Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,590,068 C→G 100% V12L (GTT→CTT)  glnE ← glutamate‑ammonia‑ligase adenylyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,590,0680C→G100.0% 594.6 158V12L (GTT→CTT) glnEglutamate‑ammonia‑ligase adenylyltransferase
Reads supporting (aligned to +/- strand):  new base (65/93):  ref base (0/0):  total (65/93)

Only 100 of 168 total aligned reads displayed.

CGAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAACGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTT  >  NC_014034/2589970‑2590165
                                                                                                  |                                                                                                 
cGAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGgg                                                                                                 >  1:317373/1‑101 (MQ=255)
cGAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGgg                                                                                                 >  2:3910546/1‑101 (MQ=255)
 gAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGggg                                                                                                <  1:847880/101‑1 (MQ=255)
 gAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGggg                                                                                                >  1:1001288/1‑101 (MQ=255)
  aaCAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGc                                                                                               >  1:1473005/1‑101 (MQ=255)
  aaCAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGc                                                                                               <  2:1995012/101‑1 (MQ=255)
   aCAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCg                                                                                              >  2:550500/1‑101 (MQ=255)
    cAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGt                                                                                             >  2:748678/1‑101 (MQ=255)
     aGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTg                                                                                            <  1:4625713/101‑1 (MQ=255)
     aGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTg                                                                                            >  1:2757938/1‑101 (MQ=255)
        cagccgTGCCCGCGATCATACCCCGGATTTCGGGCGGAAAATCGCCATAACGGTCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCgg                                                                                         <  2:3808399/99‑1 (MQ=255)
         cgccgTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGTCAACGATATCTGCGCCACGGGTCGTATCAAGGGGAAGGGGCGTGCggg                                                                                        >  2:4636986/1‑101 (MQ=255)
         cgccgTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCggg                                                                                        <  1:3815357/101‑1 (MQ=255)
          gccgTGCCCGCGATCACCCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGt                                                                                       <  2:2990526/101‑1 (MQ=255)
          gccgTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGACGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGt                                                                                       >  1:3937554/1‑101 (MQ=255)
           ccgTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTc                                                                                      <  2:3307788/101‑1 (MQ=255)
           ccgTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTc                                                                                      <  2:293658/101‑1 (MQ=255)
            cgTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCa                                                                                     <  2:1268649/101‑1 (MQ=255)
            cgTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCa                                                                                     <  1:550548/101‑1 (MQ=255)
             gTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAg                                                                                    <  1:2017170/101‑1 (MQ=255)
             gTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAg                                                                                    <  1:1036818/101‑1 (MQ=255)
              tGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAgg                                                                                   <  2:267786/101‑1 (MQ=255)
              tGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAgg                                                                                   <  2:3399579/101‑1 (MQ=255)
              tGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAgg                                                                                   <  2:4570976/101‑1 (MQ=255)
                   gcgATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGa                                                                              <  2:1881380/101‑1 (MQ=255)
                     gATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATg                                                                            >  1:718020/1‑101 (MQ=255)
                     gATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATg                                                                            <  2:896605/101‑1 (MQ=255)
                      aTCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGc                                                                           <  1:1805268/101‑1 (MQ=255)
                      aTCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGc                                                                           <  2:4232073/101‑1 (MQ=255)
                         aaCCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAg                                                                        <  2:163780/101‑1 (MQ=255)
                         aaCCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAg                                                                        >  2:3236877/1‑101 (MQ=255)
                            ccccGGATTTCGGGCGGAAAATCGCCACAACGGGCGACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCt                                                                     <  1:1952682/101‑1 (MQ=255)
                                ggATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATg                                                                 >  1:634330/1‑101 (MQ=255)
                                ggATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATg                                                                 <  2:2689685/101‑1 (MQ=255)
                                ggATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATg                                                                 >  1:2137864/1‑101 (MQ=255)
                                 gATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATgg                                                                >  1:3339307/1‑101 (MQ=255)
                                  aTTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGgc                                                               <  2:4038913/101‑1 (MQ=255)
                                     tCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGcc                                                            <  1:4237196/101‑1 (MQ=255)
                                      cGGGCGGAAAATCGCCATAACGGGCAACGAGATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGCGATGCAAGGCTCATGGCGCCa                                                           <  1:3435178/101‑1 (MQ=255)
                                      cGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCa                                                           <  1:459636/101‑1 (MQ=255)
                                      cGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCa                                                           >  1:4067435/1‑101 (MQ=255)
                                       gggCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGGCAGGCGGGATGCAAGGCTCATGGCGCCAg                                                          >  1:4310810/1‑101 (MQ=255)
                                       gggCGGAAAATCGCCATAACGGGCAACGACATCGGCCCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAg                                                          <  1:1339619/101‑1 (MQ=255)
                                         gCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGAc                                                        <  1:1740625/101‑1 (MQ=255)
                                         gCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGAc                                                        >  1:1582586/1‑101 (MQ=255)
                                          cGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACt                                                       <  1:3917503/101‑1 (MQ=255)
                                           ggAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTa                                                      >  1:509070/1‑101 (MQ=255)
                                            gAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAg                                                     <  1:1417596/101‑1 (MQ=255)
                                            gAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAg                                                     >  1:3097569/1‑101 (MQ=255)
                                             aaaaTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGGTCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCGCAGGGAGCCAGACGAgc                                                    >  1:2250657/1‑101 (MQ=255)
                                              aaaTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAgcc                                                   <  1:4403882/101‑1 (MQ=255)
                                              aaaTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAgcc                                                   <  2:1176856/101‑1 (MQ=255)
                                                aTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAgccgc                                                 <  2:317341/101‑1 (MQ=255)
                                                   gCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCcgccgc                                              >  2:385683/1‑101 (MQ=255)
                                                   gCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCcgccgc                                              <  1:2891428/101‑1 (MQ=255)
                                                   gCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCcgccgc                                              >  2:192114/1‑101 (MQ=255)
                                                     cATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCaa                                            <  1:3865613/101‑1 (MQ=255)
                                                      aTAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAg                                           >  1:3464129/1‑101 (MQ=255)
                                                       tAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGc                                          <  1:2980314/101‑1 (MQ=255)
                                                        aaCGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGcc                                         <  1:2252223/101‑1 (MQ=255)
                                                         aCGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGccc                                        <  2:584965/101‑1 (MQ=255)
                                                            ggCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCgg                                     <  2:85944/101‑1 (MQ=255)
                                                             gCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCggg                                    <  2:2457323/101‑1 (MQ=255)
                                                              cAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCgggc                                   >  1:891247/1‑100 (MQ=255)
                                                              cAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCgggc                                   >  1:3718657/1‑100 (MQ=255)
                                                              cAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGt                                   >  1:2162123/1‑101 (MQ=255)
                                                               aaCGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTc                                  <  1:2077280/101‑1 (MQ=255)
                                                               aaCGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTc                                  <  1:3679869/101‑1 (MQ=255)
                                                               aaCGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTc                                  <  2:1582482/101‑1 (MQ=255)
                                                               aaCGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTc                                  <  2:1001205/101‑1 (MQ=255)
                                                               aaCGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGACAGACTAGCCGCCGCAAGCCCCGGGTc                                  >  2:53545/1‑101 (MQ=255)
                                                                aCGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCa                                 >  1:1615634/1‑101 (MQ=255)
                                                                 cGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGCGATGCAAGGCTCATGGTGCCAGACTAGCCGCCGCAAGCCCCGGGTCaa                                >  2:4411360/1‑101 (MQ=255)
                                                                   aCATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAgg                              <  2:1963381/101‑1 (MQ=255)
                                                                       cGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGgcgcg                          <  1:3237122/101‑1 (MQ=255)
                                                                        ggCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGgcgcgc                         >  1:1785176/1‑101 (MQ=255)
                                                                        ggCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGgcgcgc                         >  1:969278/1‑101 (MQ=255)
                                                                          cgcCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCaa                       <  2:2179895/101‑1 (MQ=255)
                                                                             cgggggCCGGAGCAAGGGGAAGGGGCGTGCGGGTCGGGCGGGATGCAGGGCTCATGGCGCCAGATTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATg                    <  2:2250495/98‑1 (MQ=255)
                                                                              ccGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCTCGGGTCAAGGCGCGCAAATgc                   <  2:3350236/100‑1 (MQ=255)
                                                                                gggCCGGATCAAGGGGAAGGGGTTTGCGGTTCAGGCGGTATGGAAGTCTCATGGGGCCAGCCTACCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccc                 <  1:4411652/101‑1 (MQ=255)
                                                                                 ggCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgcccc                <  1:2722134/101‑1 (MQ=255)
                                                                                 ggCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgcccc                <  2:1782403/101‑1 (MQ=255)
                                                                                 ggCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgcccc                >  2:3093741/1‑101 (MQ=255)
                                                                                  gCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccg               <  1:2566424/101‑1 (MQ=255)
                                                                                    cGGATCAAGGGGAAGGGGGGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccgcc             <  1:2137938/101‑1 (MQ=255)
                                                                                    cGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccgcc             >  1:566889/1‑101 (MQ=255)
                                                                                      gATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccgcccc           <  1:1868059/101‑1 (MQ=255)
                                                                                       aTCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCAGCCCCt          <  1:3265581/101‑1 (MQ=255)
                                                                                        tCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTg         <  2:1351868/101‑1 (MQ=255)
                                                                                          aaGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTc       >  2:642517/1‑101 (MQ=255)
                                                                                          aaGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTc       >  2:3922486/1‑101 (MQ=255)
                                                                                           aGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCa      <  1:4122533/101‑1 (MQ=255)
                                                                                            ggggAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCAt     <  2:1785057/101‑1 (MQ=255)
                                                                                             gggAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGtagat    >  2:3675273/1‑97 (MQ=255)
                                                                                             gggAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATc    >  1:720258/1‑101 (MQ=255)
                                                                                              ggAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCt   >  2:711219/1‑101 (MQ=255)
                                                                                               gAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCtt  >  2:4299486/1‑101 (MQ=255)
                                                                                               gAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCtt  <  1:1485634/101‑1 (MQ=255)
                                                                                               gAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCtt  >  1:2853181/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CGAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAACGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTT  >  NC_014034/2589970‑2590165

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG