BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,579,493	A→C	100%	S227A (TCC→GCC)	pgi ←	glucose‑6‑phosphate isomerase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,579,493	0	A→C	100.0%	704.7	190	S227A (TCC→GCC)	pgi	glucose‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand): new base (94/96): ref base (0/0): total (94/96)

Only 100 of 206 total aligned reads displayed.


GACCGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGAGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTGGACGCC  >  NC_014034/2579395‑2579590


gACCGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGgcga                                                                                                 >  1:3727301/1‑101 (MQ=255)


gACCGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGgcga                                                                                                 >  1:4052661/1‑101 (MQ=255)


 aCCGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGaa                                                                                                >  1:60354/1‑101 (MQ=255)


 aCCGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGaa                                                                                                >  2:1452158/1‑101 (MQ=255)


  ccGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGCGATCGCGgcgact                                                                                               >  2:3895244/1‑99 (MQ=255)


   cGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCAGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAAtt                                                                                              >  1:3937196/1‑101 (MQ=255)


    gAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTg                                                                                             <  1:3562986/101‑1 (MQ=255)


    gAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTg                                                                                             <  2:2705062/101‑1 (MQ=255)


    gAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTg                                                                                             >  1:1877713/1‑101 (MQ=255)


     aaTAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTgc                                                                                            >  2:3153233/1‑101 (MQ=255)


     aaTAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTgc                                                                                            >  1:48772/1‑101 (MQ=255)


       tAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTgcgc                                                                                          >  1:1964909/1‑101 (MQ=255)


        aGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGcgcc                                                                                         >  1:2437006/1‑101 (MQ=255)


          cgcccgccCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGcgccgc                                                                                       <  1:2134864/101‑1 (MQ=255)


           gcccgcccACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCgccgcc                                                                                      <  1:3673641/101‑1 (MQ=255)


            cccgcccACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGCTGCCGAAAGCCCCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGccgacg                                                                                     >  2:4571408/1‑101 (MQ=255)


              cgcccACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCggg                                                                                   >  2:568049/1‑101 (MQ=255)


              cgcccACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCggg                                                                                   >  1:1492992/1‑101 (MQ=255)


                 ccacctaATCCTCGAAGCCGAAGGCGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTcc                                                                                <  2:1500989/101‑1 (MQ=255)


                 ccacccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTcc                                                                                <  2:2990117/101‑1 (MQ=255)


                 ccacccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTcc                                                                                >  2:3296157/1‑101 (MQ=255)


                  cacccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCg                                                                               >  2:2395681/1‑101 (MQ=255)


                  cacccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCg                                                                               <  1:2683460/101‑1 (MQ=255)


                   acccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGt                                                                              >  1:949494/1‑101 (MQ=255)


                    cccaAGCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTg                                                                             <  1:3157239/101‑1 (MQ=255)


                     ccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGa                                                                            <  1:4472588/101‑1 (MQ=255)


                        aTCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCt                                                                         >  2:446479/1‑101 (MQ=255)


                         tCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCtt                                                                        <  1:464879/101‑1 (MQ=255)


                           cTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcg                                                                      >  2:3505293/1‑101 (MQ=255)


                            ccGAGGCCGAAGACGCCCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcgc                                                                     <  2:3322884/100‑1 (MQ=255)


                             cGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcgcc                                                                    <  1:2993188/101‑1 (MQ=255)


                               aaGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcgccgc                                                                  >  1:3479005/1‑101 (MQ=255)


                                  tctaaGACGCTCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCAt                                                               <  2:226325/98‑1 (MQ=255)


                                  ccGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCAt                                                               <  2:3443809/101‑1 (MQ=255)


                                   cGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATc                                                              <  2:3066011/101‑1 (MQ=255)


                                     aaGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCAtaga                                                            >  1:226349/1‑98 (MQ=255)


                                     aaGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCa                                                            >  2:514958/1‑101 (MQ=255)


                                      aGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAg                                                           >  1:1678320/1‑101 (MQ=255)


                                       gACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAgg                                                          <  1:1383666/101‑1 (MQ=255)


                                        aCGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGTTCGCGGCGAATTGCGCCTCCGGGTCCGTGACCTTCGCCGCCATCCAGgc                                                         <  1:4382226/101‑1 (MQ=255)


                                        aCGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGgc                                                         <  1:3640575/101‑1 (MQ=255)


                                         cgcgCTCGGGGGGGATGCCGAAAGCCGCCGTCCTGTCGTTCGCGGTCGAGATCGGGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGgcg                                                        <  2:2798120/101‑1 (MQ=255)


                                         cgcgCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGgcg                                                        >  1:1003240/1‑101 (MQ=255)


                                           cgcTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGcgcg                                                      >  1:3794447/1‑101 (MQ=255)


                                           cgcTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGcgcg                                                      >  2:1403065/1‑101 (MQ=255)


                                            gcTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCgcgg                                                     >  2:884541/1‑101 (MQ=255)


                                             cTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCgcggg                                                    <  1:2215015/101‑1 (MQ=255)


                                               cgggcgggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCgcgggcg                                                  <  1:4191655/101‑1 (MQ=255)


                                                gggggggACGCGGAAGGCCCCGGTCCTGTCGGTCGCGGTCGGGATCGCGGCGAATTGCCCCGCCGGGGCCGTGACCTTCGCCGCCATCCAGGCgcgggcgg                                                 <  2:1551024/101‑1 (MQ=255)


                                                gggcgggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCgcgggcgg                                                 >  2:3612830/1‑101 (MQ=255)


                                                gggcgggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCgcgggcgg                                                 >  1:3908620/1‑101 (MQ=255)


                                                  gcgggATGCCGAGAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGtt                                               >  1:3330730/1‑101 (MQ=255)


                                                  gcgggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGtt                                               <  1:568097/101‑1 (MQ=255)


                                                  gcgggATGCCGAAAGCCGCCGTCCTGGCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGtt                                               <  1:842208/101‑1 (MQ=255)


                                                   ggggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGttt                                              <  1:1831828/100‑1 (MQ=255)


                                                    gggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTc                                             >  1:1656837/1‑101 (MQ=255)


                                                    gggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTc                                             <  2:1474875/101‑1 (MQ=255)


                                                     ggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCg                                            >  1:3813968/1‑101 (MQ=255)


                                                      gATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCgg                                           >  2:3212206/1‑101 (MQ=255)


                                                      gATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCgg                                           >  1:1813291/1‑101 (MQ=255)


                                                        tGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCa                                         <  2:1803669/101‑1 (MQ=255)


                                                         gCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCAt                                        >  1:2867887/1‑101 (MQ=255)


                                                         gCCGAAAGCCGCCGTCCTGTCGGTCGCGGCCGAGATCGAGGCGAATTGCGCCACCGGGACCGTGGCCTTCGCCGCCCTCCAGGCGCGGGCGGATTGGGCAt                                        >  2:1306688/1‑101 (MQ=255)


                                                          ccGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCAtt                                       <  2:3822850/101‑1 (MQ=255)


                                                           cGAAAGCCGCCGTCCTGTCGGTAGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTg                                      <  1:3967845/101‑1 (MQ=255)


                                                            gAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTgg                                     >  2:1982034/1‑101 (MQ=255)


                                                            gAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTgg                                     >  1:768300/1‑101 (MQ=255)


                                                             aaaGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGt                                    <  2:1813165/101‑1 (MQ=255)


                                                              aaGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTc                                   >  2:699064/1‑101 (MQ=255)


                                                                  cgccgTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCg                               <  2:1492883/101‑1 (MQ=255)


                                                                  cgccgTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCg                               <  1:162854/101‑1 (MQ=255)


                                                                   gccgTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGt                              <  1:4269221/101‑1 (MQ=255)


                                                                    ccgTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGtc                             <  1:3407031/101‑1 (MQ=255)


                                                                       tCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCg                          <  2:1625163/101‑1 (MQ=255)


                                                                        ccTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGa                         >  2:3705312/1‑101 (MQ=255)


                                                                        ccTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGa                         <  2:1765134/101‑1 (MQ=255)


                                                                          tGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATc                       <  2:640675/101‑1 (MQ=255)


                                                                          tGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATc                       >  2:3419011/1‑101 (MQ=255)


                                                                          tGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATc                       <  2:4120575/101‑1 (MQ=255)


                                                                           gtcggtcgCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCg                      <  2:4262035/101‑1 (MQ=255)


                                                                           gtcggtcgCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCg                      >  2:248685/1‑101 (MQ=255)


                                                                             cggtcgcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCTGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgtg                    <  2:3917774/101‑1 (MQ=255)


                                                                             cggtcgcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgtg                    <  2:172889/101‑1 (MQ=255)


                                                                              ggtcgcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgtgg                   <  1:3810528/101‑1 (MQ=255)


                                                                              ggtcgcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgtgg                   <  2:3794162/101‑1 (MQ=255)


                                                                                   cggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAg              <  2:4006638/101‑1 (MQ=255)


                                                                                   cggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAg              >  1:1736095/1‑101 (MQ=255)


                                                                                     gtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGt            >  1:1101094/1‑101 (MQ=255)


                                                                                     gtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGt            >  2:3999411/1‑101 (MQ=255)


                                                                                     gtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGt            >  2:28679/1‑101 (MQ=255)


                                                                                      tcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTc           <  2:3936911/101‑1 (MQ=255)


                                                                                      tcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTc           >  1:2398292/1‑101 (MQ=255)


                                                                                      tcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGCGAAgggc           >  1:2816481/1‑99 (MQ=255)


                                                                                       cgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCt          >  2:2549490/1‑101 (MQ=255)


                                                                                        gagaTCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCtt         >  1:3745695/1‑101 (MQ=255)


                                                                                        gagaTCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCtt         >  1:784922/1‑101 (MQ=255)


                                                                                         agaTCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTg        >  1:3352532/1‑101 (MQ=255)


                                                                                          gaTCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTgg       >  1:3104407/1‑101 (MQ=255)


                                                                                           aTCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTGGa      >  1:741442/1‑101 (MQ=255)


                                                                                               cgGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTGGACGcc  <  2:2634507/101‑1 (MQ=255)


GACCGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGAGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTGGACGCC  >  NC_014034/2579395‑2579590

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG