGCGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAg                                                                                      <  2:2610912/101‑1 (MQ=255)
           tGGCGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAg                                                                                      <  2:1023212/101‑1 (MQ=255)
           tGGCGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAg                                                                                      <  2:1531917/101‑1 (MQ=255)
            ggCGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGCCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGAGGACCAGAGCCAGa                                                                                     >  1:3595957/1‑101 (MQ=255)
              cGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGAcc                                                                                   <  2:791159/101‑1 (MQ=255)
              cGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGAcc                                                                                   <  2:4199498/101‑1 (MQ=255)
                  gggCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATg                                                                               <  1:4488312/101‑1 (MQ=255)
                   ggCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGc                                                                              <  1:407751/101‑1 (MQ=255)
                   ggCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGc                                                                              <  2:2539070/101‑1 (MQ=255)
                   ggCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGc                                                                              <  1:2589093/101‑1 (MQ=255)
                    gCACCCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGcc                                                                             <  2:4486953/101‑1 (MQ=255)
                    gCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGcc                                                                             <  2:2544882/101‑1 (MQ=255)
                     cAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCcg                                                                            >  1:3828282/1‑101 (MQ=255)
                     cAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCcg                                                                            >  2:3663554/1‑101 (MQ=255)
                         ccGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGCCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGAt                                                                        <  1:3937945/101‑1 (MQ=255)
                          cGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATc                                                                       <  2:2133748/101‑1 (MQ=255)
                            cTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCaa                                                                     >  1:2761210/1‑101 (MQ=255)
                            cTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCaa                                                                     <  2:2911966/101‑1 (MQ=255)
                             tGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAAc                                                                    >  1:4539361/1‑101 (MQ=255)
                             tGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAAc                                                                    >  2:2816574/1‑101 (MQ=255)
                                gTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACcgc                                                                 <  2:403963/101‑1 (MQ=255)
                                gTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACcgc                                                                 <  1:2348841/101‑1 (MQ=255)
                                gTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACcgc                                                                 <  1:2875910/101‑1 (MQ=255)
                                   aGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGc                                                              >  2:1364127/1‑101 (MQ=255)
                                     ggCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCaa                                                            >  2:2051662/1‑101 (MQ=255)
                                      gCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAAc                                                           >  2:921613/1‑101 (MQ=255)
                                      gCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAAc                                                           <  1:2891589/101‑1 (MQ=255)
                                      gCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAAc                                                           <  1:3215521/101‑1 (MQ=255)
                                        ttCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTc                                                         <  2:2722279/101‑1 (MQ=255)
                                         tctcGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTcg                                                        <  1:4180056/101‑1 (MQ=255)
                                         tctcGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTcg                                                        <  1:17671/101‑1 (MQ=255)
                                         tctcGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTcg                                                        <  1:2472246/101‑1 (MQ=255)
                                         tctcGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTcg                                                        >  2:1643442/1‑101 (MQ=255)
                                           tcGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTcggg                                                      >  1:1486718/1‑101 (MQ=255)
                                            cGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTcgggc                                                     <  1:2065276/101‑1 (MQ=255)
                                             gACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTcgggcg                                                    >  1:1231243/1‑101 (MQ=255)
                                              aCCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTcgggcgg                                                   >  2:2033734/1‑101 (MQ=255)
                                               ccTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTcgggcggg                                                  <  2:1727338/101‑1 (MQ=255)
                                                 tcggtcggTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGcc                                                <  1:2305347/101‑1 (MQ=255)
                                                 tcggtcggTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGcc                                                <  1:3146368/101‑1 (MQ=255)
                                                 tcggtcggTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGcc                                                >  1:3591049/1‑101 (MQ=255)
                                                  cggtcggtATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGccc                                               >  1:2174401/1‑101 (MQ=255)
                                                  cggtcggtATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGccc                                               >  1:3893663/1‑101 (MQ=255)
                                                   ggtcggtATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCt                                              >  2:2288303/1‑101 (MQ=255)
                                                   ggtcggtATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCt                                              >  2:1896975/1‑101 (MQ=255)
                                                   ggtcggtATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCt                                              <  1:4118491/101‑1 (MQ=255)
                                                    gtcgttATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCtt                                             <  2:1915832/101‑1 (MQ=255)
                                                    gtcggtATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCtt                                             <  2:2786079/101‑1 (MQ=255)
                                                    gtcggtATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCtt                                             <  1:420679/101‑1 (MQ=255)
                                                     tcggtATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTg                                            <  1:194388/101‑1 (MQ=255)
                                                      cggtATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGt                                           <  1:1027031/101‑1 (MQ=255)
                                                         tATCGCCTCGGCGCGCAACCGGGAACTGAGCTGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCa                                        <  2:4666315/101‑1 (MQ=255)
                                                          aTCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAg                                       >  1:677191/1‑101 (MQ=255)
                                                          aTCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAg                                       >  2:4451963/1‑101 (MQ=255)
                                                           tCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGc                                      <  2:4380786/101‑1 (MQ=255)
                                                             gTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCAt                                    >  1:835754/1‑101 (MQ=255)
                                                              tctcGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATg                                   <  2:4505857/101‑1 (MQ=255)
                                                               ctcGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATgg                                  >  2:3019544/1‑101 (MQ=255)
                                                                 cGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGAc                                <  1:3064787/101‑1 (MQ=255)
                                                                 cGGCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGAc                                <  2:2962021/101‑1 (MQ=255)
                                                                  ggCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACg                               <  2:4236268/101‑1 (MQ=255)
                                                                  ggCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACg                               >  1:1413532/1‑101 (MQ=255)
                                                                  ggCGCGCAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACg                               <  2:2392849/101‑1 (MQ=255)
                                                                   gcgcgcAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACgg                              <  1:2288465/101‑1 (MQ=255)
                                                                     gcgcAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCa                            >  1:525058/1‑101 (MQ=255)
                                                                     gcgcAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCa                            <  2:84038/101‑1 (MQ=255)
                                                                      cgcAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCaa                           <  1:2808114/101‑1 (MQ=255)
                                                                      cgcAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCaa                           >  2:3236181/1‑101 (MQ=255)
                                                                        cAACCGGGAACTGAGCGGCACCTATGACGACTGCATCCAGACCGATGCCGCGATCAACCGCGGCAGCTCGGGCGGGCCCTTGTTCACCATGGGCGGCAAgg                         <  2:4597177/101‑1 (MQ=255)
                                                                        cAACCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAgg                         >  2:4305179/1‑101 (MQ=255)
                                                                         aaCCGGGAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAGGt                        <  2:1131693/101‑1 (MQ=255)
                                                                         aaCCGGGAACTGACCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCGAggg                        >  2:627420/1‑100 (MQ=255)
                                                                             gggAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAGGTGATc                    >  1:3307887/1‑101 (MQ=255)
                                                                              ggAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAGGTGATCg                   >  2:2452506/1‑101 (MQ=255)
                                                                               gAACTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAGGTGATCgg                  >  1:1383735/1‑101 (MQ=255)
                                                                                aaCTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAGGTGATCggg                 >  1:3709415/1‑101 (MQ=255)
                                                                                aaCTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAGGTGATCggg                 <  1:914288/101‑1 (MQ=255)
                                                                                  cTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAGGTGATCGGGGt               >  2:2286931/1‑101 (MQ=255)
                                                                                  cTGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAGGTGATCGGGGt               >  2:669704/1‑101 (MQ=255)
                                                                                   tGAGCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAGGTGATCGGGGTg              >  2:781663/1‑101 (MQ=255)
                                                                                      gCGGCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAGGTGATCGGGGTGAac           <  2:4334485/101‑1 (MQ=255)
                                                                                        ggCACCTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAGGTGATCGGGGTGAacac         <  1:4661485/101‑1 (MQ=255)
                                                                                            ccTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAGGTGATCGGGGTGAACACcgcg     >  2:729596/1‑101 (MQ=255)
                                                                                             cTATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAGGTGATCGGGGTGAACACCGCGa    >  1:2098713/1‑101 (MQ=255)
                                                                                              tATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAGGTGATCGGGGTGAACAGCGCGAt   >  1:771096/1‑101 (MQ=255)
                                                                                              tATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAGGTGATCGGGGTGAACACCGCGAt   >  1:1621022/1‑101 (MQ=255)
                                                                                              tATGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAGGTGATCGGGGTGAACACCGCGAt   >  1:3337956/1‑101 (MQ=255)
                                                                                               aTGACGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAGGTGATCNGGGTGAACACCGCGAtt  >  2:1709475/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CGACTGGGTGATGGCGATGGGCAACCCGCTGGGTCAGGGCTTCTCGACCTCGGTCGGTATCGTCTCGGCGCGCAACCGGGAACTGAGCGGCACCTATGGCGACTACATCCAGACCGATGCCGCGATCAACCGCGGCAACTCGGGCGGGCCCTTGTTCAGCATGGACGGCAAGGTGATCGGGGTGAACACCGCGATT  >  NC_014034/2575046‑2575241

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG