Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,174 G→A 100% intergenic (‑150/+126) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1740G→A100.0% 331.2 123intergenic (‑150/+126)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (40/77):  ref base (0/2):  total (40/83)

Only 100 of 136 total aligned reads displayed.

GCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GG.C.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCC  >  NC_014034/2640078‑2640272
                                                                                                     |                                                                                                    
gcgcCGGGTATGCGACGCGGCTGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCAccc                                                                                                   <  2:2196345/101‑1 (MQ=37)
gcgcCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCAccc                                                                                                   <  1:566148/101‑1 (MQ=255)
gcgcCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCAccc                                                                                                   <  2:1043641/101‑1 (MQ=255)
  gcCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGGTGCCCCCCT.CTTT.GC.C.TTCGGCCAATTCCccccc                                                                                                 >  1:2171036/1‑101 (MQ=255)
   ccGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCAcccccc                                                                                                <  2:2761747/101‑1 (MQ=255)
     gggTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGa                                                                                              <  2:1414109/101‑1 (MQ=255)
      ggTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAg                                                                                             <  2:1554785/101‑1 (MQ=255)
       gTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAgg                                                                                            <  2:2171866/101‑1 (MQ=255)
       gTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAgg                                                                                            <  2:3356069/101‑1 (MQ=255)
         aTGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTa                                                                                          <  2:3438448/101‑1 (MQ=255)
           gCGACGCGGCGGGAGGCGTGTCAACCGGGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTAtt                                                                                        <  1:1434330/101‑1 (MQ=25)
           gCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTAtt                                                                                        <  2:756326/101‑1 (MQ=255)
                    cggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TTGGG.C.TTCGGCCCATTCACCCCCCCAGGGATTTTGaggaag                                                                               >  2:3682733/1‑96 (MQ=25)
                     ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGa                                                                              <  1:345314/101‑1 (MQ=37)
                      gccagCGTGTGAACCGTGGCGCTTTTGCTCAGGCGAAAGGGGGGCTGTGCCCCCCT.C.TT.GGCC.TTCGGGCAATTCACCCCCCGAGGTATTTGGAGCAAGAt                                                                             <  2:513021/97‑1 (MQ=21)
                      gcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGAt                                                                             <  1:1820549/101‑1 (MQ=37)
                      gcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGAt                                                                             <  2:3801667/101‑1 (MQ=37)
                       cAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATg                                                                            <  2:1805999/101‑1 (MQ=37)
                       cAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATg                                                                            <  1:2232739/101‑1 (MQ=25)
                        aGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGa                                                                           <  2:2367902/101‑1 (MQ=37)
                         ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaa                                                                          <  1:2184471/101‑1 (MQ=25)
                         ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCC.C.TT.GG.CGCTTGGGCCATTTACCCCCCCAGGTATTTGGAGGAAGATGaa                                                                          >  1:2089534/1‑101 (MQ=255)
                          gCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                         <  1:1897055/101‑1 (MQ=25)
                           cGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGGGCTGCCCCCCT.C.TTCTC.C.TTCGGCCAATTCACCCCCCCCGGTATTTTGAGCAAGATGaaaa                                                                        >  2:1682965/1‑101 (MQ=25)
                           cGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaag                                                                        <  1:58641/101‑2 (MQ=25)
                              gtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaagacc                                                                     <  1:1109101/101‑5 (MQ=25)
                                  aCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg..g                                                              <  1:1652240/101‑1 (MQ=25)
                                      tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TTCGT.C.TTTGGCCAATTCAGCCTCCGAGGTATTTTTGCCAAGATG.CAAGAGCATG..GGTTc                                                          >  2:3141611/1‑101 (MQ=16)
                                      tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTc                                                          <  2:553602/101‑1 (MQ=21)
                                      gggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTc                                                          <  2:2139839/100‑1 (MQ=25)
                                       ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCa                                                         <  2:2789973/101‑1 (MQ=25)
                                        gcgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCAt                                                        <  2:898430/101‑1 (MQ=25)
                                           cTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCtt                                                     <  1:3304586/101‑1 (MQ=21)
                                           cTTGTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCtt                                                     <  1:2347939/101‑1 (MQ=25)
                                            ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTg                                                    <  2:2517352/101‑1 (MQ=25)
                                               tGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGcgt                                                 <  2:65143/101‑3 (MQ=21)
                                                gcgcAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TTAGC.C.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTc                                                <  1:4563587/101‑1 (MQ=25)
                                                gcgcAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTc                                                <  2:2584354/101‑1 (MQ=25)
                                                  gcAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCaa                                              <  2:843951/101‑1 (MQ=25)
                                                   gccccgaaaAGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAat                                            <  2:2641040/95‑1 (MQ=14)
                                                    agccAAAAGGGGGCCGCTGCCCCCCT.C.TT.GGTC.TTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAat                                            <  2:746158/98‑1 (MQ=25)
                                                     ggCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAata                                           <  2:3575984/101‑1 (MQ=21)
                                                     cccgaaaAGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAatat                                          <  2:2473130/97‑1 (MQ=17)
                                                       cgaaaAGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATcc                                        <  2:575675/99‑1 (MQ=17)
                                                       cgaaaAGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATcc                                        >  1:1204884/3‑101 (MQ=17)
                                                       cgaaaAGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATcc                                        <  1:4234149/99‑1 (MQ=17)
                                                       cgaaaAGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATcc                                        >  2:4119652/3‑101 (MQ=17)
                                                        gaaaAGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATccc                                       <  1:1215644/100‑1 (MQ=17)
                                                        gaaaAGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTANCTTGCCCCAAATATccc                                       >  2:1778669/2‑101 (MQ=17)
                                                         aaaGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATccc                                       <  1:518442/101‑1 (MQ=25)
                                                         aaaGGGGGGCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATccc                                       <  2:3189647/101‑1 (MQ=21)
                                                         aaaGGGGGGC.CCGCCCCCCTCC.TT.GG.C.CTCCGGCAAATCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCg                                      <  1:3006725/101‑1 (MQ=21)
                                                         aaaAGGGGGCCCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCg                                      <  1:258985/101‑1 (MQ=18)
                                                            agggggCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggg                                   >  1:1356112/2‑101 (MQ=18)
                                                             gggggCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCgggg                                   <  2:1352989/101‑1 (MQ=25)
                                                             gggggCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCgggg                                   <  2:2731094/101‑1 (MQ=25)
                                                             gggggCGCTG.CCCCCT.C.TT.GGCC.TTTGGCCAATTC.ACCCCTGGGATATTTCGGGCAAGATGAA...ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggggg                                 <  2:2120767/101‑1 (MQ=2)
                                                               gggCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtg                                <  2:2966740/101‑3 (MQ=18)
                                                               gggCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtg                                >  2:3699403/1‑99 (MQ=18)
                                                               gggCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtg                                >  2:4392741/1‑99 (MQ=18)
                                                               gggCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtg                                >  1:4408096/1‑99 (MQ=18)
                                                                ggCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtga                               <  1:4092162/101‑4 (MQ=21)
                                                                ggCGCTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGa                                <  2:1026362/101‑1 (MQ=25)
                                                                gccgcTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtga                               <  1:1084325/99‑4 (MQ=21)
                                                                 gcgcTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgag                              <  2:4149903/101‑5 (MQ=21)
                                                                  cgcTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCGCCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgagg                             <  2:3939473/101‑6 (MQ=21)
                                                                  cgcTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgagg                             <  2:2002466/101‑6 (MQ=21)
                                                                  cgcTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgagg                             <  2:240521/101‑6 (MQ=21)
                                                                   gcTGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgaggc                            >  1:3459872/1‑95 (MQ=21)
                                                                    ttGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                            <  1:2032567/100‑1 (MQ=25)
                                                                     tGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                           <  1:3683008/101‑1 (MQ=25)
                                                                     tGCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCCGGAGATTTGCGGCAAGGTGAAAGGA..TGG..GGTATATCTTGCCCCCAATATCCCgagggtgcggcga                          >  2:317634/1‑93 (MQ=18)
                                                                      gCCCCCCT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggggaggcgaa                         >  1:127641/1‑93 (MQ=25)
                                                                       ccccccT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGa                         >  2:1541300/1‑101 (MQ=25)
                                                                       ccccccT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGa                         >  2:1899673/1‑101 (MQ=25)
                                                                          cccT.C.TT.GGCC.TTCGGTCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGTCTCAAATATCCCGGGGGGAGTCCTGAAAg                      <  1:3644658/101‑1 (MQ=25)
                                                                          cccT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                      >  1:2889481/1‑101 (MQ=37)
                                                                          cccT.C.TT.GGAC.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                      <  1:2032281/101‑1 (MQ=37)
                                                                           ccT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                     >  1:462203/1‑101 (MQ=37)
                                                                           ccT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                     <  1:1402267/101‑1 (MQ=37)
                                                                           ccT.C.TT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                     <  1:3501361/101‑1 (MQ=37)
                                                                            ct.c.tT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                    >  2:3327683/1‑101 (MQ=37)
                                                                             t.c.tT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                   <  1:1242583/101‑1 (MQ=37)
                                                                             t.c.tT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                   >  1:3813506/1‑101 (MQ=37)
                                                                             t.c.tT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                   <  1:2882076/101‑1 (MQ=37)
                                                                                   tggcc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                >  2:1307922/5‑101 (MQ=37)
                                                                                     gcc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg              <  2:1989697/99‑1 (MQ=37)
                                                                                      cc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg             >  1:4620347/2‑101 (MQ=37)
                                                                                       c.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc            >  1:4438987/1‑101 (MQ=37)
                                                                                         ttCGGACAAGTCACCCCCCGAGGTATTTGGGGCAAGATGAAAA.ACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa           <  2:1841463/101‑1 (MQ=25)
                                                                                          tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag          >  2:1080611/1‑101 (MQ=37)
                                                                                            ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag        <  1:1063811/101‑1 (MQ=255)
                                                                                            ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag        >  1:1294296/1‑101 (MQ=37)
                                                                                             gCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc       <  1:3351117/101‑1 (MQ=255)
                                                                                             gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc       >  2:158525/1‑101 (MQ=37)
                                                                                              ccAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc      >  1:2359969/1‑101 (MQ=37)
                                                                                               cAATTCACCCACCGAGGGATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc     >  2:2318962/1‑101 (MQ=25)
                                                                                                aaTTCACCACCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCCTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc    >  2:1652292/1‑101 (MQ=37)
                                                                                                 aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc   >  2:2746508/1‑101 (MQ=37)
                                                                                                 aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc   >  1:4191721/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
GCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.C.TT.GG.C.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCC  >  NC_014034/2640078‑2640272

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG