Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,595,353 G→C 100% V205V (GTG→GTC bglX → periplasmic beta‑glucosidase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,595,3530G→C100.0% 676.1 191V205V (GTG→GTCbglXperiplasmic beta‑glucosidase
Reads supporting (aligned to +/- strand):  new base (90/100):  ref base (0/1):  total (90/101)

Only 100 of 205 total aligned reads displayed.

GCGGATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTGGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCCGCGATCA  >  NC_014034/2595255‑2595450
                                                                                                  |                                                                                                 
atgcaTGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGa                                                                                                 >  2:401935/5‑101 (MQ=255)
 cGGATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAg                                                                                                >  1:4518356/1‑101 (MQ=255)
 cGGATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAg                                                                                                >  1:3844354/1‑101 (MQ=255)
 cGGATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAg                                                                                                <  2:2340166/101‑1 (MQ=255)
   gATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGc                                                                                              <  2:3548736/101‑1 (MQ=255)
         gACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGccc                                                                                        >  1:2985681/1‑101 (MQ=255)
         gACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGccc                                                                                        >  1:1906489/1‑101 (MQ=255)
         gACATGGCCCGGCCCGACCGGATGATGCCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGccc                                                                                        <  2:2275768/101‑1 (MQ=255)
           cagggcccggcccGACCGGATGATGGCGCCCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCcg                                                                                      <  1:55941/98‑1 (MQ=255)
           cATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGGCCcg                                                                                      >  1:1512088/1‑101 (MQ=255)
           cATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCcg                                                                                      <  2:1723774/101‑1 (MQ=255)
            aTGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCACCGGGCGCGGTTATGCGGCGGTCGAGGCGAGCCCcgc                                                                                     >  2:1761676/1‑101 (MQ=255)
            aTGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGGCCATCGCCGGGCGCGTTTTTGAGGCGGGCGAGGCGAGCCCcgc                                                                                     >  1:3555020/1‑101 (MQ=255)
             tGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCcgcg                                                                                    >  1:1765644/1‑101 (MQ=255)
             tGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCcgcg                                                                                    <  1:3257263/101‑1 (MQ=255)
             tGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCcgcg                                                                                    >  1:2852419/1‑101 (MQ=255)
              ggcccggcccGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGa                                                                                   >  2:3743579/1‑101 (MQ=255)
              ggcccggcccGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGa                                                                                   >  1:1743598/1‑101 (MQ=255)
               gcccggcccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGAc                                                                                  <  2:69515/101‑1 (MQ=255)
               gcccggcccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGAc                                                                                  <  2:3501510/101‑1 (MQ=255)
                   ggcccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTg                                                                              >  1:2005737/1‑101 (MQ=255)
                   ggcccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTg                                                                              >  2:3174711/1‑101 (MQ=255)
                   ggcccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTg                                                                              >  2:2726590/1‑101 (MQ=255)
                     cccgCCCGGATGATGGCGGCCGTGAAGCATTTCGCGGGGTACGGCCTGTCCATCGCCGGGCGAGATAATGCGGCGGTCGGGGCGAGCCCCGCGACGCTGAt                                                                            <  2:2509353/101‑1 (MQ=255)
                     cccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGAt                                                                            <  1:4312834/101‑1 (MQ=255)
                     cccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGAt                                                                            <  1:2820775/101‑1 (MQ=255)
                     cccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGAt                                                                            <  2:3221676/101‑1 (MQ=255)
                       cgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGc                                                                          <  1:343920/101‑1 (MQ=255)
                        gACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCg                                                                         <  1:4593626/101‑1 (MQ=255)
                        gACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCg                                                                         >  2:4655540/1‑101 (MQ=255)
                        gACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCg                                                                         >  1:379034/1‑101 (MQ=255)
                         aCCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCgg                                                                        <  2:2242650/101‑1 (MQ=255)
                         aCCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCgg                                                                        <  2:827185/101‑1 (MQ=255)
                         aCCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCgg                                                                        <  2:3958417/101‑1 (MQ=255)
                          ccGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGa                                                                       <  2:2820052/101‑1 (MQ=255)
                          ccGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGa                                                                       <  2:2417684/101‑1 (MQ=255)
                           cGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGAt                                                                      <  1:4133806/101‑1 (MQ=255)
                           cGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGAt                                                                      >  2:3839475/1‑101 (MQ=255)
                           cGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGAt                                                                      >  2:2404742/1‑101 (MQ=255)
                            ggATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATc                                                                     >  1:2907455/1‑101 (MQ=255)
                             gatgatGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCa                                                                    <  2:4407239/101‑1 (MQ=255)
                             gataatAGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCa                                                                    <  1:2541214/101‑1 (MQ=255)
                              atgatgGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCAt                                                                   >  1:3738457/1‑101 (MQ=255)
                              atgatgGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCAt                                                                   >  2:1217614/1‑101 (MQ=255)
                               tgatgGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATg                                                                  >  1:476528/1‑101 (MQ=255)
                                     cGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGccc                                                            <  1:1139642/101‑1 (MQ=255)
                                      gACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCt                                                           >  2:2362613/1‑101 (MQ=255)
                                      gACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCt                                                           <  2:4505916/101‑1 (MQ=255)
                                      gACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCt                                                           >  2:8968/1‑101 (MQ=255)
                                        cccTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTc                                                         <  2:2874854/101‑1 (MQ=255)
                                        cccTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTc                                                         <  1:3145987/101‑1 (MQ=255)
                                         ccTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCa                                                        <  1:2241755/101‑1 (MQ=255)
                                         ccTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCa                                                        >  1:2122949/1‑101 (MQ=255)
                                          cTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGATCCCTTCaa                                                       >  2:802221/1‑101 (MQ=255)
                                          cTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCaa                                                       >  2:2322560/1‑101 (MQ=255)
                                           tGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCaaa                                                      <  2:3685429/101‑1 (MQ=255)
                                            gAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAAt                                                     >  1:4210808/1‑101 (MQ=255)
                                            gAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAAt                                                     <  2:1453767/101‑1 (MQ=255)
                                            gAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAAt                                                     <  1:3676961/101‑1 (MQ=255)
                                             aaGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATc                                                    <  1:2336487/101‑1 (MQ=255)
                                              aGCATTTCGCGGGCTGCGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCg                                                   <  2:416292/101‑1 (MQ=255)
                                               gCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCgg                                                  >  2:2041994/1‑101 (MQ=255)
                                                cATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCggg                                                 <  2:1949838/101‑1 (MQ=255)
                                                cATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCggg                                                 >  2:1493889/1‑101 (MQ=255)
                                                   ttCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGt                                              <  1:2398974/101‑1 (MQ=255)
                                                    tCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTg                                             <  2:2985452/101‑1 (MQ=255)
                                                    tCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTg                                             <  1:1717663/101‑1 (MQ=255)
                                                    tCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTg                                             <  1:4390627/101‑1 (MQ=255)
                                                       cgGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTggcg                                          >  1:3011259/1‑101 (MQ=255)
                                                          gCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGgcggcg                                       <  2:3285738/101‑1 (MQ=255)
                                                            tACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGGCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTCGCGGCggg                                     >  2:4298305/1‑101 (MQ=255)
                                                             aCGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCgggg                                    <  1:3203230/101‑1 (MQ=255)
                                                              cGGCCTGTCCATCGCCGGGCGCGATTATTCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCCGATCAGGGCGGCCTTCAAATCAGGCGTGGAGGCggggg                                   >  1:210546/1‑101 (MQ=255)
                                                              cGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCggggg                                   <  2:4383662/101‑1 (MQ=255)
                                                                gCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGcc                                 >  1:288510/1‑101 (MQ=255)
                                                                gCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGcc                                 <  2:3458412/101‑1 (MQ=255)
                                                                gCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGcc                                 <  2:3656981/101‑1 (MQ=255)
                                                                gCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGcc                                 <  1:1432320/101‑1 (MQ=255)
                                                                gCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGcc                                 >  1:1315620/1‑101 (MQ=255)
                                                                  cTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcgg                               >  2:2891663/1‑101 (MQ=255)
                                                                  cTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcgg                               <  2:1872973/101‑1 (MQ=255)
                                                                   tGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggg                              >  2:2163447/1‑100 (MQ=255)
                                                                    gTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggtg                             >  1:815541/1‑101 (MQ=255)
                                                                    gTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggtg                             <  2:4080311/101‑1 (MQ=255)
                                                                     tCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggggc                            >  2:890229/1‑101 (MQ=255)
                                                                      ccATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggtgcg                           >  1:1869467/1‑101 (MQ=255)
                                                                      ccATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggtgcg                           <  1:2201584/101‑1 (MQ=255)
                                                                      ccATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcgggggg                           >  1:4465778/1‑97 (MQ=255)
                                                                         tCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggtgcggtg                        <  1:2282288/101‑1 (MQ=255)
                                                                           gCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGAt                      <  1:1045467/101‑1 (MQ=255)
                                                                              gggCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGGGCGGTGAtggt                   >  2:2456628/1‑101 (MQ=255)
                                                                                gcgcGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGGGCGGTGAtggtgg                 >  1:1970697/1‑101 (MQ=255)
                                                                                gcgcGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGATGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGGCGAGGCGGGGAaggggg                 >  2:4562593/1‑101 (MQ=255)
                                                                                     aTTATGCGGCGGGCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCttt            <  1:401972/101‑1 (MQ=255)
                                                                                        aTGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTcc         <  1:1276603/101‑1 (MQ=255)
                                                                                           aggcggTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCcggg      >  2:1334683/2‑99 (MQ=255)
                                                                                            ggcggTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCCGCGa     >  2:104898/1‑101 (MQ=255)
                                                                                              cggTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCCGCGATc   <  1:889097/101‑1 (MQ=255)
                                                                                               ggTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCCGCGATCa  >  1:4152255/1‑101 (MQ=255)
                                                                                               ggTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCCGCGATCa  >  1:2682302/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GCGGATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTGGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCCGCGATCA  >  NC_014034/2595255‑2595450

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG